| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 4,163,089 | A→T | E745V (GAA→GTA) | rpoB → | DNA‑directed RNA polymerase subunit beta |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 4,163,089 | 0 | A | T | 93.2% | 272.8 / 9.7 | 74 | E745V (GAA→GTA) | rpoB | DNA‑directed RNA polymerase subunit beta |
| Reads supporting (aligned to +/- strand): ref base A (2/3); new base T (34/35); total (36/38) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.32e-01 | |||||||||||
GTGGTGTCGTTCAGTACGTGGATGCTTCCCGTATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGAAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTAACCAGAACACCTGTATCAACCAGATGC > REL606/4163017‑4163160 | gtggtgTCGTTCAGTACGTGGATGCTTCCCGTATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGTAGc > 1:861975/1‑76 (MQ=255)gtggtgTCGTTCAGTACGTGGATGCTTCCCGTATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGTAGc < 2:1402695/76‑1 (MQ=255) tggtgTCGTTCAGTACGTGGATGCTTCCCGTATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGTAGCa > 2:236908/1‑76 (MQ=255) ggtgTCGTTCAGTACGTGGATGCTTCCCGTATCGTTATCAAAGTTAACGAAGACAAGAAGTATCCGGGTGTAGCAg > 1:1119174/1‑76 (MQ=255) gtgtCGTTCAGTACGTGGATGCTTCCCGTATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGAAGCAgg < 1:1330465/76‑1 (MQ=255) tgtCGTTCAGTACGTGGATGCTTCCCGTATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGTAGCAGGt < 1:619240/76‑1 (MQ=255) tCGTTCAGTACGTGGATGCTTCGCGTATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGTAGCAGGTAt < 1:121718/76‑1 (MQ=255) tCGTTCAGTACGTGGATGCTTCCCGTATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGTAGCAGGTAt < 1:1861625/76‑1 (MQ=255) tCGTTCAGTACGTGGATGCTTCCCGTATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGAAGCAGGTAt < 1:670427/76‑1 (MQ=255) cGTTCAGTACGTGGATGCTTCCCGTATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGAAGCAGGTATc > 2:805282/1‑76 (MQ=255) gTTCAGTACGTGGATGCTTCCCGTATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGTAGCAGGTATCg < 1:912844/76‑1 (MQ=255) gTTCAGTACGTGGATGCTTCCCGTATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGTAGCAGGTATCg < 1:986388/76‑1 (MQ=255) ttCAGTACGTGGATGCTTCCCGTATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGTAGCAGGTATCGa < 2:21022/76‑1 (MQ=255) tCAGTACGTGGATGCTTCCCGTATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGTAGCAGGTATCGAc < 1:1505530/76‑1 (MQ=255) cAGTACGTGGATGCTTCCCGTATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGTAGCAGGTATCGACa > 1:564924/1‑76 (MQ=255) cAGTACGTGGATGCTTCCCGTATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGTAGCAGGTATCGACa > 2:102786/1‑76 (MQ=255) cAGTACGTGGATGCTTCCCGTATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGTAGCAGGTATCGACa > 1:1720762/1‑76 (MQ=255) aGTACGTGGATGCTTCCCGTATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGTAGCAGGTATCGACAt < 2:148106/76‑1 (MQ=255) aGTACGTGGATGCTTCCCGTATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGTAGCAGGTATCGACAt < 2:867917/76‑1 (MQ=255) aGTACGTGGATGCTTCCCGTATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGTAGCAGGTATCGACAt < 1:215255/76‑1 (MQ=255) tACGTGGATGCTTCCCGTATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGTAGCAGGTATCGACATCt < 1:210433/76‑1 (MQ=255) tACGTGGATGCTTCCCGTATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGTAGCAGGTATCGACATCt < 1:1467242/76‑1 (MQ=255) aCGTGGATGCTTCCCGTATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGTAGCAGGTATCGACATCTa > 1:763998/1‑76 (MQ=255) aCGTGGATGCTTCCCGTATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGTAGCAGGTATCGACATCTa < 2:1184547/76‑1 (MQ=255) gTGGATGCTTCCCGTATCGTTATCAAAGTTAACGACGACGAGATGTATCCGGGTGTAGCAGGTATCGACATCTACa > 2:191425/1‑76 (MQ=255) gTGGATGCTTCCCGTATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGTAGCAGGTATCGACATCTACa > 2:1586510/1‑76 (MQ=255) gTGGATGCTTCCCGTATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGTAGCAGGTATCGACATCTACa > 1:1525435/1‑76 (MQ=255) tGGATGCTTCCCGTATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGTAGCAGGTATCGACATCTACaa > 1:1661232/1‑76 (MQ=255) gATGCTTCCCGTATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGTAGCAGGTATCGACATCTACAAcc > 2:879137/1‑76 (MQ=255) gATGCTTCCCGTATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGTAGCAGGTATCGACATCTACAAcc > 1:1043668/1‑76 (MQ=255) cTTCCCGTATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGTAGCAGGTATCGACATCTACAACCTGAc > 2:1667912/1‑76 (MQ=255) tCCCGTATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGAAGCAGGTATCGACATCTACAACCTGACCa > 2:98855/1‑76 (MQ=255) ccGTATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGTAGCAGGTATCGACATCTACAACCTGACCaaa < 2:455564/76‑1 (MQ=255) ccGTATCGTTATCAAAGTTAACGAAGACGAGATGTATCCAGGTGTAGCAGGTATCGACATCTACAACCTGACCaaa < 2:455548/76‑1 (MQ=255) cGTATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGTAGCAGGTATCGACATCTACAACCTGACCAAAt > 1:104544/1‑76 (MQ=255) cGTATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGTAGCAGGTATCGACATCTACAACCTGACCAAAt < 2:460944/76‑1 (MQ=255) tATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGTAGCAGGTATCGACATCTACAACCTGACCAAATac > 1:582380/1‑76 (MQ=255) tATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGTAGCAGGTATCGACATCTACAACCTGACCAAATac > 1:47643/1‑76 (MQ=255) aTCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGTAGCAGGTATCGACATCTACAACCTGACCAAATaca < 1:596579/76‑1 (MQ=255) tCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGTAGCAGGTATCGACATCTACAACCTGACCAAATacac < 1:706537/76‑1 (MQ=255) cGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGTAGCAGGTATCGACATCTACAACCTGACCAAATACAcc < 1:399695/76‑1 (MQ=255) gTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGTAGCAGGTATCGACATCTACAACCTGACCAAATACAccc < 1:677794/76‑1 (MQ=255) ttATCAAAGTTAACGAAGACGAGATGTATCCGGGTGTAGCAGGTATCGACATCTACAACCTGACCAAATACACCCg > 2:222993/1‑76 (MQ=255) ttATCAAAGTTAACGAAGACGAGATGTATCCGGGTGTAGCAGGTATCGACATCTACAACCTGACCAAATACACCCg > 1:1070700/1‑76 (MQ=255) ttATCAAAGTTAACGAAGACGAGATGTATCCGGGTGTAGCAGGTATCGACATCTACAACCTGACCAAATACACCCg > 2:286825/1‑76 (MQ=255) ttATCAAAGTTAACGAAGACGAGATGTATCCGGGTGTAGCAGGTATCGACATCTACAACCTGACCAAATACACCCg > 1:285434/1‑76 (MQ=255) tATCAAAGTTAACGAAGACGAGATGTATCCGGGTGTAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGt > 1:158833/1‑76 (MQ=255) tATCAAAGTTAACGAAGACGAGATGTATCCGGGTGTAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGt > 1:1663799/1‑76 (MQ=255) aTCAAAGTTAACGAAGACGAGATGTATCCGGGTGTAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGtt < 2:1084357/76‑1 (MQ=255) cAAAGTTAACGAAGACGAGATGTATCCGGGTGTAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCt < 1:928641/76‑1 (MQ=255) cAAAGTTAACGAAGACGAGATGTATCCGGGTGTAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCt < 2:704022/76‑1 (MQ=255) cAAAGTTAACGAAGACGAGATGTATCCGGGTGTAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCt < 1:1213603/76‑1 (MQ=255) cAAAGTTAACGAAGACGAGATGTATCCGGGTGTAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCt > 2:1403975/1‑76 (MQ=255) cAAAGTTAACGAAGACGAGATGTATCCGGGTGTAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCt > 1:562292/1‑76 (MQ=255) aaaGTTAACGAAGACGAGATGTATCCGGGTGTAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTa > 1:1872712/1‑76 (MQ=255) aaGTTAACGAAGACGATATGTATCCGGGTGTAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTaa < 1:48076/76‑1 (MQ=255) aaGTTAACGAAGACGAGATGTATCCGGGTGTAGCAGGTATCGACATCTACAACCTGACCAACTACACCCGTTCTaa > 1:1655636/1‑76 (MQ=255) gTTAACGAAGACGAGATGTATCCGGGTGTAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTAAcc < 1:57266/76‑1 (MQ=255) ttAACGAAGACGAGATGTATCCGGGTGTAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTAACCa > 1:714743/1‑76 (MQ=255) aCGAAGACGAGATGTATCCGGGTGTAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTAACCAGaa > 1:1629447/1‑76 (MQ=255) gAAGACGAGATGTATCCGGGTGAAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTAACCAGAaca < 1:1175022/76‑1 (MQ=255) aaGACGAGATGTATCCGGGTGTAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGATCTAACCAGAacac > 1:773460/1‑76 (MQ=255) aGACGAGATGTATCCGGGTGTAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTAACCAGAACAcc > 2:745024/1‑76 (MQ=255) aCGAGATGTATCCGGGTGTAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTAACCAGAACACCTg < 2:493421/76‑1 (MQ=255) aCGAGATGTATCCGGGTGTAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTAACCAGAACACCTg < 2:1019946/76‑1 (MQ=255) gagaTGTATCCGGGTGTAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTAACCAGAACACCTGTa < 1:131566/76‑1 (MQ=255) agaTGTAGCCGGGTGTAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTAACCAGAACACCTGTAt < 1:16965/76‑1 (MQ=255) gaTGTATCCGGGTGTAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTAACCAGAACACCTGTATc < 1:1724968/76‑1 (MQ=255) tGTATCCGGGTGTAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTAACCAGAACACCTGTATCaa < 2:707725/76‑1 (MQ=255) tGTATCCGGGTGTAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTAACCAGAACACCTGTATCaa > 1:1031712/1‑76 (MQ=255) aTCCGGGTGTAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTAACCAGAACACCTGTATCAACCa > 1:911562/1‑76 (MQ=255) aTCCGGGTGTAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTAACCAGAACACCTGTATCAACCa > 2:465052/1‑76 (MQ=255) cGGGTGTAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTAACCAGAACACCTGTATCAACCAGAt < 1:396641/76‑1 (MQ=255) gggTGTAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTAACCAGAACACCTGTATCAACCAGATg < 2:762804/76‑1 (MQ=255) gggTGTAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTAACCAGAACACCTGTATCAACCAGATg < 2:303953/76‑1 (MQ=255) ggTGTAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTAACCAGAACACCTGTATCAACCAGATGc > 1:222645/1‑76 (MQ=255) | GTGGTGTCGTTCAGTACGTGGATGCTTCCCGTATCGTTATCAAAGTTAACGAAGACGAGATGTATCCGGGTGAAGCAGGTATCGACATCTACAACCTGACCAAATACACCCGTTCTAACCAGAACACCTGTATCAACCAGATGC > REL606/4163017‑4163160 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |