| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 1,688,990 | 0 | A | C | 42.4% | 39.5 / 13.5 | 33 | T92P (ACC→CCC) | nth | DNA glycosylase and apyrimidinic (AP) lyase (endonuclease III) |
| Reads supporting (aligned to +/- strand): ref base A (14/5); new base C (0/14); total (14/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.45e-05 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CTGGGCGTTGAAGGGGTGAAAACCTATATCAAAACGATTGGGCTTTATAACAGCAAAGCAGAAAATATCATCAAAACCTGCCGTATCTTGCTGGAGCAGCATAATGGCGAGGTTCCGGAAGATCGTGCTGCGCTTGAAGCCCTGCCCGG > REL606/1688915‑1689063 | cTGGGCGTTGAAGGGGTGAAAACCTATATCAAAACGATTGGGCTTTATAACAGCAAAGCAGAAAATATCATCaaaa < 2:983900/76‑1 (MQ=255) ggCGTTGAAGGGGTTAAAACCTATATCAAAACGATTGGGCTTTATAACAGCAAAGCAGAAAATATCATCAAAACCt < 1:934680/76‑1 (MQ=255) ggCGTTGAAGGGGTGAAAACCTATATCAAAACGATTGGGCTTTATAACAGCAAAGCAGAAAATATCATCAAAACCt < 1:240545/76‑1 (MQ=255) aaGGGGTGAAAACCTATATCAAAACGATTGGGCTTTATAACAGCAAAGCAGAAAATATCATCAAAACCTGCCGTAt < 2:600301/76‑1 (MQ=255) aaGGGGTGAAAACCTATATCAAAACGATTGGGCTTTATAACAGCAAAGCAGAAAATATCATCAAAACCTGCCGTAt < 1:385953/76‑1 (MQ=255) gggTGAAAACCTATATCAAAACGATTGGGCTTTATAACAGCAAAGCAGAAAATATCATCAAACCCTGCCGTATCtt < 2:109468/76‑1 (MQ=255) aCCTATATCAAAACGATGGGGCTTTATAACAGAAAAGCAAAAAATATCATCAAACCCTGCCGTATCTTGCTGGagc < 2:260090/76‑1 (MQ=255) ccTATAACAAAACGATTGGGCTTTATAACAGAAAAGAAAAAAATATCATCAAAACCTGCCGTATCTTGCTGGagca < 1:1319261/76‑1 (MQ=255) tataTCAAAACGATTGGGCTTTATAACAGCAAAGCAGAAAATATCATCAAAACCTGCCGTATCTTGCTGGagcagc > 2:957351/1‑76 (MQ=255) atatCAAAACGATTGGGCTTTATAACAGCAAAGCAGAAAATATCATCAAAACCTGCCGTATCTTGCTGGAgcagca > 1:1358375/1‑76 (MQ=255) atCAAAACGATTGGGCTTTATAACAGCAAAGCAGAAAATATCATCAAAACCTGCCGTATCTTGCTGGAGCAGCATa > 1:187462/1‑76 (MQ=255) atCAAAACGATTGGGCTTTATAACAGCAAAGCAGAAAATATCATCAAAACCTGCCGTATCTTGCTGGAGCAGCATa > 1:187479/1‑76 (MQ=255) aaaaaGGATTGGGCTTTATAACAGCAAAGCAAAAAATATCATCAAACCCTGCCGTATCTTGCTGGAGCAGCATAAt < 1:661754/75‑1 (MQ=255) aaaCGATTGGGCTTTATAACAGCAAAGCAGAAAATATCATCAAAACCTGCCGTATCTTGCTGGAGCAGCATAATgg > 1:1699592/1‑76 (MQ=255) gATTGGGCTTTATAACAGCAAAGAAGAAAATATCATCAAAACCTGCCGTATCTTGCTGGAGCAGCATAATGGCGAg < 1:1768800/76‑1 (MQ=255) gATTGGGCTTTAAAACAGAAAAGCAGAAAATATCATCAAACCCTGCCGTATCTTGCTGGAGCAGCATAATGGCGAg < 1:206330/76‑1 (MQ=255) gATGGGGCTTTATAACAGCAAAGCAGAAAATATCATCAAACCCTGCCGTATCTTGCTGGAGCAGCATAATGGCGAg < 1:477729/76‑1 (MQ=255) ttGGGCTTTATAACAGCAAAGCAGAAAATATCATCAAAACCTGCCGTATCTTGCTGGAGCAGCATAATGGCGAGGt > 1:1418252/1‑76 (MQ=255) gggCTTTATAACAGCAAAGCAGAAAATATCATCAAAACCTGCCGTATCTTGCTGGAGCAGCATAATGGCGAGGTTc > 1:676919/1‑76 (MQ=255) cTTTATAACAGAAAAGCAAAAAATATCATCAAACCCTGCCGTATCTTGCTGGAGCAGCATAATGGCGAGGTTCCgg < 2:832083/76‑1 (MQ=255) tttATAACAGAAAAGCAAAAAATATCATCAAAACCTGCCGTATCTTGCTGGAGCAGCATAATGGCGAGGTTCCGGa < 1:1657216/76‑1 (MQ=255) ataACAGCAAAGCAGAAAATATCATCAAAACCTGCCGTATCTTGCTGGAGCAGCATAATGGCGAGGTTCCGGAAGa > 2:830633/1‑76 (MQ=255) ataACAGCAAAGCAAAAAATATCATCAAACCCTGCCGTATCTTGCTGGAGCAGCATAATGGCGAGGTTCCGGAAGa < 2:880188/76‑1 (MQ=255) taACAGCAAAGCAGAAAATATCATCAAAACCTGCCGTATCTTGCTGGAGCAGCATAATGGCGAGGTTCCGGAAGAt > 1:1703032/1‑76 (MQ=255) taACAGCAAAGCAGAAAATATCATCAAAACCTGCCGTATCTTGCTGGAGCAGCATAATGGCGAGGTTCCGGAAGAt > 2:1524273/1‑76 (MQ=255) aaCAGCAAAGCAGAAAATATCATCAAACCCTGCCGTATCTTGCTGGAGCAGCATAATGGCGAGGTTCCGGAAGATc < 1:1664603/76‑1 (MQ=255) aCAGCAAAGCAAAAAATATCATCAAACCCTGCCGTATCTTGCTGGAGCAGCATAATGGCGAGGTTCCGGAAGATCg < 1:1358492/76‑1 (MQ=255) cAGAAAAGCAAAAAATATCATCAAACCCTGCCGTATCTTGCTGGAGCAGCATAATGGCGAGGTTCCGGAAGATCGt < 1:1162070/76‑1 (MQ=255) agaaaaGCAAAAAATCTCACCAAACCCTGCCGTATCTTGCTGGAGCAGCATAATGGCGAGGTTCCGGAAGATCGtg < 2:897376/73‑1 (MQ=255) aGCAAAGCAGAAAATATCATCAAAACCTGCCGTATCTTGCTGGAGCAGCATAATGGCGAGGTTCCGGAAGATCGtg > 1:440449/1‑76 (MQ=255) aaaGCAGAAAATACCATCAAACCCTGCCGTATCTTGCTGGAGCAGCATAATGGCGAGGTTCCGGAAGATCGtgctg < 2:562225/76‑1 (MQ=255) aaGCAGAAAATATCATCAAAACCTGCCGTATCTTGCTGGAGCAGCATAATGGCGAGGTTCCGGAAGATCGtgctgc > 2:1113101/1‑76 (MQ=255) aaaaaaTATCATCAAACCCTGCCGTATCTTGCTGGAGCAGCATAATGGCGAGGTTCCGGAAGATCGTGCTGCGCtt < 2:357288/74‑1 (MQ=255) aaaaaaTATCATCAAACCCTGCCGTATCTTGCTGGAGCAGCATAATGGCGAGGTTCCGGAAGATCGTGCTGCGCtt < 2:1444189/74‑1 (MQ=255) aaaaaaTATCATCAAACCCTGCCGTATCTTGCTGGAGCAGCATAATGGCGAGGTTCCGGAAGATCGTGCTGCGCtt < 2:1321940/74‑1 (MQ=255) tcatcaAAACCTGCCGTATCTTGCTGGAGCAGCATAATGGCGAGGTTCCGGAAGATCGTGCTGCGCTTGAAGCCCt > 2:779511/1‑76 (MQ=255) tcatcaAAACCTGCCGTATCTTGCTGGAGCAGCATAATGGCGAGGTTCCGGAAGATCGTGCTGCGCTTGAAGCCCt > 2:403771/1‑76 (MQ=255) tcaAAACCTGCCGTATCTTGCTGGAGCAGCATAATGGCGAGGTTCCGGAAGATCGTGCTGCGCTTGAAGCCCTGcc > 2:831150/1‑76 (MQ=255) aaaCCTGCCGTATCTTGCTGGAGCAGCATAATGGCGAGGTTCCGGAAGATCGTGCTGCGCTTGAAGCCCTGCCCgg > 2:704346/1‑76 (MQ=255) aaaCCTGCCGTATCTTGCTGGAGCAGCATAATGGCGAGGTTCCGGAAGATCGTGCTGCGCTTGAAGCCCTGCCCgg > 2:263855/1‑76 (MQ=255) | CTGGGCGTTGAAGGGGTGAAAACCTATATCAAAACGATTGGGCTTTATAACAGCAAAGCAGAAAATATCATCAAAACCTGCCGTATCTTGCTGGAGCAGCATAATGGCGAGGTTCCGGAAGATCGTGCTGCGCTTGAAGCCCTGCCCGG > REL606/1688915‑1689063 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |