Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	3,875,632	(T)7→8	intergenic (‑66/+287)	glmU ← / ← atpC	bifunctional N‑acetylglucosamine‑1‑phosphate uridyltransferase/glucosamine‑1‑phosphate acetyltransferase/F0F1 ATP synthase subunit epsilon

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	3,875,625	1	.	T	100.0%	206.0 / NA	51	intergenic (‑59/+294)	glmU/atpC	bifunctional N‑acetylglucosamine‑1‑phosphate uridyltransferase/glucosamine‑1‑phosphate acetyltransferase/F0F1 ATP synthase subunit epsilon
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base T (24/27);  total (24/27)

ATAGCATTATTCAACATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACA‑TTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTGACGAAAGCGCC  >  REL606/3875550‑3875695                                                                             |                                                                       aTAGCATTATTCAACATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACa                                                                         <  1:1327502/76‑1 (MQ=255)  tAGCATTATTCAACATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACA‑t                                                                       >  1:1697727/1‑76 (MQ=255)   aGCATTATTCAACATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACA‑tt                                                                      >  2:1318622/1‑76 (MQ=255)       ttattCAACATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACA‑tttttt                                                                  <  1:418095/76‑1 (MQ=255)         attCAACATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACA‑tttttttt                                                                >  2:1039745/1‑75 (MQ=255)          ttCAACATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACA‑ttttttttc                                                               >  2:351221/1‑74 (MQ=255)               cATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTTTTTTCATCGt                                                           <  2:94837/76‑1 (MQ=255)                aTACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTTTTTTCATCGTg                                                          <  2:1030243/76‑1 (MQ=255)                aTACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTTTTTTCATCGTg                                                          <  1:1115552/76‑1 (MQ=255)                  aCGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTTTTTTCATCGTGaa                                                        >  1:1193955/1‑76 (MQ=255)                  aCGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTTTTTTCATCGTGaa                                                        >  1:1503327/1‑76 (MQ=255)                   cgcgTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTTTTTTCATCGTGaaa                                                       >  2:258065/1‑76 (MQ=255)                   cgcgTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTTTTTTCATCGTGaaa                                                       <  1:1560072/76‑1 (MQ=255)                    gcgTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTTTTTTCATCGTGAAAt                                                      <  2:89768/76‑1 (MQ=255)                        ccTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTTTTTTCATCGTGAAATGGAc                                                  <  1:1501825/76‑1 (MQ=255)                         cTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTTTTTTCATCGTGAAATGGACa                                                 <  1:1630878/76‑1 (MQ=255)                         cTGACTATAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTTTTTTCATCGTGAAATGGACa                                                 >  1:891727/1‑76 (MQ=255)                          tGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTTTTTTCATCGTGAAATGGACag                                                >  1:621808/1‑76 (MQ=255)                             cTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTTTTTTCATCGTGAAATGGACAGAgg                                             >  1:1615519/1‑76 (MQ=255)                             cTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTTTTTTCATCGTGAAATGGACAGAgg                                             >  2:1475230/1‑76 (MQ=255)                              tGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTTTTTTCATCGTGAAATGGACAGAGGa                                            <  2:729881/76‑1 (MQ=255)                                tAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTTTTTTCATCGTGAAATGGACAGAGGATa                                          >  2:175994/1‑76 (MQ=255)                                 aaTTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTTTTTTCATCGTGAAATGGACAGAGGATaa                                         >  1:1530940/1‑76 (MQ=255)                                  aTTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTTTTTTCATCGTGAAATGGACGGAGGATaaa                                        <  1:396857/76‑1 (MQ=255)                                          aCGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCaa                                >  1:1287938/1‑76 (MQ=255)                                           cGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAAt                               <  1:577463/76‑1 (MQ=255)                                           cGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAAt                               <  1:577445/76‑1 (MQ=255)                                           cGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAAt                               <  2:56035/76‑1 (MQ=255)                                           cGAATTTAAACCGCTTCACCTTGAAAAAACTACATTTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAAt                               <  1:1569388/76‑1 (MQ=255)                                                  aaaCCGCTTCACCTTGAAAAAACTACATTTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGAt                        <  1:1953797/76‑1 (MQ=255)                                                  aaaCCGCTTCACCTTGAAAAAACTACATTTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGAt                        <  1:1702071/76‑1 (MQ=255)                                                   aaCCGCTTCACCTTGAAAAAACTACATTTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGAtt                       <  2:938771/76‑1 (MQ=255)                                                   aaCCGCTTCACCTTGAAAAAACTACATTTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGAtt                       <  2:1688075/76‑1 (MQ=255)                                                   aaCCGCTTCACCTTGAAAAAACTACATTTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGAtt                       >  1:1556653/1‑76 (MQ=255)                                                    aCCGCTTCACCTTGAAAAAACTACATTTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTa                      <  2:884657/76‑1 (MQ=255)                                                     ccGCTTCACCTTGAAAAAACTACATTTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTAt                     >  2:1889662/1‑76 (MQ=255)                                                     ccGCTTCACCTTGAAAAAACTACATTTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTAt                     <  1:1749678/76‑1 (MQ=255)                                                       gCTTCACCTTGAAAAAACTACATTTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATcc                   >  1:292373/1‑76 (MQ=255)                                                        cTTCACCTTGAAAAAACTACATTTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATccc                  >  1:1127540/1‑76 (MQ=255)                                                        cTTCACCTTGAAAAAACTACATTTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATccc                  <  1:1440701/76‑1 (MQ=255)                                                        cTTCACCTTGAAAAAACTACATTTTTTTTCATCGTGAAATGGACAGAAGATAAATTGTTCAATAACGGATTATccc                  >  1:508857/1‑76 (MQ=255)                                                         ttCACCTTGAAAAAACTACATTTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATcccc                 <  2:1031726/76‑1 (MQ=255)                                                         ttCACCTTGAAAAAACTACATTTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATcccc                 <  2:1828125/76‑1 (MQ=255)                                                           cACCTTGAAAAAACTACATTTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGc               >  1:44376/1‑76 (MQ=255)                                                           cACCTTGAAAAAACTACATTTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGc               <  1:1933168/76‑1 (MQ=255)                                                            aCCTTGAAAAAACTACATTTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCt              <  1:1692228/76‑1 (MQ=255)                                                             ccTTGAAAAAACTACATTTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTg             >  2:574584/1‑76 (MQ=255)                                                                tGAAAAAACTACATTTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTGACg          >  1:65118/1‑76 (MQ=255)                                                                tGAAAAAACTACATTTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTGACg          >  1:522366/1‑76 (MQ=255)                                                                tGAAAAAACTACATTTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTGACg          >  1:1432379/1‑76 (MQ=255)                                                                  aaaaaaCTACATTTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTGACGaa        >  2:1732645/1‑76 (MQ=255)                                                                   aaaaaCTACATTTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTGACGaaa       <  1:1190131/76‑1 (MQ=255)                                                                   aaaaaCTACATTTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTGACGaaa       <  1:1612763/76‑1 (MQ=255)                                                                    aaaaCTACATTTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTGACGAAAg      <  1:1442975/76‑1 (MQ=255)                                                                    aaaaCTACATTTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTGACGAAAg      >  1:291765/1‑76 (MQ=255)                                                                      aaCTACATTTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTGACGAAAgcg    >  2:398419/1‑76 (MQ=255)                                                                        cTACATTTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTGACGAAAGCGcc  >  1:960082/1‑76 (MQ=255)                                                                             |                                                                       ATAGCATTATTCAACATACGCGTCCTGACTGTAATTTGAGAACGAATTTAAACCGCTTCACCTTGAAAAAACTACA‑TTTTTTTCATCGTGAAATGGACAGAGGATAAATTGTTCAATAACGGATTATCCCCGCTGACGAAAGCGCC  >  REL606/3875550‑3875695

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑