Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	2,193,779	G→T	L143I (CTT→ATT)	yeiB ←	conserved inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	2,193,779	0	G	T	100.0%	145.9 / NA	48	L143I (CTT→ATT)	yeiB	conserved inner membrane protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (23/25);  total (23/25)

ATGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAAGCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAGCCGCC  >  REL606/2193710‑2193850                                                                      |                                                                        aTGCATCCGGCGTCCAGGCGCGGTTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCccac                                                                   <  1:1182602/76‑1 (MQ=255)   gCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCccacca                                                                 <  2:1356606/76‑1 (MQ=255)     aTCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGa                                                               >  2:1397664/1‑76 (MQ=255)     aTCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGa                                                               >  1:488201/1‑76 (MQ=255)     aTCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGa                                                               >  2:1208785/1‑76 (MQ=255)     aTCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAAAGCCCATCCCCACCAGa                                                               >  1:206065/1‑76 (MQ=255)        cGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATaa                                                            <  1:918430/76‑1 (MQ=255)         ggCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATaaa                                                           >  1:1312054/1‑76 (MQ=255)           cGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGc                                                         >  2:490381/1‑76 (MQ=255)            gTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCa                                                        <  1:1188786/76‑1 (MQ=255)                    gcgGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCt                                                <  2:1523635/76‑1 (MQ=255)                     cgGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTg                                               <  2:812504/76‑1 (MQ=255)                      ggCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGt                                              <  2:213477/76‑1 (MQ=255)                       gCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTa                                             >  1:620305/1‑76 (MQ=255)                         tGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTAtt                                           <  1:1256292/76‑1 (MQ=255)                         tGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTAtt                                           >  1:1402772/1‑76 (MQ=255)                         tGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTAtt                                           <  1:1283019/76‑1 (MQ=255)                          ggTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTa                                          >  1:1612653/1‑76 (MQ=255)                          ggTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTa                                          <  1:1208704/76‑1 (MQ=255)                          ggTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTa                                          >  2:1414928/1‑76 (MQ=255)                           gTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTaa                                         <  1:293297/76‑1 (MQ=255)                              tGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACTCCTGTATTaaaga                                      >  2:789137/1‑74 (MQ=255)                               ggCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAg                                     <  1:1123131/76‑1 (MQ=255)                                     cGGAAATCAGCCCCAATAACAGCAAAACGCTAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCtttt                               <  2:1104978/76‑1 (MQ=255)                                      ggAAATCAGCCNCANGAACAGCAAAAAGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAAGAGCCttttt                              <  2:272141/76‑1 (MQ=255)                                      ggAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCGCCAGATAAAGCATGACGCCTGTATTAAACAGGGtgttt                              <  2:1707737/76‑1 (MQ=255)                                      ggAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCttttt                              >  1:340234/1‑76 (MQ=255)                                      ggAAATCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCttttt                              <  2:1057580/76‑1 (MQ=255)                                         aaTCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCGTTTTAcc                           >  2:416672/1‑76 (MQ=255)                                          aTCAGCCCCAATAAGAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATGAAACAGTCTTTTTACCg                          <  1:147096/76‑1 (MQ=255)                                           tCAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGa                         >  2:720711/1‑76 (MQ=255)                                            cAGCCCCAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTAccact                        >  2:216336/1‑73 (MQ=255)                                                 ccAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGgcgc                   >  2:834070/1‑76 (MQ=255)                                                 ccAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGgcgc                   >  1:765809/1‑76 (MQ=255)                                                 ccAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGgcgc                   >  1:1373402/1‑76 (MQ=255)                                                  cAATAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCa                  <  2:1505779/76‑1 (MQ=255)                                                   aaTAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCAt                 >  1:176422/1‑76 (MQ=255)                                                    aTAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATc                >  1:427705/1‑76 (MQ=255)                                                    aTAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATc                <  1:1421247/76‑1 (MQ=255)                                                    aTAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTCTACCGATGGCGCATc                >  1:1469767/1‑76 (MQ=255)                                                    aTAACAGCAAAACGCCAATCCCCACCAGATAAAGCAAGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATc                >  1:1210672/1‑76 (MQ=255)                                                     tAACAGCAAAACGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATcg               <  2:1592867/76‑1 (MQ=255)                                                       aCAGCAAAACGCCAATCCTCACAAGATNTANCTNGACGCCTNTAGTAATCAGGCTTTTTACCGATGGCGCATcgcg             <  1:1531743/76‑1 (MQ=255)                                                            aaaaCGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCa        >  2:586054/1‑76 (MQ=255)                                                            aaaaCGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCa        <  1:1677148/76‑1 (MQ=255)                                                             aaaCGCCAATCCCCACCAGAGAAAGCATGACGCCTGTATTAAACAGGCTTTTTCCCGATGGCGCATCGCGCACCAg       >  2:145125/1‑76 (MQ=255)                                                               aCGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGGTGGCGCATCGCGCACCAgcc     <  2:776717/76‑1 (MQ=255)                                                               aCGCCAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAgcc     >  1:1177674/1‑76 (MQ=255)                                                                  ccAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAgccgcc  <  2:731108/76‑1 (MQ=255)                                                                  ccAATCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAgccgcc  <  2:1169151/76‑1 (MQ=255)                                                                      |                                                                        ATGCATCCGGCGTCCAGGCGCGGCTGGTCTGGCTGTCGGAAATCAGCCCCAATAACAGCAAAACGCCAAGCCCCACCAGATAAAGCATGACGCCTGTATTAAACAGGCTTTTTACCGATGGCGCATCGCGCACCAGCCGCC  >  REL606/2193710‑2193850

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑