| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 2,762,214 | A→G | Y67H (TAT→CAT) | rpoS ← | RNA polymerase sigma factor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 2,762,214 | 0 | A | G | 97.7% | 177.8 / ‑3.9 | 44 | Y67H (TAT→CAT) | rpoS | RNA polymerase sigma factor |
| Reads supporting (aligned to +/- strand): ref base A (0/1); new base G (28/15); total (28/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.64e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.83e-01 | |||||||||||
CGAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCTCCCTGCGATAACAGTTC > REL606/2762141‑2762283 | cgAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATGAc < 2:1427015/76‑1 (MQ=255)cgAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATGAc > 2:1646362/1‑76 (MQ=255) agGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATGACCAAt > 2:260221/1‑76 (MQ=255) cGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATGACCAAtctc > 1:1524177/1‑76 (MQ=255) cGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATGACCAAtctc < 2:143799/76‑1 (MQ=255) gACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATAACCAATCTCa < 1:1489620/76‑1 (MQ=255) aCATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATGACCAATCTCAc < 2:172153/76‑1 (MQ=255) cATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATGACCAATCTCAcc > 2:1779658/1‑76 (MQ=255) aTCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATGACCAATCTCACCa > 1:253489/1‑76 (MQ=255) ctcCACGCGGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATGACCAATCTCACCAAg < 1:1739816/76‑1 (MQ=255) ctcCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATGACCAATCTCACCAAg < 1:739045/76‑1 (MQ=255) ccACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATGACCAATCTCACCAAGGt < 1:297975/76‑1 (MQ=255) ccACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATGACCAATCTCACCAAGGt < 1:740600/76‑1 (MQ=255) ccACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATGACCAATCTCACCAAGGt < 2:307149/76‑1 (MQ=255) ccACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATGACCAATCTCACCAAGGt > 2:1082051/1‑76 (MQ=255) cACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATGACCAATCTCACCAAGGTa > 1:775082/1‑76 (MQ=255) cACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATGACCAATCTCACCAAGGTa > 2:359226/1‑76 (MQ=255) cgcgACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATGACCAATCTCACCAAGGTAAAGCTGAGt > 2:1613228/1‑76 (MQ=255) gcgACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATGACCAATCTCACCAAGGTAAAGCTGAGTc < 1:482692/76‑1 (MQ=255) aaaaTAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATGACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAac > 2:1897849/1‑76 (MQ=255) aaaaTAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATGACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAac > 1:1949157/1‑76 (MQ=255) aaaaTAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATGACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAac > 1:1192664/1‑76 (MQ=255) aaTAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATGACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAacac > 1:1193227/1‑76 (MQ=255) aTAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATGACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAacaca > 2:538427/1‑76 (MQ=255) aaaCTTCTTCTTCGGCCGTTAACAGTGGTGAATGACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACg > 2:608564/1‑76 (MQ=255) aaaCTTCTTCTTCGGCCGTTAACAGTGGTGAATGACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACg > 1:1879489/1‑76 (MQ=255) aaCTTCTTCTTCGGCCGTTAACAGTGGTGAATGACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGc < 1:700914/76‑1 (MQ=255) cttcttcttCGGCCGTTAACAGTGGTGAATGACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCtg < 2:1481186/76‑1 (MQ=255) tcttcttcGGCCGTTAACAGTGGTGAATGACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCtgtg > 1:548772/1‑76 (MQ=255) tcttcttcGGCCGTTAACAGTGGTGAATGACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCtgtg > 1:504043/1‑76 (MQ=255) ttcttcGGCCGTTAACAGTGGTGAATGACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCtgtgtg < 2:1076524/76‑1 (MQ=255) ttcttcGGCCGTTAACAGTGGTGAATGACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCtgtgtg < 1:520363/76‑1 (MQ=255) ttcGGCCGTTAACAGTGGTGAATGACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCt < 2:1388414/76‑1 (MQ=255) tcGGCCGTTAACAGTGGTGAATGACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGCGTGGCTc > 2:652942/1‑76 (MQ=255) gCCGTTAACAGTGGTGAATGACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCTCCCt < 2:462634/76‑1 (MQ=255) cGTTAACAGTGGTGAATGACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCTCCCTGc > 2:257258/1‑76 (MQ=255) ttAACAGTGGTGAATGACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCTCCCTGCGa > 1:413118/1‑76 (MQ=255) ttAACAGTGGTGAATGACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCTCCCTGCGa > 2:1270526/1‑76 (MQ=255) aaCAGTGGTGAATGACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCTCCCTGCGATa > 2:1842661/1‑76 (MQ=255) aaCAGTGGTGAATGACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCTCCCTGCGATa > 1:681399/1‑76 (MQ=255) aGTGGTGAATGACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCTCCCTGCGATAACa > 1:1198893/1‑76 (MQ=255) gtggtgAATGACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCTCCCTGCGATAACAg > 1:15414/1‑76 (MQ=255) tggtgAATGACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCTCCCTGCGATAACAGt > 2:1952920/1‑76 (MQ=255) gtgAATGACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCTCCCTGCGATAACAGTTc > 2:490740/1‑76 (MQ=255) | CGAGAGGCGACATCTCCACGCAGTGCGCGACGCGCAAAATAAACTTCTTCTTCGGCCGTTAACAGTGGTGAATAACCAATCTCACCAAGGTAAAGCTGAGTCGCGTCCAACACACGCTGTGTGGCTCCCTGCGATAACAGTTC > REL606/2762141‑2762283 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |