| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 3,911,998 | C→A | intergenic (‑199/‑124) | yifB ← / → ilvL | predicted bifunctional enzyme and transcriptional regulator/ilvG operon leader peptide |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 3,911,998 | 0 | C | A | 94.7% | 202.6 / 3.5 | 57 | intergenic (‑199/‑124) | yifB/ilvL | predicted bifunctional enzyme and transcriptional regulator/ilvG operon leader peptide |
| Reads supporting (aligned to +/- strand): ref base C (1/2); new base A (22/32); total (23/34) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.69e-01 | |||||||||||
GAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTT‑AAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCAAAAAATATCTTGTACTATTTACAAAA > REL606/3911926‑3912069 | gAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTT‑Aaaaaaaaaa < 2:460513/76‑2 (MQ=255) aaCTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTT‑AAAAAaaaaca > 1:1873537/1‑76 (MQ=255) tACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTT‑AAAAAAAAACAAtt > 1:187527/1‑76 (MQ=255) aCGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTT‑AAAAAAAAACAAttt < 2:887557/76‑1 (MQ=255) aCGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTT‑AAAAAAAAACAAttt < 2:1690602/76‑1 (MQ=255) cGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTT‑AAAAAAAAACAATTta < 1:1818857/76‑1 (MQ=255) gAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTT‑AAAAAAAAACAATTtat < 1:1645556/76‑1 (MQ=255) ggAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTT‑AAAAAACAACAATTTatat > 2:3069/1‑76 (MQ=255) ggAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTT‑AAAAAAAAACAATTTATATTGaaa > 1:489222/1‑76 (MQ=255) gAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTT‑AAAAAAAAACAATTTATATTGAAat > 1:1330081/1‑76 (MQ=255) gAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTT‑AAAAAAAAACAATCTATATTGAAat < 2:1619792/76‑1 (MQ=255) aacaacATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTT‑AAAAAAAAACAATTTATATTGAAatt < 2:601718/76‑1 (MQ=255) aacaacATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTT‑AAAAAAAAACAATTTATATTGAAatt > 1:959467/1‑76 (MQ=255) aacaacATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTT‑AAAAAAAAACAATTTATATTGAAatt > 1:503111/1‑76 (MQ=255) acaacaTTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTT‑AAAAAACAACAATTTATATTGAAatta < 2:1137231/76‑1 (MQ=255) caacaTTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTT‑AAAAAAAAACAATTTATATTGAAattat > 2:1528670/1‑76 (MQ=255) aaacaTTCATACTGAAATTGATTTTTTTTCCCTCACTA‑TTTAATTTT‑AAAAAAAAACAATTTATATTGAAattatt < 1:1323288/75‑1 (MQ=255) aacaTTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTT‑AAAAAAAAACAATTTATATTGAAattatt > 1:1542511/1‑76 (MQ=255) aacaTTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTT‑AAAAAAAAACAATTTATATTGAAattatt > 2:35822/1‑76 (MQ=255) acaTTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTT‑AAAAAAAAACAATTTATATTGAAAttatta < 2:1162245/76‑1 (MQ=255) acaTTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTT‑AAAAAAAAACAATTTATATTGAAAttatta < 1:1444021/76‑1 (MQ=255) acaTTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTT‑AAAAAAAAACAATTTATATTGAAAttatta < 1:206944/76‑1 (MQ=255) acaTTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATCTTT‑AAAAAAAAACAATTTATATTGAAAttatta > 2:670332/1‑76 (MQ=255) caTTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTT‑AAAAAAAAACAATTTATATTGAAATTATTaa < 2:1845685/76‑1 (MQ=255) aTTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTT‑AAAAAAAAACAATTTATATTGAAATTATTaaa < 2:599099/76‑1 (MQ=255) aTTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTT‑AAAAAAAAACAATTTATATTGAAATTATTaaa < 2:338494/76‑1 (MQ=255) aTTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTT‑AAAAAAAAACAATTTATATTGAAATTATTaaa < 1:833301/76‑1 (MQ=255) aTTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTT‑AAAAAAAAACAATTTATATTGAAATTATTaaa < 2:599094/76‑1 (MQ=255) aTTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTT‑AAAAAAAAACAATTTATATTGAAAGTATTaaa < 2:153013/76‑1 (MQ=255) tACTGAAATTGAATTTTTTTGACTCACTATTTTATTTTT‑AAAAAAAAACAATTTATATTGAAATTATTAAACGcat < 2:1609168/76‑1 (MQ=255) tGAAATTGAATTTTTTTCACTCACTATTTTATTTTT‑AAAAAAAAACAATTTATATTGAAATTATTAAACGcatcat < 2:1499826/76‑1 (MQ=255) aaaTTGAATTTTTTTCACTCACTATTTTATTTTT‑AAAAAAAAACAATTTATATTGAAATTATTAAACGCATCATaa < 1:1428496/76‑1 (MQ=255) aaTTGAATTTTTTTCACTCACTATTTTATTTTT‑AAAAAAAAACAATTTATATTGAAATTATTAAACGCATCATaaa < 1:382436/76‑1 (MQ=255) ttGAATTTTTTTCACTCACTATTTTATTTTT‑AAAAAAAAACAATTTATATTGAAATTATTAAACGCATCATaatgg < 2:1326926/76‑4 (MQ=255) ttGAATTTTTTTCACTCACTATTTTATTTTT‑AAAAAAAAACAATTTATATTGAAATTATTAAACGCATCATaaaaa > 2:187012/1‑76 (MQ=255) ttGAATTTTTTTCACTCACTATTTTATTTTT‑AAAAAAAAACAATTTATATTGAAATTATTAAACGCATCATaaaaa > 2:417067/1‑76 (MQ=255) tcactcacTATTTTATTTTT‑AAAAAAAAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCaaaaa < 1:142570/76‑1 (MQ=255) actcactATTTTATTTTT‑AAAAAAAAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCaaaaact < 2:33064/76‑3 (MQ=255) ctcactATTTTATTTTT‑AAAAAAAAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCAAAAAata > 1:571610/1‑76 (MQ=255) ctcactATTTTATTTTT‑AAAAAAAAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCAAAAAata > 1:571591/1‑76 (MQ=255) cactATTTTATTTTT‑AAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCAAAAAATATc < 2:1144325/76‑1 (MQ=255) cactATTTTATTTTT‑AAAAAAAAACAATTTATATTGAAATTATTAAACGCATCATAAAGATCGGCCAAAAAATATc < 1:1580800/76‑1 (MQ=255) actATTTTATTTTT‑AAAAAAAAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCAAAAAATATCt > 2:657695/1‑76 (MQ=255) ctATTTTATTTTT‑AAAAAAAAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCAAAAAATATCtt < 2:148078/76‑1 (MQ=255) ttttattttT‑AAAAAAAAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCAAAAAATATCTTGTa > 1:1877665/1‑76 (MQ=255) ttttattttT‑AAAAAAAAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCAAAAAATATCTTGTa > 1:1686177/1‑76 (MQ=255) atttattttTAAAAAAAAAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCAAAAAATATCTTGt < 2:936867/75‑1 (MQ=255) tttattttT‑AAAAAAAAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCAAAAAATATCTTGTAc > 1:780452/1‑76 (MQ=255) tttattttT‑AAAAAAAAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCAAAAAATATCTTGTAc > 1:1592336/1‑76 (MQ=255) ttattttT‑AAAAAAAAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCAAAAAATATCTTGTACt > 2:487754/1‑76 (MQ=255) ttattttT‑AAAAAAAAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCAAAAAATATCTTGTACt > 2:487763/1‑76 (MQ=255) tattttT‑AAAAAAAAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCAAAAAATATCTTGTACTa < 1:54658/76‑1 (MQ=255) attttT‑AAAAAAAAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCAAAAAATATCTTGTACTAt < 1:1845425/76‑1 (MQ=255) ttttt‑AAAAAAAAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCAAAAAATATCTTGTACTAtt < 1:1692752/76‑1 (MQ=255) tttt‑AAAAAAAAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCAAAAAATATCTTGTACTAttt < 2:1785337/76‑1 (MQ=255) tttt‑AAAAAAAAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCAAAAAATATCTTGTACTAttt < 1:1292564/76‑1 (MQ=255) ttt‑AAAAAAAAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCAAAAAATATCTTGTACTATTTa > 1:1286123/1‑76 (MQ=255) ttt‑AAAAAAAAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCAAAAAATATCTTGTACTATTTa > 1:1480309/1‑76 (MQ=255) ttt‑AAAAAAAAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCAAAAAATATCTTGTACTATTTa < 1:1589961/76‑1 (MQ=255) tt‑AAAAAAAAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCAAAAAATATCTTGTACTATTTAc < 1:1345323/76‑1 (MQ=255) aaaaaaaaCAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCAAAAAATATCTTGTACTATTTACaaa > 1:1624284/1‑76 (MQ=255) aaaaaaaaCAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCAAAAAATATCTTGTACTATTTACaaa > 1:882634/1‑76 (MQ=255) aaaaaaaaCAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCAAAAAATATCTTGTACTATTTACaaa > 2:1112159/1‑76 (MQ=255) aaaaaaACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCAAAAAATATCTTGTACTATTTACaaaa > 2:1322677/1‑76 (MQ=255) | GAACTACGAGGAAGGGAACAACATTCATACTGAAATTGAATTTTTTTCACTCACTATTTTATTTTT‑AAAAAACAACAATTTATATTGAAATTATTAAACGCATCATAAAAATCGGCCAAAAAATATCTTGTACTATTTACAAAA > REL606/3911926‑3912069 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |