Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC	REL606	475,145	Δ13 bp	coding (149‑161/1677 nt)	ybaL ←	predicted transporter with NAD(P)‑binding Rossmann‑fold domain

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	REL606	= 475144	1 (0.020)	55 (1.130)	45/150	0.1	98.2%	coding (162/1677 nt)	ybaL	predicted transporter with NAD(P)‑binding Rossmann‑fold domain
	?	REL606	475158 =	1 (0.020)					coding (148/1677 nt)	ybaL	predicted transporter with NAD(P)‑binding Rossmann‑fold domain

TTCAGCGAAAAGTGCAAACCGACGCCAAACATCAACAGAATGACGCCCAGTTCAGCCAGTTCCGGGGCAAGCTTG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  REL606/475070‑475144 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑AGCCCGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGATATCCCACCAGAGGAGAAATACGTAGTTTATTGG  >  REL606/475158‑475232                                                                                                                                                        TTCAGCGAAAAGTGCAAACCGACGCCAAACATCAACAGAATGACGCCCAGTTCAGCCAGTTCCGGGGCAAGCTTGA                                                                            >  2:901009/1‑76      CGAAAAGTGCAAACCGACGCCAAACATCAACAGAATGACGCCCAGTTCAGCCAGTTCCGGGGCAAGCTTGAGCCCG                                                                       <  1:212192/76‑1        AAAAGTGCAAACCGACGCCAAACATCAACAGAATGACGCCCAGTTCAGCCAGTTCCGGGGCAAGCTTGAGCCCGGA                                                                     >  2:1057403/1‑76         AAAGTGCAAACCGACGCCAAACATCAACAGAATGACGCCCAGTTCAGCCAGTTCCGGGGCAAGCTTGAGCCCGGAG                                                                    >  2:717603/1‑76         AAAGTGCAAACCGACGCCAAACATCAACAGAATGACGCCCAGTTCAGCCAGTTCCGGGGCAAGCTTGAGCCCGGAG                                                                    >  2:774283/1‑76          AAGTGCAACCCGACGCCAAACATCAACAGAATGCCGCCCAGTTCAGCCAGTTCCGGGGCAAGCTTGAGCCCGGAGT                                                                   <  2:743422/76‑1          AAGTGCAAACCGACGCCAAACATCAACAGAATGACGCCCAGTTCAGCCAGTTCCGGGGCAAGCTTGAGCCCGGAGT                                                                   >  1:338061/1‑76           AGTGCAAACCGACGCCAAACATCAACAGAATGACGCCCAGTTCAGCCAGTTCCGGGGCAAGCTTGAGCCCGGAGTG                                                                  >  1:1074238/1‑76               CAAACCGACGCCAAACATCAACAGAATGACGCCCAGTTCAGCCAGTTCCGGGGCAAGCTTGAGCCCGGAGTGAATG                                                              >  1:1498105/1‑76                 AACCGACGCCAAACATCAACAGAATGACGCCCAGTTCAGCCAGTTCCGGGGCAAGCTTGAGCCCGGAGTGAATGGT                                                            >  2:1565734/1‑76                 AACCGACGCCAAACATCAACAGAATGACGCCCAGTTCAGCCAGTTCCGGGGCAAGCTTGAGCCCGGAGTGAATGGT                                                            <  2:1290467/76‑1                 AACCGACGCCAAACATCAACAGAATGACGCCCAGTTCAGCCAGTTCCGGGGCAAGCTTGAGCCCGGAGTGAATGGT                                                            <  2:105313/76‑1                   CCGACGCCAAACATCAACAGAATGACGCCCAGTTCAGCCAGTTCCGGGGCAAGCTTGAGCCCGGAGTGAATGGTCC                                                          >  1:1089477/1‑76                    CGACGCCAAACATCAACAGAATGACGCCCAGTTCAGCCAGTTCCGGGGCAAGCTTGAGCCCGGAGTGAATGGTCCT                                                         <  1:1160005/76‑1                     GACGCCAAACATCAACAGAATGACGCCCAGTTCAGCCAGTTCCGGGGCAAGCTTGAGCCCGGAGTGAATGGTCCTG                                                        <  2:922924/76‑1                     GACGCCAAACATCAACAGAATGACGCCCAGTTCAGCCAGTTCCGGGGCAAGCTTGAGCCCGGAGTGAATGGTCCTG                                                        <  2:1369391/76‑1                      ACGCCAAACATCAACAGAATGACGCCCAGTTCAGCCAGTTCCGGGGCAAGCTTGAGCCCGGAGTGAATGGTCCTGC                                                       <  1:123914/76‑1                      ACGCAAACCATCAACAGAAGGACGCCCAGTTCAGCCAGTTCCGGGGCAAGCTTGAGCCCGGAGTGAATGGTCCCGC                                                       <  1:123899/76‑1                        GCCAAACATCAACAGAATGACGCCCAGTTCAGCCAGTTCCGGGGCAAGCTTGAGCCCGGAGTGAATGGTCCTGCCA                                                     <  1:1320258/76‑1                         CCAAACATCAACAGAATGACGCCCAGTTCAGCCAGTTCCGGGGCAAGCTTGAGCCCGGAGTGAATGGTCCTGCCAG                                                    <  2:686279/76‑1                          CAAACATCAACAGAATGACGCCCAGTTCAGCCAGTTCCGGGGCAAGCTTGAGCCCGGAGTGAATGGTCCTGCCAGC                                                   >  2:784154/1‑76                           AAACATCAACAGAATGACGCCCAGTTCAGCCAGTTCCGGGGCAAGCTTGAGCCCGGAGTGAATGGTCCTGCCAGCA                                                  >  2:1347364/1‑76                              CATCAACAGAATGACGCCCAGTTCAGCCAGTTCCGGGGCAAGCTTGAGCCCGGAGTGAATGGTCCTGCCAGCACAC                                               >  1:117007/1‑76                               ATCAACAGAATGACGCCCAGTTCAGCCAGTTCCGGGGCAAGCTTGAGCCCGGAGTGAATGGTCCTGCCAGCACACC                                              >  2:983802/1‑76                                 CAACAGAATGACGCCCAGTTCAGCCAGTTCCGGGGCAAGCTTGAGCCCGGAGTGAATGGTCCTGCCAGCACACCCG                                            >  1:62102/1‑76                                     AGAATGACGCCCAGTTCAGCCAGTTCCGGGGCACGCTTGAGCCCGGAGTGAATGGTCCTGCCAGCACACCCGCTAA                                        >  2:722731/1‑76                                      GAATGACGCCCAGTTCAGCCAGTTCCGGGGCAAGCTTGAGCCCGGAGTGAATGGTCCTGCCAGCACACCCGCTAAC                                       <  2:1051468/76‑1                                         TGACGCCCAGTTCAGCCAGTTCCGGGGCAAGCTTGAGCCCGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGA                                    >  2:672684/1‑76                                         TGACGCCCAGTTCAGCCAGTTCCGGGGCAAGCTTGAGCCCGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGA                                    >  2:235538/1‑76                                         TGACGCCCAGTTCAGCCAGTTCCGGGGCAAGCTTGAGCCCGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGA                                    >  2:1401839/1‑76                                          GACGCCCAGTTCAGCCAGTTCCGGGGCAAGCTTGAGCCCGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGAT                                   <  1:1020727/76‑1                                          GACGCCCAGTTCAGCCAGTTCCGGGGCAAGCTTGAGCCCGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGAT                                   <  1:536325/76‑1                                           ACGCCCAGTTCAGCCAGTTCCGGGGCAAGCTTGAGCCCGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGATA                                  >  2:411751/1‑76                                             GCCCAGTTCAGCCAGTTCCGGGGCAAGCTTGAGCCCGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGATATC                                <  2:1066186/76‑1                                                 AGTTCAGCCAGTTCCGGGGCAAGCTTGAGCCCGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGATATCCCAC                            <  2:333804/76‑1                                                 AGTTCAGCCAGTTCCGGGGCAAGCTTGAGCCCGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGATATCCCAC                            >  1:1375572/1‑76                                                   TTCAGCCAGTTCCGGGGCAAGCTTGAGCCCGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGATATCCCACCA                          <  1:777450/76‑1                                                     CAGTCAGTTCCGGGGCAAGCTTGAGCCCGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGATATCCCACCAGA                        <  2:769947/76‑1                                                     CAGCCAGTTCCGGGGCAAGCTTGAGCCCGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGATATCCCACCAGA                        >  1:129449/1‑76                                                      AGCCAGTTCCGGGGCAAGCTTGAGCCCGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGATATCCCACCAGAG                       <  1:710659/76‑1                                                         CAGTTCCGGGGCAAGCTTGAGCCCGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGATATCCCACCAGAGGAG                    <  1:1507024/76‑1                                                          AGTTCCGGGGCAAGCTTGAGCCCGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGATATCCCACCAGAGGAGA                   <  1:656109/76‑1                                                          AGTTCCGGGGCAAGCTTGAGCCCGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGATATCCCACCAGAGGAGA                   >  1:823031/1‑76                                                            TTCCGGGGCAAGCTTGAGCCCGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGATATCCCACCAGAGGAGAAA                 >  2:1009568/1‑76                                                             TCCGGGGCAAGCTTGAGCCCGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGATATCCCACCAGAGGAGAAAT                <  2:1291657/76‑1                                                             TCCGGGGCAAGCTTGAGCCCGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGATATCCCACCAGAGGAGAAAT                <  1:1229672/76‑1                                                             TCCGGGGCAAGCTTGAGCCCGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGATATCCCACCAGAGGAGAAAT                <  1:1569715/76‑1                                                             TCCGGGGCAAGCTTGAGCCCGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGATATCCCACCAGAGGAGAAAT                <  1:660909/76‑1                                                              CCGGGGCAAGCTTGAGCCCGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGATATCCCACCAGAGGAGAAATA               >  1:422959/1‑76                                                               CGGGGCAAGCTTGAGCCCGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGATATCCCACCAGAGGAGAAATAC              >  2:787808/1‑76                                                                GGGGCAAGCTTGAGCCCGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGATATCCCACCAGAGGAGAAATACG             >  2:1015952/1‑76                                                                     AAGCTTGAGCCCGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGATATCCCACCAGAGGAGAAATACGTAGTT        >  2:944766/1‑76                                                                       GCTTGAGCCCGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGATATCCCACCAGAGGAGAAATACGTAGTTTA      >  2:755465/1‑76                                                                          TGAGCCCGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGATATCCCACCAGAGGAGAAATACGTAGTTTATTG   >  1:938659/1‑76                                                                           GAGCCCGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGATATCCCACCAGAGGAGAAATACGTAGTTTATTGG  <  1:511424/76‑1                                                                                                                                                        TTCAGCGAAAAGTGCAAACCGACGCCAAACATCAACAGAATGACGCCCAGTTCAGCCAGTTCCGGGGCAAGCTTG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  REL606/475070‑475144 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑AGCCCGGAGTGAATGGTCCTGCCAGCACACCCGCTAACAGATATCCCACCAGAGGAGAAATACGTAGTTTATTGG  >  REL606/475158‑475232

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.