Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	REL606	3,015,771	IS150 (–) +3 bp	coding (1192‑1194/1677 nt)	ECB_02816 →	KpsD protein

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	REL606	= 2775877	NA (NA)	49 (0.860)	41/146	0.3	95.9%	noncoding (1/1443 nt)	IS150	repeat region
	?	REL606	3015771 =	2 (0.040)					coding (1192/1677 nt)	ECB_02816	KpsD protein
*	?	REL606	= 3015773	2 (0.040)	82 (1.420)	51/148	0.1	97.5%	coding (1194/1677 nt)	ECB_02816	KpsD protein
	?	REL606	= 3894996	NA (NA)					noncoding (1443/1443 nt)	IS150	repeat region

TATCGCCCATCAGTGGCTCAGCGTCAGAAAGAGATGCTGAATCTCTCGCTGCAAAAGCTGGAGGAAGCCTCACTTt‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  REL606/3015699‑3015773 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑TGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATATTCAATTGGAGTCAGA  <  REL606/3894996‑3894922                                                                                                                                                        TATCGCCCATCAGTGGCTCAGCGTCAGAAAGAGATGCTGAATCTCTCGCTGCAAAAGCTGGAGGAAGCCTCACTTT                                                                            >  2:1146349/1‑76  ATCGCCCATCAGTGGCTCAGCGTCAGAAAGAGATGCTGAATCTCTCGCTGCAAAAGCTGGAGGAAGCCTCACTTTG                                                                           >  1:1129547/1‑76  ATCGCCCATCAGTGGCTCAGCGTCAGAAAGAGATGCTGAATCTCTCGCTGCAAAAGCTGGAGGAAGCCTCACTTTG                                                                           >  2:471870/1‑76  ATCGCCCATCAGTGGCTCAGCGTCAGAAAGAGATGCTGAATCTCTCGCTGCAAAAGCTGGAGGAAGCCTCACTTTG                                                                           >  2:471863/1‑76   TCGCCCATCAGTGGCTCAGCGTCAGAAAGAGATGCTGAATCTCTCGCTGCAAAAGCTGGAGGAAGCCTCACTTTGT                                                                          <  1:417292/76‑1   TCGCCCATCAGTGGCTCAGCGTCAGAAAGAGATGCTGAATCTCTCGCTGCAAAAGCTGGAGGAAGCCTCACTTTGT                                                                          <  2:1863167/76‑1   TCGCCCATCAGTGGCTCAGCGTCAGAAAGAGATGCTGAATCTCTCGCTGCAAAAGCTGGAGGAAGCCTCACTTTGT                                                                          <  1:1510378/76‑1    CGCCCATCAGTGGCTCAGCGTCAGAAAGAGATGCTGAATCTCTCGCTGCAAAAGCTGGAGGAAGCCTCACTTTGTA                                                                         >  2:993770/1‑76     GCCCATCAGTGGCTCAGCGTCAGAAAGAGATGCTGAATCTCTCGCTGCAAAAGCTGGAGGAAGCCTCACTTTGTAC                                                                        <  2:182761/76‑1      CCCATCAGTGGCTCAGCGTCAGAAAGAGATGCTGAATCTCTCGCTGCAAAAGCTGGAGGAAGCCTCACTTTGTACT                                                                       <  1:1158360/76‑1      CCCATCAGTGGCTCAGCGTCAGAAAGAGATGCTGAATCTCTCGCTGCAAAAGCTGGAGGAAGCCTCACTTTGTACT                                                                       >  2:1112467/1‑76               GGCTCAGCGTCAGAAAGAGATGCTGAATCTCTCGCTGCAAAAGCTGGAGGAAGCCTCACTTTGTACTGACCCCAAA                                                              <  1:134033/76‑1                 CTCAGCGTCAGAAAGAGATGCTGAATCTCTCGCTGCAAAAGCTGGAGGAAGCCTCACTTTGTACTGACCCCAAAAA                                                            <  2:461229/76‑1                 CTCAGCGTCAGAAAGAGATGCTGAATCTCTCGCTGCAAAAGCTGGAGGAAGCCTCACTTTGTACTGACCCCAAAAA                                                            >  1:289591/1‑76                 CTCAGCGTCAGAAAGAGATGCTGAATCTCTCGCTGCAAAAGCTGGAGGAAGCCTCACTTTGTACTGACCCCAAAAA                                                            >  1:1669469/1‑76                 CTCAGCGTCAGAAAGAGATGCTGAATCTCTCGCTGCAAAAGCTGGAGGAAGCCTCACTTTGTACTGACCCCAAAAA                                                            >  2:89717/1‑76                  TCAGCGTCAGAAAGAGATGCTGAATCTCTCGCTGCAAAAGCTGGAGGAAGCCTCACTTTGTACTGACCCCAAAAAG                                                           >  2:164070/1‑76                          AGAAAGAGATGCTGAATCTCTCGCTGCAAAAGCTGGAGGAAGCCTCACTTTGTACTGACCCCAAAAAGTTGGACAG                                                   >  2:806187/1‑76                          AGAAAGAGATGCTGAATCTCTCGCTGCAAAAGCTGGAGGAAGCCTCACTTTGTACTGACCCCAAAAAGTTGGACAG                                                   >  2:933355/1‑76                            AAAGAGATGCTGAATCTCTCGCTGCAAAAGCTGGAGGAAGCCTCACTTTGTACTGACCCCAACAAGTTGGACAGTT                                                 >  2:285667/1‑76                            AAAGAGATGCTGAATCTCTCGCTGCAAAAGCTGGAGGAAGCCTCACTTTGTACTGACCCCAAAAAGTTGGACAGTT                                                 >  2:515567/1‑76                            AAAGAGATGCTGAATCTCTCGCTGCAAAAGCTGGAGGAAGCCTCACTTTGTACTGACCCCAAAAAGTTGGACAGTT                                                 >  2:296981/1‑76                            AAAGAGATGCTGAATCTCTCGCTGCAAAAGCTGGAGGAAGCCTCACTTTGTACTGACCCCAAAAAGTTGGACAGTT                                                 >  2:1407563/1‑76                            AAAGAGATGCTGAATCTCTCGCTGCAAAAGCTGGAGGAAGCCTCACTTTGTACTGACCCCAAAAAGTTGGACAGTT                                                 >  2:296963/1‑76                             AAGAGATGCTGAATCTCTCGCTGCAAAAGCTGGAGGAAGCCTCACTTTGTACTGACCCCAAAAAGTTGGACAGTTA                                                <  2:585624/76‑1                             AAGAGATGCTGAATCTCTCGCTGCAAAAGCTGGAGGAAGCCTCACTTTGTACTGACCCCAAAAAGTTGGACAGTTA                                                >  2:1445785/1‑76                              AGAGATGCTGAATCTCTCGCTGCAAAAGCTGGAGGAAGCCTCACTTTGTACTGACCCCAAAAAGTTGGACAGTTAA                                               <  1:883091/76‑1                                AGATGCTGAATCTCTCGCTGCAAAAGCTGGAGGAAGCCTCACTTTGTACTGACCCCAAAAAGTTGGACAGTTAAAC                                             >  2:642508/1‑76                                AGATGCTGAATCTCTCGCTGCAAAAGCTGGAGGAAGCCTCACTTTGTACTGACCCCAAAAAGTTGGACAGTTAAAC                                             >  2:1564815/1‑76                                 GATGCTGAATCTCTCGCTGCAAAAGCTGGAGGAAGCCTCACTTTGTACTGACCCCAAAAAGTTGGACAGTTAAACA                                            <  2:1015125/76‑1                                 GATGCTGAATCTCTCGCTGCAAAAGCTGGAGGAAGCCTCACTTTGTACTGACCCCAAAAAGTTGGACAGTTAAACA                                            <  1:1819710/76‑1                                  ATGCTGAATCTCTCGCTGCAAAAGCTGGAGGAAGCCTCACTTTGTACTGACCCCAAAAAGTTGGACAGTTAAACAC                                           <  1:1769622/76‑1                                   TGCTGAATCTCTCGCTGCAAAAGCTGGAGGAAGCCTCACTTTGTACTGACCCCAAAAAGTTGGACAGTTAAACACG                                          <  2:158736/76‑1                                   TGCTGAATCTCTCGCTGCAAAAGCTGGAGGAAGCCTCACTTTGTACTGACCCCAAAAAGTTGGACAGTTAAACACG                                          <  1:1839498/76‑1                                   TGCTGAATCTCTCGCTGCAAAAGCTGGAGGAAGCCTCACTTTGTACTGACCCCAAAAAGTTGGACAGTTAAACACG                                          >  1:919145/1‑76                                        AATCTCTCGCTGCAAAAGCTGGAGGAAGCCTCACTTTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCA                                     >  2:300641/1‑76                                        AATCTCTCGCTGCAAAAGCTGGAGGAAGCCTCACTTTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCA                                     >  2:1024727/1‑76                                        AATCTCTCGCTGCAAAAGCTGGAGGAAGCCTCACTTTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCA                                     >  1:1135417/1‑76                                        AATCTCTCGCTGCAAAAGCTGGAGGAAGCCTCACTTTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCA                                     >  1:1724837/1‑76                                        AATCTCTCGCTGCAAAAGCTGGAGGAAGCCTCACTTTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCA                                     <  1:280565/76‑1                                         ATCTCTCGCTGCAAAAGCTGGAGGAAGCCTCACTTTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCAT                                    >  1:1748256/1‑76                                            TCTCGCTGCAAAAGCTGGAGGAAGCCTCACTTTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATA                                 <  1:527309/76‑1                                             CTCGCTGCAAAAGCTGGAGGAAGCCTCACTTTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAG                                >  1:1423205/1‑76                                             CTCGCTGCAAAAGCTGGAGGAAGCCTCACTTTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAG                                <  2:523107/76‑1                                             CTCGCTGCAAAAGCTGGAGGAAGCCTCACTTTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAG                                <  1:341159/76‑1                                             CTCGCTGCAAAAGCTGGAGGAAGCCTCACTTTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAG                                <  2:1574363/76‑1                                              TCGCTGCAAAAGCTGGAGGAAGCCTCACTTTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGG                               <  1:1600804/76‑1                                                GCTGCAAAAGCTGGAGGAAGCCTCACTTTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTC                             <  1:993770/76‑1                                                GCTGCAAAAGCTGGAGGAAGCCTCACTTTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTC                             >  1:1557572/1‑76                                                GCTGCAAAAGCTGGAGGAAGCCTCACTTTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTC                             <  2:166680/76‑1                                                 CTGCAAAAGCTGGAGGAAGCCTCACTTTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCT                            >  1:292260/1‑76                                                  TGCAAAAGCTGGAGGAAGCCTCTCTTTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTG                           <  2:374961/76‑1                                                  TGCAAAAGCTGGAGGAAGCCTCACTTTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTG                           >  1:999219/1‑76                                                   GCAAAAGCTGGAGGAAGCCTCACTTTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGA                          >  2:166594/1‑76                                                   GCAAAAGCTGGAGGAAGCCTCACTTTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGA                          <  2:1051271/76‑1                                                     AAAAGCTGGAGGAAGCCTCACTTTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATT                        >  1:771711/1‑76                                                     AAAAGCTGGAGGAAGCCTCACTTTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATT                        <  2:971845/76‑1                                                       AAGCTGGAGGAAGCCTCACTTTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCC                      <  2:1236954/76‑1                                                         GCTGGAGGAAGCCTCACTTTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGA                    <  2:73570/76‑1                                                          CTGGAGGAAGCCTCACTTTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGAT                   <  1:1468036/76‑1                                                           TGGAGGAAGCCTCACTTTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATA                  >  2:284415/1‑76                                                            GGAGGAAGCCTCACTTTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATAT                 >  1:120169/1‑76                                                            GGAGGAAGCCTCACTTTGTACTGACCCCAAAAAGTTGGACAGTTAAACACCAGGCATATAGGTCTGATTCCGATAT                 >  2:1614657/1‑76                                                             GAGGAAGCCTCACTTTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATATT                <  1:970340/76‑1                                                              AGGAAGCCTCACTTTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATATTC               >  1:953092/1‑76                                                                GAAGCCTCACTTTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATATTCAA             >  2:1515385/1‑76                                                                  AGCCTCACTTTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATATTCAATT           >  2:1462774/1‑76                                                                   GCCTCACTTTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCCGATTCCGATATTCAATTG          >  1:1367273/1‑76                                                                    CCTCACTTTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATATTCAATTGG         <  2:1037023/76‑1                                                                    CCTCACTTTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATATTCAATTGG         <  2:700035/76‑1                                                                       CACTTTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATATTCAATTGGAGT      >  2:1740295/1‑76                                                                       CACTTTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATATTCAATTGGAGT      >  2:501440/1‑76                                                                       CACTTTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATATTCAATTGGAGT      >  1:546313/1‑76                                                                        ACTTTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATATTCAATTGGAGTC     <  2:1378170/76‑1                                                                        ACTTTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATATTCAATTGGAGTC     <  1:881480/76‑1                                                                        ACTTTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATATTCAATTGGAGTC     <  1:1423914/76‑1                                                                        ACTTTGTACTGACCCCAAAAAGTTGGACAGCTAAACACGAGGCATATAGGTCTGATTCCGATATTCAATTGGAGTC     >  1:1146170/1‑76                                                                         CTTTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATATTCAATTGGAGTCA    <  2:1354755/76‑1                                                                          TTTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATATTCAATTGGAGTCAG   <  1:1476510/76‑1                                                                          TTTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATATTCAATTGGAGTCAG   <  1:1771119/76‑1                                                                          TTTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATATTCAATTGGAGTCAG   >  1:56188/1‑76                                                                           TTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATATTCAATTGGAGTCAGA  <  2:602727/76‑1                                                                           TTGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATATTCAATTGGAGTCAGA  <  1:353818/76‑1                                                                                                                                                        TATCGCCCATCAGTGGCTCAGCGTCAGAAAGAGATGCTGAATCTCTCGCTGCAAAAGCTGGAGGAAGCCTCACTTt‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  REL606/3015699‑3015773 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑TGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATATTCAATTGGAGTCAGA  <  REL606/3894996‑3894922

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.