Predicted mutation
evidence seq id position mutation annotation gene description
RA REL606 3,613,023 A→C intergenic (‑84/+99) dctA ← / ← yhjK C4‑dicarboxylate transport protein/predicted diguanylate cyclase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*REL6063,613,0230AC93.4% 313.6 / 6.8 76intergenic (‑84/+99)dctA/yhjKC4‑dicarboxylate transport protein/predicted diguanylate cyclase
Reads supporting (aligned to +/- strand):  ref base A (1/4);  new base C (32/39);  total (33/43)
Fisher's exact test for biased strand distribution p-value = 3.81e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01

AGTAAAACCACAGATCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACTTTTT  >  REL606/3612952‑3613095
                                                                       |                                                                        
aGTAAAACCACAGATCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGCAATg                                                                      <  1:1764930/76‑1 (MQ=255)
aGTAAAACCACAGATCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGCAATg                                                                      <  1:1764914/76‑1 (MQ=255)
aGTAAAACCACAGATCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATg                                                                      <  1:493924/76‑1 (MQ=255)
aGTAAAACCACAGATCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATg                                                                      >  2:1162372/1‑76 (MQ=255)
 gTAAAACCACAGATCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGCAATGt                                                                     >  1:147808/1‑76 (MQ=255)
   aaaaCCACAGATCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTAc                                                                   <  1:64517/76‑1 (MQ=255)
      aCCACAGATCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGCAATGTACCTg                                                                >  1:391142/1‑76 (MQ=255)
      aCCACAGATCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGCAATGTACCTg                                                                >  1:892851/1‑76 (MQ=255)
       ccACAGATCTTGTAAGGTTATGGGGTACCTGCGGCTTTAAAATAACACCCAGACAACATCACAGCAATGTACCTgg                                                               <  1:108004/76‑1 (MQ=255)
       ccACAGATCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGCAATGTACCTgg                                                               <  1:370812/76‑1 (MQ=255)
         acaGATCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGCAATGTACCTGGAt                                                             <  1:1364293/76‑1 (MQ=255)
         acaGATCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGCAATGTACCTGGAt                                                             >  2:1668303/1‑76 (MQ=255)
          caGATCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGCAATGTACCTGGATc                                                            >  1:1890498/1‑76 (MQ=255)
           aGATCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGCAATGTACCTGGATCa                                                           <  2:1873653/76‑1 (MQ=255)
           aGATCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGCAATGTACCTGGATCa                                                           >  2:615453/1‑76 (MQ=255)
            gATCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGCAATGTACCTGGATCAt                                                          <  1:1450/76‑1 (MQ=255)
            gATCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGCAATGTACCTGGATCAt                                                          <  1:1483471/76‑1 (MQ=255)
            gATCTTGTAAGATTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCAt                                                          <  2:401215/76‑1 (MQ=255)
              tCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGCAATGTACCTGGATCATaa                                                        >  1:351211/1‑76 (MQ=255)
               cTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGCAATGTACCTGGATCATAAt                                                       <  1:896854/76‑1 (MQ=255)
                ttGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGCAATGTACCTGGATCATAAtt                                                      <  2:1363176/76‑1 (MQ=255)
                ttGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGCAATGTACCTGGATCATAAtt                                                      <  2:521133/76‑1 (MQ=255)
                 tGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGCAATGTACCTGGATCATAATTg                                                     >  1:695396/1‑76 (MQ=255)
                 tGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGCAATGTACCTGGATCATAATTg                                                     <  2:970058/76‑1 (MQ=255)
                 tGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGCAATGTACCTGGATCATAATTg                                                     <  2:970073/76‑1 (MQ=255)
                  gTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGCAATGTACCTGGATCATAATTGa                                                    <  2:808241/76‑1 (MQ=255)
                  gTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGCAATGTACCTGGATCATAATTGa                                                    >  2:478477/1‑76 (MQ=255)
                    aaGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGCAATGTACCTGGATCATAATTGaaa                                                  >  2:307449/1‑76 (MQ=255)
                     aGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGCAATGTACCTGGATCATAATTGAAAc                                                 >  2:333181/1‑76 (MQ=255)
                      ggTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGCAATGTACCTGGATCATAATTGAAACa                                                <  1:1203561/76‑1 (MQ=255)
                       gTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGCAATGTACCTGGATCATAATTGAAACaa                                               >  2:1131731/1‑76 (MQ=255)
                        ttATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGCAATGTACCTGGATCATAATTGAAACaaa                                              >  1:407527/1‑76 (MQ=255)
                         tATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGCAATGTACCTGGATCATAATTAAAACaaaa                                             <  1:505582/76‑1 (MQ=255)
                           tGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGCAATGTACCTGGATCATAATTGAAACAAAAAg                                           >  1:1305352/1‑76 (MQ=255)
                            ggggTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGCAATGTACCTGGATCATAATTGAAACAAAAAgg                                          >  1:1452337/1‑76 (MQ=255)
                              ggTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGCAATGTACCTGGATCATAATTGAAACAAAAAGGtt                                        <  1:270538/76‑1 (MQ=255)
                              ggTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGtt                                        <  1:1162372/76‑1 (MQ=255)
                               gTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGCAATGTCCCTGGATCATAATTGAAACAAAAAGGTTa                                       <  2:1219260/76‑1 (MQ=255)
                               gTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGCAATGTACCTGGATCATAATTGAAACAAAAAGGTTa                                       >  2:775776/1‑76 (MQ=255)
                                tACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGCAATGTACCTGGATCATAATTGAAACAAAAAGGTTaa                                      <  2:1458420/76‑1 (MQ=255)
                                 aCCTGCGGCCTTAAAATAACACCCAGACAACATCACAGCAATGTACCTGGATCATAATTGAAACAAAAAGGTTaaa                                     >  2:1743435/1‑76 (MQ=255)
                                   cTGCGGCCTTAAAATAACACCCAGACAACATCACAGCAATGTACCTGGATCATAATTGAAACAAAAAGGTTaaaaa                                   >  2:627710/1‑76 (MQ=255)
                                      cGGCCTTAAAATAACACCCAGACAACATCACAGCAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGtt                                >  2:171916/1‑76 (MQ=255)
                                       ggCCTTAAAATAACACCCAGACAACATCACAGCAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGttt                               >  1:1266692/1‑76 (MQ=255)
                                       ggCCTTAAAATAACACACAGACAACATCACAGCAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGttt                               <  2:548621/76‑1 (MQ=255)
                                        gCCTTAAAATAACACCCAGACAACATCACAGCAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTg                              <  1:145029/76‑1 (MQ=255)
                                        gCCTTAAAATAACACCCAGACAACATCACAGCAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTg                              <  2:134789/76‑1 (MQ=255)
                                          cTTAAAATAACACCCAGACAACATCACAGCAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAg                            <  2:282207/76‑1 (MQ=255)
                                           ttAAAATAACACCCAGACAACATCACAGCAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGc                           >  1:13635/1‑76 (MQ=255)
                                            tAAAATAACACCCAGACAACATCACAGCAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCt                          <  2:311241/76‑1 (MQ=255)
                                            tAAAATAACACCCAGACAACATCACAGCAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCt                          >  1:705702/1‑76 (MQ=255)
                                             aaaaTAACACCCAGACAACATCACAGCAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTg                         <  2:1890498/76‑1 (MQ=255)
                                             aaaaTAACACCCAGACAACATCACAGCAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTg                         <  1:855738/76‑1 (MQ=255)
                                              aaaTAACACCCAGACAACATCACAGCAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTgg                        >  2:1374700/1‑76 (MQ=255)
                                              aaaTAACACCCAGACAACATCACAGCAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTgg                        <  2:1308598/76‑1 (MQ=255)
                                                 tAACACCCAGACAACATCACAGCAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTc                     >  1:1158791/1‑76 (MQ=255)
                                                     acCCAGACAACATCACAGCAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGcac                 <  1:1268630/76‑1 (MQ=255)
                                                     acCCAGACAACATCACAGCAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGcac                 <  1:27424/76‑1 (MQ=255)
                                                         aGACAACATCACAGCAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAAc             >  2:187858/1‑76 (MQ=255)
                                                          gACAACATCACAGCAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAAcc            <  2:161957/76‑1 (MQ=255)
                                                          gACAACATCACAGCAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAAcc            <  2:608701/76‑1 (MQ=255)
                                                          gACAACATCACAGCAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAAcc            <  2:1638115/76‑1 (MQ=255)
                                                           acaacaTCACAGCAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCa           <  2:584640/76‑1 (MQ=255)
                                                           acaacaTCACAGCAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCa           <  2:1869776/76‑1 (MQ=255)
                                                           acaacaTCACAGCAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCa           >  2:1456872/1‑76 (MQ=255)
                                                            caacaTCACAGCAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAg          <  1:1785958/76‑1 (MQ=255)
                                                            caacaTCACAGCAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAg          <  1:1825127/76‑1 (MQ=255)
                                                              acaTCACAGCAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCa        <  1:628511/76‑1 (MQ=255)
                                                              acaTCACAGCAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCa        >  1:1776228/1‑76 (MQ=255)
                                                               caTCACAGCAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCAc       >  2:1609846/1‑76 (MQ=255)
                                                               caTCACAGCAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCAc       >  2:661994/1‑76 (MQ=255)
                                                               caTCACAGCAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCAc       >  2:1558435/1‑76 (MQ=255)
                                                                  cacaGCAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACttt    <  1:1185365/76‑1 (MQ=255)
                                                                   acaGCAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACtttt   <  2:1750525/76‑1 (MQ=255)
                                                                    caGCAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACttttt  >  2:575984/1‑76 (MQ=255)
                                                                    caGCAATGTACCTGGATCATAATTCAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACttttt  >  1:1597173/1‑76 (MQ=255)
                                                                       |                                                                        
AGTAAAACCACAGATCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACTTTTT  >  REL606/3612952‑3613095

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: