| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 1,004,121 | C→T | intergenic (‑47/+555) | ompF ← / ← asnS | outer membrane porin 1a (Ia;b;F)/asparaginyl‑tRNA synthetase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 1,004,121 | 0 | C | T | 92.5% | 134.1 / 0.8 | 40 | intergenic (‑47/+555) | ompF/asnS | outer membrane porin 1a (Ia;b;F)/asparaginyl‑tRNA synthetase |
| Reads supporting (aligned to +/- strand): ref base C (1/2); new base T (20/17); total (21/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.96e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ACTGCCAGAATATTGCGCTTCATCATTATTTATTACCCTCATGGTTTTTTTTATGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATC > REL606/1004049‑1004193 | ccTGCCAGAATATTGCGCTTCATCATTATTTATTCCCCTCATGTTTTTTTTTATGACCCCTGCCACTGCCGTTAAt < 2:1151670/75‑1 (MQ=255) atggcgcTTCATCATTATTTATTCCCCTCATGGTTTTTTTTAAGACCCCTGCCACTGCCGTTAATAAGTTCTGTCa < 2:950172/73‑1 (MQ=255) tGCGCTTCATCATTATTTATTACCCTCATGGTTTTTTTTATGACACCTGCCACTGCCGTTAATAAGTTCTGTCAAt > 1:187178/1‑76 (MQ=255) gcgcTTCATCATTATTTATTCCCCTCATGGTTTTTTTTATGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATa < 2:247306/76‑1 (MQ=255) gcTTCATCATTTTTTATTCCCCTCATGTTTTTTTTTATGACCCCTGCCACTGCCGTTAATAAGTTCTGTCAATaaa < 2:718225/76‑1 (MQ=255) cTTCATCATTATTTATTCCCCTCATGGTTTTTTTTATGCCCCCTGCCACTGCCGTTAATAAGTCCTGTCAATaaaa < 2:75374/76‑1 (MQ=255) ttCATCATTATTTATTCCCCTCATGTTTTTTTTTATGACACCTGCCACTGCCGTTAATCAGTTCTGTCAATaaaaa < 1:1750516/76‑1 (MQ=255) ttCATCATTATTTATTACCCTCATGGTTTTTTTTATGACACCTGCCACTGCCGTTAATAAGTTCTGTCAATaaaaa > 2:155226/1‑76 (MQ=255) attattTATTCCCCTCATGTTTTTTTTTATGACCCCTGCCACTGCCGTTAATAAGTTCTGTCAATAAAAATTTACg < 1:898210/76‑1 (MQ=255) ttatttatTACCCTCATGGTTTTTTTTATGACACCTGCCACTGCCGTTAATAAGTTCTGTCAATAAAAATTTACgg > 2:1608912/1‑76 (MQ=255) tttattACCCTCATGGTTTTTTTTATGACACCTGCCACTGCCGTTAATAAGTTCTGTCAATAAAAATTTACGGAAc > 1:810145/1‑76 (MQ=255) attaCCCTCATGGTTTTTTTTATGACACCTGCCACTGCCGTTAATAAGTTCTGTCAATAAAAATTTACGGAACTAt > 1:71054/1‑76 (MQ=255) ttaCCCTCATGGTTTTTTTTATGACACCTGCCACTGCCGTTAATAAGTTCTGTCAATAAAAATTTACGGAACTAtt > 1:666153/1‑76 (MQ=255) ccccTCATGTTTTTTTTTATGACCCCTGCCACTGCCGTTAATAAGTTCTGTCAATAAAAATTTACGGAACTATtga < 1:845503/75‑1 (MQ=255) aCCCTCATGGTTTTTTTTATGACACCTGCCACTGCCGTTAATAAGTTCTGTCAATAAAAATTTACGGAACTATtga > 2:511104/1‑76 (MQ=255) aCCCTCATGGTTTTTTTTATGACACCTGCCACTGCCGTTAATAAGTTCTGTCAATAAAAATTTACGGAACTATtga > 2:357529/1‑76 (MQ=255) ccccCATGTTTTTTTTTATGCCACCGGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATtgat < 2:524060/76‑1 (MQ=255) ccTCATGTTTTTTTTTATGCCCCCTGCCACTGCCGTTAATAAGTTCTGTCAATAAAAATTTACGGAACTATtgatg < 2:736656/76‑1 (MQ=255) tCATGTTTTTTTTTATGACCCCTGCCACTGCCGTTAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATgag < 2:623164/76‑1 (MQ=255) cATGGTTTTTTTTATGACACCTGCCACTGCCGTTAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATgaga > 2:1372153/1‑76 (MQ=255) aTGTTTTTTTTTATGACCCCTGCCACTGCCGTTAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGagag < 1:818445/76‑1 (MQ=255) gtttttttttATGCCCCCTGCCACTGCCGTTAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGtt < 2:57321/74‑1 (MQ=255) ttATGCCCCCTGCCACTGCCGTTAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCt < 2:1308646/76‑1 (MQ=255) gACACCTGCCACTGCCGTTAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTAt > 2:1653766/1‑76 (MQ=255) acCTGCGACGGCCGTCAGAAAGTTCTGTCAATAAAAATTTACGGAACTAATGATGAGAGTANGGNGTCTTTAtgtg > 2:271170/1‑76 (MQ=255) cTGCCACTGCCGTTAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTc < 1:769801/76‑1 (MQ=255) cTGCCACTGCCGTTAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTc < 1:989204/76‑1 (MQ=255) cTGCCACTGCCGTTAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTc < 1:1563758/76‑1 (MQ=255) gCCACTGCCGTTAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTg > 2:1650706/1‑76 (MQ=255) gCCACTGCCGTTAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTg > 2:591123/1‑76 (MQ=255) gCCACTGCCGTTAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTg > 2:591153/1‑76 (MQ=255) gCCACTGCCGTTAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTg > 1:575260/1‑76 (MQ=255) gCCACTGCCGTTAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTg > 2:886170/1‑76 (MQ=255) gCCACTGCCGTTAATAAGTTCTGTCAATAAAAATTTACAGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTg > 2:1650710/1‑76 (MQ=255) tGCCGTTAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGc < 2:1657069/76‑1 (MQ=255) tGCCGTTAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGc > 1:952907/1‑76 (MQ=255) tGCCGTTAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGc > 1:952884/1‑76 (MQ=255) tGCCGTTAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGc > 1:329135/1‑76 (MQ=255) ccGTTAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCAt > 2:1653267/1‑76 (MQ=255) cGTTAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATc < 2:187178/76‑1 (MQ=255) | ACTGCCAGAATATTGCGCTTCATCATTATTTATTACCCTCATGGTTTTTTTTATGACACCTGCCACTGCCGTCAATAAGTTCTGTCAATAAAAATTTACGGAACTATTGATGAGAGTTTGGTGTCTTTATGTGTCTGCAGGCATC > REL606/1004049‑1004193 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |