Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	2,193,779	G→T	L143I (CTT→ATT)	yeiB ←	conserved inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	2,193,779	0	G	T	100.0%	218.0 / NA	63	L143I (CTT→ATT)	yeiB	conserved inner membrane protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (34/29);  total (34/29)

GCCGATGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAAGCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAGCCGCCA  >  REL606/2193706‑2193851                                                                          |                                                                         gCCGATGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATcc                                                                        >  2:611795/1‑76 (MQ=255)  ccGATGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATccc                                                                       >  2:812923/1‑76 (MQ=255)   cGATGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATcccc                                                                      >  2:779611/1‑76 (MQ=255)   cGATGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATcccc                                                                      >  1:1775265/1‑76 (MQ=255)   cGATGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATcccc                                                                      >  1:297311/1‑76 (MQ=255)    gATGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCcca                                                                     >  2:1745648/1‑76 (MQ=255)    gATGCATCCGGCGTCCAGGCGCGGCTGGGCTGGCTGGCGGAAATCAACCCCAAAAAGCAAAAAACGCCAAGccacc                                                                     >  2:104869/1‑73 (MQ=255)    gATGCACCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCcca                                                                     <  1:1307626/76‑1 (MQ=255)      tGCGTCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCccacc                                                                   <  2:285747/76‑1 (MQ=255)       gCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCccacca                                                                  <  1:1395580/76‑1 (MQ=255)        cagccGGCGGCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAg                                                                 <  2:1836831/73‑1 (MQ=255)            cGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATaa                                                             <  1:204278/76‑1 (MQ=255)              gCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAg                                                           <  2:408444/76‑1 (MQ=255)               cGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGc                                                          <  1:1169750/76‑1 (MQ=255)                   cAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGa                                                      <  1:764072/76‑1 (MQ=255)                    aGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGAc                                                     <  1:1042361/76‑1 (MQ=255)                       cgcgGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGcc                                                  <  2:841417/76‑1 (MQ=255)                        gcgGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCt                                                 >  2:1165703/1‑76 (MQ=255)                          ggCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGt                                               <  2:270089/76‑1 (MQ=255)                             tGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTAtt                                            >  2:311860/1‑76 (MQ=255)                             tGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTAtt                                            >  2:1468481/1‑76 (MQ=255)                               gTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTaa                                          <  1:439510/76‑1 (MQ=255)                                tCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTaaa                                         >  1:254693/1‑76 (MQ=255)                                 cTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAAc                                        >  1:111698/1‑76 (MQ=255)                                 cTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAAc                                        >  1:329927/1‑76 (MQ=255)                                   ggCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAg                                      <  1:779611/76‑1 (MQ=255)                                     cTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGc                                    <  2:1461137/76‑1 (MQ=255)                                       gTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCtt                                  >  2:228614/1‑76 (MQ=255)                                        tCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCttt                                 <  2:311211/76‑1 (MQ=255)                                        tCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCttt                                 <  2:1291120/76‑1 (MQ=255)                                        tCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCttt                                 <  2:1467584/76‑1 (MQ=255)                                         cGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCtttt                                >  1:1576898/1‑76 (MQ=255)                                         cGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCtttt                                <  2:808925/76‑1 (MQ=255)                                          ggAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCttttt                               <  2:550929/76‑1 (MQ=255)                                          ggAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCttttt                               <  2:29403/76‑1 (MQ=255)                                          ggAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCttttt                               <  2:1252812/76‑1 (MQ=255)                                           gAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTa                              <  2:126675/76‑1 (MQ=255)                                            aaaTCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTAc                             >  1:1267738/1‑76 (MQ=255)                                            aaaTCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTAc                             >  2:1926261/1‑76 (MQ=255)                                            aaaTCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTAc                             >  2:275875/1‑76 (MQ=255)                                            aaaTCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTAc                             >  2:1782198/1‑76 (MQ=255)                                                cAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGAt                         >  2:1218376/1‑76 (MQ=255)                                                      cAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCa                   >  1:846469/1‑76 (MQ=255)                                                      cAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCa                   >  1:1468937/1‑76 (MQ=255)                                                      cAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCa                   <  2:1502641/76‑1 (MQ=255)                                                       aaTAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCTTGGATTATACAGGCTTTTTACCTATGtcgtgt                  >  2:1799590/1‑73 (MQ=255)                                                       aaTAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCAt                  >  1:821373/1‑76 (MQ=255)                                                        aTAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATc                 >  2:658748/1‑76 (MQ=255)                                                        aTAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATc                 >  2:807129/1‑76 (MQ=255)                                                        aTAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATc                 >  1:1177899/1‑76 (MQ=255)                                                        aTAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATc                 >  2:262644/1‑76 (MQ=255)                                                        aTAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATc                 >  2:1716417/1‑76 (MQ=255)                                                         tAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATcg                >  2:1615456/1‑76 (MQ=255)                                                           aCAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATcgcg              <  2:1043452/76‑1 (MQ=255)                                                                aaaaCGCCAATCCCCACCAGATAGAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCa         <  2:409083/76‑1 (MQ=255)                                                                aaaaCGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCTATGGCGCATCGTGCTccg         >  2:1770393/1‑75 (MQ=255)                                                                aaaaCGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCa         >  2:183523/1‑76 (MQ=255)                                                                 aaaCGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAg        >  1:403630/1‑76 (MQ=255)                                                                  aaCGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAgc       >  1:586172/1‑76 (MQ=255)                                                                    cGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAgccg     <  2:1613864/76‑1 (MQ=255)                                                                      ccAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAgccgcc   <  1:262644/76‑1 (MQ=255)                                                                      ccAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAgccgcc   <  1:239350/76‑1 (MQ=255)                                                                      ccAACCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAgccgcc   <  1:1716417/76‑1 (MQ=255)                                                                      ccAACCCCCACCAAAAAAAGCATGCCGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAgccgcc   <  1:658748/76‑1 (MQ=255)                                                                       cAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAGCCGCca  >  2:433449/1‑76 (MQ=255)                                                                       cAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAGCCGCca  <  2:711842/76‑1 (MQ=255)                                                                          |                                                                         GCCGATGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAAGCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAGCCGCCA  >  REL606/2193706‑2193851

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑