Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	645,855	A→G	L231S (TTA→TCA)	dacA ←	D‑alanyl‑D‑alanine carboxypeptidase (penicillin‑binding protein 5)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	645,855	0	A	G	100.0%	260.1 / NA	61	L231S (TTA→TCA)	dacA	D‑alanyl‑D‑alanine carboxypeptidase (penicillin‑binding protein 5)
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (27/34);  total (27/34)

AGCAACAAGGTTGTAACCTGCTTTGTCAGTGTGTCCGGTTTTGATGCCGTCGACATTCAGGCTGTTATCCCATAACAGGCCGTTACGGTTCAGCTGGCGAATACCGTTAAACGTAAATTCTTTTTCTTTATAGATCGAGTATTCA  >  REL606/645782‑645926                                                                          |                                                                        aGCAACAAGGTTGTAACCTGCTTTGTCAGTGTGTCCGGTTTTGATGCCGTCGACATTCAGGCTGTTATCCCATGAc                                                                       <  1:1654214/76‑1 (MQ=255) aGCAACAAGGTTGTAACCTGCTTTGTCAGTGTGTCCGGTTTTGATGCCGTCGACATTCAGGCTGTTATCCCATGAc                                                                       <  2:1399159/76‑1 (MQ=255)  gCAACAAGGTTGTAACCTGCTTTGTCAGTGTGTCCGGTTTTGATGCCGTCGACATTCAGGCTGTTATCCCATGACa                                                                      <  1:76923/76‑1 (MQ=255)    aacaaGGTTGTAACCTGCTTTGTCAGTGTGTCCGGTTTTGATGCCGTCGACATTCAGGCTGTTATCCCATGACAgg                                                                    >  1:47691/1‑76 (MQ=255)    aacaaGGTTGTAACCTGCTTTGTCAGTGTGTCCGGTTTTGATGCCGTCGACATTCAGGCTGTTATCCCATGACAgg                                                                    >  1:1325132/1‑76 (MQ=255)    aacaaGGTTGTAACCTGCTTTGTCAGTGTGTCCGGTTTTGATGCCGTCGACATTCAGGCTGTTATCCCATGACAgg                                                                    <  1:1774375/76‑1 (MQ=255)      caaGGTTGTAACCTGCTTTGTCAGTGTGTCCGGTTTTGATGCCGTCGACATTCAGGCTGTTATCCCATGACAGGcc                                                                  <  1:402655/76‑1 (MQ=255)       aaGGTTGTAACCTGCTTTGTCAGTGTGTCCGGTTTTGATGCCGTCGACATTCAGGCTGTTATCCCATGACAGGCCg                                                                 >  2:813230/1‑76 (MQ=255)       aaGGTTGTAACCTGCTTTGTCAGTGTGTCCGGTTTTGATGCCGTCGACATTCAGGCTGTTATCCCATGACAGGCCg                                                                 >  2:325242/1‑76 (MQ=255)        aGGTTGTAACCTGCTTTGTCAGTGTGTCCGGTTTTGATGCCGTCGACATTCAGGCTGTTATCCCATGACAGGCCGt                                                                >  2:1310223/1‑76 (MQ=255)        aGGGTGTAACCTGCTTTGTCAGTGTGTCCGGTTTTGATGCCGTCGACATTCAGGCTGTTATCCCATGACAGGCCGt                                                                >  2:1310239/1‑76 (MQ=255)            tGTAACCTGCTTTGTCAGTGTGTCCGGTTTTGATGCCGTCGACATTCAGGCTGTTATCCCATGACAGGCCGTTACg                                                            >  1:1103505/1‑76 (MQ=255)            tGTAACCTGCTTTGTCAGTGTGTCCGGTTTTGATGCCGTCGACATTCAGGCTGTTATCCCATGACAGGCCGTTACg                                                            >  1:65928/1‑76 (MQ=255)              tAACCTGCTTTGTCAGTGTGTCCGGTTTTGATGCCGTCGACATTCAGGCTGTTATCCCATGACAGGCCGTTACGGt                                                          >  2:1267527/1‑76 (MQ=255)              tAACCTGCTTTGTCAGTGTGTCCGGTTTTGATGCCGTCGACATTCAGGCTGTTATCCCATGACAGGCCGTTACGGt                                                          >  1:772068/1‑76 (MQ=255)                cgatGCTTTGTCAGTGTGTCCGGTTTTGATGCCGTCGACATTCAGGCTGTTATCCCATGACAGGCCGTTACGGTTc                                                        >  2:851893/4‑76 (MQ=255)                   tGCTTTGTCAGTGTGTCCGGTTTTGATGCCGTCGACATTCAGGCTGTTATCCCATGACAGGCCGTTACGGTTCAGc                                                     >  2:172958/1‑76 (MQ=255)                    gCTTTGTCAGTGTGTCCGGTTTTGATGCCGTCGACATTCAGGCTGTTATCCCATGACAGGCCGTTACGGTTCAGCt                                                    >  2:1670277/1‑76 (MQ=255)                        tGTCAGTGTGTCCGGTTTTGATGCCGTCGACATTCAGGCTGTTATCCCATGACAGGCCGTTACGGTTCAGCTGGCg                                                <  1:291596/76‑1 (MQ=255)                        tGTCAGTGTGTCCGGTTTTGATGCCGTCGACATTCAGGCTGTTATCCCATGACAGGCCGTTACGGTTCAGCTGGCg                                                >  1:1605255/1‑76 (MQ=255)                           cAGTGTGTCCGGTTTTGATGCCGTCGACATTCAGGCTGTTATCCCATGACAGGCCGTTACGGTTCAGCTGGCGAAt                                             <  2:85137/76‑1 (MQ=255)                           cAGTGTGTCCGGTTTTGATGCCGTCGACATTCAGGCTGTTATCCCATGACAGGCCGTTACGGTTCAGCTGGCGAAt                                             >  1:684367/1‑76 (MQ=255)                            aGTGTGTCCGGTTTTGATGCCGTCGACATTCAGGCTGTTATCCCATGACAGGCCGTTACGGTTCAGCTGGCGAATa                                            <  2:1342106/76‑1 (MQ=255)                            aGTGTGTCCGGTTTTGATGCCGTCGACATTCAGGCTGTTATCCCATGACAGGCCGTTACGGTTCAGCTGGCGAATa                                            >  2:1105222/1‑76 (MQ=255)                            aGTGGGGCCGGTTTTGATGCCGTCGACATTCAGGCTGTTATCCCATGACAGGCCGTTACGGTTCAGCTGGCGAATa                                            <  2:950829/76‑1 (MQ=255)                               gtgtCCGGTTTTGATGCCGTCGACATTCAGGCTGTTATCCCATGACAGGCCGTTACGGTTCAGCTGGCGAATACCg                                         <  2:1932140/76‑1 (MQ=255)                                 gtCCGGTTTTGATGCCGTCGACATTCAGGCTGTTATCCCATGACAGGCCGTTACGGTTCAGCTGGCGAATACCGtt                                       >  1:820919/1‑76 (MQ=255)                                 gtCCGGTTTTGATGCCGTCGACATTCAGGCTGTTATCCCATGACAGGCCGTTACGGTTCAGCTGGCGAATACCGtt                                       >  2:1433753/1‑76 (MQ=255)                                 gtCCGGTTTTGATGCCGTCGACATTCAGGCTGTTATCCCATGACAGGCCGTTACGGTTCAGCTGGCGAATACCGtt                                       <  1:1469330/76‑1 (MQ=255)                                  tCCGGTTTTGATGCCGTCGACATTCAGGCTGTTATCCCATGACAGGCCGTTACGGTTCAGCTGGCGAATACCGTTa                                      <  2:1299482/76‑1 (MQ=255)                                  tCCGGTTTTGATGCCGTCGACATTCAGGCTGTTATCCCATGACAGGCCGTTACGGTTCAGCTGGCGAATACCGTTa                                      >  1:396443/1‑76 (MQ=255)                                  tCCGGTTTTGATGCCGTCGACATTCAGGCTGTTATCCCATGACAGGCCGTTACGGTTCAGCTGGCGAATACCGTTa                                      <  2:1324608/76‑1 (MQ=255)                                  tCCGGTTTTGATGCCGTCGACATTCAGGCTGTTATCCCATGACAGGCCGTTACGGTTCAGCTGGCGAATACCGTTa                                      <  2:1920761/76‑1 (MQ=255)                                  tCCGGTTTTGATGCCGTCGACATTCAGGCTGTTATCCCATGACAGGCCGTTACGGTTCAGCTGGCGAATACCGTTa                                      <  2:1700805/76‑1 (MQ=255)                                  tCCGGTTTTGATGCCGTCGACATGCAGGCTGTTATCCCATGACAGGCCGTTACGGTTCAGCTGGCGAATACCGTTa                                      <  2:1299466/76‑1 (MQ=255)                                   ccGGTTTTGATGCCGTCGACATTCAGGCTGTTATCCCATGACAGGCCGTTACGGTTCAGCTGGCGAATACCGTTaa                                     <  2:618432/76‑1 (MQ=255)                                   ccGGTTTTGATGCCGTCGACATTCAGGCTGTTATCCCATGACAGGCCGTTACGGTTCAGCTGGCGAATACCGTTaa                                     <  2:517767/76‑1 (MQ=255)                                   ccGGTTTTGATGCCGTCGACATTCAGGCTGTTATCCCATGACAGGCCGTTACGGTTCAGCTGGCGAATACCGTTaa                                     <  2:417879/76‑1 (MQ=255)                                   ccGGTTTTGATGCCGTCGACATTCAGGCTGTTATCCCATGACAGGCCGTTACGGTTCAGCTGGCGAATACCGTTaa                                     <  2:1392701/76‑1 (MQ=255)                                    cGGTTTTGATGCCGTCGACATTCAGGCTGTTATCCCATGACAGGCCGTTACGGTTCAGCTGGCGAATACCGTTaaa                                    <  1:529526/76‑1 (MQ=255)                                      gTTTTGATGCCGTCGACATTCAGGCTGTTATCCCATGACAGGCCGTTACGGTTCAGCTGGCGAATACCGTTAAACg                                  >  1:1213453/1‑76 (MQ=255)                                       ttttGATGCCGTCGACATTCAGGCTGTTATCCCATGACAGGCCGTTACGGTTCAGCTGGCGAATACCGTTAAACGt                                 >  2:649071/1‑76 (MQ=255)                                          tGATGCCGTCGACATTCAGGCTGTTATCCCATGACAGGCCGTTACGGTTCAGCTGGCGAATACCGTTAAACGTaaa                              >  1:1519312/1‑76 (MQ=255)                                          tGATGCCGTCGACATTCAGGCTGTTATCCCATGACAGGCCGTTACGGTTCAGCTGGCGAATACCGTTAAACGTaaa                              <  1:294045/76‑1 (MQ=255)                                             tGCCGTCGACATTCAGGCTGTTATCCCATGACAGGCCGTTACGGTTCAGCTGGCGAATACCGTTAAACGTAAAttc                           <  2:47691/76‑1 (MQ=255)                                             tGCCGTCGACATTCAGGCTGTTATCCCATGACAGGCCGTTACGGTTCAGCTGGCGAATACCGTTAAACGTAAAttc                           <  2:1325132/76‑1 (MQ=255)                                              gCCGTCGACATTCAGGCTGTTATCCCATGACAGGCCGTTACGGTTCAGCTGGCGAATACCGTTAAACGTAAAttct                          <  1:1133213/76‑1 (MQ=255)                                              gCCGTCGACATTCAGGCTGTTATCCCATGACAGGCCGTTACGGTTCAGCTGGCGAATACCGTTAAACGTAAAttct                          <  2:820919/76‑1 (MQ=255)                                               ccGTCGACATTCAGGCTGTTATCCCATGACAGGCCGTTACGGTTCAGCTGGCGAATACCGTTAAACGTAAAttctt                         >  2:757031/1‑76 (MQ=255)                                                  tCGACATTCAGGCTGTTATCCCATGACAGGCCGTTACGGTTCAGCTGGCGAATACCGTTAAACGTAAAttcttttt                      <  2:32007/76‑1 (MQ=255)                                                  tCGACATTCAGGCTGTTATCCCATGACAGGCCGTTACGGTTCAGCTGGCGAATACCGTTAAACGTAAAttcttttt                      <  2:785000/76‑1 (MQ=255)                                                    gACATTCAGGCTGTTATCCCATGACAGGCCGTTACGGTTCAGCTGGCGAATACCGTTAAATGTAAAttcttttttt                    >  1:110153/1‑74 (MQ=255)                                                    gACATTCAGGCTGTTATCCCATGACAGGCCGTTACGGTTCAGCTGGCGAATACCGTTAAACGCAAAttctttttct                    >  2:133805/1‑76 (MQ=255)                                                       aTTCAGGCTGTTATCCCATGACAGGCCGTTACGGTTCAGCTGGCGAATACCGTTAAACGTAAATTCTTTTTCTTta                 >  1:1377841/1‑76 (MQ=255)                                                       aTTCAGGCTGTTATCCCATGACAGGCCGTTACGGTTCAGCTGGCGAATACCGTTAAACGTAAATTCTTTTTCTTta                 <  2:794000/76‑1 (MQ=255)                                                            ggCTGTTATCCCATGACAGGCCGTTACGGTTCAGCTGGCGAATACCGTTAAACGTAAATTCTTTTTCTTTATAGAt            <  2:1666136/76‑1 (MQ=255)                                                               tGTTATCCCATGACAGGCCGTTACGGTTCAGCTGGCGAATACCGTTAAACGTAAATTCTTTTTCTTTATAGATCGa         >  1:364548/1‑76 (MQ=255)                                                                 ttATCCCATGACAGGCCGTTACGGTTCAGCTGGCGAATACCGTTAAACGTAAATTCTTTTTCTTTATAGATCGAGt       <  1:264427/76‑1 (MQ=255)                                                                   aTCCCATGACAGGCCGTTACGGTTCAGCTGGCGAATACCGTTAAACGTAAATTCTTTTTCTTTATAGATCGAGTat     <  2:1595787/76‑1 (MQ=255)                                                                    tCCCATGACAGGCCGTTACGGTTCAGCTGGCGAATACCGTTAAACGTAAATTCTTTTTCTTTATAGATCGAGTatt    <  2:1951950/76‑1 (MQ=255)                                                                      ccATGACAGGCCGTTACGGTTCAGCTGGCGAATACCGTTAAACGTAAATTCTTTTTCTTTATAGATCGAGTattca  >  2:664831/1‑76 (MQ=255)                                                                      ccATGACAGGCCGTTACGGTTCAGCTGGCGAATACCGTTAAACGTAAATTCTTTTTCTTTATAGATCGAGTattca  <  1:1433753/76‑1 (MQ=255)                                                                          |                                                                        AGCAACAAGGTTGTAACCTGCTTTGTCAGTGTGTCCGGTTTTGATGCCGTCGACATTCAGGCTGTTATCCCATAACAGGCCGTTACGGTTCAGCTGGCGAATACCGTTAAACGTAAATTCTTTTTCTTTATAGATCGAGTATTCA  >  REL606/645782‑645926

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑