| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 2,448,098 | 0 | T | G | 34.9% | 73.7 / 13.0 | 43 | F278V (TTT→GTT) | nupC | nucleoside (except guanosine) transporter |
| Reads supporting (aligned to +/- strand): ref base T (4/24); new base G (15/0); total (19/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.56e-08 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TGCCGCGATGCTGATCGGCTTTATCGCCCTGATCGCCGCGCTGAACGCACTGTTTGCCACCGTTACTGGCTGGTTTGGCTACAGCATCTCCTTCCAGGGCATCCTGGGCTACATCTTCTATCCGATTGCATGGGTGATGGGTGTTCCT > REL606/2448025‑2448172 | tGCCGCGATGCTGATCGGCTTTATCGCCCTGATCGCCGCGCTGAACGCACTGTTTGCCACCGTTACTGGCTGGttt < 2:165331/76‑1 (MQ=255) cgATGCTGATCGGCTTTATCGCCCTGATCGCCGCGCTGAACGCACTGTTTGCCACCGTTACTGGCTGGTTTGGCTa < 1:1469958/76‑1 (MQ=255) gATGCTGATCGGCTTTATCGCCCTGATCGCCGCGCTGAACGCACTGTTTGCCACCGTTACTGGCTGGGTTGGGTAc > 1:478939/1‑76 (MQ=255) gATGCTGATCGGCTTTATCGCCCTGATCGCCGCGCTGAACGCACTGTTTGCCACCGTTACTGGCTGGGTTGGCTAc > 2:1133272/1‑76 (MQ=255) aTGCTGATCGGCTTTATCGCCCTGATCGCCGCGCTGAACGCACTGTTTGCCACCGTTACTGGCTGGTTTGGCTACa < 1:734234/76‑1 (MQ=255) aTGCTGATCGGCTTTATCGCCCTGATCGCCGCGCTGAACGCACTGTTTGCCACCGTTACTGGCTGGGTTGGCTACa > 1:104344/1‑76 (MQ=255) gCTGATCGGCTTTATCGCCCTGATGGCCGCGCTGAACGCACTGTTTGCCACCGTTACTGGCTGGGTTGGGTACAGc > 1:1016326/1‑76 (MQ=255) cTGATCGGCTTTATCGCCCTGATCGCCGCGCTGAACGCACTGTTTGCCACCGTTACTGGCTGGGTTGGCTACAGCa > 2:1256109/1‑76 (MQ=255) cTGATCGGCTTTATCGCCCTGATCGCCGCGCTGAACGCACTGTTTGCCACCGTTACTGGCTGGGTTGGCTACAGCa > 2:1249632/1‑76 (MQ=255) tGATCGGCTTTATCGCCCTGATCGCCGCGCTGAACGCACTGTTTGCCACCGTTACTGGCTGGTTTGGCTACAGCAt < 1:459156/76‑1 (MQ=255) gATCGGCTTTATCGCCCTGATCGCCGCGCTGAACGCACTGTTTGCCACCGTTACTGGCTGGTTTGGCTACAGCAtc < 1:321772/76‑1 (MQ=255) gATCGGCTTTATCGCCCTGATCGCCGCGCTGAACGCACTGTTTGCCACCGTTACTGGCTGGGTTTGGTACCGCAtc > 1:230849/1‑76 (MQ=255) gATCGGCTTTATCGCCCTGATCGCCGCGCTGAACGCACTGTTTGCCACCGTTACTGGCTGGGTTGGCTACCGccac > 2:1356516/1‑73 (MQ=255) gATCGGCTTTATCGCCCTGATCGCCGCGCTGAACGCACTGTTTGCCACCGTTACTGGCTGGGTTGGCTACAGCAtc > 1:1099896/1‑76 (MQ=255) aTCGGCTTTATCGCCCTGATCGCCGCGCTGAACGCACTGTTTGCCACCGTTACTGGCTGGTTTGGCTACAGCAtct < 2:46584/76‑1 (MQ=255) gCTTTATCGCCCTGATCGCCGCGCTGAACGCACTGTTTGCCACCGTTACTGGCTGGTTTGGCTACAGCATCTCCtt < 2:1113696/76‑1 (MQ=255) ttATCGCCCTGATCGCCGCGCTGAACGCACTGTTTGCCACCGTTACTGGCTGGGTTGGGTACAGCATGTCCCTccc > 2:23498/1‑75 (MQ=255) cGCCCTGATCGCCGCGCTGAACGCACTGTTTGCCACCGTTACTGGCTGGTTTGGCTACAGCATCTCCTTCCAGGGc < 2:402772/76‑1 (MQ=255) ccTGATCGCCGCGCTGAACGCACTGTTTGCCACCGTTACTGGCTGGTTTGGCTACAGCATCTCCTTCCAGGGCATc < 2:1376401/76‑1 (MQ=255) gATCGCCGCGCTGAACGCACTGTTTGCCACCGTTACTGGCTGGTTTGGCTACAGCATCTCCTTCCAGGGCATCCTg < 1:1060918/76‑1 (MQ=255) gATCGCCGCGCTGAACGCACTGTTTGCCACCGTTACTGGCTGGTTTGGCTACAGCATCTCCTTCCAGGGCATCCTg < 2:786076/76‑1 (MQ=255) gATCGCCGCGCTGAACGCACTGTTTGCCACCGTTACTGGCTGGGTTGGCTACAGCATCTCCTTCCCGGGCATcccg > 1:172690/1‑74 (MQ=255) gATCGCCGCGCTGAACGCACTGTTTGCCACCGTTACTGGCTGGGTTGGCTACAGCATCTCCCTCCCGGGGATcccg > 2:521813/1‑74 (MQ=255) aTCGCCGCGCTGAACGCACTGTTTGCCACCGTTACTGGCTGGTTTGGCTACAGCATCTCCTTCCAGGGCATCCTgg < 2:960136/76‑1 (MQ=255) tCGCCGCGCTGAACGCACTGTTTGCCACCGTTACTGGCTGGTTTGGCTACAGCATCTCCTTCCAGGGCATCCTggg < 2:917835/76‑1 (MQ=255) cgcTGAACGCACTGTTTGCCACCGTTACTGGCTGGTTTGGCTACAGCATCTCCTTCCAGGGCATCCTGGGCTACAt < 2:966429/76‑1 (MQ=255) cgcTGAACGCACTGTTTGCCACCGTTACTGGCTGGTTTGGCTACAGCATCTCCTTCCAGGGCATCCTGGGCTACAt < 2:165205/76‑1 (MQ=255) cgcTGAACGCACTGTTTGCCACCGTTACTGGCTGGGTTGGCTACAGCATCTCCTTCCCGGGCATCCTGGGCTACAt > 1:1049211/1‑76 (MQ=255) aaCGCACTGTTTGCCACCGTTACTGGCTGGGTTTGCTACAGCATCTCCCTCCAGGGGCTTCTGGGGCACCtctttt > 2:1210446/1‑74 (MQ=255) cGCACTGTTTGCCACCGTTACTGGCTGGTTTGGCTACAGCATCTCCTTCCAGGGCATCCTGGGCTACATCTTCTAt < 2:548447/76‑1 (MQ=255) gCACTGTTTGCCACCGTTACTGGCTGGTTTGGCTACAGCATCTCCTTCCAGGGCATCCTGGGCTACATCTTCTATc < 2:1486849/76‑1 (MQ=255) aCTGTTTGCCACCGTTACTGGCTGGTTTGGCTACAGCATCTCCTTCCAGGGCATCCTGGGCTACATCTTCTATCCg < 1:1398257/76‑1 (MQ=255) aCTGTTTGCCACCGTTACTGGCTGGTTTGGCTACAGCATCTCCTTCCAGGGCATCCTGGGCTACATCTTCTATCCg < 1:1484833/76‑1 (MQ=255) aCTGTTTGCCACCGTTACTGGCTGGTTTGGCTACAGCATCTCCTTCCAGGGCATCCTGGGCTACATCTTCTATCCg < 1:315216/76‑1 (MQ=255) gTTTGCCACCGTTACTGGCTGGTTTGGCTACCGCCACTCCTTCCACGGGATTCTGGGGTGCATCTTCTATCCCAtt > 2:1535708/1‑76 (MQ=255) tttGCCACCGTTACTGGCTGGTTTGGCTACAGCATCTCCTTCCAGGGCATCCTGGGCTACATCTTCTATCCGATTg < 2:423387/76‑1 (MQ=255) tttGCCACCGTTACTGGCTGGGTTTGCTACAGCATCTCCTTCCCGGGCATCCTGGGCTACATCTTCTATCCGATTg > 2:1194528/1‑76 (MQ=255) tttGCCACCGTTACTGGCTGGGTTGGCTACAGCATCTCCTTCCCGGGCATCCTGGGCTACATCTTCTATCCGATTg > 1:757328/1‑76 (MQ=255) tttGCCACCGTTACTGGCTGGGTTGGCTACAGCATCTCCTTCCCGGGCATCCTGGGCTACATCTTCTATCCCATTg > 1:847967/1‑76 (MQ=255) tGCCACCGTTACTGGCTGGTTTGGCTACAGCATCTCCTTCCAGGGCATCCTGGGCTACATCTTCTATCCGATTGCa < 2:1270198/76‑1 (MQ=255) cACCGTTACTGGCTGGTTTGGCTTCCGCATCTCCTTCCCGGGCCGCCCGGGGTACCTCTTCTTTCCCCATGCatgg > 2:1150565/1‑76 (MQ=255) cACCGTTACTGGCTGGTTTGGCTACAGCATCTCCTTCCAGGGCATCCTGGGCTACATCTTCTATCCGATTGCatgg > 2:1117691/1‑76 (MQ=255) aCCGTTACTGGCTGGTTTGGCTACAGCATCTCCTTCCAGGGCATCCTGGGCTACATCTTCTTTCCGATTGCatggg > 2:805610/1‑76 (MQ=255) aCCGTTACTGGCTGGGTTGGGTACAGCATCTCCTTCCCGGGCATCCTGGGGCACATCTTCTATCCGCTTTCatggg > 2:1112992/1‑76 (MQ=255) cGTTACTGGCTGGTTTGGCTACAGCATCTCCTTCCAGGGCATCCTGGGCTACATCTTCTATCCGATTGCatgggtg < 2:569029/76‑1 (MQ=255) cGTTACTGGCTGGGTTTGCTACAGCATCTCCTTCCAGGGCCTCCTGGGCCACATCTTCTTTCCGATTTCctggggg > 2:665687/1‑74 (MQ=255) tACTGGCTGGTTTGGCTACAGCATCTCCTTCCAGGGCATCCTGGGCTACATCTTCTATCCGATTGCatgggtgatg < 2:474919/76‑1 (MQ=255) aCTGGCTGGGTTGGCTACAGCATCTCCTTCCCGGGCATCCTGGGCTACATCTTCTATCCGATTGCatgggtggtgg > 2:1169292/1‑76 (MQ=255) gTTTGGCTACAGCATCTCCTTCCAGGGCATCCTGGGCTACATCTTCTATCCGATTGCATGGGTGATGGGTGttcct < 2:1315897/76‑1 (MQ=255) | TGCCGCGATGCTGATCGGCTTTATCGCCCTGATCGCCGCGCTGAACGCACTGTTTGCCACCGTTACTGGCTGGTTTGGCTACAGCATCTCCTTCCAGGGCATCCTGGGCTACATCTTCTATCCGATTGCATGGGTGATGGGTGTTCCT > REL606/2448025‑2448172 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |