| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 3,911,997 | (A)6→7 | intergenic (‑198/‑125) | yifB ← / → ilvL | predicted bifunctional enzyme and transcriptional regulator/ilvG operon leader peptide |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 3,911,991 | 1 | . | A | 97.7% | 174.1 / ‑2.7 | 44 | intergenic (‑192/‑131) | yifB/ilvL | predicted bifunctional enzyme and transcriptional regulator/ilvG operon leader peptide |
| Reads supporting (aligned to +/- strand): ref base . (0/1); new base A (20/23); total (20/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.50e-01 | |||||||||||
GACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTT‑AAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCAAAAAATATCTTGTACTATT > REL606/3911916‑3912062 | gACAAAGGATGAACTACGAGGAAGGGAACAACCTTCATACTGAAATTGAATTTTTTTCACTCACTATTTTAttttt > 1:1076001/1‑76 (MQ=255) aaaGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTT‑aaa > 2:78308/1‑76 (MQ=255) aaaGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTT‑aaa > 2:1738686/1‑76 (MQ=255) aaaGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTT‑aaa < 2:247174/76‑1 (MQ=255) aGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTT‑aaaaa < 2:1138617/76‑1 (MQ=255) gATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTT‑aaaaaaa < 2:1535404/76‑2 (MQ=255) aTGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTT‑aaaaaaac < 2:1125691/76‑3 (MQ=255) aCTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTTAAAAAAacaaca < 1:1333651/76‑1 (MQ=255) aCTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTTAAAAAAacaaca > 2:442919/1‑76 (MQ=255) gAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTTAAAAAAACAACAATTta > 1:355878/1‑76 (MQ=255) aGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTTAAAAAAACAACAATTtat < 1:820366/76‑1 (MQ=255) aGGAAGGGAACAACATTCAGACTGAAATTTAATTTTTTTCACTCACTATTTTATTTTTAAAAAAACAACAATTgat < 2:487352/76‑1 (MQ=255) aaGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTTAAAAAAACAACAATTTATAtt < 1:340692/76‑1 (MQ=255) acaacaTTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTTAAAAAAACAACAATTTATATTGAAatt > 2:238319/1‑76 (MQ=255) acaacaTTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTTAAAAAAACAACAATTTATATTGAAatt < 2:1814805/76‑1 (MQ=255) caacaTTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTTAAAAAAACAACAATTTATATTGAAatta < 2:76653/76‑1 (MQ=255) aacaTTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTTAAAAAAACAACAATTTATATTGAAattat < 2:1391311/76‑1 (MQ=255) acaTTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTTAAAAAAACAACAATTTATATTGAAattatt > 2:1172100/1‑76 (MQ=255) acaTTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTTAAAAAAACAACAATTTATATTGAAattatt < 2:1588914/76‑1 (MQ=255) aTTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTTAAAAAAACAACAATTTATATTGAAATTATTaa > 1:1730398/1‑76 (MQ=255) aTTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTTAAAAAAACAACAATTTATATTGAAATTATTaa > 1:1405010/1‑76 (MQ=255) ttCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTTAAAAAAACAACAATTTATATTGAAATTATTaaa > 2:740408/1‑76 (MQ=255) ttCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTTAAAAAAACAACAATTTATATTGAAATTATTaaa < 2:918810/76‑1 (MQ=255) tCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTTAAAAAAACAACAATTTATATTGAAATTATTAAAc > 1:831259/1‑76 (MQ=255) cATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTTAAAAAAACAACAATTTATATTGAAATTATTAAACg > 1:1189169/1‑76 (MQ=255) aTACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTTAAAAAAACAACAATTTATATTGAAATTATTAAACGc > 2:1414835/1‑76 (MQ=255) aCTGAAATTGAATTTTTTTCACTCACTATTTTATTTTTAAAAAAACAACAATTTATATTGAAATTATTAAACGcat > 2:892557/1‑76 (MQ=255) aCTGAAATTGAATTTTTTTCACTCACTATTTTATTTTTAAAAAAACAACAATTTATATTGAAATTATTAAACGcat > 2:1742329/1‑76 (MQ=255) tGAAATTGAATTTTTTTCACTCACTATTTTATTTTTAAAAAAACAACAATTTATATTGAAATTATTAAACGcatca > 2:1106339/1‑76 (MQ=255) tGAAATTGAATTTTTTTCACTCACTATTTTATTTTTAAAAAAACAACAATTTATATTGAAATTATTAAACGcatca < 2:1685528/76‑1 (MQ=255) gAAATTGAATTTTTTTCACTCACTATTTTATTTTTAAAAAAACAACAATTTATATTGAAATTATTAAACGcatcat < 1:1106476/76‑1 (MQ=255) aaaTTGAATTTTTTTCACTCACTATTTTATTTTTAAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATa < 1:50455/76‑1 (MQ=255) aaaTTGAATTTTTTTCACTCACTATTTTATTTTTAAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATa < 2:231299/76‑1 (MQ=255) aTTGAATTTTTTTCACTCACTATTTTATTTTTAAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATaaa < 2:994668/76‑1 (MQ=255) aTTGAATTTTTTTCACTCACTATTTTATTTTTAAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATaaa > 1:657872/1‑76 (MQ=255) ttGAATTTTTTTCACTCACTATTTTATTTTTAAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATaaaa < 1:1578208/76‑1 (MQ=255) ttGAATTTTTTTCACTCACTATTTTATTTTTAAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATaaaa < 1:644705/76‑1 (MQ=255) tGAATTTTTTTCACTCACTATTTTATTTTT‑AAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAAt < 1:1659183/76‑1 (MQ=255) gAATTTTTTTCACTCACTATTTTATTTTTAAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAAt > 1:1850033/1‑76 (MQ=255) aaTTTTTTTCACTCACTATTTTATTTTTAAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATc < 1:941947/76‑1 (MQ=255) ttttttCACTCACTATTTTATTTTTAAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGc > 1:24110/1‑76 (MQ=255) tcactcacTATTTTATTTTTAAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCaaaa < 1:841620/76‑1 (MQ=255) cactcactATTTTATTTTTAAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCaaaaa < 2:719614/76‑1 (MQ=255) tctcactATTTTATTTTTAAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCaaaaaa > 2:1913475/2‑76 (MQ=255) ctcactATTCTATTTTT‑AACAAACAACACTTTATATTGAAATTATTAAACGCATCCTAACAATCAGCCACCAAata > 2:914301/1‑76 (MQ=255) ctATTTTATTTTTAAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCAAAAAATATCt > 1:948976/1‑76 (MQ=255) tattttatttTTAAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCAAAAAATATCtt < 2:1773267/76‑1 (MQ=255) tattttatttTTAAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCAAAAAATATCtt > 2:1640022/1‑76 (MQ=255) tattttatttTTAAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCAAAAAATATCtt < 1:1464794/76‑1 (MQ=255) attttattttTAAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCAAAAAATATCTTg < 1:152443/76‑1 (MQ=255) ttttattttTAAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCAAAAAATATCTTGt < 1:1592461/76‑1 (MQ=255) tttattttTAAAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCAAAAAATATCTTGTa > 1:1267653/1‑76 (MQ=255) tttta‑AAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCAAAAAATATCTTGTACTAtt < 1:292058/76‑1 (MQ=255) tttta‑AAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCAAAAAATATCTTGTACTAtt < 2:905047/76‑1 (MQ=255) | GACAAAGGATGAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTT‑AAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCAAAAAATATCTTGTACTATT > REL606/3911916‑3912062 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |