| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 154,497 | 0 | T | G | 23.1% | 131.4 / 10.6 | 52 | A191A (GCA→GCC) | yadL | predicted fimbrial‑like adhesin protein |
| Reads supporting (aligned to +/- strand): ref base T (4/36); new base G (12/0); total (16/36) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.82e-09 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CGTTGAGTGGGATGCATATATAGTTCCTGGATCTGTCAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCAGGTGCAGAAAATTTACCCGCAGTGACATTGCTTACGGTTTTATTTTTTTCGACGACCAGTCGAGCACTCATCGGATAA > REL606/154424‑154572 | cGTTGAGTGGGATGCATATATAGTTCCTGGATCTGTCAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCAGGTGc < 1:450515/76‑1 (MQ=255)cGTTGAGTGGGATGCATATATAGTTCCTGGATCTGTCAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCAGGTGc < 1:1059398/76‑1 (MQ=255) aGTGGGATGCATATATAGTTCCTGGATCTGTCAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCAGGTGCAGaaa < 1:1060389/76‑1 (MQ=255) gTGGGATGCATATATAGTTCCTGGATCTGTCAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCAGGTGCAGaaaa < 2:1243261/76‑1 (MQ=255) tGGGATGCATATATAGTTCCTGGATCTGTCAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCAGGGGCAGAAAAt > 1:1706943/1‑76 (MQ=255) gATGCATATATAGTTCCTGGATCTGTCAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCAGGGGGAGAAAAtttt > 2:938532/1‑75 (MQ=255) gATGCATATATAGTTCCTGGATCTGTCAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCAGGGGGAGAAAAtata > 2:62832/1‑76 (MQ=255) tGCATATATAGTTCCTGGATCTGTCAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCAGGTGCAGAAAATTTAcc < 1:1141344/76‑1 (MQ=255) atatatAGTTCCTGGATCTGTCAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCAGGGGGAGAAAAATTAccccc > 2:1267647/1‑74 (MQ=255) ataGTTCCTGGATCTGTCAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCAGGTGCAGAAAATTTACCCGCAGTg < 1:1421098/76‑1 (MQ=255) taGTTCCTGGATCTGTCAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCAGGTGCAGAAAATTTACCCGCAGTGa < 2:1737618/76‑1 (MQ=255) taGTTCCTGGATCTGTCAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCAGGTGCAGAAAATTTACCCGCAGTGa < 2:393950/76‑1 (MQ=255) taGTTCCTGGATCTGTCAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCAGGTGCAGAAAATTTACCAGCAGTGa < 2:576917/76‑1 (MQ=255) ttCCTGGATCTGTCAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCAGGGGCAGAAAATTTAACCGCAGTGACAt > 1:1879161/1‑76 (MQ=255) tCCTGGATCTGTCAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCAGGTGCAGAAAATTTACCCGCAGTGACAtt < 2:1128272/76‑1 (MQ=255) tCCTGGATCTGTCAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCAGGTGCAGAAAATTTACCCGCAGTGACAtt < 1:417751/76‑1 (MQ=255) tCCTGGATCTGTCAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCAGGTGCAGAAAATTTACCCGCAGTGACAtt < 2:640787/76‑1 (MQ=255) ccTGGATCTGTCAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCAGGTGCAGAAAATTTACCCGCAGTGACATTg < 1:381696/76‑1 (MQ=255) cTGGATCTGTCAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCAGGGGCAGAAAATTTACCCGCAGTGACATTGc > 1:1728284/1‑76 (MQ=255) tGGATCTGTCAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCAGGGGCAGAAAATTTTCCCGCAGTGACATTGCt > 2:1578575/1‑76 (MQ=255) tGGATCTGTCAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCAGGGGCAGAAAAATTAACCGCAGGGACATTGCt > 1:1471363/1‑76 (MQ=255) aTCTGTCAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCAGGTGCAGAAAATTTACCCGCAGTGACATTGCTTAc < 1:152447/76‑1 (MQ=255) aTCTGTCAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCAGGGGCAGAAAATTTACCCGCAGGGACATTGCtttc > 2:1220217/1‑74 (MQ=255) cTGTCAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCAGGTGCAGAAAATTTACCCGCAGTGACATTGCTTACgg < 1:773089/76‑1 (MQ=255) cTGTCAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCAGGGGCAGAAAAATTACCCGCCGGGACATTGCTTTCgg > 1:988142/1‑76 (MQ=255) gTCAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCAGGTGCAGAAAATTTACCCGCAGTGACATTGCTTACGGtt < 2:525943/76‑1 (MQ=255) cAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCAGGTGCAGAAAATTTACCCGCAGTGACATTGCTTTCGGtttt < 2:829472/76‑1 (MQ=255) cAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCAGGTGCAGAAAATTTACCCGCAGTGACATTGCTTACGGtttt < 1:1229122/76‑1 (MQ=255) cAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCAGGTGCAGAAAATTTACCCGCAGTGACATTGCTTACGGtttt < 2:1336529/76‑1 (MQ=255) cAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCAGGTGCAGAAAATTTACCCGCAGTGACATTGCTTACGGtttt < 2:1230110/76‑1 (MQ=255) aGTCTGTTAGTTATAGGTTACTGTAAATGTGGCAGGTGCAGAAAATTTACCCGCAGTGACATTGCTTACGGTTTTa < 2:295081/76‑1 (MQ=255) tCTGTTAGTTATAGGTTACTGTAAATGTGGCAGGGGCAGAAAAATTACCCGCCGGGGCATTTCTTACGGTtttttt > 1:1823571/1‑76 (MQ=255) tctttaTAGGTTACTGTAAATGTGGCAGGTGCAGAAAATTTACCCGCAGTGACATTGCTTACGGTTTTAttttttt < 1:928633/73‑1 (MQ=255) tagttaTAGGTTACTGTAAATGTGGCAGGTGCAGAAAATTTACCCGCAGTGACATTGCTTACGGTTTTAttttttt < 2:462162/76‑1 (MQ=255) gttaTAGGTTACTGTAAATGTGGCAGGGGCAGAAAATTTACCCGCAGTGACATTGCTTTCGGTTTTTTTTTTTTct > 2:218841/1‑75 (MQ=255) gttaTAGGTTACTGTAAATGTGGCAGGGGCAGAAAATTTACCCGCAGTGAAATTGCTTTCGGGTtttttttttttt > 1:571543/1‑74 (MQ=255) ttaTAGGTTACTGTAAATGTGGCAGGTGCAGAAAATTTACCCGCAGTGACATTGCTTACGGTTTTATTTTTTTcga < 2:1850021/76‑1 (MQ=255) ttaTAGGTTACTGTAAATGTGGCAGGGGGAGAAAATTTACCCGCAGTGACATTGCTTACGGTTTTTTTTTTTTaga > 2:928633/1‑76 (MQ=255) aGGTTACTGTAAATGTGGCAGGGGGAGAAAATTTAACCGCAGAGAAATTGCTTACGGTTTTTTTTTTTTCgaccac > 1:281243/1‑76 (MQ=255) tACTGTAAATGTGGCAGGGGCAGAAAATTTACCCGCAGTGAACTTGCTTACGGTTTTATTTTTTTTGACGACCAGt > 1:387412/1‑76 (MQ=255) cTGTAAATGTGGCAGGTGCAGAAAATTTACCCGCAGTGACATTGCTTACGGTTTTATTTTTTTCGTCGACCAGTCg < 2:962656/76‑1 (MQ=255) cTGTAAATGTGGCAGGTGCAGAAAATTTACCCGCAGTGACATTGCTTACGGTTTTATTTTTTTCGACGACCAGTCg < 2:567209/76‑1 (MQ=255) gTAAATGTGGCAGGGGCAGCAAATTTACCCGGAGTGACACTGCTTAAGGGTTTATTTTTTTTAACGGCCAGACGAg > 2:1345040/1‑76 (MQ=255) tAAATGTGGCAGGGGCAGAAAATTTACCCGCAGTGACATTGCTTACGGGTTTTTTTTTTTTGACGACCAGTCGAGc > 2:708108/1‑76 (MQ=255) aaTGTGGCAGGTGCAGAAAATTTACCCGCAGTGACATTGCTTACGGTTTTATTTTTTTCGACGACCAGTCGAGCAc < 2:139296/76‑1 (MQ=255) tgtgGCAGGTGCAGAAAATTTACCCGCAGTGACATTGCTTACGGTTTTATTTTTTTCGACGACCAGTCGAGCACTc < 2:924545/76‑1 (MQ=255) tgtgGCAGGTGCAGAAAATTTACCCGCAGTGACATTGCTTACGGTTTTATTTTTTTCGACGACCAGTCGAGCACTc < 1:785653/76‑1 (MQ=255) tgtgGCAGGTGCAGAAAATTTACCCGCAGTGACATTGCTTACGGTTTTATTTTTTTCGACGACCAGTCGAGCACTc < 2:1711567/76‑1 (MQ=255) tgGCAGGTGCAGAAAATTTACCCGCAGTGACATTGCTTACGGTTTTATTTTTTTCGACGACCAGTCGAGCACTCAt < 1:1405721/76‑1 (MQ=255) gggCAGGTGCAGAAAATTTACCCGCAGTGACATTGCTTACGGTTTTATTTTTTTCGACGACCAGTCGAGCACTCAt < 1:245705/75‑1 (MQ=255) ggCAGGTGCAGAAAATTTACCCGCAGTGACATTGCTTACGGTTTTATTTTTTTCGACGACCAGTCGAGCACTCATc > 2:1849291/1‑76 (MQ=255) cAGGTGCAGAAAATTTACCCGCAGTGACATTGCTTACGGTTTTATTTTTTTCGACGACCAGTCGAGCACTCATCgg > 1:328935/1‑76 (MQ=255) cAGGTGCAGAAAATTTACCCGCAGTGACATTGCTTACGGTTTTATTTTTTTCGACGACCAGTCGAGCACTCATCgg > 2:1083343/1‑76 (MQ=255) ggTGCAGAAAATTTACCCGCAGTGACATTGCTTACGGTTTTATTTTTTTCGACGACCAGTCGAGCACTCATCGGat < 1:1621729/76‑1 (MQ=255) ggTGCAGAAAATTTACCCGCAGTGACATTGCTTACGGTTTTATTTTTTTCGACGACCAGTCGAGCACTCATCGGat < 1:1707034/76‑1 (MQ=255) ggTGCAGAAAATTTACCCGCAGTGACATTGCTTACGGTTTTATTTTTTTCGACGACCAGTCGAGCACTCATCGGat < 1:132200/76‑1 (MQ=255) gTGCAGAAAATTTACCCGCAGTGACATTGCTTACGGTTTTATTTTTTTCGACGACCAGTCGAGCACTCATCGGata < 1:1404004/76‑1 (MQ=255) gTGCAGAAAATTTACCCGCAGTGACATTGCTTACGGTTTTATTTTTTTCGACGACCAGTCGAGCACTCATCGGata > 1:324353/1‑76 (MQ=255) tGCAGAAAATTTACCCGCAGTGACATTGCTTACGGTTTTATTTTTTTCGACGACCAGTCGAGCACTCATCGGataa > 1:825032/1‑76 (MQ=255) | CGTTGAGTGGGATGCATATATAGTTCCTGGATCTGTCAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCAGGTGCAGAAAATTTACCCGCAGTGACATTGCTTACGGTTTTATTTTTTTCGACGACCAGTCGAGCACTCATCGGATAA > REL606/154424‑154572 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |