| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 3,268,816 | (A)8→7 | intergenic (‑131/‑128) | rplU ← / → ispB | 50S ribosomal protein L21/octaprenyl diphosphate synthase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 3,268,809 | 0 | A | . | 91.2% | 226.6 / 19.7 | 57 | intergenic (‑124/‑135) | rplU/ispB | 50S ribosomal protein L21/octaprenyl diphosphate synthase |
| Reads supporting (aligned to +/- strand): ref base A (2/3); new base . (18/34); total (20/37) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.23e-01 | |||||||||||
ATAAATATTCACAATAGGGCGCGAATATTACGCAAAACGCACGCCTTTGACAAGTGCTACAGTCAATACACGAAGAAAAAAAACACAACTTGTACGGTAACGTTTATCTGTGCCATTTTTTCAGTACAATCACCCTATATTCCTAA > REL606/3268734‑3268879 | ataAATATTCACAATAGGGCGCGAATATTACGCAAAACGCACGCCTTTGACAAGTGCTACAGTCAATACACgaaga > 1:694197/1‑76 (MQ=255)ataAATATTCACAATAGGGCGCGAATATTACGCAAAACGCACGCCTTTGACAAGTGCTACAGTCAATACACgaaga > 1:694215/1‑76 (MQ=255)ataAATATTCACAATAGGGCGCGAATATTACGCAAAACGCACGCCTTTGACAAGTGCTACAGTCAATACACgaaga > 1:1683145/1‑76 (MQ=255)ataAATATTCACAATAGGGCGCGAATATTACGCAAAACGCACGCCTTTGACAAGTGCTACAGTCAATACACgaaga > 2:1078052/1‑76 (MQ=255)ataAATATTCACAATAGGGCGCGAATATTACGCAAAACGCACGCCTTTGACAAGTGCTACAGTCAATACACgaaga > 1:1353807/1‑76 (MQ=255) taAATATTCACAATAGGGCGCGAATATTACGCAAAACGCACGCCTTTGACAAGTGCTACAGTCAATACACgaagaa > 1:908496/1‑76 (MQ=255) aaaTATTCACAATAGGGCGCGAATATTACGCAAAACGCGCGCCTTTGACAAGTGCTACAGTCAATACACGAAGaaa < 1:1958711/76‑1 (MQ=255) aaaTATTCACAATAGGGCGCGAATATTACGCAAAACGCACGCCTTTGACAAGTGCTACAGTCAATACACGAAGaaa > 1:761056/1‑76 (MQ=255) aaaTATTCACAATAGGGCGCGAATATTACGCAAAACGCACGCCTTTGACAAGTGCTACAGTCAATACACGAAGaaa < 1:1117945/76‑1 (MQ=255) aaTATTCACAATAGGGCGCGAATATTACGCAAAACGCACGCCTTTGACAAGTGCTACAGTCAATACACGAAGaaaa > 2:10550/1‑76 (MQ=255) aaTATTCACAATAGGGCGCGAATATTACGCAAAACGCACGCCTTTGACAAGTGCTACAGTCAATACACGAAGaaaa > 2:154912/1‑76 (MQ=255) atatTCACAATAGGGCGCGAATATTACGCAAAACGCACGCCTTTGACAAGTGCTACAGTCAATACACGAAGaaaaa < 1:1887522/76‑1 (MQ=255) atatTCACAATAGGGCGCGAATATTACGCAAAACGCACGCCTTTGACAAGTGCTACAGTCAATACACGAAGaaaaa < 2:1724253/76‑1 (MQ=255) tatTCACAATAGGGCGCGAATATTACGCAAAACGCACGCCTTTGACAAGTGCTACAGTCAATACACGAAGaaaaaa > 1:1471665/1‑76 (MQ=255) tatTCACAATAGGGCGCGAATATTACGCAAAACGCACGCCTTTGACAAGTGCTACAGTCAATACACGAAGaaaaaa > 1:1471673/1‑76 (MQ=255) tatTCACAATAGGGCGCGAATATTACGCAAAACGCACGCCTTTGACAAGTGCTACAGTCAATACACGAAGaaaaaa > 1:378434/1‑76 (MQ=255) ttCACAATAGGGCGCGAATATTACGCAAAACGCACGCCTTTGACAAGTGCTACAGTCAATACACGAAGaaaaaaac < 1:1234336/76‑2 (MQ=255) aaTAGGGCGCGAATATTACGCAAAACGCACGCCTTTAACAAGTGCTACAGTCAATACACGAAG‑AAAAAAACACAAc > 2:20369/1‑76 (MQ=255) tAGGGCGCGAATATTACGCAAAACGCACGCCTTTGACAAGTGCTACAGTCAATACACGAAGAAAAAAAACACAACt < 1:1282837/76‑1 (MQ=255) tAGGGCGCGAATATTACGCAAAACGCACGCCTTTGACAAGTGCTACAGTCAATACACGAAG‑AAAAAAACACAACtt < 2:273009/76‑1 (MQ=255) tAGGGCGCGAATATTACGCAAAACGCACGCCTTTGACAAGTGCTACAGTCAATACACGAAG‑AAAAAAACACAACtt < 1:719492/76‑1 (MQ=255) tAGGGCGCGAATATTACGCAAAACGCACGCCTTTGACAAGTGCTACAGTCAATACACGAAG‑AAAAAAACACAACtt > 1:1581320/1‑76 (MQ=255) ggCGCGAATATTACGCAAAACGCACGCCTTTGACAAGTGCTACAGTCAATACACGAAG‑AAAAAAACACAACTTGTa < 2:1825225/76‑1 (MQ=255) ggCGCGAATATTACGCAAAACGCACGCCTTTGACAAGTGCTACAGTCAATACACGAAG‑AAAAAAACACAACTTGTa < 2:1556314/76‑1 (MQ=255) gcgcGAATATTACGCAAAACGCACGCCTTTGACAAGTGCTACAGTCAATACACGAAG‑AAAAAAACACAACTTGTAc < 2:880659/76‑1 (MQ=255) gcacgAATATTACGCAAAACGCACGCCTTTGACAAGTGCTACAGTCAATACACGAAG‑AAAAAAACACAACTTGTAc < 2:458894/73‑1 (MQ=255) cgcgAATATTACGCAAAACGCACGCCTTTGACAAGTGCTACAGTCAATACACGAAG‑AAAAAAACACAACTTGTACg < 1:755040/76‑1 (MQ=255) gcgAATATTACGCAAAACGCACGCCTTTGACAAGTGCTACAGTCAATACACGAAGAAAAAAAACACAACTTGTACg > 1:690743/1‑76 (MQ=255) cgAATATTACGCAAAACGCACGCCTTTGACAAGTGCTACAGTCAATACACGAAG‑AAAAAAACACAACTTGTACGGt < 2:500260/76‑1 (MQ=255) cgAATATTACGCAAAACGCACGCCTTTGACAAGTGCTACAGTCAATACACGAAG‑AAAAAAACACAACTTGTACGGt < 2:1236737/76‑1 (MQ=255) atatTACGCAAAACGCACGCCTTTGACAAGTGCTACAGTCAATACACGAAG‑AAAGAAACACAACTTGTACGGTAAc < 2:1296808/76‑1 (MQ=255) atTACGCAAAACGCACGCCTTTGACAAGTGCTACAGTCAATACACGAAG‑AAAAAAACACAACTTGTACGGTAACGt > 1:994754/1‑76 (MQ=255) tACGCAAAACGCACGCCTTTGACAAGTGCTACAGTCAATACACGAAG‑AAAAAAACACAACTTGTACGGTAACGttt > 2:1626662/1‑76 (MQ=255) tACGCAAAACGCACGCCTTTGACAAGTGCTACAGTCAATACACGAAG‑AAAAAAACACAACTTGTACGGTAACGttt > 2:433090/1‑76 (MQ=255) tACGCAAAACGCACGCCTTTGACAAGTGCTACAGTCAATACACGAAG‑AAAAAAACACAACTTGTACGGTAACGttt < 2:840801/76‑1 (MQ=255) aCGCAAAACGCACGCCTTTGACAAGTGCTACAGTCAATACACGAAG‑AAAAAAACACAACTTGTACGGTAACGTTTa < 1:348651/76‑1 (MQ=255) aCGCAAAACGCACGCCTTTGACAAGTGCTACAGTCAATACACGAAG‑AAAAAAACACAACTTGTACGGTAACGTTTa < 1:1662808/76‑1 (MQ=255) cGCAAAACGCACGCCTTTGACAAGTGCTACAGTCAATACACGAAG‑AAAAAAACACAACTTGTACGGTAACGTTTAt > 1:1337478/1‑76 (MQ=255) cGCAAAACGCACGCCTTTGACAAGTGCTACAGTCAATACACGAAG‑AAAAAAACACAACTTGTACGGTAACGTTTAt < 2:949829/76‑1 (MQ=255) gCAAAACGCACGCCTTTGACAAGTGCTACAGTCAATACACGAAG‑AAAAAAACACAACTTGTACGGTAACGTTTATc < 1:816989/76‑1 (MQ=255) cAAAACGCACGCCTTTGACAAGTGCTACAGTCAATACACGAAG‑AAAAAAACACAACTTGTACGGTAACGTTTATCt < 2:1567615/76‑1 (MQ=255) cAAAACGCACGCCTTTGACAAGTGCTACAGTCAATACACGAAG‑AAAAAAACACAACTTGTACGGTAACGTTTATCt < 2:1583322/76‑1 (MQ=255) aaaaCGCACGCCTTTGACAAGTGCTACAGTCAATACACGAAG‑AAAAAAACACAACTTGTACGGTAACGTTTATCtg > 2:477609/1‑76 (MQ=255) aaaaCGCACGCCTTTGACAAGTGCTACAGTCAATACACGAAG‑AAAAAAACACAACTTGTACGGTAACGTTTATCtg < 1:1424650/76‑1 (MQ=255) aaaCGCACGCCTTTGACAAGTGCTACAGTCAATACACGAAG‑AAAAAAACACAACTTGTACGGTAACGTTTATCtgt > 2:1695102/1‑76 (MQ=255) aaCGCACGCCTTTGACAAGTGCTACAGTCAATACACGAAG‑AAAAAAACACAACTTGTACGGTAACGTTTATCtgtg < 2:1054775/76‑1 (MQ=255) aaCGCACGCCTTTGACAAGTGCTACAGTAAATACACGAAG‑AAAAAAACACAACTTGTACGGTAACGTTTATCtgtg < 1:576802/76‑1 (MQ=255) gcacgcCTTTGACAAGTGCTACAGTCAATACACGAAG‑AAAAAAACACAACTTGTACGGTAACGTTTATCTGTGCCa > 1:31767/1‑76 (MQ=255) cacgcCTTTGACAAGTGCTACAGTCAATACACGAAG‑AAAAAAACACAACTTGTACGGTAACGTTTATCTGTGCCAt > 2:999972/1‑76 (MQ=255) cacgcCTTTGACAAGTGCTACAGTCAATACACGAAG‑AAAAAAACACAACTTGTACGGTAACGTTTATCTGTGCCAt > 2:1395474/1‑76 (MQ=255) acgcCTTTGACAAGTGCTACAGTCAATACACGAAG‑AAAAAAACACAACTTGTACGGTAACGTTTATCTGTGCCAtt < 1:69987/76‑1 (MQ=255) acgcCTTTGACAAGTGCTACAGTCAATACACGAAG‑AAAAAAACACAACTTGTACGGTAACGTTTATCTGTGCCAtt < 2:1879698/76‑1 (MQ=255) acgcCTTTGACAAGTGCTACAGTCAATACACGAAG‑AAAAAAACACAACTTGTACGGTAACGTTTATCTGTGCCAtt < 1:1801847/76‑1 (MQ=255) gcCTTTGACAAGTGCTACAGTCAATACACGAAG‑AAAAAAACACAACTTGTACGGTAACGTTTATCTGTGCCAtttt > 1:157649/1‑76 (MQ=255) ccTTTGACAAGTGCTACAGTCAATACACGAAG‑AAAAAAACACAACTTGTACGGTAACGTTTATCTGTGCCAttttt < 1:148466/76‑1 (MQ=255) gACAAGTGCTACAGTCAATACACGAAG‑AAAAAAACACAACTTGTACGGTAACGTTTATCTGTGCCATTTTTTCAGt > 2:1734194/1‑76 (MQ=255) tGCTACAGTCAATACACGAAG‑AAAAAAACACAACTTGTACGGTAACGTTTATCTGTGCCATTTTTTCAGTACAATc > 2:256008/1‑76 (MQ=255) tGCTACAGTCAATACACGAAG‑AAAAAAACACAACTTGTACGGTAACGTTTATCTGTGCCATTTTTTCAGTACAATc < 1:1046885/76‑1 (MQ=255) cTACAGTCAATACACGAAG‑AAAAAAACACAACTTGTACGGTAACGTTTATCTGTGCCATTTTTTCAGTACAATCAc > 2:1719121/1‑76 (MQ=255) cTACAGTCAATACACGAAG‑AAAAAAACACAACTTGTACGGTAACGTTTATCTGTGCCATTTTTTCAGTACAATCAc > 1:1687960/1‑76 (MQ=255) cTACAGTCAATACACGAAG‑AAAAAAACACAACTTGTACGGTAACGTTTATCTGTGCCATTTTTTCAGTACAATCAc > 1:1687952/1‑76 (MQ=255) aCAGTCAATACACGAAGAAAAAAAACACAACTTGTACGGTAACGTTTATCTGTGCCATTTTTTCAGTACAATCAcc > 1:143350/1‑76 (MQ=255) aCAGTCAATACACGAAGAAAAAAAACACAACTTGTACGGTAACGTTTATCTGTGCCATTTTTTCAGTACAATCAcc > 2:871335/1‑76 (MQ=255) aGTCAATACACGAAG‑AAAAAAACACAACTTGTACGGTAACGTTTATCTGTGCCATTTTTTCAGTACAATCACCCta < 1:1826759/76‑1 (MQ=255) aGTCAATACACGAAG‑AAAAAAACACAACTTGTACGGTAACGTTTATCTGTGCCATTTTTTCAGTACAATCACCCta < 1:1467613/76‑1 (MQ=255) gTCAATACACGAAG‑AAAAAAACACAACTTGTACGGTAACGTTTATCTGTGCCATTTTTTCAGTACAATCACCCtat < 1:1209125/76‑1 (MQ=255) tCAATACACGAAGAAAAAAAACACAACTTGTACGGTAACGTTTATCTGTGCCATTTTTTCAGTACAATCACCCtat < 1:147393/76‑1 (MQ=255) cAATACACGAAG‑AAAAAAACACAACTTGTACGGTAACGTTTATCTGTGCCATTTTTTCAGTACAATCACCCTatat > 2:1785080/1‑76 (MQ=255) cAATACACGAAG‑AAAAAAACACAACTTGTACGGTAACGTTTATCTGTGCCATTTTTTCAGTACAATCACCCTatat < 2:1645084/76‑1 (MQ=255) cAATACACGAAG‑AAAAAAACACAACTTGTACGGTAACGTTTATCTGTGCCATTTTTTCAGTACAATCACCCTatat < 1:1065145/76‑1 (MQ=255) aTACACGAAG‑AAAAAAACACAACTTGTACGGTAACGTTTATCTGTGCCATTTTTTCAGTACAATCACCCTATATTc < 1:280704/76‑1 (MQ=255) acacGAAG‑AAAAAAACACAACTTGTACGGTAACGTTTATCTGTGCCATTTTTTCAGTACAATCACCCTATATTCCt < 1:1437875/76‑1 (MQ=255) cacGAAGAAAAAAAACACAACTTGTACGGTAACGTTTATCTGTGCCATTTTTTCAGTACAATCACCCTATATTCCt < 1:1283766/76‑1 (MQ=255) cacGAAG‑AAAAAAACACAACTTGTACGGTAACGTTTATCTGTGCCATTTTTTCAGTACAATCACCCTATATTCCTa < 2:586694/76‑1 (MQ=255) cacGAAG‑AAAAAAACACAACTTGTACGGTAACGTTTATCTGTGCCATTTTTTCAGTACAATCACCCTATATTCCTa < 1:213941/76‑1 (MQ=255) acGAAG‑AAAAAAACACAACTTGTACGGTAACGTTTATCTGTGCCATTTTTTCAGTACAATCACCCTATATTCCTaa < 1:1216426/76‑1 (MQ=255) | ATAAATATTCACAATAGGGCGCGAATATTACGCAAAACGCACGCCTTTGACAAGTGCTACAGTCAATACACGAAGAAAAAAAACACAACTTGTACGGTAACGTTTATCTGTGCCATTTTTTCAGTACAATCACCCTATATTCCTAA > REL606/3268734‑3268879 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |