Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	4,154,953	T→A	intergenic (‑316/‑46)	coaA ← / → thrU	pantothenate kinase/tRNA‑Thr

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	4,154,953	0	T	A	100.0%	143.5 / NA	35	intergenic (‑316/‑46)	coaA/thrU	pantothenate kinase/tRNA‑Thr
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (23/12);  total (23/12)

TCTGCCCTCCGTTCGGCTGTTTCTTCATCGTGTCGCATAAAATGTGACCAATAAAACAAATTATGCAATTTTTTAGTTGCATGAACTCGCATGTCTCCATAGAATGCGCGCTACTTGATGCCGACTTAGCTCAGTAGGTAGAGCA  >  REL606/4154880‑4155024                                                                          |                                                                        tCTGCCCTCCGTTCGGCTGTTTCTTCATCGTGTCGCATAAAATGTGACCAATAAAACAAATTATGCAATTTTTAAg                                                                       <  1:621537/76‑1 (MQ=255)   tGCCCTCCGTTCGGCTGTTTCTTCATCGTGTCGCATAAAATGTGACCAATAAAACAAATTATGCAATTTTTAAGtt                                                                     >  2:625129/1‑76 (MQ=255)    gCCCTCCGTTCGGCTGTTTCTTCATCGTGTCGCATAAAATGTGACCAATAAAACAAATTATGCAATTTTTAAGTTg                                                                    >  2:696475/1‑76 (MQ=255)    gCCCTCCGTTCGGCTGTTTCTTCATCGTGTCGCATAAAATGTGACCAATAAAACAAATTATGCAATTTTTAAGTTg                                                                    <  2:633893/76‑1 (MQ=255)     cccTCCGTTCGGCTGTTTCTTCATCGTGTCGCATAAAATGTGACCAATAAAACAAATTATGCAATTTTTAAGTTGc                                                                   >  1:153722/1‑76 (MQ=255)        tCCGTTCGGCTGTTTCTTCATCGTGTCGCATAAAATGTGACCAATAAAACAAATTATGCAATTTTTAAGTTGCATg                                                                <  1:270094/76‑1 (MQ=255)           gTTCGGCTGTTTCTTCATCGTGTCGCATAAAATGTGACCAATAAAACAAATTATGCAATTTTTAAGTTGCATGAAc                                                             >  1:564723/1‑76 (MQ=255)           gTTCGGCTGTTTCTTCATCGTGTCGCATAAAATGTGACCAATAAAACAAATTATGCAATTTTTAAGTTGCATGAAc                                                             <  2:141205/76‑1 (MQ=255)           gTTCGGCTGTTTCTTCATCGTGTCGCATAAAATGTGACCAATAAAACAAATTATGCAATTTTTAAGTTGCATGAAc                                                             >  1:169950/1‑76 (MQ=255)             tCGGCTGTTTCTTCATCGTGTCGCATAAAATGTGACCAATAAAACAAATTATGCAATTTTTAAGTTGCATGAACTc                                                           >  2:1065746/1‑76 (MQ=255)                  tGTTTCTTCATCGTGTCGCATAAAATGTGACCAATAAAACAAATTATGCAATTTTTAAGTTGCATGAACTCGCATg                                                      >  1:568433/1‑76 (MQ=255)                   gTTTCTTCATCGTGTCGCATAAAATGTGACCAATAAAACAAATTATGCAATTTTTAAGTTGCATGAACTCGCATGt                                                     >  1:670029/1‑76 (MQ=255)                     ttcttcATCGTGTCGCATAAAATGTGACCAATAAAACAAATTATGCAATTTTTAAGTTGCATGAACTCGCATGtct                                                   <  2:865520/76‑1 (MQ=255)                             cGTGTCGCATAAAATGTGACCAATAAAACAAATTATGCAATTTTTAAGTTGCATGAACTCGCATGTCTCCATAGaa                                           >  2:739263/1‑76 (MQ=255)                              gtgtCGCATAAAATGTGACCAATAAAACAAATTATGCAATTTTTAAGTTGCATGAACTCGCATGTCTCCATAGAAt                                          <  2:626847/76‑1 (MQ=255)                               tgtCGCATAAAATGTGACCAATAAAACAAATTATGCAATTTTTAAGTTGCATGAACTCGCATGTCTCCATAGAATg                                         >  2:65273/1‑76 (MQ=255)                                gtCGCATAAAATGTGACCAATAAAACAAATTATGCAATTTTTAAGTTGCATGAACTCGCATGTCTCCATAGAATgc                                        <  1:1536868/76‑1 (MQ=255)                                  cGCATAAAATGTGACCAATAAAACAAATTATGCAATTTTTAAGTTGCATGAACTCGCATGTCTCCATAGAATgcgc                                      <  1:758172/76‑1 (MQ=255)                                    cATAAAATGTGACCAATAAAACAAATTATGCAATTTTTAAGTTGCATGAACTCGCATGTCTCCATAGAATgcgcgc                                    >  1:153702/1‑76 (MQ=255)                                      tAAAATGTGACCAATAAAACAAATTATGCAATTTTTAAGTTGCATGAACTCGCATGTCTCCATAGAATGCGCGCTa                                  <  2:88123/76‑1 (MQ=255)                                       aaaaTGTGACCAATAAAACAAATTATGCAATTTTTAAGTTGCATGAACTCGCATGTCTCCATAGAATGCGCGCTAc                                 >  1:679750/1‑76 (MQ=255)                                       aaaaTGTGACCAATAAAACAAATTATGCAATTTTTAAGTTGCATGAACTCGCATGTCTCCATAGAATGCGCGCTAc                                 >  1:978533/1‑76 (MQ=255)                                          aTGTGACCAATAAAACAAATTATGCAATTTTTAAGTTGCATGAACTCGCATGTCTCCATAGAATGCGCGCTACTTg                              >  2:656289/1‑76 (MQ=255)                                           tgtgACCAATAAAACAAATTATGCAATTTTTAAGTTGCATGAACTCGCATGTCTCCATAGAATGCGCGCTACTTGa                             >  1:1430388/1‑76 (MQ=255)                                              gACCAATAAAACAAATTATGCAATTTTTAAGTTGCATGAACTCGCATGTCTCCATAGAATGCGCGCTACTTGATGc                          >  1:1477911/1‑76 (MQ=255)                                                  aaTAAAACAAATTATGCAATTTTTAAGTTGCATGAACTCGCATGTCTCCATAGAATGCGCGCTACTTGATGCCGAc                      >  1:798374/1‑76 (MQ=255)                                                   aTAAAACAAATTATGCAATTTTTAAGTTGCATGAACTCGCATGTCTCCATAGAATGCGCGCTACTTGATGCCGACt                     >  1:1010363/1‑76 (MQ=255)                                                    tAAAACAAATTATGCAATTTTTAAGTTGCATGAACTCGCATGTCTCCATAGAATGCGCGCTACTTGATGCCGACtt                    >  2:1008850/1‑76 (MQ=255)                                                    tAAAACAAATTATGCAATTTTTAAGTTGCATGAACTCGCATGTCTCCATAGAATGCGCGCTACTTGATGCCGACtt                    >  2:219517/1‑76 (MQ=255)                                                      aaaCAAATTATGCAATTTTTAAGTTGCATGAACTCGCATGTCTCCATAGAATGCGCGCTACTTGATGCCGACTTAg                  <  2:494959/76‑1 (MQ=255)                                                      aaaCAAATTATGCAATTTTTAAGTTGCATGAACTCGCATGTCTCCATAGAATGCGCGCTACTTGATGCCGACTTAg                  <  2:955956/76‑1 (MQ=255)                                                             ttATGCAATTTTTAAGTTGCATGAACTCGCATGTCTCCATAGAATGCGCGCTACTTGATGCCGACTTAGCTCAgta           >  2:477804/1‑76 (MQ=255)                                                             ttATGCAATTTTTAAGTTGCATGAACTCGCATGTCTCCATAGAATGCGCGCTACTTGATGCCGACTTAGCTCAgta           >  1:796280/1‑76 (MQ=255)                                                                 gCAATTTTTAAGTTGCATGAACTCGCATGTCTCCATAGAATGCGCGCTACTTGATGCCGACTTAGCTCAgtaggta       >  2:298008/1‑76 (MQ=255)                                                                 gCAATTTTTAAGTTGCATGAACTCGCATGTCTCCATAGAATGCGCGCTACTTGATGCCGACTTAGCTCAgtaggta       <  2:1182758/76‑1 (MQ=255)                                                                     tttttaAGTTGCATGAACTCGCATGTCTCCATAGAATGCGCGCTACTTGATGCCGACTTAGCTCAGTAGGTAGAGc   >  2:571418/1‑76 (MQ=255)                                                                     tttttaAGTTGCATGAACTCGCATGTCTCCATAGAATGCGCGCTACTTGATGCCGACTTAGCTCAGTAGGTAGAGc   <  1:1432896/76‑1 (MQ=255)                                                                      ttttaAGTTGCATGAACTCGCATGTCTCCATAGAATGCGCGCTACTTGATGCCGACTTAGCTCAGTAGGTAGAGCa  >  1:275906/1‑76 (MQ=255)                                                                          |                                                                        TCTGCCCTCCGTTCGGCTGTTTCTTCATCGTGTCGCATAAAATGTGACCAATAAAACAAATTATGCAATTTTTTAGTTGCATGAACTCGCATGTCTCCATAGAATGCGCGCTACTTGATGCCGACTTAGCTCAGTAGGTAGAGCA  >  REL606/4154880‑4155024

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑