| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 1,484,105 | 0 | A | G | 26.3% | 71.7 / 11.2 | 38 | G55G (GGA→GGG) | ydcU | predicted spermidine/putrescine transporter subunit |
| Reads supporting (aligned to +/- strand): ref base A (6/22); new base G (10/0); total (16/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.69e-05 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TACTGCTGCTTGGCCCACTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTGTTTAAT > REL606/1484032‑1484180 | tACTGCTGCTTGGCCCACTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGAtt < 2:1634179/76‑1 (MQ=255) ctgctgCTTGGCCCACTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGGtttt > 2:1807844/1‑76 (MQ=255) ctgctgCTTGGCCCACTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGAtttt < 1:1956846/76‑1 (MQ=255) ctgctgCTTGGCCCACTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGAtttt < 1:514416/76‑1 (MQ=255) ctgctTGGCCCACTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGGttttttt > 2:85546/1‑73 (MQ=255) gctTGGCCCACTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACt < 2:396543/76‑1 (MQ=255) ttGGCCCACTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGGGTTTTTTCttt > 2:1666911/1‑76 (MQ=255) ggCCCACTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTg < 2:754094/76‑1 (MQ=255) ggCCCACTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTg < 1:615676/76‑1 (MQ=255) gCCCACTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGa < 2:619863/76‑1 (MQ=255) cTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGGTTTTTTTATTTTTACGAtt > 2:1443945/1‑76 (MQ=255) tgGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGGTTTTTTACTTTTGACGATTTTACCa > 1:447838/1‑76 (MQ=255) tttGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGGTTTTTTTTTTTTTTCGATTTTACCATGt > 2:1948/1‑76 (MQ=255) tttGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGGTTTTTTACTTTTGACGATTTCACCATGt > 1:1804998/1‑76 (MQ=255) ttGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTc < 1:390677/76‑1 (MQ=255) tGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCg < 2:1339414/76‑1 (MQ=255) gCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGt < 2:1852659/76‑1 (MQ=255) aTTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTaa < 2:1408615/76‑1 (MQ=255) ttGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAAc < 2:217658/76‑1 (MQ=255) tGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGGTTTTTTACTTTTTACGATTTCACCATGTCGGGAACg > 2:244306/1‑76 (MQ=255) gTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGGTTTTTTTTTTTTTTCGATTTTACCATGTCGGTTACGc > 1:1026588/1‑76 (MQ=255) gTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGGTTTTTTTCTTTTTACGATTTTACCATGTCGGTAACGc > 1:992997/1‑76 (MQ=255) tCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGGTTTTATACTTTTTACGATTTCCCCCTGTCGGTAACGcc > 1:1372148/1‑76 (MQ=255) cTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGGTTTTTTACTTTTGACGATTTTACCATGTCGGTAACGCCg > 2:248848/1‑76 (MQ=255) tCGGCTCGCTGCTGACACTGTTATGGCAGGGGTTTTTTACTTTTTACGATTTCACCATGTCGGTAACGCCCGAACt > 2:1573667/1‑76 (MQ=255) gCTCGCTGCTGACACTGTTATGGCAGGGGTTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGAc > 1:1277475/1‑76 (MQ=255) gCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGAc > 2:1662580/1‑76 (MQ=255) cGCTGCTGACACTGTTATGGCAGGGATTTTTTACTTTTAACGATTTCACCATGTCGGTAACGCCGGAACTGACGCt > 2:697852/1‑76 (MQ=255) ctgctgACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCggg > 1:1945026/1‑76 (MQ=255) tgctgACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGc < 1:672304/76‑1 (MQ=255) tgctgACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGc < 2:146588/76‑1 (MQ=255) tgACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCgga > 2:900050/1‑74 (MQ=255) tgACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGaa > 2:1859676/1‑76 (MQ=255) cTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATca < 2:979772/76‑2 (MQ=255) tGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCg > 2:1841956/1‑76 (MQ=255) ttATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTg < 2:133330/76‑1 (MQ=255) aTGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTgcg < 1:1227462/76‑1 (MQ=255) ggATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTGTTTa < 2:1805636/76‑1 (MQ=255) ggATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTGTTTa < 2:1408518/76‑1 (MQ=255) gATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTGTTTaa < 1:262479/76‑1 (MQ=255) gATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTGTTTaa < 1:1789254/76‑1 (MQ=255) gATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTGTTTaa < 1:147784/76‑1 (MQ=255) aTTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTGTTTAAt > 2:1960/1‑76 (MQ=255) aTTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTGTTTAAt < 1:89556/76‑1 (MQ=255) | TACTGCTGCTTGGCCCACTAATGTGGTTTGGCATTGTCTATTTCGGCTCGCTGCTGACACTGTTATGGCAGGGATTTTATACTTTTGACGATTTCACCATGTCGGTAACGCCGGAACTGACGCTGGCGAATATCCGTGCGCTGTTTAAT > REL606/1484032‑1484180 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |