Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	4,442,003	C→A	A265E (GCG→GAG)	pmbA →	predicted peptidase required for the maturation and secretion of the antibiotic peptide MccB17

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	4,442,003	0	C	A	100.0%	282.8 / NA	71	A265E (GCG→GAG)	pmbA	predicted peptidase required for the maturation and secretion of the antibiotic peptide MccB17
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (35/36);  total (35/36)

ACCATGAAAGCGCCAGTCATTTTTGCCAATGAAGTGGCAACCGGGCTTTTTGGCCATCTGGTGGGGGCGATAGCGGGTGGCTCGGTTTACCGTAAATCTACCTTTCTGCTGGATTCGCTGGGTAAACAAATTCTGCCGGACTGGCT  >  REL606/4441930‑4442075                                                                          |                                                                         accaTGAAAGCGCCAGTCATTTTTGCCAATGAAGTGGCAACCGGGCTTTTTGGCCATCTGGTGGGGGCGATAGAgg                                                                        >  2:1219306/1‑76 (MQ=255)  ccaTGAAAGCGCCAGTCATTTTTGCCAATGAAGTGGCAACCGGGCTTTTTGGCCATCTGGTGGGGGCGATAGAggg                                                                       >  2:536696/1‑76 (MQ=255)  ccaTGAAAGCGCCAGTCATTTTTGCCAATGAAGTGGCAACCGGGCTTTTTGGCCATCTGGTGGGGGCGATAGAggg                                                                       <  2:542743/76‑1 (MQ=255)     tGAAAGCGCCAGTCATTTTTGCCAATGAAGTGGCAACCGGGCTTTTTGGCCATCTGGTGGGGGCGATAGAGGGTgg                                                                    <  2:1879170/76‑1 (MQ=255)     tGAAAGCGCCAGTCATTTTTGCCAATGAAGTGGCAACCGGGCTTTTTGGCCATCTGGTGGGGGCGATAGAGGGTgg                                                                    >  1:228843/1‑76 (MQ=255)       aaaGCGCCAGTCATTTTTGCCAATGAAGTGGCAACCGGGCTTTTTGGCCATCTGGTGGGGGCTATAGAGGGTGGCt                                                                  <  2:1824574/76‑1 (MQ=255)       aaaGCGCCAGTCATTTTTGCCAATGAAGTGGCAACCGGGCTTTTTGGCCATCTGGTGGGGGCGATAGAGGGTGGCt                                                                  <  2:813434/76‑1 (MQ=255)       aaaGCGCCAGTCATTTTTGCCAATGAAGTGGCAACCGGGCTTTTTGGCCATCTGGTGGGGGCGATAGAGGGTGGCt                                                                  <  2:204053/76‑1 (MQ=255)       aaaGCGCCAGTCATTTTTGCCAATGAAGTGGCAACCGGGCTTTTTGGCCATCTGGTGGGGGCGATAGAGGGTGGCt                                                                  >  2:790133/1‑76 (MQ=255)        aaGCGCCAGTCATTTTTGCCAATGAAGTGGCAACCGGGCTTTTTGGCCATCTGGTGGGGGCGATAGAGGGTGGCTc                                                                 >  2:447231/1‑76 (MQ=255)          gcgcCAGTCATTTTTGCCAATGAAGTGGCAACCGGGCTTTTTGGCCATCTGGTGGGGGCGATAGAGGGTGGCTCgg                                                               <  1:1606010/76‑1 (MQ=255)          gcgcCAGTCATTTTTGCCAATGAAGTGGCAACCGGGCTTTTTGGCCATCTGGTGGGGGCGATAGAGGGTGGCTCgg                                                               <  1:28430/76‑1 (MQ=255)             ccAGTCATTTTTGCCAATGAAGTGGCAACCGGGCTTTTTGGCCATCTGGTGGGGGCGATAGAGGGTGGCTCGGttt                                                            <  2:1736843/76‑1 (MQ=255)             ccAGTCATTTTTGCCAATGAAGTGGCAACCGGGCTTTTTGGCCATCTGGTGGGGGCGATAGAGGGTGGCTCGGttt                                                            <  1:585595/76‑1 (MQ=255)              cAGTCATTTTTGCCAATGAAGTGGCAACCGGGCTTTTTGGCCATCTGGTGGGGGCGATAGAGGGTGGCTCGGTTTa                                                           <  1:971318/76‑1 (MQ=255)              cAGTCATTTTTGCCAATGAAGTGGCAACCGGGCTTTTTGGCCATCTGGTGGGGGCGATAGAGGGTGGCTCGGTTTa                                                           <  1:800073/76‑1 (MQ=255)              cAGTCATTTTTGCCAATGAAGTGGCAACCGGGCTTTTTGGCCATCTGGTGGGGGCGATAGAGGGTGGCTCGGTTTa                                                           <  1:1592939/76‑1 (MQ=255)                 tCATTTTTGCCAATGAAGTGGCAACCGGGCTTTTTGGCCATCTGGTGGGGGCGATAGAGGGTGGCTCGGTTTACCg                                                        <  2:713241/76‑1 (MQ=255)                  cATTTTTGCCAATGAAGTGGCAACCGGGCTTTTTGGCCATCTGGTGGGGGCGATAGAGGGTGGCTCGGTTTACCGt                                                       >  2:1607251/1‑76 (MQ=255)                  cATTTTTGCCAATGAAGTGGCAACCGGGCTTTTTGGCCATCTGGTGGGGGCGATAGAGGGTGGCTCGGTTTACCGt                                                       >  1:1033289/1‑76 (MQ=255)                   aTTTTTGCCAATGAAGTGGCAACCGGGCTTTTTGGCCATCTGGTGGGGGCGATAGAGGGTGGCTCGGTTTACCGTa                                                      >  2:743469/1‑76 (MQ=255)                   aTTTTTGCCAATGAAGTGGCAACCGGGCTTTTTGGCCATCTGGTGGGGGCGATAGAGGGTGGCTCGGGTTACCGTa                                                      >  2:912981/1‑76 (MQ=255)                    tttttGCCAATGAAGTGGCAACCGGGCTTTTTGGCCATCTGGTGGGGGCGATAGAGGGTGGCTCGGTTTACCGTaa                                                     <  2:1864609/76‑1 (MQ=255)                     ttttGCCAATGAAGTGGCAACCGGGCTTTTTGGCCATCTGGTGGGGGCGATAGAGGGTGGCTCGGTTTACCGCaaa                                                    >  1:1481407/1‑76 (MQ=255)                      tttGCCAATGAAGTGGCAACCGGGCTTTTTGGCCATCTGGTGGGGGCGATAGAGGGTGGCTCGGTTTACCGTAAAt                                                   >  2:160027/1‑76 (MQ=255)                      tttGCCAATGAAGTGGCAACCGGGCTTTTTGGCCATCTGGTGGGGGCGATAGAGGGTGGCTCGGTTTACCGTAAAt                                                   <  2:220198/76‑1 (MQ=255)                            aaTGAAGTGGCAACCGGGCTTTTTGGCCATCTGGTGGGGGCGATAGAGGGTGGCTCGGTTTACCGTAAATCTACCt                                             >  2:1208737/1‑76 (MQ=255)                            aaTGAAGTGGCAACCGGGCTTTTTGGCCATCTGGTGGGGGCGATAGAGGGTGGCTCGGTTTACCGTAAATCTACCt                                             >  2:402835/1‑76 (MQ=255)                              tGAAGTGGCAACCGGGCTTTTTGGCCATCTGGTGGGGGCGATAGAGGGTGGCTCGGTTTACCGTAAATCTACCttt                                           >  2:1467536/1‑76 (MQ=255)                              tGAAGTGGCAACCGGGCTTTTTGGCCATCTGGTGGGGGCGATAGAGGGTGGCTCGGTTTACCGTAAATCTACCttt                                           <  2:1817771/76‑1 (MQ=255)                              tGAAGTGGCAACCGGGCTTTTTGGCCATCTGGTGGGGGCGATAGAGGGTGGCTCGGTTTACCGTAAATCTACCttt                                           <  2:1728181/76‑1 (MQ=255)                               gAAGTGGCAACCGGGCTTTTTGGCCATCTGGTGGGGGCGATAGAGGGTGGCTCGGTTTACCGTAAATCTACCTTTc                                          >  2:590525/1‑76 (MQ=255)                                aaGTGGCGACCGGGCTTTTTGGCCATCTGGTGGGGGCGATAGAGGGTGGCTCGGTTTACCGTAAATCTACCTTTct                                         <  2:802605/76‑1 (MQ=255)                                   tGGCAACCGGGCTTTTTGGCCATCTGGTGGGGGCGATAGAGGGTGGCTCGGTTTACCGTAAATCTACCTTTctgct                                      >  2:1307327/1‑76 (MQ=255)                                   tGGCAACCGGGCTTTTTGGCCATCTGGTGGGGGCGATAGAGGGTGGCTCGGTTTACCGTAAATCTACCTTTctgct                                      <  2:1450680/76‑1 (MQ=255)                                   tGGCAACCGGGCTTTTTGGCCATCTGGTGGGGGCGATAGAGGGTGGCTCGGTTTACCGTAAATCTACCTTTctgct                                      >  2:523347/1‑76 (MQ=255)                                     gcgaCCGGGCTTTTTGGCCATCTGGTGGGGGCGATAGAGGGTGGCTCGGTTTACCGTAAATCTACCTTTCTGCTgg                                    <  1:1891768/73‑1 (MQ=255)                                       aaCCGGGCTTTTTGGCCATCTGGTGGGGGCGATAGAGGGTGGCTCGGTTTACCGTAAATCTACCTTTCTGCTGGan                                  <  1:44326/76‑2 (MQ=255)                                         ccGGGCTTTTTGGCCATCTGGTGGGGGCGATAGAGGGTGGCTCGGTTTACCGTAAATCTACCTTTCTGCTGGATTc                                <  1:1870783/76‑1 (MQ=255)                                           gggCTTTTTGGCCATCTGGTGGGGGCGATAGAGGGTGGCTCGGTTTACCGTAAATCTACCTTTCTGCTGGATTCGc                              >  2:1066721/1‑76 (MQ=255)                                           gggCTTTTTGGCCATCTGGTGGGGGCGATAGAGGGTGGCTCGGTTTACCGTAAATCTACCTTTCTGCTGGATTCGc                              >  2:871717/1‑76 (MQ=255)                                           gggCTTTTTGGCCATCTGGTGGGGGCGATAGAGGGTGGCTCGGTTTACCGTAAATCTACCTTTCTGCTGGATTCGc                              >  2:897096/1‑76 (MQ=255)                                            ggCTTTTTGCCCATCTGGTGGGGGCGATAGAGGGTGGCTCGGTTTACCGTAAATCTACCTTTCTGCTGGATTCGCt                             <  2:971697/76‑1 (MQ=255)                                               tttttGGCCATCTGGTGGGGGCGATAGAGGGTGGCTCGGTTTACCGTAAATCTACCTTTCTGCTGGATTCGCTggg                          >  1:383908/1‑76 (MQ=255)                                                ttttGGCCATCTGGTGGGGGCGATAGAGGGTGGCTCGGTTTACCGTAAATCTACCTTTCTGCTGGATTCGCTGGGt                         <  1:918888/76‑1 (MQ=255)                                                ttttGGCCATCTGGTGGGGGCGATAGAGGGTGGCTCGGTTTACCGTAAATCTACCTTTCTGCTGGATTCGCTGGGt                         >  1:826130/1‑76 (MQ=255)                                                ttttGGCCATCTGGTGGGGGCGATAGAGGGTGGCTCGGTTTACCGTAAATCTACCTTTCTGCTGGATTCGCTGGGt                         <  1:177795/76‑1 (MQ=255)                                                ttttGGCCATCTGGTGGGGGCGATAGAGGGTGGCTCGGTTTACCGTAAATCTACCTTTCTGCTGGATTCGCTGGGt                         <  1:582298/76‑1 (MQ=255)                                                ttttGGCCATCTGGTGGGGGCGATAGAGGGTGGCTCGGTTTACCGTAAATCTACCTTTCTGCTGGATTCGCTGGGt                         <  1:582274/76‑1 (MQ=255)                                                 tttGGCCATCTGGTGGGGGCGATAGAGGGTGGCTCGGTTTACCGTAAATCTACCTTTCTGCTGGATTCGCTGGGTa                        <  2:1103116/76‑1 (MQ=255)                                                 tttGGCCATCTGGTGGGGGCGATAGAGGGTGGCTCGGTTTACCGTAAATCTACCTTTCTGCTGGATTCGCTGGGTa                        >  1:1262739/1‑76 (MQ=255)                                                 tttGGCCATCTGGTGGGGGCGATAGAGGGTGGCTCGGTTTACCGTAAATCTACCTTTCTGCTGGATTCGCTGGGTa                        <  2:1375496/76‑1 (MQ=255)                                                   tGGCCATCTGGTGGGGGCGATAGAGGGTGGCTCGGTTTACCGTAAATCTACCTTTCTGCTGGATTCGCTGGGTaaa                      <  1:1041174/76‑1 (MQ=255)                                                   tGGCCATCTGGTGGGGGCGATAGAGGGTGGCTCGGTTTACCGTAAATCTACCTTTCTGCTGGATTCGCTGGGTaaa                      <  1:1024026/76‑1 (MQ=255)                                                       cATCTGGTGGGGGCGATAGAGGGTGGCTCGGTTTACCGTAAATCTACCTTTCTGCTGGATTCGCTGGGTAAACaaa                  >  1:707865/1‑76 (MQ=255)                                                           tggtggGGGCGATAGAGGGTGGCTCGGTTTACCGTAAATCTACCTTTCTGCTGGATTCGCTGGGTAAACAAATTCt              >  1:689707/1‑76 (MQ=255)                                                             gtggGGGCGATAGAGGGTGGCTCGGTTTACCGTAAATCTACCTTTCTGCTGGATTCGCTGGGTAAACAAATTCTGc            >  1:1839194/1‑76 (MQ=255)                                                              tggGGGCGATAGAGGGTGGCTCGGTTTACCGTAAATCTACCTTTCTGCTGGATTCGCTGGGTAAACAAATTCTGcc           >  2:1190226/1‑76 (MQ=255)                                                               gggggCGATAGAGGGTGGCTCGGTTTACCGTAAATCTACCTTTCTGCTGGATTCGCTGGGTAAACAAATTCTGCCg          >  1:280470/1‑76 (MQ=255)                                                               gggggCGATAGAGGGTGGCTCGGTTTACCGTAAATCTACCTTTCTGCTGGATTCGCTGGGTAAACAAATTCTGCCg          <  1:177751/76‑1 (MQ=255)                                                               gggggCGATAGAGGGTGGCTCGGTTTACCGTAAATCTACCTTTCTGCTGGATTCGCTGGGTAAACAAATTCTGCCg          >  1:1102666/1‑76 (MQ=255)                                                                   gCGATAGAGGGTGGCTCGGTTTACCGTAAATCTACCTTTCTGCTGGATTCGCTGGGTAAACAAATTCTGCCGGACt      <  1:623189/76‑1 (MQ=255)                                                                    cGATAGAGGGTGGCTCGGTTTACCGTAAATCTACCTTTCTGCTGGATTCGCTGGGTAAACAAATTCTGCCGGACTg     <  1:569075/76‑1 (MQ=255)                                                                     gATAGAGGGTGGCTCGGTTTACCGTAAATCTACCTTTCTGCTGGATTCGCTGGGTAAACAAATTCTGCCGGACTgg    >  1:1604586/1‑76 (MQ=255)                                                                      aTAGAGGGTGGCTCGGTTTACCGTAAATCTACCTTTCTGCTGGATTCGCTGGGTAAACAAATTCTGCCGGACTGGc   >  1:48439/1‑76 (MQ=255)                                                                      aTAGAGGGTGGCTCGGTTTACCGTAAATCTACCTTTCTGCTGGATTCGCTGGGTAAACAAATTCTGCCGGACTGGc   >  1:261708/1‑76 (MQ=255)                                                                      aTAGAGGGTGGCTCGGTTTACCGTAAATCTACCTTTCTGCTGGATTCGCTGGGTAAACAAATTCTGCCGGACTGGc   >  1:771200/1‑76 (MQ=255)                                                                      aTAGAGGGTGGCTCGGTTTACCGTAAATCTACCTTTCTGCTGGATTCGCTGGGTAAACAAATTCTGCCGGACTGGc   <  2:1694677/76‑1 (MQ=255)                                                                      aTAGAGGGTGGCTCGGTTTACCGTAAATCTACCTTTCTGCTGGATTCGCTGGGCAAACAAATTCTCCCGGACTGGc   >  1:549694/1‑76 (MQ=255)                                                                       tAGAGGGTGGCTCGGTTTACCGTAAATCTACCTTTCTGCTGGATTCGCTGGGTAAACAAATTCTGCCGGACTGGCt  <  2:1909541/76‑1 (MQ=255)                                                                       tAGAGGGTGGCTCGGTTTACCGTAAATCTACCTTTCTGCTGGATTCGCTGGGTAAACAAATTCTGCCGGACTGGCt  >  1:1021598/1‑76 (MQ=255)                                                                          |                                                                         ACCATGAAAGCGCCAGTCATTTTTGCCAATGAAGTGGCAACCGGGCTTTTTGGCCATCTGGTGGGGGCGATAGCGGGTGGCTCGGTTTACCGTAAATCTACCTTTCTGCTGGATTCGCTGGGTAAACAAATTCTGCCGGACTGGCT  >  REL606/4441930‑4442075

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑