Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*REL606716,8320TG27.5% 103.7 / 17.6 51A31A (GCA→GCC) ECB_00663hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (7/30);  new base G (14/0);  total (21/30)
Fisher's exact test for biased strand distribution p-value = 9.00e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

GAAAAGAATGAACATTAGATACGCGATGCGGCGGCAGGTTACACGGTTATCACTACGATAGCCAATACTGGCTGGTGCATTACCATCGTGAACAATAGGACGCCGTACTGCTGGAAATTTCTTACCTTTATGACTCCCCGCGCCGCCGTAT  >  REL606/716757‑716907
                                                                           |                                                                           
gAAAAGAATGAACATTAGATACGCGATGCGGCGGCAGGTTACACGGTTATCACTACGATAGCCAATACTGGCTGGt                                                                             <  2:767855/76‑1 (MQ=255)
 aaaaGAATGAACATTAGATACGCGATGCGGCGGCAGGTTACACGGTTATCACTACGATAGCCAATACTGGCTGGTg                                                                            <  1:1143393/76‑1 (MQ=255)
      aaTGAACATTAGATACGCGATGCGGCGGCAGGTTACACGGTTATCACTACGATAGCCAATACTGGCTGGTGCATTa                                                                       <  1:1293325/76‑1 (MQ=255)
       aTGAACATTAGATACGCGATGCGGCGGCAGGTTACACGGTTATCACTACGATAGCCAATACTGGCTGGTGCATTAc                                                                      <  2:893755/76‑1 (MQ=255)
         gAACATTAGAGACGCGATGCGGCGGCAGGTTACACGGTTATCACTACGATAGCCAATACTGGCTGGTGCATTACCa                                                                    <  2:578193/76‑1 (MQ=255)
          aaCATTAAATACGCGATGCGGCGGCAGGTTACACGGTTATCACTACGATAGCCAATACTGGCTGGTGCATTACCAt                                                                   <  2:1380062/76‑1 (MQ=255)
            cATTAGATACGCGATGCGGCGGCAGGTTACACGGTTATCACTACGATAGCCAATACTGGCTGGGGGATTATCAACg                                                                 >  2:345780/1‑76 (MQ=255)
             aTTAGATACGCGATGCGGCGGCAGGTTACACGGTTATCACTACGATAGCCAATACTGGCTGGTGCATTACCATCGt                                                                <  2:282028/76‑1 (MQ=255)
             aTTAGATACGCGATGCGGCGGCAGGTTACACGGTTATCACTACGATAGCCAATACTGGCTGGGGGCTTACCATCGt                                                                >  2:610811/1‑76 (MQ=255)
              ttAGATACGCGATGCGGCGGCAGGTTACACGGTTATCACTACGATAGCCAATACTGGCTGGGGGATTTCCATCgcg                                                               >  2:833107/1‑74 (MQ=255)
              ttAGATACGCGATGCGGCGGCAGGTTACACGGTTATCACTACGATAGCCAATACTGGCTGGGGGATTACCATCggg                                                               >  2:959635/1‑74 (MQ=255)
                 gATACGCGATGCGGCGGCAGGTTACACGGTTATCACTACGATAGCCAATACTGGCTGGTGCATTACCATCGTGAAc                                                            <  2:833142/76‑1 (MQ=255)
                  aaaCGCGATGCGGCGGCAGGTTACACGGTTATCACTACGATAGCCAATACTGGCTGGGGGATTAACATCCTGGACa                                                           >  1:551626/3‑76 (MQ=255)
                  aTACGCGATGCGGCGGCAGGTTACACGGTTATCACTACGATAGCCAATACTGGCTGGGGCATTTCCATCGTGAACa                                                           >  1:551631/1‑76 (MQ=255)
                    aCGCGATGCGGCGGCAGGTTACACGGTTATCACTACGATAGCCAATACTGGCTGGTGCATTACCATCGTGAACAAt                                                         <  1:1501141/76‑1 (MQ=255)
                      gcgATGCGGCGGCAGGTTACACGGTTATCACTACGATAGCCAATACTGGCTGGTGCATTACCATCGTGAACAATAg                                                       <  2:1101342/76‑1 (MQ=255)
                      gcgATGCGGCGGCAGGTTACACGGTTATCACTACGATAGCCAATACTGGCTGGTGCATTACCATCGTGAACAATAg                                                       <  2:734557/76‑1 (MQ=255)
                       cgATGCGGCGGCAGGTTACACGGTTATCACTACGATAGCCAATACTGGCTGGGGGCTTACCATCGTGGACAATaag                                                      >  2:364764/1‑74 (MQ=255)
                       cgATGCGGCGGCAGGTTACACGGTTATCACTACGATAGCCAATACTGGCTGGGGCATTACCATCGTGAAAAATAgg                                                      >  2:1866296/1‑76 (MQ=255)
                        gATTCGGCGGCAGGTTACACGGTTATCACTACGATAGCCAATACTGGCTGGTGCATTACCATCGTGAACAATAGGa                                                     <  1:200436/76‑1 (MQ=255)
                        gATGCGGCGGCAGGTTACACGGTTATCACTACGATAGCCAATACTGGCTGGGGGCTTACCATCCTGGACAAAAggg                                                     >  2:1367925/1‑75 (MQ=255)
                         aTGCGGCGGCAGGTTACACGGTTATCACTACGATAGCCAATACTGGCTGGGGCATTACCATCGTGAACAATAGGAc                                                    >  1:1258683/1‑76 (MQ=255)
                          tGCGGCGGCAGGTTACACGGTTATCACTACGATAGCCAATACTGGCTGGTGCATTACCATCGTGAACAATAGGACg                                                   <  2:1491824/76‑1 (MQ=255)
                               cggcAGGTTACACGGTTATCACTACGATAGCCAATACTGGCTGGTGCATTACCATCGTGAACAATAGGACGCCGTa                                              <  2:455118/76‑1 (MQ=255)
                                ggcAGGTTACACGGTTATCACTACGATAGCCAATACTGGCTGGGGGATTACCATCGTGGACAATAGGACGCCGTAc                                             >  2:254818/1‑76 (MQ=255)
                                  cAGGTTACACGGTTATCACTACGATAGCCAATACTGGCTGGGGCATTACCATCGTGAACAATAGGACGCCGTActg                                           >  2:1336817/1‑76 (MQ=255)
                                    ggTTACACGGTTATCACTACGATAGCCAATACTGGCTGGTGCATTACCATCGTGAACAATAGGACGCCGTActgct                                         <  1:959635/76‑1 (MQ=255)
                                       tACACGGTTCTCACTACGATAGCCAATACTGGCTGGTGCATTACCATCGTGAACAATAGGACGCCGTACTGCTGGa                                      >  1:39092/1‑76 (MQ=255)
                                       tACACGGTTATCACTACGATAGCCAATACTGGCTGGTGCATTACCATCGTGAACAATAGGACGCCGTACTGCTGGa                                      >  2:1597560/1‑76 (MQ=255)
                                       tACACGGTTATCACTACGATAGCCAATACTGGCTGGGGGATTACCATCGTGAACAAAAGGACGCCGTACTGCTGGa                                      >  1:32428/1‑76 (MQ=255)
                                        acagGGTTATCACTACGATAGCCAATACTGGCTGGTGCATTACCATCGTGAACAATAGGACGCCGTACTGCTGGaa                                     <  2:1617910/76‑1 (MQ=255)
                                         cacGGTTATCACTACGATAGCCAATACTGGCTGGTGCATTACCATCGTGAACAATAGGACGCCGTActgctg                                        <  2:790215/72‑1 (MQ=255)
                                         cacGGTTATCACTACGATAGCCAATACTGGCTGGGGGATTACCATCGTGAACAATAGGACGCCGGActgctg                                        >  1:790215/1‑72 (MQ=255)
                                         cacGGTTATCACTACGATAGCCAATACTGGCTGGGGCATTACCATCGTGAACAATAGGACGCCGTACTGCTGGcaa                                    >  2:578421/1‑76 (MQ=255)
                                          acGGTTATCACTACGATAGCCAATACTGGCTGGGGCATTACCCTCCGTAACAATTAGAAGCCGTACCGCTGGCAAt                                   >  1:1194131/1‑76 (MQ=255)
                                            ggTTATCACTACGATAGCCAATACTGGCTGGGGCATTACCATCGTGAACAATAGGACGCCGTACTGCTGGGAAttt                                 >  1:1611855/1‑76 (MQ=255)
                                               tATCACTACGATAGCCAATACTGGCTGGTGCATTACCATCGTGAACAATAGGACGCCGTACTGCTGGGAATTTCtt                              >  1:506953/1‑76 (MQ=255)
                                                 tCACTACGATAGCCAATACTGGCTGGTGCATTACCATCGTGAACAATAGGACGCCGTACTGCTGGAAATTTCTTAc                            <  1:345780/76‑1 (MQ=255)
                                                   aCTACGATAGCCAATACTGGCTGGTGCATTACCATCGTGAACAATAGGACGCCGTACTGCTGGAAATTTTTTACCt                          >  1:1513989/1‑76 (MQ=255)
                                                    cTACGATAGCCAATACTGGCTGGTGCATTACCATCGTGAACAATAGGACGCCGTACTGCTGGAAATTTCTTACCtt                         <  2:372922/76‑1 (MQ=255)
                                                     tACGATAGCCAATACTGGCTGGTGCATTACCATCGTGAACAATAGGACGCCGTACTGCTGGAAATTTCTTACCttt                        <  2:1805506/76‑1 (MQ=255)
                                                     tACGATAGCCAATACTGGCTGGTGCATTACCATCGTGAACAATAGGACGCCGTACTGCTGGAAATTTCTTACCttt                        <  1:1731506/76‑1 (MQ=255)
                                                      aCGATAGCCAATACTGGCTGGTGCATTACCATCGTGAACAATAGGACGCCGTACTGCTGGAAATTTCTTACCTTTa                       <  2:614552/76‑1 (MQ=255)
                                                      aCGATAGCCAATACTGGCTGGTGCATTACCATCGTGAACAATAGGACGCCGTACTGCTGGAAATTTCTTACCTTTa                       <  1:1469966/76‑1 (MQ=255)
                                                      aCGATAGCCAATACTGGCTGGTGCATTACCATCGTGAACAATAGGACGCCGTACTGCTGGAAATTTCTTACCTTTa                       <  2:829356/76‑1 (MQ=255)
                                                       cGATAGCCAATACTGGCTGGTGCATTACCATCGTGAACAATAGGACGCCGTACTGCTGGAAATTTCTTACCTTTAt                      <  2:1371786/76‑1 (MQ=255)
                                                       cGATAGCCAATACTGGCTGGTGCATTACCATCGTGAACAATAGGACGCCGTACTGCTGGAAATTTCTTACCTTTAt                      <  1:1490680/76‑1 (MQ=255)
                                                          tAGCCAATACTGGCTGGGGGATTACCATCGTGAACAATAGGACGCCGTACTGCTGGGAATTTCTTACCTTTTTGat                   >  2:116821/1‑75 (MQ=255)
                                                          tAGCCAATACTGGCTGGGGCATTACCATCGTGAACAATAGGACGCCGTACTGCTGGGAATTTCTTACCTTTATGAc                   >  2:1410013/1‑76 (MQ=255)
                                                           aGCCAATACTGGCTGGTGCATTACCATCGTGAACAATAGGACGCCGTACTGCTGGAAATTTCTTACCTTTATGACt                  <  1:654282/76‑1 (MQ=255)
                                                              cAATACTGGCTGGTGCATTACCATCGTGAACAATAGGACGCCGTACTGCTGGAAATTTCTTACCTTTATGACTccc               <  1:1369146/76‑1 (MQ=255)
                                                               aaTACTGGCTGGTGCATTACCATCGTGAACAATAGGACGCCGTACTGCTGGAAATTTCTTACCTTTATGACTcccc              <  1:53419/76‑1 (MQ=255)
                                                                 tACTGGCTGGTGCATTACCATCGTGAACAATAGGACGCCGTACTGCTGGAAATTTCTTACCTTTATGACTcccccc            >  2:20300/1‑74 (MQ=255)
                                                                    tggctggTGCATTACCATCGTGAACAATAGGACGCCGTACTGCTGGAAATTTCTTACCTTTATGACTCCCCGcgcc         >  2:1667167/1‑76 (MQ=255)
                                                                       ctggTGCATTACCATCGTGAACAATAGGACGCCGTACTGCTGGAAATTTCTTACCTTTATGACTCCCCGCgccgcc      <  1:666771/76‑1 (MQ=255)
                                                                       ctggTGCATTACCATCGTGAACAATAGGACGCCGTACTGCTGGAAATTTCTTACCTTTATGACTCCCCGCgccgcc      >  2:1550646/1‑76 (MQ=255)
                                                                        tggTGCATTACCATCGTGAACAATAGGACGCCGTACTGCTGGAAATTTCTTACCTTTATGACTCCCCGCGccgccg     >  1:774266/1‑76 (MQ=255)
                                                                         ggTGCATTACCATCGTGAACAATAGGACGCCGTACTGCTGGAAATTTCTTCCCTTTATGACTCCCCGCGCCGCCGt    <  2:1790758/76‑1 (MQ=255)
                                                                           tGCATTACCATCGTGAACAATAGGACGCCGTACTGCTGGAAATTTCTTACCTTTATGACTCCCCGCGCCGCCGTAt  <  1:1088083/76‑1 (MQ=255)
                                                                           tGCATTACCATCGTGAACAATAGGACGCCGTACTGCTGGAAATTTCTTACCTTTATGACTCCCCGCGCCGCCGTAt  <  2:53514/76‑1 (MQ=255)
                                                                           tGCATTACCATCGTGAACAATAGGACGCCGTACTGCTGGAAATTTCTTACCTTTATGACTCCCCGCGCCGCCGTAt  <  2:32428/76‑1 (MQ=255)
                                                                           tGCATTACCATCGTGAACAATAGGACGCCGTACTGCTGGAAATTTCTTACCTTTATGACTCCCCGCGCCGCCGTAt  >  2:1462956/1‑76 (MQ=255)
                                                                           |                                                                           
GAAAAGAATGAACATTAGATACGCGATGCGGCGGCAGGTTACACGGTTATCACTACGATAGCCAATACTGGCTGGTGCATTACCATCGTGAACAATAGGACGCCGTACTGCTGGAAATTTCTTACCTTTATGACTCCCCGCGCCGCCGTAT  >  REL606/716757‑716907

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑