Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	3,165,608	(A)8→7	coding (1127/2019 nt)	fadH →	2,4‑dienoyl‑CoA reductase, NADH and FMN‑linked

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	3,165,601	0	A	.	100.0%	118.9 / NA	25	coding (1120/2019 nt)	fadH	2,4‑dienoyl‑CoA reductase, NADH and FMN‑linked
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (14/11);  total (14/11)

GCAAAGTCACCTCGTGCCTGGTGAATCCTCGCGCCTGCCACGAAACCAAAATGCCAATCCTTCCCGCCGTGCAGAAAAAAAATCTGGCGGTGGTCGGTGCGGGACCTGCTGGGCTGGCGTTTGCCATTAACGCGGCGGCGCGTGG  >  REL606/3165527‑3165671                                                                           |                                                                       gCAAAGTCCCCTCGTGCCTGGTGAATCCTCGCGCCTGCCACGAAACCAAAATGCCAATCCTTCCCGCCGTGCAGaa                                                                       <  1:763206/76‑1 (MQ=255)  cAAAGTCACCTCGTGCCTGGTGAATCCTCGCGCCTGCCNCGTAACCAAAATGCCAATCCTTCCCGCCGTGCAGaaa                                                                      <  1:1720861/76‑1 (MQ=255)   aaaGTCACCTCGTGCCTGGTGAATCCTCGCGCCTGCCACGAAACCAAAATGCCAATCCTTCCCGCCGTGCAGaaaa                                                                     >  1:1055605/1‑76 (MQ=255)   aaaGTCACCTCGTGCCTGGTGAATCCTCGCGCCTGCCACGAAACCAAAATGCCAATCCTTCCCGCCGTGCAGaaaa                                                                     >  2:456970/1‑76 (MQ=255)    aaGTCCCCTCGTGCCTGGTGAATCCTCGCGCCTGCCACGAAACCAAAATGCCAATCCTTCCCGCCGTGCAGaaaaa                                                                    <  2:1542049/76‑1 (MQ=255)       tCACCTCGTGGCTGGTGTATCCTCGCGCCTGCCACGAAACCAAAATGCCAATCCTTCCCGCAGTGCAGaaaaaaat                                                                 >  1:143158/1‑75 (MQ=255)         aCCTCGTGCCTGGTGAATCCTCGCGCCTGCCACGAAACCAAAATGCCAATCCTTCCCGCCGTGCAGaaaaaaatct                                                               >  1:1279424/1‑73 (MQ=255)              gTGCCTGGTGAATCCTCGCGCCTGCCACGAAACCAAAATGCCAATCCTTCCCGCCGTGCAG‑AAAAAAATCTGGCgg                                                         >  2:1307890/1‑76 (MQ=255)                    ggTGAATCCTCGCGCCTGCCACGAAACCAAAATGCCAATCCTTCCCGCCGTGCAG‑AAAAAAATCTGGCGGTGGTCg                                                   >  1:478410/1‑76 (MQ=255)                       gAATCCTCGCGCCTGCCACGAAACCAAAATGCCAATCCTTCCCGCCGTGCAG‑AAAAAAATCTGGCGGTGGTCGGTg                                                >  2:1693155/1‑76 (MQ=255)                         aTCCTCGCGCCTGCCACGAAACCAAAATGCCAATCCTTCCCGCCGTGCAG‑AAAAAAATCTGGCGGTGGTCGGTGCg                                              >  2:1114050/1‑76 (MQ=255)                            cTCGCGCCTGCCACGAAACCAAAATGCCAATCCTTCCCGCCGTGCAG‑AAAAAAATCTGGCGGGGGTCGGTGCGGGa                                           >  1:1174737/1‑76 (MQ=255)                                 gcCTGCCACGAATCGAAAATGCCAATCCTCCCCGCCGTGCTG‑ACAAAAATCTGGCGGTGGTCGGTGCTGGACctgc                                      <  2:35184/76‑1 (MQ=255)                                 gcCTGCCACGAAACCAAAATGCCAATCCTTCCCGCCGTGCAG‑AAAAAAATCTGGCGGTGGTCGGTGCGGGACctgc                                      >  1:365140/1‑76 (MQ=255)                                    tGCCACGAAACCAAAATGCCAATCCTTCCCGCCGTGCAG‑AAAAAAATCTGGCGGTGGTCGGTGCGGGACCTgctgg                                   <  1:1322601/76‑1 (MQ=255)                                     gCCACGAAACCAAAATGCCAATCCTTCCCGCCGTGCAG‑AAAAAAATCTGGCGGTGGTCGGTGCGGGACCTgctggg                                  >  2:553317/1‑76 (MQ=255)                                      ccACGAAACCAAAACGCTACTCCTTCCCGCCGTGCAG‑AAAAAAATCTGGCGCTGGCCGGTGCGGGACCTgctgggc                                 <  1:79648/76‑1 (MQ=255)                                       cACGAAACCAAAATTCCAACGCTTCACGCGGTGCAG‑AAAAAAATCTGGCGTTGGTCGGTGCGGGACCCgctgggct                                <  2:23939/76‑1 (MQ=255)                                       cACGAAACCAAAATGCCAATCCTTCCCGCCGTGCAG‑AAAAAAATCTGGCGGGGGTCGGGGCGGGACCTgctgggcg                                >  2:679547/1‑75 (MQ=255)                                          gAAACCAAAATGCCAATCCTTCCCGCCGTGCAG‑AAAAAAATCTGGCGGTGGTCGGTGCGGGACCTGCTGGGCTGGc                             <  2:233491/76‑1 (MQ=255)                                           aaaCCAAAATGCCAATCCTTCCCGCCGTGCAG‑AAAAAAATCTGGCGGTGGTCGGTGCGGGACCTGCTGGGCTGGCg                            >  2:1350506/1‑76 (MQ=255)                                              ccAAAATGCCAATCCTTCCCGCCGTGCAG‑AAAAAAATCTGGCGGTGGTCGGTGCGGGACCTGCTGGGCTGGCGttt                         <  2:1052430/76‑1 (MQ=255)                                                  aaTGCCAATCCTTCCCGCCGTGCAG‑AAAAAAATCTGGCGGTGGTCGGTGCGGGACCTGCTGGGCTGGCGTTTGCCa                     <  2:1351521/76‑1 (MQ=255)                                                  aaTGCCAATCCTTCCCGCCGTGCAG‑AAAAAAATCTGGCGGTGGTCGGTGCGGGACCTGCTGGGCTGGCGTTTGCCa                     >  1:1388526/1‑76 (MQ=255)                                                  aaTGCCAATCCTTCCCGCCGTGCAG‑AAAAAAATCTGGCGGTGGTCGGTGCGGGACCTGCTGGGCTGGCGTTTGCCa                     <  2:657834/76‑1 (MQ=255)                                                      ccAATCCTTCCCGCCGTGCAG‑AAAAAAATCTGGCGGTGGTCGGTGCGGGACCTGCTGGGCTGGCGTTTGCCATTaa                 <  2:1344368/76‑1 (MQ=255)                                                      ccAATCCTTCCCGCCGTGCAG‑AAAAAAATCTGGCGGTGGTCGGTGCGGGACCTGCTGGGCTGGCGTTTGCCATTaa                 <  1:367696/76‑1 (MQ=255)                                                      ccAATCCTGCCCGCCGTGCAG‑AAAAAAATCTGGCGGTGGTCGGTGCGGGACCTGCTGGGCTGGCGTTTGCCATTaa                 <  1:1413778/76‑1 (MQ=255)                                                            cTTCCCGCCGTGAAG‑AAAAAAATCTGGCGGTGGACGGTGCGGGACCTGCTGGGCTGGCGTTNGCCATTAACgcggc           >  1:1517397/1‑76 (MQ=255)                                                             ttCCCGCCGTGCAG‑AAAAAAATCTGGTGGTGGTCGGTGCGGGACCTGCTGGGCTGGCGTTTGCCATTAACgcggcg          >  2:907657/1‑76 (MQ=255)                                                                 cgccgTGCAG‑AAAAAAATCTGGCGGTGGTCGGTGCGGGACCTGCTGGGCTGGCGTTTGCCATTAACGCGGCGgcgc      <  2:1613438/76‑1 (MQ=255)                                                                     gTGCAG‑AAAAAAATCTGGCGGTGGTCGGTGCGGGACCTGCTGGGCTGGCGTTTGCCATTAACGCGGCGGCGCGTgg  >  2:491258/1‑76 (MQ=255)                                                                     gTGCAG‑AAAAAAATCTGGCGGTGGTCGGTGAGGGACCTGCTGGGCTGGCGTTTGCCATTAACGCGGCGGCGCGTgg  >  2:1460163/1‑76 (MQ=255)                                                                           |                                                                       GCAAAGTCACCTCGTGCCTGGTGAATCCTCGCGCCTGCCACGAAACCAAAATGCCAATCCTTCCCGCCGTGCAGAAAAAAAATCTGGCGGTGGTCGGTGCGGGACCTGCTGGGCTGGCGTTTGCCATTAACGCGGCGGCGCGTGG  >  REL606/3165527‑3165671

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑