Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	3,252,016	C→T	G249S (GGT→AGT)	nusA ←	transcription elongation factor NusA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	3,252,016	0	C	T	100.0%	164.0 / NA	42	G249S (GGT→AGT)	nusA	transcription elongation factor NusA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (18/24);  total (18/24)

TATCGATACGCTCGCCACCCAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAGCACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGCGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTG  >  REL606/3251944‑3252087                                                                         |                                                                        tatCGATACGCTCGCCACCCAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAGCACTTAc                                                                      <  1:1150173/76‑1 (MQ=255) tatCGATACGCTCGCCACCCAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAGCACTTAc                                                                      >  1:1280889/1‑76 (MQ=255) tatCGATACGCTCGCCACCCAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAGCACTTAc                                                                      >  1:1767097/1‑76 (MQ=255)   tCGATACGCTCGCCACCCAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAGCACTTACCg                                                                    <  1:1526992/76‑1 (MQ=255)    cGATACGCTCGCCACCCAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAGCACTTACCgg                                                                   <  1:1040703/76‑1 (MQ=255)    cGATACGCTCGCCACCCAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAGCACTTACCgg                                                                   >  1:589202/1‑76 (MQ=255)      aTACGCTCGCCACCCAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAGCACTTACCGGAt                                                                 <  2:1796385/76‑1 (MQ=255)       tACGCTCGCCACCCAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAGCACTTACCGGATc                                                                >  1:618733/1‑76 (MQ=255)           cTCGCCCCCCAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAGCACTTACCGGATCGATa                                                            <  1:1281170/76‑1 (MQ=255)           cTCGCCACCCAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAGCACTTACCGGATCGATa                                                            <  1:23664/76‑1 (MQ=255)            tCGCCACCCAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAGCACTTACCGGATCGATAc                                                           <  1:295848/76‑1 (MQ=255)            tCGCCACCCAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAGCACTTACCGGATCGATAc                                                           >  2:39545/1‑76 (MQ=255)            tCGCCACCCAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAGCACTTACCGGATCGATAc                                                           >  1:469222/1‑76 (MQ=255)            tCGCCACCCAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAGCACTTACCGGATCGATAc                                                           <  1:1423490/76‑1 (MQ=255)                cACCCAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAGCACTTACCGGATCGATACGttt                                                       <  1:328371/76‑1 (MQ=255)                cACCCAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAGCACTTACCGGATCGATACGttt                                                       <  2:1785676/76‑1 (MQ=255)                cACCCAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAGCACTTACCGGATCGATACGttt                                                       <  1:805678/76‑1 (MQ=255)                cACCCAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAGCACTTACCGGATCGATACGttt                                                       >  2:1528949/1‑76 (MQ=255)                 aCCCAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAGCACTTACCGGATCGATACGTTTa                                                      <  1:646461/76‑1 (MQ=255)                  cccAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAGCACTTACCGGATCGATACGTTTAt                                                     <  1:428934/76‑1 (MQ=255)                    cAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAGCACTTACCGGATCGATACGTTTATCg                                                   <  2:864752/76‑1 (MQ=255)                    cAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAGCACTTACCGGATCGATACGTTTATCg                                                   >  1:1228011/1‑76 (MQ=255)                     aGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAGCACTTACCGGATCGATACGTTTATCGt                                                  >  2:1868819/1‑76 (MQ=255)                            tAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAGCACTTACCGGATCGATACGTTTATCGTTGGtttt                                           <  2:1835131/76‑1 (MQ=255)                            tAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAGCACTTACCGGATCGATACGTTTATCGTTGGtttt                                           <  2:351875/76‑1 (MQ=255)                            tAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAGCACTTACCGGATCGATACGTTTATCGTTGGtttt                                           >  2:674244/1‑76 (MQ=255)                             aGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAGCACTTACCGGATCGATACGTTTATCGTTGGTTTTc                                          <  2:1768789/76‑1 (MQ=255)                                      cTGAACACGCGCGCCACGCATACCTACGCAAGCACTTACCGGATCGATACGTTTATCGTTGGTTTTCACCGCGAtt                                 <  1:1138029/76‑1 (MQ=255)                                        gAACACGCGCGCCACGCATACCTACGCAAGCACTTACCGGATCGATACGTTTATCGTTGGTTTTCACCGCGAtttt                               <  2:1127730/76‑1 (MQ=255)                                                 cgcCACGCATACCTACGCAAGCACTTACCGGATCGATACGTTTATCGTTGGTTTTCACCGCGATTTTCGCACgaga                      <  2:122749/76‑1 (MQ=255)                                                  gcCACGCATACCTACGCAAGCACTTACCGGATCGATACGTTTATCGTTGGTTTTCACCGCGATTTTCGCACGAGaa                     <  1:538044/76‑1 (MQ=255)                                                   ccACGCATACCTACGCAAGCACTTACCGGATCGATACGTTTATCGTTGGTTTTCACCGCGATTTTCGCACGAGAAc                    >  2:138832/1‑76 (MQ=255)                                                    cACGCATACCTACGCAAGCACTTACCGGATCGATACGTTTATCGTTGGTTTTCACCGCGATTTTCGCACGAGAAcc                   <  2:35071/76‑1 (MQ=255)                                                     aCGCATACCTACGCAAGCACTTACCGGATCGATACGTTTATCGTTGGTTTTCACCGCGATTTTCGCACGAGAAccc                  <  2:876386/76‑1 (MQ=255)                                                      cGCATACCTACGCAAGCACTTACCGGATCGATACGTTTATCGTTGGTTTTCACCGCGATTTTCGCACGAGAACCCg                 >  1:921747/1‑76 (MQ=255)                                                        cATACCTACGCAAGCACTTACCGGATCGATACGTTTATCGTTGGTTTTCACCGCGATTTTCGCACGAGAACCCGGa               >  1:1406635/1‑76 (MQ=255)                                                         aTACCTACGCAAGCACTTACCGGATCGATACGTTTATCGTTGGTTTTCACCGCGATTTTCGCACGAGAACCCGGAt              >  2:385289/1‑76 (MQ=255)                                                          tACCTACGCAAGCACTTACCGGATCGATACGTTTATCGTTGGTTTTCACCGCGATTTTCGCACGAGAACCCGGATc             <  2:1557665/76‑1 (MQ=255)                                                               aCGCAAGCACTTACCGGATCGATACGTTTATCGTTGGTTTTCACCGCGATTTTCGCACGAGAACCCGGATCGCGAg        >  1:1323360/1‑76 (MQ=255)                                                                   aaGCACTTACCGGATCGATACGTTTATCGTTGGTTTTCACCGCGATTTTCGCACGAGAACCCGGATCGCGAGCCgc    >  2:1724667/1‑76 (MQ=255)                                                                   aaGCACTTACCGGATCGATACGTTTATCGTTGGTTTTCACCGCGATTTTCGCACGAGAACCCGGATCGCGAGCCgc    >  1:625042/1‑76 (MQ=255)                                                                     gCACTTACCGGATCGATACGTTTATCGTTGGTTTTCACCGCGATTTTCGCACGAGAACCCGGATCGCGAGCCgctg  >  1:676021/1‑76 (MQ=255)                                                                         |                                                                        TATCGATACGCTCGCCACCCAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAGCACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGCGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTG  >  REL606/3251944‑3252087

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑