| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 473,924 | A→C | V461G (GTC→GGC) | ybaL ← | predicted transporter with NAD(P)‑binding Rossmann‑fold domain |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 473,924 | 0 | A | C | 96.7% | 224.4 / ‑1.2 | 60 | V461G (GTC→GGC) | ybaL | predicted transporter with NAD(P)‑binding Rossmann‑fold domain |
| Reads supporting (aligned to +/- strand): ref base A (1/1); new base C (26/32); total (27/33) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.31e-01 | |||||||||||
GCACATTCCAGATGCGCCAGTTG‑CATAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGACCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGTCTCAATCACCACCAGCGGAATATCAGAGGCGA > REL606/473861‑473996 | gCACATTCCAGATGCGCCAGTTG‑CATAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGCCCCCGCGCtctc < 2:81371/76‑1 (MQ=255) cacaTTCCAGATGCGCCAGTTG‑CATAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGCCCCCTCGCTCTCg > 1:400967/1‑76 (MQ=255) acaTTCCAGATGCGCCAGTTG‑CATAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGc < 2:990588/76‑1 (MQ=255) acaTTCCAGATGCGCCAGTTG‑CATAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGc < 2:812877/76‑1 (MQ=255) acaTTCCAGATGCGCCAGTTG‑CATAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGc > 1:348389/1‑76 (MQ=255) acaTTCCAGATGCGCCAGTTG‑CATAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGc < 2:1129595/76‑1 (MQ=255) acaGTCCAGATGCGCCAGTTG‑CATAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGc < 2:557000/76‑1 (MQ=255) ccatCCAGGAGCCCCAGTTGCCATAATTTCTCCGTCCCCCGCATCCCCCAATCCGGCGCGCCCCCCGCGCTCTCGc < 2:288951/73‑1 (MQ=255) caTTCCAGATGCGCCAGTTG‑CATAATTTCTTCGTGCGCCGCATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCa < 2:743352/76‑1 (MQ=255) aTTCCAGATGCGCCAGTTG‑CATAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGcgg > 1:1335543/1‑74 (MQ=255) tCCAGATGCGCCAGTTG‑CATAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCt < 2:517057/76‑1 (MQ=255) tCCAGATGCGCCAGTTG‑CATAATTACTTCGTTCGCCGCATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCt < 2:378425/76‑1 (MQ=255) ccAGATGCGCCAGTTG‑CATAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCtc > 1:1249266/1‑76 (MQ=255) ccAGATGCGCCAGTTG‑CATAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGACCCCGCGCTCTCGCAGCtc < 2:811792/76‑1 (MQ=255) aGATGCGCCAGTTG‑CATAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCtcat < 1:1338504/76‑1 (MQ=255) aGATGCGCCAGTTG‑CATAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCtcat < 1:383839/76‑1 (MQ=255) aGATGCGCCAGTTG‑CATAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCtcat > 1:1054773/1‑76 (MQ=255) gATGCGCCAGTTG‑CATAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCtcatc < 2:1502424/76‑1 (MQ=255) tGCGCCAGTTG‑CATAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCaa > 1:643845/1‑76 (MQ=255) gcgcCAGTTG‑CATAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAac < 1:956408/76‑1 (MQ=255) gcgcCAGTTG‑CATAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAac < 2:491642/76‑1 (MQ=255) gcgcCAGTTG‑CATAATGTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAac < 2:1631278/76‑1 (MQ=255) cgcCAGTTG‑CATAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAaca < 1:1558795/76‑1 (MQ=255) aGTTG‑CATAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACggg < 1:121996/76‑1 (MQ=255) ttG‑CAAAATTCCTGCGTTCGCCGCACTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGtt < 2:124842/76‑1 (MQ=255) tG‑CATAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTc < 2:1170213/76‑1 (MQ=255) tG‑CATAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTc > 1:66214/1‑76 (MQ=255) tG‑CATAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGAGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTc > 2:54236/1‑76 (MQ=255) cATAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGt > 2:1204095/1‑76 (MQ=255) cATAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGt > 2:794124/1‑76 (MQ=255) aTAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTg < 1:1486561/76‑1 (MQ=255) aTAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTg < 1:1816340/76‑1 (MQ=255) aTAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTg < 1:1330007/76‑1 (MQ=255) aTTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACg < 1:1873758/76‑1 (MQ=255) tcttcGTTCGCCGCATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGtct < 2:55230/76‑1 (MQ=255) tcttcGTTCGCCGCATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGtct < 2:723851/76‑1 (MQ=255) cttcGTTCGCCGCATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGtctc < 2:518945/76‑1 (MQ=255) tcgttcgCCGCATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGTCTCaa > 2:65975/1‑76 (MQ=255) tcgttcgCCGCATTGCCCAATACTGCGCGGACCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGTCTCaa > 1:37502/1‑76 (MQ=255) cgttcgCCGCATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCTCATAAACACGGGTTCGTGACGTCTCAAt > 1:1002391/1‑76 (MQ=255) gttcgCCGCATTGCCCAATACTGCGCGGCCCCCGTGCTCTCGCAGCTCATCAACACGGGTTCGTGACGTCTCAATc < 2:773250/76‑1 (MQ=255) tcgCCGCATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGTCTCGATcac < 2:1351511/76‑1 (MQ=255) tcgCCGCATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGTCTCAATcac > 2:1646796/1‑76 (MQ=255) cgccgcATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGTCTCAATcacc > 2:600977/1‑76 (MQ=255) cgccgcATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGTCTCAATcacc > 2:1729609/1‑76 (MQ=255) cgccgcATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGTCTCAATcacc > 1:678202/1‑76 (MQ=255) cgccgcATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGTCTCAATcacc > 2:951192/1‑76 (MQ=255) gccgcATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGTCTCAATcacca > 1:799374/1‑76 (MQ=255) gccgcATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGCTCGTGACGTCTCAATcacca > 1:1023499/1‑76 (MQ=255) gcATTGCCCAATACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGTCGTCTCAATCaccaccg > 2:518285/1‑75 (MQ=255) ccAATTCTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGTCTCAATCACCACCAGCGGAat < 2:255949/76‑1 (MQ=255) ccAATACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGTCTCAATCACCACCAGCGGAat > 1:1060050/1‑76 (MQ=255) aaTACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCCTGACGTCTCAATCACCACCAGCGGAatat < 2:946176/76‑1 (MQ=255) aTACTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGTCTCAATCACCACCAGCGGAATATc > 2:1377828/1‑76 (MQ=255) aCTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGTCTCAATCACCACCAGCGGAATATCag > 1:401644/1‑76 (MQ=255) aCTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGTCTCAATCACCACCAGCGGAATATCag < 1:1148991/76‑1 (MQ=255) aCTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGTCTCAATCACCACCAGCGGAATATCag < 2:939216/76‑1 (MQ=255) aCTGCGCGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGTCTCAATCACCACCAGCGGAATATCag < 2:1010301/76‑1 (MQ=255) gcgcGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGTCTCAATCACCACCAGCGGAATATCAGAgg < 1:833607/76‑1 (MQ=255) gcgcGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGTCTCAATCACCACCAGCGGAATATCAGAgg > 2:1683683/1‑76 (MQ=255) gcgcGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGTCTCAATCACCACCAGCGGAATATCAGAgg < 1:1096197/76‑1 (MQ=255) gcgcGGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGTCTCAATCACCACCAGCGGAATATCAGAgg > 2:971439/1‑76 (MQ=255) cgGCCCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGTCTCAATCACCACCAGCGGAATATCAGAGGCGa > 2:112516/1‑76 (MQ=255) | GCACATTCCAGATGCGCCAGTTG‑CATAATTTCTTCGTTCGCCGCATTGCCCAATACTGCGCGGACCCCGCGCTCTCGCAGCTCATCAACACGGGTTCGTGACGTCTCAATCACCACCAGCGGAATATCAGAGGCGA > REL606/473861‑473996 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |