Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC	REL606	474,393	Δ9 bp	coding (905‑913/1677 nt)	ybaL ←	predicted transporter with NAD(P)‑binding Rossmann‑fold domain

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	REL606	= 474392	1 (0.020)	50 (0.830)	39/150	0.6	98.0%	coding (914/1677 nt)	ybaL	predicted transporter with NAD(P)‑binding Rossmann‑fold domain
	?	REL606	474402 =	1 (0.020)					coding (904/1677 nt)	ybaL	predicted transporter with NAD(P)‑binding Rossmann‑fold domain

AGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCGTCGCCAGCA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  REL606/474318‑474392 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GTTGCTGAATCAGAATTAACGGATCAAACAACATCCCGACGGAGACAAAAAACAGCACCGCAAACGCGTCGCGC  >  REL606/474402‑474475                                                                                                                                                       AGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCGTCGCCAGCAG                                                                           <  2:1868634/76‑1  GTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCGTCGCCAGCAGT                                                                          <  2:1063421/76‑1    GACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCGTCGCCAGCAGTTG                                                                        >  2:300902/1‑76        AAACAGTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCGTCGCCAGCAGTTGCTGA                                                                    >  2:1804218/1‑76          ACAGTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCGTCGCCAGCAGTTGCTGAAT                                                                  <  2:123480/76‑1            AGTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAAAAATCGCCAGCGTCGCCAGCAGTTGCTGAATCA                                                                <  1:887209/76‑1             GTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCGTCGCCAGCAGTTGCTGAATCAG                                                               <  1:1067327/76‑1             GTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCGTCGCCAGCAGTTGCTGAATCAG                                                               <  2:237413/76‑1             GTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCGTCGCCAGCAGTTGCTGAATCAG                                                               <  1:1807902/76‑1             GTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCGTCGCCAGCAGTTGCTGAATCAG                                                               <  1:1304339/76‑1              TCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCGTCGCCAGCAGTTGCTGAATCAGA                                                              >  1:789141/1‑76               CGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCGTCGCCAGCAGTTGCTGAATCAGAA                                                             >  1:1449091/1‑76                GCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCGTCGCCAGCAGTTGCTGAATCAGAAT                                                            >  1:407891/1‑76                 CACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCGTCGCCAGCAGTTGCTGAATCAGAATT                                                           <  2:467907/76‑1                    CAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCGTCGCCAGCAGTTGCTGAATCAGAATTAAC                                                        >  1:448410/1‑76                    CAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCGTCGCCAGCAGTTGCTGAATCAGAATTAAC                                                        <  1:1244318/76‑1                     AGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCGTCGCCAGCAGTTGCTGAATCAGAATTAACG                                                       >  1:744832/1‑76                             TGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCGTCGCCAGCAGTTGCTGAATCAGAATTAACGGATCAAAC                                               <  2:62607/76‑1                               CGGCTAACGACTTACCAAACAGAATAATCGCCAGCGTCGCCAGCAGTTGCTGAATCAGAATTAACGGATCAAACAA                                             <  2:681544/76‑1                               CGGCTAACGACTTACCAAACAGAATAATCGCCAGCGTCGCCAGCAGTTGCTGAATCAGAATTAACGGATCAAACAA                                             <  1:232542/76‑1                               CGGCTAACGACTTACCAAACAGAATAATCGCCAGCGTCGCCAGCAGTTGCTGAATCAGAATTAACGGATCAAACAA                                             <  2:130674/76‑1                                GGCTAACGACTTACCAAAAAAAATAATCGCCAGCGTCGCCAGCAGTTGCTGAATCAGAATTAACGGATCAAACAAC                                            <  1:1262748/76‑1                                 GCTAACGACTTACCAAACAGAATAATCGCCAGCGTCGCCAGCAGTTGCTGAATCAGAATTAACGGATCAAACAACA                                           >  1:1229519/1‑76                                  CTAACGACTTACCAAACAGAATAATCGCCAGCGTCGCCAGCAGTTGCTGAATCAGAATTAACGGATCAAACAACAT                                          <  1:590318/76‑1                                            ACCAAACAGAATAATCGCCAGCGTCGCCAGCAGTTGCTGAATCAGAATTAACGGATCAAACAACATCCCGACGGAG                                <  1:718061/76‑1                                             CCAAACAGAATAATCGCCAGCGTCGCCAGCAGTTGCTGAATCAGAATTAACGGATCAAACAACATCCCGACGGAGA                               >  1:711965/1‑76                                             CCAAACAGAATAATCGCCAGCGTCGCCAGCAGTTGCTGAATCAGAATTAACGGATCAAACAACATCCCGACGGAGA                               <  1:238360/76‑1                                              CAAACAGAATAATCGCCAGCGTCGCCAGCAGTTGCTGAATCAGAATTAACGGATCAAACAACATCCCGACGGAGAC                              >  1:1808985/1‑76                                              CAAACAGAATAATCGCCAGCGTCGCCAGCAGTTGCTGAATCAGAATTAACGGATCAAACAACATCCCGACGGAGAC                              >  1:188826/1‑76                                               AAACAGAATAATCGCCAGCGTCGCCAGCAGTTGCTGAATCAGAATTAACGGATCAAACAACATCCCGACGGAGACA                             <  2:1641600/76‑1                                                 ACAGAATAATCGCCAGCGTCGCCAGCAGTTGCTGAATCAGAATTAACGGATCAAACAACATCCCGACGGAGACAAA                           <  1:1882056/76‑1                                                  CAGAATAATCGCCAGCGTCGCCAGCAGTTGCTGAATCAGAATTAACGGATCAAACAACATCCCGACGGAGACAAAA                          >  1:1834388/1‑76                                                    GAATAATCGCCAGCGTCGCCAGCAGTTGCTGAATCAGAATTAACGGATCAAACAACATCCCGACGGAGACAAAAAA                        >  1:503645/1‑76                                                      ATAATCGCCAGCGTCGCCAGCAGTTGCTGAATCAGAATTAACGGATCAAACAACATCCCGACGGAGACAAAAAACA                      >  2:1062645/1‑76                                                         ATCGCCAGCGTCGCCAGCAGTTGCTGAATCAGAATTAACGGATCAAACAACATCCCGACGGAGACAAAAAACAGCA                   >  2:168099/1‑76                                                          TCGCCAGCGTCGCCAGCAGTTGCTGAATCAGAATTAACGGATCAAACAACATCCCGACGGAGACAAAAAACAGCAC                  <  2:810500/76‑1                                                           CGCCAGCGTCGCCAGCAGTTGCTGAATCAGAATTAACGGATCAAACAACATCCCGACGGAGACAAAAAACAGCACC                 >  1:357667/1‑76                                                                GCGTCGCCAGCAGTTGCTGAATCAGAATTAACGGATCAAACAACATCCCGACGGAGACAAAAAACAGCACCGCAAA            <  1:54032/76‑1                                                                GCGTCGCCAGCAGTTGCTGAATCAGAATTAACGGATCAAACAACATCCCGACGGAGACAAAAAACAGCACCGCAAA            <  1:171967/76‑1                                                                  GTCGCCAGCAGTTGCTGAATCAGAATTAACGGATCAAACAACATCCCGACGGAGACAAAAAACAGCACCGCAAACG          <  1:1037140/76‑1                                                                  GTCGCCAGCAGTTGCTGAATCAGAATTAACGGATCAAACAACATCCCGACGGAGACAAAAAACAGCACCGCAAACG          >  1:1272957/1‑76                                                                    CGCCAGCAGTTGCTGAATCAGAATTAACGGATCAAACAACATCCCGACGGAGACAAAAAACAGCACCGCAAACGCG        >  1:841673/1‑76                                                                    CGCCAGCAGTTGCTGAATCAGAATTAACGGATCAAACAACATCCCGACGGAGACAAAAAACAGCACCGCAAACGCG        >  1:337031/1‑76                                                                    CGCCAGCAGTTGCTGAATCAGAATTAACGGATCAAACAACATCCCGACGGAGACAAAAAACAGCACCGCAAACGCG        >  1:205054/1‑76                                                                    CGCCAGCAGTTGCTGAATCAGAATTAACGGATCAAACAACATCCCGACGGAGACAAAAAACAGCACCGCAAACGCG        >  1:1605408/1‑76                                                                     GCCAGCAGTTGCTGAATCAGAATTAACGGATCAAACAACATCCCGACGGAGACAAAAAACAGCACCGCAAACGCGT       >  2:1222166/1‑76                                                                     GCCAGCAGTTGCTGAATCAGAATTAACGGATCAAACAACATCCCGACGGAGACAAAAAACAGCACCGCAAACGCGT       <  1:1286176/76‑1                                                                     GCCAGCAGTTGCTGAATCAGAATTAACGGATCAAACAACATCCCGACGGAGACAAAAAACAGCACCGCAAACGCGT       >  1:1165774/1‑76                                                                       CAGCAGTTGCTGAATCAGAATTAACGGATCAAACAACATCCCGACGGAGACAAAAAACAGCACCGCAAACGCGTCG     >  2:829038/1‑76                                                                          CAGTTGCTGAATCAGAATTAACGGATCAAACAACATCCCGACGGAGACAAAAAACAGCACCGCAAACGCGTCGCGC  >  1:903838/1‑76                                                                                                                                                       AGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAGCGTCGCCAGCA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  REL606/474318‑474392 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GTTGCTGAATCAGAATTAACGGATCAAACAACATCCCGACGGAGACAAAAAACAGCACCGCAAACGCGTCGCGC  >  REL606/474402‑474475

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.