| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 2,781,691 | A→T | I179F (ATC→TTC) | ygcN → | predicted oxidoreductase with FAD/NAD(P)‑binding domain |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 2,781,691 | 0 | A | T | 94.9% | 214.8 / 2.1 | 60 | I179F (ATC→TTC) | ygcN | predicted oxidoreductase with FAD/NAD(P)‑binding domain |
| Reads supporting (aligned to +/- strand): ref base A (0/3); new base T (24/32); total (25/35) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.64e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.16e-01 | |||||||||||
AGGTGCCAACAGCGTCCTGGCTGAACGTCACGGGTTAGTGACTCGTCCTGCTGGCGAAGCGATGGCGTTGGGGATCAAAGAAGTGCTGTCGCTGGAAACATCCGCTATTGAAGAACGTTTTCATCTGGAGAATAACGAAGGCGCAG > REL606/2781618‑2781763 | aGGTGCCAACAGCGTCCTGGCTGAACGTCACGGGTTAGTGACTCGTCCTGCTGGCGAAGCGATGGCGTTGGGGTTc > 2:1652452/1‑76 (MQ=255)aGGTGCCAACAGCGTCCTGGCTGAACGTCACGGGTTAGTGACTCGTCCTGCTGGCGAAGCGATGGCGTTGGGGTTc < 2:924420/76‑1 (MQ=255) ggTGCCAGCAGCGTCCTGGCTGAACGTCACGGGTTAGTGACTCGTCCTGCTGGCGAAGCGATGGCGTTGGGGTTCa < 2:163009/76‑1 (MQ=255) ggTGCCAACAGCGTCCTGGCTGAACGTCACGGGTTAGTGACTCGTCCTGCTGGCGAAGCGATGGCGTTGGGGTTCa < 1:913599/76‑1 (MQ=255) gTGCCAACAGCGTCCTGGCTGAACGTCACGGGTTAGTGACTCGTCCTGCTGGCGAAGCGATGGCGTTGGGGATCaa < 1:209971/76‑1 (MQ=255) gCCAACAGCGTCCTGGCTGAACGTCACGGGTTAGTGACTCGTCCTGCTGGCGAAGCGATGGCGTTGGGGGTCAaag > 1:1003959/1‑76 (MQ=255) aaCAGCGTCCTGGCTGAACGTCACGGGTTAGTGACTCGTCCTGCTGGCGAAGCGATGGCGTTGGGGTTCAaagaag < 2:1620248/76‑1 (MQ=255) aaCAGCGTCCTGGCTGAACGTCACGGGTTAGTGACTCGTCCTGCTGGCGAAGCGATGGCGGTGGGGGTCAaagaag > 2:455313/1‑76 (MQ=255) cAGCGTCCTGGCTGAACGTCACGGGTTAGTGACTCGTCCTGCTGGCGAAGCGATGGCGTTGGGGTTCAAAGAAGTg > 1:1388436/1‑76 (MQ=255) cAGCGTCCTGGCTGAACGTCACGGGTTAGTGACTCGTCCTGCTGGCGAAGCGATGGCGTTGGGGTTCAAAGAAGTg > 2:1480305/1‑76 (MQ=255) aGCGTCCTGGCTGAACGTCACGGGTTAGTGACTCGTCCTGCTGGCGAAGCGATGGCGTTGGGGTTCAAAGAAGTGc < 1:1127846/76‑1 (MQ=255) cGTCTTGGCTTACCGTCACGGGTCAGTGACTCGTCCTGCTGGCGAAGCGATGGCGTTGGGGTTCAAAGAAGTGCTg < 1:750466/76‑1 (MQ=255) cGTCCTGGCTGAACGTCACGGGTTAGTGACTCGTCCTGCTGGCGAAGCGATGGCGTTGGGGTTCAAAGAAGTGCTg > 1:475847/1‑76 (MQ=255) cGTCCTGGCTGAACGTCACGGGTTAGTGACTCGTCCTGCTGGCGAAGCGATGGCGTTGGGGTTCAAAGAAGTGCTg < 2:112217/76‑1 (MQ=255) tCCTGGCTGAACGTCACGGGTTAGTGACTCGTCCTGCTGGCGAAGCGATGGCGTTGGGGTTCAAAGAAGTGCTGTc < 2:239495/76‑1 (MQ=255) cTGGCTGAACGTCACGGGTTAGTGACTCGTCCTGCTGGCGAAGGGATGGCGTTGGGGTTCAAAGAAGTGCTGTCGc > 2:642516/1‑76 (MQ=255) cTGGCTGAACGTCACGGGTTAGTGACTCGTCCTGCTGGCGAAGCGATGGCGTTGGGGTTCAAAGAAGTGCTGtaag < 2:1290876/76‑4 (MQ=255) cTGGCTGAACGTCACGGGTTAGTGACTCGTCCTGCTGGCGAAGCGATGGCGTTGGGGTTCAAAGAAGTGCTGTCGc > 1:1782873/1‑76 (MQ=255) tGGCTGAACGTCACGGGTTAGTGACTCGTCCTGCTGGCGAAGCGATGGCGTTGGGGTTCAAAGAAGTGCTGTCGCt < 1:1427360/76‑1 (MQ=255) ggCTGAACGTCACGGGTTAGTGACTCGTCCTGCTGGCGAAGCGATGGCGTTGGGGTTCAAAGAAGTGCTGTCGCTg > 1:792393/1‑76 (MQ=255) gCTGAACGTCACGGGTTAGTGACTCGTCCTGCTGGCGAAGCGATGGCGTTGGGGTTCAAAGAAGTGCTGTCGCTgg > 1:1225636/1‑76 (MQ=255) cTGAACGTCACGGGTTAGTGACTCGTCCTGCTGGCGAAGCGATGGCGTTGGGGTTCAAAGAAGTGCTGTCGCTGGa < 1:1095780/76‑1 (MQ=255) tGAACGTCACGGGTTAGTGACTCGTCCTGCTGGCGAAGCGATGGCGTTGGGGTTCAAAGAAGTGCTGTCGCTGGaa > 1:404997/1‑76 (MQ=255) gAACGTCACGGGTTAGTGACTCGTCCTGCTGGCGAAGCGATGGCGTTGGGGTTCAAAGAAGTGCTGTCGCTGGaaa > 2:1262570/1‑76 (MQ=255) gAACGTCACGGGTTAGTGACTCGTCCTGCTGGCGAAGCGATGGCGTTGGGGTTCAAAGAAGTGCTGTCGCTGGaaa < 1:324119/76‑1 (MQ=255) aaCGTCACGGGTTAGTGACTCGTCCTGCTGGCGAAGCGATGGCGTTGGGGTTCAAAGAAGTGCTGTCGCTGGAAAc < 1:800220/76‑1 (MQ=255) tCACGGGTTAGTGACTCGTCCTGCTGGCGAAGCGATGGCGTTGGGGTTCAAAGAAGTGCTGTCGCTGGGAACATcc > 2:874957/1‑76 (MQ=255) cACGGGTTAGTGACTCGTCCTGCTGGCGAAGCGATGGCGTTGGGGTTCAAAGAAGTGCTGTCGCTGGAAACATCCg > 1:355882/1‑76 (MQ=255) cACGGGTTAGTGACTCGTCCTGCTGGCGAAGCGATGGCGTTGGGGTTCAAAGAAGTGCTGTCGCTGGAAACATCCg > 2:980630/1‑76 (MQ=255) cGGGTTAGTGACTCGTCCTGCTGGCGAAGCGATGGCGTTGGGGTTCAAAGAAGTGCTGTCGCTGGAAACATCCGCt < 1:1862086/76‑1 (MQ=255) cGGGTTAGTGACTCGTCCTGCTGGCGAAGCGATGGCGTTGGGGTTCAAAGAAGTGCTGTCGCTGGAAACATCCGCt > 2:1836547/1‑76 (MQ=255) gTTAGTGACTCGTCCTGCTGGCGAAGCGATGGCGTTGGGGTTCAAAGAAGTGCTGTCGCTGGAAACATCCGCTAtt < 1:1366747/76‑1 (MQ=255) tAGTGACTCGTCCTGCTGGCGAAGCGATGGCGTTGGGGTTCAAAGAAGTGCTGTCGCTGGAAACATCCGCTATTga < 2:1603477/76‑1 (MQ=255) tAGTGACTCGTCCTGCTGGCGAAGCGATGGCGTTGGGGTTCAAAGAAGTGCTGTCGCTGGAAACATCCGCTATTga < 1:1480305/76‑1 (MQ=255) gTGACTCGTCCTGCTGGCGAAGCGATGGCGTTGGGGTTCAAAGAAGTGCTGTCGCTGGAAACATCCGCTATTgaag < 2:877571/76‑1 (MQ=255) gACTCGTCCTGCTGGCGAAGCGATGGCGTTGGGGTTCAAAGAAGTGCTGTCGCTGGAAACATCCGCTATTgaagaa < 1:165918/76‑1 (MQ=255) gACTCGTCCTGCTGGCGAAGCGATGGCGTTGGGGTTCAAAGAAGTGCTGTCGCTGGAAACATCCGCTATTgaagaa > 1:1176224/1‑76 (MQ=255) gACTCGTCCTGCTGGCGAAGCGATGGCGTTGGGGTTCAAAGAAGTGCTGTCGCTGGAAACATCCGCTATTgaagaa > 2:5838/1‑76 (MQ=255) cTCGTCCTGCTGGCGAAGCGATGGCGTTGGGGTTCAAAGAAGTGCTGTCGCTGGAAACATCCGCTATTGAAGAACg > 2:1337833/1‑76 (MQ=255) cTCGTCCTGCTGGCGAAGCGATGGCGTTGGGGTTCAAAGAAGTGCTGTCGCTGGAAACATCCGCTATTGAAGAACg > 1:1031149/1‑76 (MQ=255) cTCGTCCTGCTGGCGAAGCGATGGCGTTGGGGATCAAAGAAGTGCTGTCGCTGGAAACATCCGCTATTGAAGAACg < 2:1614793/76‑1 (MQ=255) gTCCTGCTGGCGAAGCGATGGCGTTGGGGTTCAAAGAAGTGCTGTCGCTGGAAACATCCGCTATTGAAGAACGttt < 1:115368/76‑1 (MQ=255) ccTGCTGGCGAAGCGATGGCGTTGGGGTTCAAAGAAGTGCTGTCGCTGGAAACATCCGCTATTGAAGAACGTTTTc < 1:211820/76‑1 (MQ=255) ctgctgGCGAAGCGATGGCGTTGGGGTTCAAAGAAGTGCTGTCGCTGGAAACATCCGCTATTGAAGAACGTTTTCa > 2:562228/1‑76 (MQ=255) tgctgGCGAAGCGATGGCGTTGGGGTTCAAAGAAGTGCTGTCGCTGGAAACATCCGCTATTGAAGAACGTTTTCAt < 1:507282/76‑1 (MQ=255) gctgGCGAAGCGATGGCGTTGGGGTTCAAAGAAGTGCTGTCGCTGGAAACATCCGCTATTGAAGAACGTTTTCATc > 2:159377/1‑76 (MQ=255) ctgGCGAAGCGATGGCGTTGGGGTTCAAAGAAGTGCTGTCGCTGGAAACATCCGCTATTGAAGAACGTTTTCATCt < 2:1081275/76‑1 (MQ=255) ctgGCGAAGCGATGGCGTTGGGGTTCAAAGAAGTGCTGTCGCTGGAAACATCCGCTATTGAAGAACGTTTTCATCt < 1:1784602/76‑1 (MQ=255) tgGCGAAGCGATGGCGTTGGGGTTCAAAGAAGTGCTGTCGCTGGAAACATCCGCTATTGAAGAACGTTTTCATCTg > 2:87748/1‑76 (MQ=255) ggCGAAGCGATGGCGTTGGGGTTCAAAGAAGTGCTGTCGCTGGAAACATCCGCTATTGAAGAACGTTTTCATCTgg > 1:1384611/1‑76 (MQ=255) cGAAGCGATGGCGTTGGGGTTCAAAGAAGTGCTGTCGCTGGAAACATCCGCTATTGAAGAACGTTTTCATCTGgag < 1:109430/76‑1 (MQ=255) cGAAGCGATGGCGTTGGGGTTCAAAGAAGTGCTGTCGCTGGAAACATCCGCTATTGAAGAACGTTTTCATCTGgag < 1:1295624/76‑1 (MQ=255) cGATGGCGTTGGGGTTCAAAGAAGTGCTGTCGCTGGAAACATCCGCTATTGAAGAACGTTTTCATCTGGAGAATaa < 1:1116405/76‑1 (MQ=255) aTGGCGTTGGGGTTCAAAGAAGTGCTGTCGCTGGAAACATCCGCTATTGAAGAACGTTTTCATCTGGAGAATAACg > 2:1049281/1‑76 (MQ=255) tGGCGTTGGGGTTCAAAGAAGTGCTGTCGCTGGAAACATCCGCTATTGAAGAACGTTTTCATCTGGAGAATAACGa > 1:1552073/1‑76 (MQ=255) tGGCGTTGGGGTTCAAAGAAGTGCTGTCGCTGGAAACATCCGCTATTGAAGAACGTTTTCATCTGGAGAATAACGa < 2:264645/76‑1 (MQ=255) gCGTTGGGGTTCAAAGAAGTGCTGTCGCTGGAAACATCCGCTATTGAAGAACGTTTTCATCTGGAGAATAACGAAg < 2:404997/76‑1 (MQ=255) gTTGGGGATCAAAGAAGTGCTGTCGCTGGAAACATCCGCTATTGAAGAACGTTTTCATCTGGAGAATAACGAAGgc < 2:1677833/76‑1 (MQ=255) tGGGGTTCAAAGAAGTGCTGTCGCTGGAAACATCCGCTATTGAAGAACGTTTTCATCTGGAGAATAACGAAGgcgc < 1:1735830/76‑1 (MQ=255) ggggTTCAAAGAAGTGCTGTCGCTGGAAACATCCGCTATTGAAGAACGTTTTCATCTGGAGAATAACGAAGGCGCa < 1:1457294/76‑1 (MQ=255) cgggTTCAAAGAAGTGCTGTCGCTGGAAACATCCGCTATTGAAGAACGTTTTCATCTGGAGAATAACGAAGGCGCa > 1:792121/2‑76 (MQ=255) gggTTCAAAGAAGTGCTGTCGCTGGAAACATCCGCTATTGAAGAACGTTTTCATCTGGAGAATAACGAAGGCGCAg < 1:1596110/76‑1 (MQ=255) | AGGTGCCAACAGCGTCCTGGCTGAACGTCACGGGTTAGTGACTCGTCCTGCTGGCGAAGCGATGGCGTTGGGGATCAAAGAAGTGCTGTCGCTGGAAACATCCGCTATTGAAGAACGTTTTCATCTGGAGAATAACGAAGGCGCAG > REL606/2781618‑2781763 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |