| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 3,881,536 | T→G | D7A (GAT→GCT) | atpE ← | F0F1 ATP synthase subunit C |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 3,881,536 | 0 | T | G | 94.9% | 224.4 / 0.9 | 59 | D7A (GAT→GCT) | atpE | F0F1 ATP synthase subunit C |
| Reads supporting (aligned to +/- strand): ref base T (3/0); new base G (26/30); total (29/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.12e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.90e-01 | |||||||||||
GAGGATGCCGATACCGATCGCAGCACCGATTGCCGCCAGACCCATCATCACAGCGGCAGCCATGTACAGCAGATCCATATTCAGGTTTTCCATGACAGTCTCCAGTTTGTTTCAGTTAAAACGTAGTAGTGTTGGTAAAT > REL606/3881463‑3881602 | gAGGATGCCGATACCGATCGCAGCACCGATTGCCGCCAGACCCATCATCACAGCGGCAGCCATGTACAGCAGAGcc > 1:96831/1‑76 (MQ=255) aGGATGCCGATACCGATCGCAGCACCGATTGCCGCCAGACCCATCATCACAGCGGCAGCCATGTACAGCAGAGCCa > 1:1849434/1‑76 (MQ=255) aGGATGCCGATACCGATCGCAGCACCGATTGCCGCCAGACCCATCATCACAGCGGCAGCCATGTACAGCAGAGCCa < 1:850287/76‑1 (MQ=255) aGGATGCCGATACCGATCGCAGCACCGATTGCCGCCAGACCCATCATCACAGCGGCAGCCATGTACAGCAGAGCCa < 1:554595/76‑1 (MQ=255) ggATGCCGATACCGATCGCAGCACCGATTGCCGCCAGACCCATCATCACAGCGGCAGCCATGTACAGCAGAGCCat < 1:1681209/76‑1 (MQ=255) aTGCCGATCCCGATCGCAGCCCCGATTGCCGCCAGACCCATCATCACAGCGGCAGCCATGTACAGCAGAGCCatat < 2:879887/76‑1 (MQ=255) aTGCCGATACCGATCGCAGCACCGATTGCCGCCAGACCCATCATCACAGCGGCAGCCATGTACAGCAGAGCCatat < 2:563263/76‑1 (MQ=255) tGCCGATACCGATCGCAGCACCGATTGCCGCCAGACCCATCATCACAGCGGCAGCCATGTACAGCAGAGCCATAtt < 2:1502345/76‑1 (MQ=255) tGCCGATACCGATCGCAGCACCGATTGCCGCCAGACCCATCATCACAGCGGCAGCCATGTACAGCAGAGCCATAtt > 2:674034/1‑76 (MQ=255) ccGATACCGATCGCAGCACCGATTGCCGCCAGACCCATCATCACAGCGGCAGCCATGTACAGCAGAGCCATATTCa > 2:1342449/1‑76 (MQ=255) aTACCGATCGCAGCACCGATTGCCGCCAGACCCATCATCACAGCGGCAGCCATGTACAGCAGAGCCATATTCAGGt > 2:1727231/1‑76 (MQ=255) aTACCGATCGCAGCACCGATTGCCGCCAGACCCATCATCACAGCGGCAGCCATGTACAGCAGAGCCATATTCAGGt > 2:543373/1‑76 (MQ=255) tACCGATCGCAGCACCGATTGCCGCCAGACCCATCATCACAGCGGCAGCCATGTACAGCAGAGCCATATTCAGGtt < 1:1075733/76‑1 (MQ=255) aCCGATCGCAGCACCGATTGCCGCCAGACCCATCATCACAGCGGCAGCCATGTACAGCAGAGCCATATTCAGGttt > 2:1662094/1‑76 (MQ=255) aCCGATCGCAGCACCGATTGCCGCCAGACCCATCATCACAGCGGCAGCCATGTACAGCAGAGCCATATTCAGGttt > 2:218404/1‑76 (MQ=255) cGATCGCAGCACCGATTGCCGCCAGACCCATCATCACAGCGGCAGCCATGTACAGCAGAGCCATATTCAGGTTTTc < 2:181549/76‑1 (MQ=255) gATCGCAGCACCGATTGCCGCCAGACCCATCATCACAGCGGCAGCCATGTACAGCAGAGCCATATTCAGGTTTTcc < 1:1542313/76‑1 (MQ=255) gATCGCAGCACCGATTGCCGCCAGACCCATCATCACAGCGGCAGCCATGTACAGCAGAGCCATATTCAGGTTTTcc > 1:698669/1‑76 (MQ=255) aTCGCAGCACCGATTGCCGCCAGACCCATCATCACAGCGGCAGCCATGTACAGCAGAGCCATATTCAGGTTTTCCa < 1:250867/76‑1 (MQ=255) cGCAGCACCGATTGCCGCCAGACCCATCATCACAGCGGCAGCCATGTACAGCAGAGCCATATTCAGGTTTTCCATg > 2:1907446/1‑76 (MQ=255) cagcaCCGATTGCCGCCAGACCCATCATCACAGCGGCAGCCATGTACAGCAGAGCCATATTCAGGTTTTCCATGAc < 1:1612545/76‑1 (MQ=255) cagcaCCGATTGCCGCCAGACCCATCATCACAGCGGCAGCCATGTACAGCAGAGCCATATTCAGGTTTTCCATGAc > 2:1191093/1‑76 (MQ=255) agcaCCGATTGCCGCCAGACCCATCATCACAGCGGCAGCCATGTACAGCAGAGCCATATTCAGGTTTTCCATGACa > 2:1560897/1‑76 (MQ=255) agcaCCGATTGCCGCCAGACCCATCATCACAGCGGCAGCCATGTACAGCAGAGCCATATTCAGGTTTTCCATGACa > 2:555216/1‑76 (MQ=255) gcaCCGATTGCCGCCAGGCCCATCATCACAGCGGCAGCCATGTACAGCAGAGCCATATTCAGGTTTTCCATGACAg < 2:759293/76‑1 (MQ=255) gcaCCGATTGCCGCCAGACCCATCATCACAGCGGCAGCCATGTACAGCAGAGCCATATTCAGGTTTTCCATGACAg < 2:414845/76‑1 (MQ=255) gcaCCGATTGCCGCCAGACCCATCATCACAGCGGCAGCCATGTACAGCAGAGCCATATTCAGGTTTTCCATGACAg < 2:759279/76‑1 (MQ=255) aCCGATTGCCGCCAGACCCATCATCACAGCGGCAGCCATGTACAGCAGAGCCATATTCAGGTTTTCCATGACAGtc < 2:620383/76‑1 (MQ=255) cGATTGCCGCCAGACCCATCATCACAGCGGCAGCCATGTACAGCAGAGCCATATTCAGGTTTTCCATGACAGtctc > 2:78228/1‑76 (MQ=255) gATTGCCGCCAGACCCATCATCACAGCGGCAGCCATGTACAGCAGAGCCATATTCAGGTTTTCCATGACAGTCTcc < 1:283319/76‑1 (MQ=255) aTTGCCGCCAGACCCATCATCACAGCGGCAGCCATGTACAGCAGAGCCATATTCAGGTTTTCCATGACAGTCTCCa < 2:491999/76‑1 (MQ=255) ccgccAGACCCATCATCACAGCGGCAGCCATGTACAGCAGAGCCATATTCAGGTTTTCCATGTCAGTCTCCCgttt > 2:612479/1‑76 (MQ=255) ccgccAGACCCATCATCACAGCGGCAGCCATGTACAGCAGAGCCATATTCAGGTTTTCCATGACAGTCTCCAgttt < 2:723293/76‑1 (MQ=255) aGACCCATCATCACAGCGGCAGCCATGTACAGCAGAGCCATATTCAGGTTTTCCATGACAGTCTCCAGTTTGTTTc > 1:1936343/1‑76 (MQ=255) aCCCATCATCACAGCGGCAGCCATGTACAGCAGAGCCATATTCAGGTTTTCCATGACAGTCTCCAGTTTGTTTCAg > 2:108321/1‑76 (MQ=255) aCCCATCATCACAGCGGCAGCCATGTACAGCAGAGCCATATTCAGGTTTTCCATGACAGTCTCCAGTTTGTTTCAg < 1:13739/76‑1 (MQ=255) atcatcACAGCGGCAGCCATGTACAGCAGAGCCATATTCAGGTTTTCCATGACAGTCTCCAGTTTGTTTCAGTTaa > 1:1721380/1‑76 (MQ=255) atcatcACAGCGGCAGCCATGTACAGCAGAGCCATATTCAGGTTTTCCATGACAGTCTCCAGTTTGTTTCAGTTaa > 1:562144/1‑76 (MQ=255) tcatcaCAGCGGCAGCCATGTACAGCAGAGCCATATTCAGGTTTTCCATGACAGTCTCCAGTTTGTTTCAGTTaaa < 1:489541/76‑1 (MQ=255) catcaCAGCGGCAGCCATGTACAGCAGAGCCATATTCAGGTTTTCCATGACAGTCTCCAGTTTGTTTCAGTTaaaa < 2:1210526/76‑1 (MQ=255) atcaCAGCGGCAGCCATGTACAGCAGAGCCATATTCAGGTTTTCCATGACAGTCTCCAGTTTGTTTCAGTTAAAAc < 2:146434/76‑1 (MQ=255) cacaGCGGCAGCCATGTACAGCAGAGCCATATTCAGGTTTTCCATGACAGTCTCCAGTTTGTTTCAGTTAAAACgt < 2:1176293/76‑1 (MQ=255) aGCGGCAGCCATGTACAGCAGAGCCATATTCAGGTTTTCCATGACAGTCTCCAGTTTGTTTCAGTTAAAACgtagt > 2:1509171/1‑76 (MQ=255) aGCGGCAGCCATGTACAGCAGAGCCATATTCAGGTTTTCCATGACAGTCTCCAGTTTGTTTCAGTTAAAACgtagt < 1:978496/76‑1 (MQ=255) aGCGGCAGCCATGTACAGCAGAGCCATATTCAGGTTTTCCATGACAGTCTCCAGTTTGTTTCAGTTAAAACgtagt > 1:29919/1‑76 (MQ=255) aGCGGCAGCCATGTACAGCAGAGCCATATTCAGGTTTTCCATGACAGTCTCCAGTTTGTTTCAGTTAAAACgtagt < 1:218404/76‑1 (MQ=255) ggCAGCCATGTACAGCAGATCCATATTCAGGTTTTCCATGACAGTCTCCAGTTTGTTTCAGTTAAAACGTagtagt > 1:735179/1‑76 (MQ=255) ggCAGCCATGTACAGCAGAGCCATATTCAGGTTTTCCATGACAGTCTCCAGTTTGTTTCAGTTAAAACGTagtagt > 1:1269274/1‑76 (MQ=255) gCAGCCATGTACAGCAGAGCCATATTCAGGTTTTCCATGACAGTCTCCAGTTTGTTTCAGTTAAAACGTAGTAgtg > 1:1036839/1‑76 (MQ=255) cAGCCATGTACAGCAGATCCATATTCAGGTTTTCCATGACAGTCTCCAGTTTGTTTCAGTTAAAACGTAGTAgtgt > 1:1611440/1‑76 (MQ=255) cAGCCATGTACAGCAGAGCCATATTCAGGTTTTCCATGACAGTCTCCAGTTTGTTTCAGTTAAAACGTAGTAgtgt > 2:825187/1‑76 (MQ=255) aGCCATGTACAGCAGATCCATATTCAGGTTTTCCATGACAGTCTCCAGTTTGTTTCAGTTAAAACGTAGTAGTGtt > 1:1574031/1‑76 (MQ=255) aGCCATGTACAGCAGAGCCATATTCAGGTTTTCCATGACAGTCTCCAGTTTGTTTCAGTTAAAACGTAGTAGTGtt < 2:1211940/76‑1 (MQ=255) cATGTACAGCAGAGCCATATTCAGGTTTTCCATGACAGTCTCCAGTTTGTTTCAGTTAAAACGTAGTAGTGTTGGt < 2:218380/76‑1 (MQ=255) aTGTACAGCAGAGCCATATTCAGGTTTTCCATGACAGTCTCCAGTTTGTTTCAGTTAAAACGTAGTAGTGTTGGTa < 2:1919488/76‑1 (MQ=255) aTGTACAGCAGAGCCATATTCAGGTTTTCCATGACAGTCTCCAGTTTGTTTCAGTTAAAACGTAGTAGTGTTGGTa > 1:828433/1‑76 (MQ=255) gTACAGCAGAGCCATATTCAGGTTTTCCATGACAGTCTCCAGTTTGTTTCAGTTAAAACGTAGTAGTGTTGGTaaa < 2:135568/76‑1 (MQ=255) gTACAGCAGAGCCATATTCAGGTTTTCCATGACAGTCTCCAGTTTGTTTCAGTTAAAACGTAGTAGTGTTGGTaaa < 2:90613/76‑1 (MQ=255) tACAGCAGAGCCATATTCAGGTTTTCCATGACAGTCTCCAGTTTGTTTCAGTTAAAACGTAGTAGTGTTGGTAAAt > 1:442730/1‑76 (MQ=255) | GAGGATGCCGATACCGATCGCAGCACCGATTGCCGCCAGACCCATCATCACAGCGGCAGCCATGTACAGCAGATCCATATTCAGGTTTTCCATGACAGTCTCCAGTTTGTTTCAGTTAAAACGTAGTAGTGTTGGTAAAT > REL606/3881463‑3881602 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |