Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	2,031,529	G→A	N423N (AAC→AAT)	manB ←	Phosphomannomutase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	2,031,529	0	G	A	100.0%	67.2 / NA	24	N423N (AAC→AAT)	manB	Phosphomannomutase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (10/14);  total (10/14)

TAAGTTTTGTCTTTTCTTCCATCAGCGGTACATCACCGCGCGATTCCACATTCAACCGCACCACCGGTTCGGTGTTGGAGGAGCGCAGGTTAAAGCGCCAGTCGGCA  >  REL606/2031456‑2031562                                                                          |                                  tAAGTTTTGTCTTTTCTTCCATCAGCGGTACATCACCGCGCGATTCCACATTCAACCGCACCACCGGTTCGGTAtt                                 >  1:517569/1‑76 (MQ=255)   aGTTTTGTCTTTTCTTCCATCAGCGGTACATCACCGCGCGATTCCACATTCAACCGCACCACCGGTTCGGTATTgg                               <  2:52339/76‑1 (MQ=255)   aGTTTTGTCTTTTCTTCCATCAGCGGTACATCACCGCGCGATTCCACATTCAACCGCACCACCGGTTCGGTATTgg                               <  2:383533/76‑1 (MQ=255)     ttttttCTTTTCTCCCATCAGCGGTACATCCCCGCGCGATTCCACATTCAACCGCACCACCGGTTCGGTATTggag                             <  2:919307/76‑1 (MQ=17)        tGTCTTTTCTTCCATCAGCGGTACATCACCGCGCGATTCCACATTCAACCGCACCACCGGTTCGGCATTGgaggag                          >  2:1056907/1‑76 (MQ=255)        tGTCTTTTCGTCCATCAGCGGTACATCACCGCGCGATTCCACATTCAACCGCACCACCGGTTCGGTATTggaggtg                          <  1:1238376/76‑3 (MQ=14)           cTTTTCTTCCATCAGCGGTACATCACCGCGCGATTCCACATTCAACCGCACCACCGGTTCGGTATTGGAGGTgcgc                       >  1:439277/1‑76 (MQ=255)           cTTTTCTTCCATCAGCGGTACATCACCGCGCGATTCCACATTCAACCGCACCACCGGTTCGGTATTGGAGGTgcgc                       >  2:510068/1‑76 (MQ=255)           cTTTTCTTCCATCAGCGGTACATCACCGCGCGATTCCACATTCAACCGCACCACCGGTTCGGTATTGGAGGTgcgc                       >  2:455603/1‑76 (MQ=255)           cTTTTCTTCCATCAGCGGTACATCACCGCGCGATTCCACATTCAACCGCACCACCGGTTCGGTATTGGAGGTgcgc                       <  2:1105292/76‑1 (MQ=255)           cTTTTCTTCCATCAGCGGTACATCACCGCGCGATTCCACATTCAACCGCACCACCGGTTCGGTATTGGAGGTgcgc                       >  2:231553/1‑76 (MQ=255)             tttCTTCCATCAGCGGTACATCACCGCGCGATTCCACATTCAACCGCACCACCGGTTCGGTATTGGAGGTGCGCAg                     >  2:1530243/1‑76 (MQ=255)             tttCTCCCATCAGCGGTACACCCCCGCGCGATTCCACATTCAACCGCACCACCGGTTCGGTATTGGAGGTGCGCAg                     <  1:950435/76‑1 (MQ=14)              ttcttcCATCATCGGTACATCACCGCGCGATTCCACATTCAACCGCACCACCGGTTCGGTATTGGAGGTGCGCAgg                    >  1:43378/1‑76 (MQ=11)              ttcttcCATCAGCGGTACATCACCGCGCGATTCCACATTCAACCGCACCACCGGTTCGGTATTGGAGGTGCGCAgg                    >  1:655258/1‑76 (MQ=255)                cttcCATCAGCGGTACATCACCGCGCGATTCCACATTCAACCGCACCACCGGTTCGGTATTGGAGGTGCGCAGGtt                  <  1:1570369/76‑1 (MQ=255)                cttcCATCAGCGGTACATCACCGCGCGATTCCACATTCAACCGCACCACCGGTTCGGTATTGGAGGTGCGCAGGtt                  >  2:1584203/1‑76 (MQ=255)                cttcCATCAGCGGTACATCACCGCGCGATTCCACACTCAACCGCACCACCGGTTCGGTATTGGAGGTGCGCAGGtt                  >  1:359395/1‑76 (MQ=11)                  tcCATCAGCGGTACATCCCCGCGCGATTCCACATTCAACCGCACCACCGGTTCGGTATTGGAGGTGCGCAGGTTaa                <  1:1123992/76‑1 (MQ=11)                  tcCATCAGCGGTACATCACCGCGCGATTCCACATTCAACCGCACCACCGGTTCGGTATTGGAGGTGCGCAGGTTaa                <  1:636921/76‑1 (MQ=255)                  tcCATCAGCGGTACATCACCGCGCGATTCCACATTCAACCGCACCACCGGTTCGGTATTGGAGGTGCGCAGGTTaa                <  2:845780/76‑1 (MQ=255)                     aTCAGCGGTACATCACCGCGCGATTCCACATTCAACCGCACCACCGGTTCGGTATTGGAGGTGCGCATNTTAAAgc             <  2:1433434/76‑1 (MQ=11)                     aTCAGCGGTACATCACCGCGCGATTCCACATTCAACCGCACCACCGGTTCGGTATTGGAGGTGCGCAGGTTAAAgc             <  1:348727/76‑1 (MQ=255)                      tCAGCGGTACATCACCGCGCGATTCCACATTCAACCGCACCACCGGTTCGGTATTGGAGGCGCGCAGGTTAAAgcg            <  1:156940/76‑1 (MQ=255)                          cGGTACATCACCGCGCGATTCCACATTCAACCGCACCACCGGTTCGGTATTGGAGGTGCGCAGGTTAAAGCGCCAg        <  1:1567148/76‑1 (MQ=255)                                aTCACCGCGCGATTCCACATTCCACCGGCCCGCCGGTACGGTGTTGTAGGAGCGCATGTTAATGCGTCAGTCGGCa  <  1:1711268/76‑1 (MQ=17)                                                                          |                                  TAAGTTTTGTCTTTTCTTCCATCAGCGGTACATCACCGCGCGATTCCACATTCAACCGCACCACCGGTTCGGTGTTGGAGGAGCGCAGGTTAAAGCGCCAGTCGGCA  >  REL606/2031456‑2031562

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑