Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*REL6063,492,0820TG39.1% 72.2 / 18.9 46V490G (GTG→GGG) glpDsn‑glycerol‑3‑phosphate dehydrogenase, aerobic, FAD/NAD(P)‑binding
Reads supporting (aligned to +/- strand):  ref base T (4/24);  new base G (18/0);  total (22/24)
Fisher's exact test for biased strand distribution p-value = 2.59e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

CCCTGTGGCGTCGCACAAAACAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGAAGCTGTCGCTGGCGTCGTGATTTAATGTAAGGTGGCCAGGTCAGATTTCAATCTGACC  >  REL606/3492010‑3492157
                                                                        |                                                                           
cccTGTGGCGTCGCACAAAACAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCtgggggg                                                                          >  2:1452399/1‑75 (MQ=255)
 ccTGTGGCGTCGCACAAAACAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAg                                                                         <  1:171599/76‑1 (MQ=255)
    gtgGCGTCGCACAAAACAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGtat                                                                      <  1:1050298/76‑1 (MQ=255)
     tgGCGTCGCACAAAACAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGtata                                                                     <  1:1382022/76‑1 (MQ=255)
      ggCGTCGCACAAAACAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGGGGGGTATAc                                                                    >  2:1046121/1‑76 (MQ=255)
      ggCGTCGCACAAAACAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGGGGGGGATAc                                                                    >  1:1202032/1‑76 (MQ=255)
         gTCGCACAAAACAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGGGGGGGATAAgca                                                                 >  1:229932/1‑76 (MQ=255)
          tCGCACAAAACAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGTATACgcag                                                                <  1:620340/76‑1 (MQ=255)
          tCGCACAAAACAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGTATACgcag                                                                <  2:153396/76‑1 (MQ=255)
          tCGCACAAAACAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGGGGAGGATACgcag                                                                >  1:556162/1‑76 (MQ=255)
            gCACAAAACAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGTATACgcagca                                                              <  1:1253170/76‑1 (MQ=255)
             cacaAAACAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGGGGGGGATACGccgcag                                                             >  1:820373/1‑76 (MQ=255)
              acaAAACAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGGGGGGTATAAGCAGCCGa                                                            >  2:797901/1‑76 (MQ=255)
               caAAACAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGaa                                                           <  1:1456559/76‑1 (MQ=255)
                  aaCAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGGGGGGGATACGCAGCAGAAGCt                                                        >  2:536934/1‑76 (MQ=255)
                   aCAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGAAGCTg                                                       <  2:1424684/76‑1 (MQ=255)
                     aaGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGGGGAGTATACGCAGCAGAAGCTGTc                                                     >  2:388905/1‑76 (MQ=255)
                        gCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGAAGCTGTCGCt                                                  <  1:310292/76‑1 (MQ=255)
                         cATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGAAGCTGTCGCTg                                                 <  2:1390703/76‑1 (MQ=255)
                            gtgGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGGGGGGTATACGCAGCAGAAGctggcgctggcg                                              >  1:1116038/1‑76 (MQ=255)
                             tgGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGGGGGGGATACGCAGCAGAAGCTGTCGCTGGcgg                                             >  2:112937/1‑75 (MQ=255)
                              ggCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGGGGGGTATACGCAGCAGAAGCTGTCGCTGGcggc                                            >  1:1388069/1‑74 (MQ=255)
                                cTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGGGGATACGCAGCAGAAGCTGTCGCTTGcgtcgt                                          >  1:1359523/1‑76 (MQ=255)
                                cTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGGGGAGTATACGCAGCAGAAGCTGTCGCTGGcgtcgg                                          >  1:220228/1‑75 (MQ=255)
                                 tAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGGGGGGTATACGCAGCAGAAGCTGTCGCTGGGGGCGTg                                         >  2:1193225/1‑76 (MQ=255)
                                  aaaTGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGGGGAGTATACGCAGCAGAAGCTGTCGCTGGCGTCGTGa                                        >  2:105533/1‑76 (MQ=255)
                                   aaTGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGAAGCTGTCGCTGGCGTCGTGAt                                       <  2:159531/76‑1 (MQ=255)
                                     tGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGGGGAGTATACGCAGCAGAAGCTGTCGCTGGGGTCGTGGttt                                     >  1:732804/1‑76 (MQ=255)
                                       cGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGAAGCTGTCGCTGGCGTCGTGATTTaa                                   <  2:1048930/76‑1 (MQ=255)
                                       cGGATCAACAATCTCGTGTGAGTCAGTGGCTGGGGGGGGATACGCAGCAGAAGCTGTCGCTGGCGGCGGGGtttta                                   >  2:1023956/1‑74 (MQ=255)
                                       cGGATCAACAATCTCGTGTGAGTCAGTGGCTGGGGGAGTATACGCAGCAGAAGCTGTCGCTGGGGGCGGGATTTaa                                   >  2:236898/1‑76 (MQ=255)
                                        ggATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGAAGCTGTCGCTGGCGTCGTGATTTAAt                                  <  2:969912/76‑1 (MQ=255)
                                          aTCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGAAGCTGTCGCTGGCGTCGTGATTTAATGt                                <  1:11629/76‑1 (MQ=255)
                                           tCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGAAGCTGTCGCTGGCGTCGTGATTTAATGTa                               <  1:688025/76‑1 (MQ=255)
                                             aacaaTCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGAAGCTGTCGCTGGCGTCGTGATTTAATGTAAg                             <  1:1308699/76‑1 (MQ=255)
                                             aacaaTCTCGTGTGAGTCAGTGGCTGGGGGGGGATACGCAGCAGAAGCTGTCGCTGGGGTCGTGGTTTAATGTAAg                             >  2:1410588/1‑76 (MQ=255)
                                                aaTCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGAAGCTGTCGCTGGCGTCGTGATTTAATGTAAGGTg                          >  1:927210/1‑76 (MQ=255)
                                                 aTCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGAAGCTGTCGCTGGCGTCGTGATTTAATGTAAGGTgg                         <  2:99703/76‑1 (MQ=255)
                                                   ctcGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGAAGCTGTCGCTGGCGTCGTGATTTAATGTAAGGTGGcc                       <  2:1276202/76‑1 (MQ=255)
                                                     cGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGAAGCTGTCGCTGGCGTCGTGATTTAATGTAAGGTGGCCAg                     <  1:126318/76‑1 (MQ=255)
                                                        gtgAGTCAGTGGCTGGTGGAGTATACGCAGCAGAAGCTGTCGCTGGCGTCGTGATTTAATGTAAGGTGGCCAGGTc                  <  1:662578/76‑1 (MQ=255)
                                                        gtgAGTCAGTGGCTGGTGGAGTATACGCAGCAGAAGCTGTCGCTGGCGTCGTGATTTAATGTAAGGTGGCCAGGTc                  <  2:632780/76‑1 (MQ=255)
                                                        gtgAGTCAGTGGCTGGTGGAGTATACGCAGCAGAAGCTGTCGCTGGCGTCGTGATTTAATGTAAGGTGGCCAGGTc                  <  2:864994/76‑1 (MQ=255)
                                                            gTCAGTGGCGGGTGGAGTATACGCAGCAGAAGCTGTCGCTGGCGTCGTGATTTAATGTAAGGTGGCCAGGTCAGAt              <  1:908455/76‑1 (MQ=255)
                                                              cAGTGGCTGGTGGAGTATACGCAGCAGAAGCTGTCGCTGGCGTCGTGATTTAATGTAAGGTGGCCAGGTCAGAttt            <  2:1125800/76‑1 (MQ=255)
                                                              cAGTGGCTGGTGGAGTATACGCAGCAGAAGCTGTCGCTGGCGTCGTGATTTAATGTAAGGTGGCCAGGTCAGAttt            <  1:710556/76‑1 (MQ=255)
                                                                 tGGCTGGTGGAGTATACGCAGCAGAAGCTGTCGCTGGCGTCGTGATTTAATGTAAGGTGGCCAGGTCAGATTTCaa         >  2:648799/1‑76 (MQ=255)
                                                                  ggCTGGTGGAGTATACGCAGCAGAAGCTGTCGCTGGCGTCGTGATTTAATGTAAGGTGGCCAGGCCAGGTTTCAAt        >  1:942190/1‑76 (MQ=255)
                                                                    cTGGTGGAGTATACGCAGCAGAAGCTGTCGCTGGCGTCGTGATTTAATGTAAGGTGGCCAGGTCAGATTTCAATCt      >  2:165294/1‑76 (MQ=255)
                                                                      ggtggAGTATACGCAGCAGAAGCTGTCGCTGGCGTCGTGATTTAATGTAAGGTGGCCAGGTCAGATTTCAATCTGa    <  1:867907/76‑1 (MQ=255)
                                                                      ggtggAGTATACGCAGCAGAAGCTGTCGCTGGCGTCGTGATTTAATGTAAGGTGGCCAGGTCAGATTTCAATCTGa    <  1:740649/76‑1 (MQ=255)
                                                                        tggAGTATACGCAGCAGAAGCTGTCGCTGGCGTCGTGATTTAATGTAAGGTGGCCAGGTCGGATTTCAATCTGAcc  >  1:135289/1‑76 (MQ=255)
                                                                        tggAGTATACGCAGCAGAAGCTGTCGCTGGCGTCGTGATTTAATGTAAGGTGGCCAGGTCAGATTTCAATCTGAcc  >  2:1367784/1‑76 (MQ=255)
                                                                        |                                                                           
CCCTGTGGCGTCGCACAAAACAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGAAGCTGTCGCTGGCGTCGTGATTTAATGTAAGGTGGCCAGGTCAGATTTCAATCTGACC  >  REL606/3492010‑3492157

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑