| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 2,417,495 | 0 | T | A | 20.3% | 176.5 / 10.8 | 59 | L24F (TTA→TTT) | emrK | EmrKY‑TolC multidrug resistance efflux pump, membrane fusion protein component |
| Reads supporting (aligned to +/- strand): ref base T (39/8); new base A (0/12); total (39/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.13e-07 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTAT > REL606/2417421‑2417569 | tAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCaataaata > 1:1516092/1‑76 (MQ=255)tAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCaataaata > 1:183857/1‑76 (MQ=255)tAGGCGCCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCaataaata > 1:183853/1‑76 (MQ=255) aGGCGTCATCTGTCCTAATCATGTCTTCTAATTCCATTGACCAATAGGCACAGGCACCTGAAAACGCAataaataa > 2:868220/1‑76 (MQ=255) aGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCAataaataa > 2:868239/1‑76 (MQ=255) aGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCAataaataa > 2:1229417/1‑76 (MQ=255) cGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAAc > 1:109483/1‑76 (MQ=255) cGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAAc > 1:1616074/1‑76 (MQ=255) gTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACt < 2:517300/76‑1 (MQ=255) tCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTa < 1:979376/76‑1 (MQ=255) tCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTa < 1:162798/76‑1 (MQ=255) tcccTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCA‑CTGAAAACGGCATAAATAAAaccacc > 2:890987/4‑76 (MQ=255) aTCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTAcc > 1:1474391/1‑76 (MQ=255) tCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCg > 1:1128062/1‑76 (MQ=255) tCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCg > 1:1128041/1‑76 (MQ=255) tCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCg < 1:59053/76‑1 (MQ=255) cTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGc < 1:1335837/76‑1 (MQ=255) tGTACTAATCATGTCTTCTAATTCCCTTGACCACTAGGCATAGGCACCTCACAACGCAATAAATACAACTAACGcc > 1:47637/1‑76 (MQ=255) tGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACt < 1:1087449/70‑1 (MQ=255) tGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACt > 2:1087449/1‑70 (MQ=255) tGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGcc > 1:47601/1‑76 (MQ=255) tGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCACAGGCACCTGAAAACGCAATAAATAAAACTACCGcc > 1:47606/1‑76 (MQ=255) gTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGCCa > 2:1195618/1‑76 (MQ=255) tAATCATGTTTTCAAATTCCATTGACAAATAGGCATAGGCCCCTAAAAACGAAAAAAAAAAAACTACCGCCAATaa < 2:1831450/76‑1 (MQ=255) aaTCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGCCAATaaa > 2:297130/1‑76 (MQ=255) aTCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGCCAATAAAg > 1:201347/1‑76 (MQ=255) aTCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGCCAATAAAg > 1:201356/1‑76 (MQ=255) aTCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGCCAATAAAg > 1:361977/1‑76 (MQ=255) aTCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGCCAATAAAg > 1:480835/1‑76 (MQ=255) tCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGa > 1:782469/1‑76 (MQ=255) gTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAg > 2:439311/1‑76 (MQ=255) gTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAg > 2:439321/1‑76 (MQ=255) nttctAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAGTa > 1:545308/2‑76 (MQ=255) cttctAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAGTa > 2:1773757/1‑76 (MQ=255) cttctAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAGTa > 1:1617004/1‑76 (MQ=255) cttctAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAGTa > 1:1616990/1‑76 (MQ=255) ttctAATTCCATTGACAAATAGGCATAGGCACCAAAAAACGAAAAAAAAAAAACTACCGCCAATAAAGAAAAGTAt < 1:1286633/76‑1 (MQ=255) ttctAATCCCATTGACAAATAGGCATAGGCACCTAAAAACGAAAAAAAAAAAACTACCGCCAATAAAGAAAAGTAt < 1:1090374/76‑1 (MQ=255) tctAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAGTAtt > 2:360172/1‑76 (MQ=255) tctAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGAAAAAAAAAAAACTACCGCCAATAAAGAAAAGTAtt < 2:1649719/76‑1 (MQ=255) tctAATTCCATTGACCAATAGGCATAGGCACCTAAAAACGAAAAAAAAAAAACTACCGCCAATAAAGAAAAGTAtt < 2:1210118/76‑1 (MQ=255) tAATTCCATTGACAAATAGGCATAGGCACCTAAAACCGAAAAAAAAAAAACTACCGCCAATAAAGAAAAGTAtttt < 1:1472548/76‑1 (MQ=255) aaTTCCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAGTATTttc > 1:936406/1‑76 (MQ=255) ttCCATTGACCAATAGGCATAGGCACCTGAAAACGAAAAAAATAAAACTACCGCCAATAAAGAAAAGTATTttctt < 2:1459760/76‑1 (MQ=255) tCCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAGTATTttcttc > 2:543415/1‑76 (MQ=255) cATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTg > 2:1784631/1‑76 (MQ=255) cATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTg > 1:1806256/1‑76 (MQ=255) gACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAg > 2:1538308/1‑76 (MQ=255) gACCAAAAGGAATAGGCACCTAAAAACGAAAAAAAAAAACCTCCCGCCAATAAAGAAAAGTATTTTCTTCTGTTAg < 2:461270/76‑1 (MQ=255) gACAAATAGGCAGAGGCCCCAAAAAACAAAAAAAAAAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAg < 1:1292709/76‑1 (MQ=255) ccAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGaa > 1:1256875/1‑76 (MQ=255) ccAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGaa > 1:121445/1‑76 (MQ=255) ccAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAGTATTCTCTTCTGTTAGaa > 1:798348/1‑76 (MQ=255) caaaTAGGCATAGGCACCTGAAAACGAAAAAAAAAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGaa < 2:571991/74‑1 (MQ=255) cAATAGGCATAGGCACCTGAAAACGCAATAAATAAACCTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAAt < 2:1514509/76‑1 (MQ=255) cAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAAt > 1:834740/1‑76 (MQ=255) ataggcataggcACCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGt > 2:250358/1‑76 (MQ=255) ataggcatagccACCTAAAAACGAAAAAAAAAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGt < 2:543668/76‑1 (MQ=255) taggcataggcaCCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGtt > 2:705779/1‑76 (MQ=255) aggcataggcaCCTAAAAACGAAAAAAAAAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGttt < 1:315505/76‑1 (MQ=255) gcataggcaCCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGttttt > 1:1313611/1‑76 (MQ=255) cataggcaCCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGtttttt > 2:1719045/1‑76 (MQ=255) cataggcaCCTGAAAACGCAATAAATAAAACTACCGCAAATAAAGAAAAGTATTTTCTTCTGTTAGAATGtttttt > 2:1719015/1‑76 (MQ=255) ataggcaCCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTa > 1:545312/1‑76 (MQ=255) taggcaCGGAAAAACGAAAAAAAAAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTAt < 1:630803/76‑1 (MQ=255) taggcaCCTAAAAACGAAAAAAAAAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTAt < 1:1730780/76‑1 (MQ=255) aggcaCCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTAtt > 1:897019/1‑76 (MQ=255) aggcaCCTAAAAACGAAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTAtt < 2:1880228/76‑1 (MQ=255) cccTGAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGaa < 2:1762852/75‑1 (MQ=255) ccTGAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAAt > 1:787148/1‑76 (MQ=255) ctaaaaaCGAAAAAAAAAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAAtt < 1:1691325/73‑1 (MQ=255) cTGAAAACGCAATAAAAAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAAtt < 1:1691309/76‑1 (MQ=255) tGAAAACGAAATAAAAAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTa < 2:109179/76‑1 (MQ=255) gaaaaaCGAAAAAAATAAAACAACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTa < 2:860385/74‑1 (MQ=255) aaaaaCGAAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTaa < 2:993756/75‑1 (MQ=255) aaaaaCAAAAAAAAAAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTaa < 2:794333/75‑1 (MQ=255) aCGCAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTg < 1:1891863/76‑1 (MQ=255) aCGAAAAAAAAAAACCTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTg < 1:1163528/76‑1 (MQ=255) aaaaaaaaaaaCTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTcc < 2:960726/73‑1 (MQ=255) ataaaaaaATCAGCCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCa < 2:451665/76‑1 (MQ=255) aaaaaaaaaaCTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCa < 1:297130/74‑1 (MQ=255) aaaaaaaaaaCTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCa < 1:572036/74‑1 (MQ=255) taaataaaACTACCGCCAATAAAGAAAAGTATTTTATTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCAc > 2:133253/1‑76 (MQ=255) aaaaaaaaCTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACt < 1:635951/76‑1 (MQ=255) aaaaaaaaCTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACt < 1:505316/76‑1 (MQ=255) aataaaACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACta > 1:878961/1‑76 (MQ=255) aataaaACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACta > 1:26828/1‑76 (MQ=255) ataaaACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACtat > 1:1713799/1‑76 (MQ=255) | TAGGCGTCATCTGTACTAATCATGTCTTCTAATTCCATTGACCAATAGGCATAGGCACCTGAAAACGCAATAAATAAAACTACCGCCAATAAAGAAAAGTATTTTCTTCTGTTAGAATGTTTTTTATTTGAATTAATCTGTTCCACTAT > REL606/2417421‑2417569 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |