Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	3,489,219	G→A	intergenic (‑13/+4)	glpR ← / ← glpG	DNA‑binding transcriptional repressor/predicted intramembrane serine protease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	3,489,219	0	G	A	100.0%	267.3 / NA	66	intergenic (‑13/+4)	glpR/glpG	DNA‑binding transcriptional repressor/predicted intramembrane serine protease
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (37/29);  total (37/29)

GACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTCTAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCTAACCCGGCG  >  REL606/3489147‑3489289                                                                         |                                                                       gACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTCTAAATCCCTAGaa                                                                     >  2:856307/1‑76 (MQ=255)  acacAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTCTAAATCCCTAGAat                                                                    <  1:993944/76‑1 (MQ=255)     tAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTCTAAATCCCTAGAattat                                                                 >  1:207197/1‑76 (MQ=255)     tAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTC‑TTTTTTAATTCCTTGAattatt                                                                >  1:1606015/1‑76 (MQ=255)      aaCCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTCTAAATCCCTAGAattatt                                                                >  1:2027576/1‑76 (MQ=255)      aaCCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTCTAAATCCCTAGAattatt                                                                >  1:1502853/1‑76 (MQ=255)               gTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTCTAAATCCCTAGAATTAttttcgttttc                                                       >  2:1344312/1‑76 (MQ=255)                tttAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTCTAAATCCCTAGAATTAttttcgttttcg                                                      >  1:74731/1‑76 (MQ=255)                  tAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTCTAAATCCCTAGAATTATTTTCGTTTTcgcg                                                    <  2:1036637/76‑1 (MQ=255)                  tAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTCTAAATCCCTAGAATTATTTTCGTTTTcgcg                                                    <  2:814899/76‑1 (MQ=255)                  tAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTCTAAATCCCTAGAATTATTTTCGTTTTcgcg                                                    <  2:814997/76‑1 (MQ=255)                   aaCCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTCTAAATCCCTAGAATTATTTTCGTTTTCgcgc                                                   >  1:1154193/1‑76 (MQ=255)                    aCCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTCTAAATCCCTAGAATTATTTTCGTTTTCGCGCa                                                  <  2:1937974/76‑1 (MQ=255)                       aGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTCTAAATCCCTAGAATTATTTTCGTTTTCGCGCATTg                                               >  2:1080282/1‑76 (MQ=255)                       aGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTCTAAATCCCTAGAATTATTTTCGTTTTCGCGCATTg                                               >  2:357186/1‑76 (MQ=255)                         ttCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTCTAAATCCCTAGAATTATTTTCGTTTTCGCGCATTGAg                                             <  2:1683921/76‑1 (MQ=255)                          tCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTCTAAATCCCTAGAATTATTTTCGTTTTCGCGCATTGAGc                                            >  1:519913/1‑76 (MQ=255)                          tCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTCTAAATCCCTAGAATTATTTTCGTTTTCGCGCATTGAGc                                            <  1:1466202/76‑1 (MQ=255)                           cGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTCTAAATCCCTAGAATTATTTTCGTTTTCGCGCATTGAGCg                                           <  1:2023229/76‑1 (MQ=255)                            gATAATACCGTTGTGACGTTGTGTTTGTTTCATTTCTAAATCCCTAGAATTATTTTCGTTTTCGCGCATTGAGCGa                                          >  1:175576/1‑76 (MQ=255)                             ataataCCGTTGTGACGTTGTGTTTGTTTCATTTCTAAATCCCTAGAATTATTTTCGTTTTCGCGCATTGAGCGaa                                         >  2:2039678/1‑76 (MQ=255)                             ataataCCGTTGTGACGTTGTGTTTGTTTCATTTCTAAATCCCTAGAATTATTTTCGTTTTCGCGCATTGAGCGaa                                         <  2:1953645/76‑1 (MQ=255)                              taataCCGTTGTGACGTTGTGTTTGTTTCATTTCTAAATCCCTAGAATTATTTTCGTTTTCGCGCATTGAGCGAAt                                        >  2:1149098/1‑76 (MQ=255)                               aataCCGTTGTGACGTTGTGTTTGTTTCATTTCTAAATCCCTAGAATTATTTTCGTTTTCGCGCATTGAGCGAATc                                       >  1:202429/1‑76 (MQ=255)                                 taCCGTTGTGACGTTGTGTTTGTTTCATTTCTAAATCCCTAGAATTATTTTCGTTTTCGCGCATTGAGCGAATcaa                                     >  2:343258/1‑76 (MQ=255)                                 taCCGTTGTGACGTTGTGTTTGTTTCATTTCTAAATCCCTAGAATTATTTTCGTTTTCGCGCATTGAGCGAATcaa                                     >  2:1326330/1‑76 (MQ=255)                                  aCCGTTGTGACGTTGTGTTTGTTTCATTTCTAAATCCCTAGAATTATTTTCGTTTTCGCGCATTGAGCGAATcaac                                    >  2:1943909/1‑76 (MQ=255)                                  aCCGTTGTGACGTTGTGTTTGTTTCATTTCTAAATCCCTAGAATTATTTTCGTTTTCGCGCATTGAGCGAATcaac                                    >  2:270482/1‑76 (MQ=255)                                   ccGTTGTGACGTTGTGTTTGTTTCATTTCTAAATCCCTAGAATTATTTTCGTTTTCGCGCATTGAGCGAATcaaca                                   <  1:356065/76‑1 (MQ=255)                                   ccGTTGTGACGTTGTGTTTGTTTCATTTCTAAATCCCTAGAATTATTTTCGTTTTCACGCATTGAGCGAATcaaca                                   <  1:31895/76‑1 (MQ=255)                                   ccGCTGTGACGTTGTGTTTGTTTCATTTCTAAATCCCTAGAATTATTTTCGTTTTCGCGCATTGAGCGAATcaaca                                   <  1:1690236/76‑1 (MQ=255)                                      ttGTGACGTTGTGTTTGTTTCATTTCTAAATCCCTAGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACaaaa                                >  1:1364252/1‑76 (MQ=255)                                        gtgACGTTGTGTTTGTTTCATTTCTAAATCCCTAGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGc                              >  1:55665/1‑76 (MQ=255)                                        gtgACGTTGTGTTTGTTTCATTTCTAAATCCCTAGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGc                              >  1:1265579/1‑76 (MQ=255)                                         tgACGTTGTGTTTGTTTCATTTCTAAATCCCTAGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGcc                             >  2:1662669/1‑76 (MQ=255)                                          gACGTTGTGTTTGTTTCATTTCTAAATCCCTAGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCa                            <  1:1344312/76‑1 (MQ=255)                                             gTTGTGTTTGTTTCATTTCTAAATCCCTAGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCg                         >  2:1733512/1‑76 (MQ=255)                                             gTTGTGTTTGTTTCATTTCTAAATCCCTAGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCg                         >  1:1049642/1‑76 (MQ=255)                                             gTTGTGTTTGTTTCATTTCTAAATCCCTAGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCg                         >  1:1324206/1‑76 (MQ=255)                                              ttGTGTTTGTTTCATTTCTAAATCCCTAGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGc                        >  2:145129/1‑76 (MQ=255)                                              ttGTGTTTGTTTCATTTCTAAATCCCTAGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGc                        >  2:1952231/1‑76 (MQ=255)                                               tgtgTTTGTTTCATTTCTAAATCCCTAGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCt                       <  1:79569/76‑1 (MQ=255)                                               tgtgTTTGTTTCATTTCTAAATCCCTAGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCt                       <  2:315538/76‑1 (MQ=255)                                                  gtttgtttCATTTCTAAATCCCTAGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTaaa                    <  1:85906/76‑1 (MQ=255)                                                  gtttgtttCATTTCTAAATCCCTAGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTaaa                    >  1:498660/1‑76 (MQ=255)                                                  gtttgtttCATTTCTAAATCCCTAGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTaaa                    <  1:1687153/76‑1 (MQ=255)                                                  gtttgtttCATTTCTAAATCCCTAGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTaaa                    <  1:1342104/76‑1 (MQ=255)                                                     tgtttCATTTCTAAATCCCTAGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAAccc                 >  1:769446/1‑76 (MQ=255)                                                      gtttCATTTCTAAATCCCTAGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCa                <  2:1371825/76‑1 (MQ=255)                                                       tttCATTTCTAAATCCCTAGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCAc               <  1:9710/76‑1 (MQ=255)                                                       tttCATTTCTAAATCCCTAGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCAc               <  2:207197/76‑1 (MQ=255)                                                        ttCATTTCTAAATCCCTAGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACg              >  1:458354/1‑76 (MQ=255)                                                           aTTTCTAAATCCCTAGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCt           <  1:1120944/76‑1 (MQ=255)                                                           aTTTCTAAATCCCTAGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCt           <  2:111675/76‑1 (MQ=255)                                                            tttCTAAATCCCTAGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCTa          <  1:686356/76‑1 (MQ=255)                                                            tttCTAAATCCCTAGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCTa          <  1:1340303/76‑1 (MQ=255)                                                             ttCTAAATCCCTAGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCTaa         <  2:154078/76‑1 (MQ=255)                                                              tCTAAATCCCTAGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCt           >  2:272541/1‑73 (MQ=255)                                                              tCTAAATCCCTAGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCt           <  1:272541/73‑1 (MQ=255)                                                               cTAAATCCCTAGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCTAAcc       >  1:294015/1‑76 (MQ=255)                                                               cTAAATCCCTAGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCTAAcc       <  2:74731/76‑1 (MQ=255)                                                               cTAAATCCCTAGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCTAAcc       <  1:2075963/76‑1 (MQ=255)                                                                 aaaTCCCTAGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCTAACCCg     >  1:1364540/1‑76 (MQ=255)                                                                  aaTCCCTAGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCTAACCCgg    >  2:1878408/1‑76 (MQ=255)                                                                   aTCCCTAGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCTAACCCGGc   >  2:1213884/1‑76 (MQ=255)                                                                   aTCCCTAGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCTAACCCGGc   >  2:903442/1‑76 (MQ=255)                                                                    tCCCTAGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCTAACCCGGCg  >  1:393648/1‑76 (MQ=255)                                                                         |                                                                       GACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTGACGTTGTGTTTGTTTCATTTCTAAATCCCTGGAATTATTTTCGTTTTCGCGCATTGAGCGAATCAACAAAAGCCATCGCTAAACCCACGGCTAACCCGGCG  >  REL606/3489147‑3489289

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑