Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	1,305,693	C→A	G183W (GGG→TGG)	cls ←	cardiolipin synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	1,305,693	0	C	A	100.0%	156.6 / NA	40	G183W (GGG→TGG)	cls	cardiolipin synthetase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (19/21);  total (19/21)

AAGGCTTCGACCACTTCAATACCGGCATTACGCATTAGCTCGGGCCACGGGCTGCGGAAAAAAGCCACACTCCCGGCGGAGTCGAGCATCAATCGGCAATGAATGCCGCGTCGCGCAGCGGCCATTAATGATTCAGCCACCTGGTC  >  REL606/1305620‑1305765                                                                          |                                                                         aaGGCTTCGACCACTTCAATACCGGCATTACGCATTAGCTCGGGCCACGGGCTGCGGAAAAAAGCCACACTCCagg                                                                        <  2:830497/76‑1 (MQ=255) aaGGCTTCGACCACTTCAATACCGGCATTACGCATTAGCTCGGGCCACGGGCTGCGGAAAAAAGCCACACTCCagg                                                                        <  2:1661384/76‑1 (MQ=255)  aGGCTTCGACCACTTCAATACCGGCATTACGCATTAGCTCGGGCCACGGGCTGCGGAAAAAAGCCACACTCCaggc                                                                       >  2:1301276/1‑76 (MQ=255)  aGGCTTCGACCACTTCAATACCGGCATTACGCATTAGCTCGGGCCACGGGCTGCGGAAAAAAGCCACACTCCaggc                                                                       >  2:1511536/1‑76 (MQ=255)   ggCTTCGACCACTTCAATACCGCCATTACGCATTAGCTCGGGCCACGGGCTGCGGAAAAAAGCCACACTCCaggcg                                                                      <  2:919321/76‑1 (MQ=255)    gCTTCGACCACTTCAATACCGGCATTACGCATTAGCTCGGGCCACGGGCTGCGGAAAAAAGCCACACTCCaggcgg                                                                     <  2:958488/76‑1 (MQ=255)    gCTTCGACCACTTCAATACCGGCATTACGCATTAGCTCGGGCCACGGGCTGCGGAAAAAAGCCACACGCCaggcgg                                                                     <  2:958477/76‑1 (MQ=255)       tCGACCACTTCAATACCGGCATTACGCATTAGCTCGGGCCACGGGCTGCGGAAAAAAGCCCCACTACAGGCGGGGt                                                                  >  2:331407/1‑76 (MQ=255)         gACCACTTCAATACCGGCATTACGCATTAGCTCGGGCCACGGGCTGCGGAAAAAAGCCACACTCCAGGCGGAGTCg                                                                >  2:656598/1‑76 (MQ=255)         gACCACTTCAATACCGGCATTACGCATTAGCTCGGGCCACGGGCTGCGGAAAAAAGCCACACTCCAGGCGGAGACg                                                                >  1:891118/1‑76 (MQ=255)           ccACTTCAATACCGGCATTACGCATTAGCTCGGGCCACGGGCTGCGGAAAAAAGCCACACTCCAGGCGGAGTCGAg                                                              <  2:1135775/76‑1 (MQ=255)            cACTTCAATACCGGCATTACGCATTAGCTCGGGCCACGGGCTGCGGAAAAAAGCCACACTCCAGGCGGAGTCGAGc                                                             <  1:1035552/76‑1 (MQ=255)            cACTTCAATACCGGCATTACGCATTAGCTCGGGCCACGGGCTGCGGAAAAAAGCCACACTCCAGGCGGAGTCGAGc                                                             >  1:228923/1‑76 (MQ=255)              cTTCAATACCGGCATTACGCATTAGCTCGGGCCACGGGCTGCGGAAAAAAGCCACACTCCAGGCGGAGTCGAGCAt                                                           <  1:1409057/76‑1 (MQ=255)                  aaTACCGGCATTACGCATTAGCTCGGGCCACGGGCTGCGGAAAAAAGCCACACTCCAGGCGGAGTCGAGCATCAAt                                                       <  2:1258467/76‑1 (MQ=255)                  aaTACCGGCATTACGCATTAGCTCGGGCCACGGGCTGCGGAAAAAAGCCACACTCCAGGCGGAGTCGAGCATCAAt                                                       >  1:1821631/1‑76 (MQ=255)                  aaTACCGGCATTACGCATTAGCTCGGGCCACGGGCTGCGGAAAAAAGCCACACTCCAGGCGGAGTCGAGCATCAAt                                                       <  1:397161/76‑1 (MQ=255)                   aTACCGGCATTACGCATTAGCTCGGGCCACGGGCTGCGGAAAAAAGCCACACTCCAGGCGGAGTCGAGCATCAATc                                                      >  2:494609/1‑76 (MQ=255)                   aTACCGGCATTACGCATTAGCTCGGGCCACGGGCTGCGGAAAAAAGCCACACTCCAGGCGGAGTCGAGCATCAATc                                                      <  1:910388/76‑1 (MQ=255)                    tACCGGCATTACGCATTAGCTCGGGCCACGGGCTGCGGAAAAAAGCCACACTCCAGGCGGAGTCGAGCATCAATCg                                                     <  1:33693/76‑1 (MQ=255)                    tACCGGCATTACGCATTAGCTCGGGCCACGGGCTGCGGAAAAAAGCCACACTCCAGGCGGAGTCGAGCATCAATCg                                                     <  1:1272194/76‑1 (MQ=255)                       cGGCATTACGCATTAGCTCGGGCCACGGGCTGCGGAAAAAAGCCACACTCCAGGCGGAGTCGAGCATCAATCGGCa                                                  >  1:1788449/1‑76 (MQ=255)                           aTTACGCATTAGCTCGGGCCACGGGCTGCGGAAAAAAGCCACACTCCAGGCGGAGTCGAGCATCAATCGGCaatga                                              <  2:127854/76‑1 (MQ=255)                             tACGCATTAGCTCGGGCCACGGGCTGCGGAAAAAAGCCACACTCCAGGCGGAGTCGAGCATCAATCGGCaatgaat                                            <  1:1527944/76‑1 (MQ=255)                              aCGCATTAGCTCGGGCCACGGGCTGCGGAAAAAAGCCACACTCCAGGCGGAGTCGAGCATCAATCGGCaatgaatg                                           >  2:1405160/1‑76 (MQ=255)                               cGCATTAGCTCGGGCCACGGGCTGCGGAAAAAAGCCACACTCCAGGCGGAGTCGAGCATCAATCGGCAATGAATGc                                          <  1:481863/76‑1 (MQ=255)                                 cATTAGCTCGGGCCACGGGCTGCGGAAAAAAGCCACACTCCAGGCGGAGTCGAGCATCAATCGGCAATGAATGCcg                                        >  1:508456/1‑76 (MQ=255)                                  aTTAGCTCGGGCCACGGGCTGCGGAAAAAAGCCACACTCCAGGCGGAGTCGAGCATCAATCGGCAATGAATGCcgn                                       <  1:1719402/76‑2 (MQ=255)                                       cgcGGGCCAGGGGCTGCGGAAAAAAGCCACACTCCAGGCGGAGTCGAGCATCAATCGGCAATGAATGCCGCGTcgc                                  <  1:847041/74‑1 (MQ=255)                                          gggCCACGGGCTGCGGAAAAAAGCCACACTCCAGGCGGAGTCGAGCATCAATCGGCAATGAATGCCGCGTCGCGCa                               <  2:1746352/76‑1 (MQ=255)                                              cACGGGCTGCGGAAAAAAGCCACACTCCAGGCGGAGTCGAGCATCAATCGGCAATGAATGCCGCGTCGCGCAGCgg                           >  2:1914267/1‑76 (MQ=255)                                               aCGGGCTGCGGAAAAAAGCCACACTCCAGGCGGAGTCGAGCATCAATCGGCAATGAATGCCGCGTCGCGCAGCGGc                          >  1:1938225/1‑76 (MQ=255)                                                cGGGCTGCGGAAAAAAGCCACACTCCAGGCGGAGTCGAGCATCAATCGGCAATGAATGCCGCGTCGCGCAGCGGcc                         <  1:1432825/76‑1 (MQ=255)                                                   gCTGCGGAAAAAAGCCACACTCCAGGCGGAGTCGAGCATCAATCGGCAATGAATGCCGCGTCGCGCAGCGGCCAtt                      <  1:1102336/76‑1 (MQ=255)                                                   gCTGCGGAAAAAAGCCACACTCCAGGCGGAGTCGAGCATCAATCGGCAATGAATGCCGCGTCGCGCAGCGGCCAtt                      >  2:106713/1‑76 (MQ=255)                                                     tGCGGAAAAAAGCCACACTCCAGGCGGAGTCGAGCATCAATCGGCAATGAATGCCGCGTCGCGCAGCGGCCATTaa                    <  2:1434578/76‑1 (MQ=255)                                                     tGCGGAAAAAAGCCACACTCCAGGCGGAGTCGAGCATCAATCGGCAATGAATGCCGCGTCGCGCAGCGGCCATTaa                    <  2:503754/76‑1 (MQ=255)                                                      gCGGAAAAAAGCCACACTCCAGGCGGAGTCGAGCATCAATCGGCAATGAATGCCGCGTCGCGCAGCGGCCATTAAt                   >  1:176307/1‑76 (MQ=255)                                                      gCGGAAAAAAGCCACACTCCAGGCGGAGTCGAGCATCAATCGGCAATGAATGCCGCGTCGCGCAGCGGCCATTAAt                   >  2:335351/1‑76 (MQ=255)                                                             aaaGCCACACTCCAGGCGGAGTCGAGCATCAATCGGCAATGAATGCCGCGTCGCGCAGCGGCCATTAATGATTCAg            <  1:603710/76‑1 (MQ=255)                                                              aaGCCACACTCCAGGCGGAGTCGAGCATCAATCGGCAATGAATGCCGCGTCGCGCAGCGGCCATTAATGATTCAGc           >  2:656810/1‑76 (MQ=255)                                                               acccACCCTCCAGGCGGAGTCGAGCATCAATCGGCAATGAATGCCGCGTCGCGCAGCGGCCATTAATGATTCAGcc          <  1:1731384/74‑1 (MQ=255)                                                               aGCCACACTCCAGGCGGAGTCGAGCATCAATCGGCAATGAATGCCGCGTCGCGCAGCGGCCATTAATGATTCAGcc          <  1:1731388/76‑1 (MQ=255)                                                                 ccACACTCCAGGCGGAGTCGAGCATCAATCGGCAATGAATGCCGCGTCGCGCAGCGGCCATTAATGATTCAGCCAc        >  1:1296882/1‑76 (MQ=255)                                                                  cacaCTCCAGGCGGAGTCGAGCATCAATCGGCAATGAATGCCGCGTCGCGCAGCGGCCATTAATGATTCAGCCAcc       >  2:276017/1‑76 (MQ=255)                                                                  cacaCTCCAGGCGGAGTCGAGCATCAATCGGCAATGAATGCCGCGTCGCGCAGCGGCCATTAATGATTCAGCCAcc       >  1:242616/1‑76 (MQ=255)                                                                   acacTCCAGGCGGAGTCGAGCATCAATCGGCAATGAATGCCGCGTCGCGCAGCGGCCATTAATGATTCAGCCACCt      >  2:1128031/1‑76 (MQ=255)                                                                    cgcTCCAGGGGGAGTCGAGCATCAATCGGCAATGAATGCCGCGTCGCGCAGCGGCCATTAATGATTCAGCCACCTg     <  1:1094993/74‑1 (MQ=255)                                                                      cTCCAGGCGGAGTCGAGCATCAATCGGCAATGAATGCCGCGTCGCGCAGCGGCCATTAATGATTCAGCCACCTGGt   >  2:1415648/1‑76 (MQ=255)                                                                       tCCAGGCGGAGTCGAGCATCAATCGGCAATGAATGCCGCGTCGCGCAGCGGCCATTAATGATTCAGCCACCTGGTc  <  1:1511869/76‑1 (MQ=255)                                                                       tCCAGGCGGAGTCGAGCATCAATCGGCAATGAATGCCGCGTCGCGCAGCGGCCATTAATGATTCAGCCACCTGGTc  <  2:208811/76‑1 (MQ=255)                                                                          |                                                                         AAGGCTTCGACCACTTCAATACCGGCATTACGCATTAGCTCGGGCCACGGGCTGCGGAAAAAAGCCACACTCCCGGCGGAGTCGAGCATCAATCGGCAATGAATGCCGCGTCGCGCAGCGGCCATTAATGATTCAGCCACCTGGTC  >  REL606/1305620‑1305765

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑