| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 305,821 | T→G | Y268D (TAT→GAT) | yahA → | predicted DNA‑binding transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 305,821 | 0 | T | G | 97.1% | 137.8 / ‑2.7 | 34 | Y268D (TAT→GAT) | yahA | predicted DNA‑binding transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base T (0/1); new base G (12/21); total (12/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.25e-01 | |||||||||||
CAGAGCGATATTTGACAGCCTTCATCAGCACAACATTACCTTTGCGCTGGATGACTTTGGTACGGGTTATGCGACCTATCGTTACTTGCAGGCGTTCCCGGTCGATTTTATTAAGATCGATAAGTCATTTGTGCAAA > REL606/305754‑305890 | cAGAGCGATATTTGACAGCCTTCATCAGCACAACATTACCTTTGCGCTGGATGACTTTGGTACGGGTGATGCGAcc > 2:12692/1‑76 (MQ=255)cAGAGCGATATTTGACAGCCTTCATCAGCACAACATTACCTTTGCGCTGGATGACTTTGGTACGGGTGATGCGAcc > 2:1242079/1‑76 (MQ=255)cAGAGCGATATTTGACAGCCTTCATCAGCACAACATTACCTTTGCGCTGGATGACTTTGGTACGGGTGATGCGAcc > 2:867725/1‑76 (MQ=255) agCGATATTTGACAGCCTTCATCAGCACAACATTACCTTTGCGCTGGATGACTTTGGTACGGGTGATGCGACCTAt < 2:1185437/76‑1 (MQ=255) agCGATATTTGACAGCCTTCATCAGCACAACATTACCTTTGCGCTGGATGACTTTGGTACGGGTGATGCGACCTAt < 2:514180/76‑1 (MQ=255) agCGATATTTGACAGCCTTCATCAGCACAACATTACCTTTGCGCTGGATGACTTTGGTACGGGTGATGCGACCTAt < 1:743034/76‑1 (MQ=255) gATATTTGACAGCCTTCATCAGCACAACATTACCTTTGCGCTGGATGACTTTGGTACGGGTGATGCGACCTATCGt < 1:143349/76‑1 (MQ=255) tatTTGACAGCCTTCATCAGCACAACATTACCTTTGCGCTGGATGACTTTGGTACGGGTGATGCGACCTATCGTTa > 2:1089838/1‑76 (MQ=255) tttGACAGCCTTCATCAGCACAACATTACCTTTGCGCTGGATGACTTTGGTACGGGTGATGCGACCTATCGTTACt > 2:1497570/1‑76 (MQ=255) tGACAGCCTTCATCAGCACAACATTACCTTTGCGCTGGATGACTTTGGTACGGGTGATGCGACCTATCGTTACTTg > 1:328590/1‑76 (MQ=255) aCAGCCTTCATCAGCACAACATTACCTTTGCGCTGGATGACTTTGGTACGGGTGATGCGACCTATCGTTACTTGCa < 1:434769/76‑1 (MQ=255) cAGCCTTCATCAGCACAACATTACCTTTGCGCTGGATGACTTTGGTACGGGTGATGCGACCTATCGTTACTTGCAg < 2:389031/76‑1 (MQ=255) cAGCCTTCATCAGCACAACATTACCTTTGCGCTGGATGACTTTGGTACGGGTGATGCGACCTATCGTTACTTGCAg > 2:100095/1‑76 (MQ=255) aGCCTTCATCAGCACAACATTACCTTTGCGCTGGATGACTTTGGTACGGGTGATGCGACCTATCGTTACTTGCAgg < 1:1202061/76‑1 (MQ=255) aGCCTTCATCAGCACAACATTACCTTTGCGCTGGATGACTTTGGTACGGGTGATGCGACCTATCGTTACTTGCAgg < 2:1299901/76‑1 (MQ=255) ccTTCATCAGCACAACATTACCTTTGCGCTGGATGACTTTGGTACGGGTGATGCGACCTATCGTTACTTGCAGGCg < 1:1408076/76‑1 (MQ=255) ccTTCATCAGCACAACATTACCTTTGCGCTGGATGACTTTGGTACGGGTGATGCGACCTATCGTTACTTGCAGGCg > 2:19076/1‑76 (MQ=255) cTTCATCAGCACAACATTACCTTTGCGCTGGATGACTTTGGTACGGGTGATGCGACCTATCGTTACTTGCAGGCGt > 2:428713/1‑76 (MQ=255) ttCATCAGCACAACATTACCTTTGCGCTGGATGACTTTGGTACGGGTGATGCGACCTATCGTTACTTGCAGGCGtt < 2:16781/76‑1 (MQ=255) atcaGCACAACATTACCTTTGCGCTGGATGACTTTGGTACGGGTGATGCGACCTATCGTTACTTGCAGGCGTTccc < 2:332236/76‑1 (MQ=255) tcaGCACAACATTACCTTTGCGCTGGATGACTTTGGTACGGGTGATGCGACCTATCGTTACTTGCAGGCGTTCCCg < 2:673304/76‑1 (MQ=255) aacaTTACCTTTGCGCTGGATGACTTTGGTACGGGTGATGCGACCTATCGTTACTTGCAGGCGTTCCCGGTCGAtt < 2:975895/76‑1 (MQ=255) aTTACCTTTGCGCTGGATGACTTTGGTACGGGTGATGCGACCTATCGTTACTTGCAGGCGTTCCCGGTCGATTtta > 1:165515/1‑76 (MQ=255) ttACCTTTGCGCTGGATGACTTTGGTACGGGTGATGCGACCTATCGTTACTTGCAGGCGTTCCCGGTCGATTttat > 1:807962/1‑76 (MQ=255) aCCTTTGCGCTGGATGACTTTGGTACGGGTGATGCGACCTATCGTTACTTGCAGGCGTTCCCGGTCGATTttatta < 1:387485/76‑1 (MQ=255) gcTGGATGACTTTGGTACGGGTGATGCGACCTATCGTTACTTGCAGGCGTTCCCGGTCGATTTTATTAAGATCGAt < 2:194725/76‑1 (MQ=255) cTGGATGACTTTGGTACGGGTGATGCGACCTATCGTTACTTGCAGGCGTTCCCGGTCGATTTTATTAAGATCGATa < 1:1213128/76‑1 (MQ=255) tGGATGACTTTGGTACGGGTGATGCGACCTATCGTTACTTGCAGGCGTTCCCGGTCGATTTTATTAAGATCGATaa < 2:1174194/76‑1 (MQ=255) aTGACTTTGGTACGGGTGATGCGACCTATCGTTACTTGCAGGCGTTCCCGGTCGATTTTATTAAGATCGATAAGTc < 1:1223467/76‑1 (MQ=255) tGACTTTGGTACGGGTGATGCGACCTATCGTTACTTGCAGGCGTTCCCGGTCGATTTTATTAAGATCGATAAGTCa < 1:409341/76‑1 (MQ=255) cTTTGGTACGGGTTATGCGACCTATCGTTACTTGCAGGCGTTCCCGGTCGATTTTATTAAGATCGATAAGTCAttt < 1:427813/76‑1 (MQ=255) gTACGGGTGATGCGACCTATCGTTACTTGCAGGCGTTCCCGGTCGATTTTATTAAGATCGATAAGTCATTTGTGCa < 1:242962/76‑1 (MQ=255) tACGGGTGATGCGACCTATCGTTACTTGCAGGCGTTCCCGGTCGATTTTATTAAGATCGATAAGTCATTTGTGCaa < 1:298516/76‑1 (MQ=255) aCGGGTGATGCGACCTATCGTTACTTGCAGGCGTTCCCGGTCGATTTTATTAAGATCGATAAGTCATTTGTGCaaa > 2:1327915/1‑76 (MQ=255) | CAGAGCGATATTTGACAGCCTTCATCAGCACAACATTACCTTTGCGCTGGATGACTTTGGTACGGGTTATGCGACCTATCGTTACTTGCAGGCGTTCCCGGTCGATTTTATTAAGATCGATAAGTCATTTGTGCAAA > REL606/305754‑305890 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |