New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	REL606	2040311 =	67 (1.120)	5 (0.090)	5/140	6.8	7.6%	coding (1073/1113 nt)	vioA	VioA, involved in dTDP‑N‑acetylviosamine synthesis
	?	REL606	2040458 =	59 (1.050)					coding (926/1113 nt)	vioA	VioA, involved in dTDP‑N‑acetylviosamine synthesis
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

GTTGATAATCTACCAATAGCCAATAGTGTTGCTGATAAAGTCCTATGCTTGCCAATATATACAGATTTAAATGAAGAGAT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  REL606/2040390‑2040311 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑gagatAGGATATTATTTTTCTTAAGTAATTCATATGCCTGATCACGACTCATATGAAAACCATCATCTATGAGTATAGGA  >  REL606/2040458‑2040532                                                                                                                                                             GTTGATAATCTACCAATAGCCAATAGTGTTGCTGATAAAGTCCTATGCTTGCCAATATATACAGATTTAAATGAAG                                                                                 <  1:1467205/76‑1     ATAATCTACCAATAGCCAATAGTGTTGCTGATAAAGTCCTATGCTTGCCAATATATACAGATTTAAATGAAGAGAT                                                                             >  2:641984/1‑76               AATAGCCAATAGTGTTGCTGATAAAGTCCTATGCTTGCCAATATATACAGATTTAAATGAAGAGATAGGATATTAT                                                                   <  2:1353301/76‑1                      AATAGTGTTGCTGATAAAGTCCTATGCTTGCCAATATATACAGATTTAAATGAAGAGATAGGATATTATTTTTCTT                                                            <  2:495158/76‑1                       ATAGTGTTGCTGATAAAGTCCTATGCTTGCCAATATATACAGATTTAAATGAAGAGATAGGATATTATTTTTCTTA                                                           <  2:869610/76‑1                           TGTTGCTGATAAAGTCCTATGCTTGCCAATATATACAGATTTAAATGAAGAGATAGGATATTATTTTTCTTAAGTA                                                       <  2:1355230/76‑1                                   ATAAAGTCCTATGCTTGCCAATATATACAGATTTAAATGAAGAGATAGGATATTATTTTTCTTAAGTAATTCATAT                                               <  2:904453/76‑1                                                                                TAGGATATTATTTTTCTTAAGTAATTCATATGCCTGATCACGACTCATATGAAAACCATCATCTATGAGTATAGGA  >  1:509989/1‑76                                                                                TAGGATATTATTTTTCTTAAGTAATTCATATGCCTGATCACGACTCATATGAAAACCATCATCTATGAGTATAGGA  >  1:509992/1‑76                                                                                TAGGATATTATTTTTCTTAAGTAATTCATATGCCTGATCACGACTCATATGAAAACCATCATCTATGAGTATAGGA  <  1:629853/76‑1                                                                                TAGGATATTATTTTTCTTAAGTAATTCATATGCCTGATCACGACTCATATGAAAACCATCATCTATGAGTATAGGA  <  1:977909/76‑1                                                                                                                                                             GTTGATAATCTACCAATAGCCAATAGTGTTGCTGATAAAGTCCTATGCTTGCCAATATATACAGATTTAAATGAAGAGAT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  REL606/2040390‑2040311 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑gagatAGGATATTATTTTTCTTAAGTAATTCATATGCCTGATCACGACTCATATGAAAACCATCATCTATGAGTATAGGA  >  REL606/2040458‑2040532

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.