| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 4,164,845 | T→G | I1330M (ATT→ATG) | rpoB → | DNA‑directed RNA polymerase subunit beta |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 4,164,845 | 0 | T | G | 98.0% | 194.0 / NA | 50 | I1330M (ATT→ATG) | rpoB | DNA‑directed RNA polymerase subunit beta |
| Reads supporting (aligned to +/- strand): ref base T (1/0); major base G (27/21); minor base A (1/0); total (29/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AAAACATCGTGGACGGCAACCATCAGATGGAGCCGGGCATGCCAGAATCCTTCAACGTATTGTTGAAAGAGATTCGTTCGCTGGGTATCAACATCGAACTGGAAGACGAGTAATTCTCGCTCAAACAGGTCACTGCTGTCGGGTTA > REL606/4164772‑4164917 | aaaaCATCGTGGACGGCAACCATCAGATGGAGCCGGGCATGCCAGAATCCTTCAACGTATTGTTGAAAGAGAtgcg < 2:808733/76‑1 (MQ=255) aaCATCGTGGACGGCAACCATCAGATGGAGCCGGGCATGCCAGAATCCTTCAACGTATTGTTGAAAGAGAtgcgtt < 2:722013/76‑1 (MQ=255) cATCGTGGACGGCAACCATCAGATGGAGCCGGGCATGCCAGAATCCTTCAACGTATTGTTGAAAGAGAtgcgttcg < 1:1080000/76‑1 (MQ=255) tCGTGGACGGCAACCATCAGATGGAGCCGGGCATGCCAGAATCCTTCAACGTATTGTTGAAAGAGATGCGTTCGCt < 1:646959/76‑1 (MQ=255) tGGACGGCAACCATCAGATGGAGCCGGGCATGCCAGAATCCTTCAACGTATTGTTGAAAGAGATGCGTTCGCTggg > 1:1013351/1‑76 (MQ=255) tGGACGGCAACCATCAGATGGAGCCGGGCATGCCAGAATCCTTCAACGTATTGTTGAAAGAGATGCGTTCGCTggg > 1:1013338/1‑76 (MQ=255) ggACGGCAACCATCAGATGGAGCCGGGCATGCCAGAATCCTTCAACGTATTGTTGAAAGAGATGCGTTCGCTGGGt > 1:1081403/1‑76 (MQ=255) gACGGCAACCATCAGATGGAGCCGGGCATGCCAGAATCCTTCAACGTATTGTTGAAAGAGATGCGTTCGCTGGGTa > 2:373412/1‑76 (MQ=255) ggCAACCATCAGATGGAGCCGGGCATGCCAGAATCCTTCAACGTATTGTTGAAAGAGATGCGTTTGCTGGGTATCa > 1:114605/1‑76 (MQ=255) ggCAACCATCAGATGGAGCCGGGCATGCCAGAATCCTTCAACGTATTGTTGAAAGAGATGCGTTCGCTGGGTATCa > 2:934997/1‑76 (MQ=255) cAACCATCAGATGGAGCCGGGCATGCCAGAATCCTTCAACGTATTGTTGAAAGAGATGCGTTCGCTGGGTATCacc > 1:651299/1‑74 (MQ=255) aCCATCAGATGGAGCCGGGCATGCCAGAATCCTTCAACGTATTGTTGAAAGAGATGCGTTCGCTGGGTATCAACAt > 1:755399/1‑76 (MQ=255) ccATCAGATGGAGCCGGGCATGCCAGAATCCTTCAACGTATTGTTGAAAGAGATGCGTTCGCTGGGTATCAACATc > 1:1200709/1‑76 (MQ=255) cATCAGATGGAGCCGGGCATGCCAGAATCCTTCAACGTATTGTTGAAAGAGATGCGTTCGCTGGGTATCAACATCg > 1:480661/1‑76 (MQ=255) tCAGATGGAGCCGGGCATGCCAGAATCCTTCAACGTATTGTTGAAAGAGATGCGTTCGCTGGGTATCAACATCGaa < 2:779070/76‑1 (MQ=255) cAGATGGAGCCGGGCATGCCAGAATCCTTCAACGTATTGTTGAAAGAGATGCGTTCGCTGGGTATCAACATCGAAc > 2:1546699/1‑76 (MQ=255) aGATGGAGCCGGGCATGCCAGAATCCTTCAACGTATTGTTGAAAGAGATGCGTTCGCTGGGTATCAACATCGAACt < 2:1262246/76‑1 (MQ=255) gATGGAGCCGGGCATGCCAGAATCCTTCAACGTATTGTTGAAAGAGATGCGTTCGCTGGGTATCAACATCGAACTg > 1:446661/1‑76 (MQ=255) gATGGAGCCGGGCATGCCAGAATCCTTCAACGTATTGTTGAAAGAGATGCGTTCGCTGGGTATCAACATCGAACTg > 2:441196/1‑76 (MQ=255) tGGAGCCGGGCATGCCAGAATCCTTCAACGTATTGTTGAAAGAGATGCGTTCGCTGGGTATCAACATCGAACTGGa < 1:657162/76‑1 (MQ=255) tGGAGCCGGGCATGCCAGAATCCTTCAACGTATTGTTGAAAGAGATGCGTTCGCTGGGTATCAACATCGAACTGGa < 2:1278437/76‑1 (MQ=255) gAGCCGGGCATGCCAGAATCCTTCAACGTATTGTTGAAAGAGATACGTTCGCTGGGTATCAACATCGAACTGGAAg > 2:577364/1‑76 (MQ=255) gCCGGGCATGCCAGAATCCTTCAACGTATTGTTGAAAGAGATGCGTTCGCTGGGTATCAACATCGAACTGGAAGAc < 2:1166592/76‑1 (MQ=255) ccGGGCATGCCAGAATCCTTCAACGTATTGTTGAAAGAGATGCGTTCGCTGGGTATCAACATCGAACTGGAAGACg < 2:664645/76‑1 (MQ=255) ggCATGCCAGAATCCTTCAACGTATTGTTGAGAGAGATGCGTTCGCTGGGTATCAACATCGAACTGGAAGACGAGt < 2:1287062/76‑1 (MQ=255) gCATGCCAGAATCCTTCAACGTATTGTTGAAAGAGATGCGTTCGCTGGGTATCAACATCGAACTGGAAGACGAGTa < 1:329822/76‑1 (MQ=255) aTGCCAGAATCCTTCAACGTATTGTTGAAAGAGATTCGTTCGCTGGGTATCAACATCGAACTGGAAGACGAGCAAt > 2:102352/1‑76 (MQ=255) aTGCCAGAATCCTTCAACGTATTGTTGAAAGAGATGCGTTCGCTGGGTATCAACATCGAACTGGAAGACGAGTAAt > 1:17454/1‑76 (MQ=255) ccAGAATCCTTCAACGTATTGTTGAAAGAGATGCGTTCGCTGGGTATCAACATCGAACTGGAAGACGAGTAATtct > 1:427128/1‑76 (MQ=255) ccAGAATCCTTCAACGTATTGTTGAAAGAGATGCGTTCGCTGGGTATCAACATCGAACTGGAAGACGAGTAATtct > 2:1788137/1‑76 (MQ=255) gAATCCTTCAACGTATTGTTGAAAGAGATGCGTTCGCTGGGTATCAACATCGAACTGGAAGACGAGTAATTCTCGc > 2:1268360/1‑76 (MQ=255) aaTCCTTCAACGTATTGTTGAAAGAGATGCGTTCGCTGGGTATCGACAGCGAACGGGAAGAAGAGCACTTCgcgcg > 2:1217430/1‑75 (MQ=255) ccTTCAACGTATTGTTGAAAGAGATGCGTTCGCTGGGTATCAACATCGAACTGGAAGACGAGTAATTCTCGCTCaa > 1:1282962/1‑76 (MQ=255) cTTCAACGTATTGTTGAAAGAGATGCGTTCGCTGGGTATCAACATCGAACTGGAAGACGAGTAATTCTCGCTCaaa > 1:1599770/1‑76 (MQ=255) ttCAACGTATTGTTGAAAGAGATGCGTTCGCTGGGTATCAACATCGAACTGGAAGACGAGTAATTCTCGCTCAAAc < 2:678458/76‑1 (MQ=255) tCAACGTATTGTTGAAAGAGATGCGTTCGCTGGGTATCAACATCGAACTGGAAGACGAGTAATTCTCGCTCAAACa > 2:1118019/1‑76 (MQ=255) tCAACGTATTGTTGAAAGAGATGCGTTCGCTGGGTATCAACATCGAACTGGAAGACGAGTAATTCTCGCTCAAACa < 1:50195/76‑1 (MQ=255) cAACGTATTGTTGAAAGAGATGCGTTCGCTGGGTATCAACATCGAACTGGAAGACGAGTAATTCTCGCTCAAACAg > 1:1184478/1‑76 (MQ=255) aaCGTATTGTTGAAAGAGATGCGTTCGCTGGGTATCAACATCGAACTGGAAGACGAGTAATTCTCGCTCAAACAgg < 1:1107422/76‑1 (MQ=255) aaCGTATTGTTGAAAGAGATGCGTTCGCTGGGTATCAACATCGAACTGGAAGACGAGTAATTCTCGCTCAAACAgg < 1:162458/76‑1 (MQ=255) aaCGTATTGTTGAAAGAGATGCGTTCGCTGGGTATCAACATCGAACTGGAAGACGAGTAATTCTCGCTCAAACAgg < 1:162466/76‑1 (MQ=255) aCGTATTGTTGAAAGAGATGCGTTCGCTGGGTATCAACATCGAACTGGAAGACGAGTAATTCTCGCTCAAACAGGt < 1:1218757/76‑1 (MQ=255) gTATTGTTGAAAGAGATGCGTTCGCTGGGTATCAACATCGAACTGGAAGACGAGTAATTCTCGCTCAAACAGGTCa > 1:154597/1‑76 (MQ=255) aTTGTTGAAAGAGATGCGTTCGCTGGGTATCAACATCGAACTGGAAGACGAGTAATTCTCGCTCAAACAGGTCAct > 2:756553/1‑76 (MQ=255) tgAAAGAGATGCGTTCGCTGGGTATCAACATCGAACTGGAAGACGAGTAATTCTCGCTCAAACAGGTCACTGCTGt < 2:1364417/76‑1 (MQ=255) tgAAAGAGATGCGTTCGCTGGGTATCAACATCGAACTGGAAGACGAGTAATTCTCGCTCAAACAGGTCACTGCTGt < 1:1122569/76‑1 (MQ=255) gAAAGAGATGCGTTCGCTGGGTATCAACATCGAACTGGAAGACGAGTAATTCTCGCTCAAACAGGTCACTGCTGTc > 1:770782/1‑76 (MQ=255) aaaGAGATGCGTTCGCTGGGTATCAACATCGAACTGGAAGACGAGTAATTCTCGCTCAAACAGGTCACTGCTGTCg > 1:1121649/1‑76 (MQ=255) gagagATGCGTTCGCTGGGTATCAACATCGAACTGGAAGACGAGTAATTCTCGCTCAAACAGGTCACTGCTGTCgg > 1:990257/2‑76 (MQ=255) gagaTGCGTTCGCTGGGTATCAACATCGAACTGGAAGACGAGTAATTCTCGCTCAAACAGGTCACTGCTGTCGGGt > 2:265871/1‑76 (MQ=255) agaTGCGTTCGCTGGGTATCAACATCGAACTGGAAGACGAGTAATTCTCGCTCAAACAGGTCACTGCTGTCGGGtt < 2:427412/76‑1 (MQ=255) agaTGCGTTCGCTGGGTATCAACATCGAACTGGAAGACGAGTAATTCTCGCTCAAACAGGTCACTGCTGTCGGGtt < 1:1788088/76‑1 (MQ=255) gaTGCGTTCGCTGGGTATCAACATCGAACTGGAAGACGAGTAATTCTCGCTCAAACAGGTCACTGCTGTCGGGTTa < 2:36798/76‑1 (MQ=255) gaTGCGTTCGCTGGGTATCAACATCGAACTGGAAGACGAGTAATTCTCGCTCAAACAGGTCACTGCTGTCGGGTTa < 2:1558981/76‑1 (MQ=255) | AAAACATCGTGGACGGCAACCATCAGATGGAGCCGGGCATGCCAGAATCCTTCAACGTATTGTTGAAAGAGATTCGTTCGCTGGGTATCAACATCGAACTGGAAGACGAGTAATTCTCGCTCAAACAGGTCACTGCTGTCGGGTTA > REL606/4164772‑4164917 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |