| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 4,134,767 | (A)5→4 | intergenic (‑59/‑95) | argE ← / → argC | acetylornithine deacetylase/N‑acetyl‑gamma‑glutamyl‑phosphate reductase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 4,134,763 | 0 | A | . | 91.0% | 262.5 / 24.4 | 67 | intergenic (‑55/‑99) | argE/argC | acetylornithine deacetylase/N‑acetyl‑gamma‑glutamyl‑phosphate reductase |
| Reads supporting (aligned to +/- strand): ref base A (5/1); new base . (33/28); total (38/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.24e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.00e-01 | |||||||||||
GGCGGTAATTTGTTTTTCATTGTTGACACACCTCTGGTCATGATAGTATCAATATTCATGCAGTATTTATGAATAAAAATACACTAACGTTGAGCGTAATAAAACCCACCAGCCGTAAGGTGAATGTTTTACGTTTAACCTGGCA > REL606/4134689‑4134833 | ggCGGTAATTTGTTTTTCATTGTTGACACACCTCTGGTCATGATAGTATCAATATTCATGCAGTATTTATGAATaa < 2:1615256/76‑1 (MQ=255) gCGGTAATTTGTTTTTCATTGTTGACACACCTCTGGTCATGATAGTATCAATATTCATGCAGTATTTATGAATaaa > 2:827484/1‑76 (MQ=255) tAATTTGTTTTTCATTGTTGACACACCTCTGGTCATGATAGTATCAATATTCATGCAGTATTTATGAATaaaatct < 1:377920/76‑4 (MQ=255) tAATTTGTTTTTCATTGTTGACACACCTCTGGTCATGATAGTATCAATATTCATGCAGTATTTATGAATAAAAATa > 2:1991665/1‑76 (MQ=255) tttGTTTTTCATTGTTGACACACCTCTGGTCATGATAGTATCAATATTCATGCAGTATTTATGAAT‑AAAATctaac > 1:1438628‑M1/1‑71 (MQ=255) ttGTTTTTCATTGTTGACACACCTCTGGTCATGATAGTATCAATATTCATGCAGTATTTATGAAT‑AAAATctaacg < 1:1422292‑M1/76‑7 (MQ=255) tGTTTTTCATTGTTGACACACCTCTGGTCATGATAGTATCAATATTCATGCAGTATTTATGAAT‑AAAATctaacgt > 1:80172‑M1/1‑69 (MQ=255) tttttCATTGTTGACACACCTCTGGTCATGATAGTATCAATATTCATGCAGTATTTATGAATAAAAATACACTAAc > 1:1348900/1‑76 (MQ=255) tttttCATTGTTGACACACCTCTGGTCATGATAGTATCAATATTCATGCAGTATTTATGAAT‑AAAATctaacgttg < 1:617602‑M1/76‑10 (MQ=255) tttttCATTGTTGACACACCTCTGGTCATGATAGTATCAATATTCATGCAGTATTTATGAAT‑AAAATctaacgttg > 1:293631‑M1/1‑67 (MQ=255) tttttCATTGTTGACACACCTCTGGTCATGATAGTATCAATATTCATGCAGTATTTATGAAT‑AAAATctaacgttg < 1:1639394‑M1/76‑10 (MQ=255) ttttCATTGTTGACACACCTCTGGTCATGATAGTATCAATATTCATGCAGTATTTATGAAT‑AAAATctaacgttga < 1:1144604‑M1/76‑11 (MQ=255) tttCATTGTTGACACACCTCTGGTCATGATAGTATCAATATTCATGCAGTATTTATGAAT‑AAAATctaacgttgag < 1:748722‑M1/76‑12 (MQ=255) ttCATTGTTGACACACCTCTGGTCATGATAGTATCAATATTCATGCAGTATTTATGAAT‑AAAATctaacgttgagc > 2:1497434‑M1/1‑64 (MQ=255) cATTGTTGACACACCTCTGGTCATGATAGTATCAATATTCATGCAGTATTTATGAAT‑AAAATctaacgttgagcgt > 1:477330‑M1/1‑62 (MQ=255) cATTGTTGACACACCTCTGGTCATGATAGTATCAATATTCATGCAGTATTTATGAAT‑AAAATctaacgttgagcgt > 1:301569‑M1/1‑62 (MQ=255) cATTGTTGACACACCTCTGGTCATGATAGTATCAATATTCATGCAGTATTTATGAAT‑AAAATctaacgttgagcgt < 1:1766907‑M1/76‑15 (MQ=255) cATTGTTGACACACCTCTGGTCATGATAGTATCAATATTCATGCAGTATTTATGAAT‑AAAATctaacgttgagcgt > 1:275971‑M1/1‑62 (MQ=255) aTTGTTGACACACCTCTGGTCATGATAGTATCAATATTCATGCAGTATTTATGAAT‑AAAATctaacgttgagcgta > 1:1376599‑M1/1‑61 (MQ=255) ttgttgACACACCTCTGGTCATGATAGTATCAATATTCATGCAGTATTTATGAAT‑AAAATctaacgttgagcgtaa < 1:1390‑M1/76‑17 (MQ=255) ttgttgACACACCTCTGGTCATGATAGTATCAATATTCATGCAGTATTTATGAAT‑AAAATctaacgttgagcgtaa < 1:429465‑M1/76‑17 (MQ=255) ttgttgACACACCTCTGGTCATGATAGTATCAATATTCATGCAGTATTTATGAAT‑AAAATctaacgttgagcgtaa < 1:335639‑M1/76‑17 (MQ=255) ttgttgACACACCTCTGGTCATGATAGTATCAATATTCATGCAGTATTTATGAAT‑AAAATctaacgttgagcgtaa > 2:75044‑M1/1‑60 (MQ=255) gttgACACACCTCTGGTCATGATAGTATCAATATTCATGCAGTATTTATGAAT‑AAAATctaacgttgagcgtaata < 1:1101254‑M1/76‑19 (MQ=255) tgACACACCTCTGGTCATGATAGTATCAATATTCATGCAGTATTTATGAAT‑AAAATctaacgttgagcgtaataaa > 1:907928‑M1/1‑56 (MQ=255) gACACACCTCTGGTCATGATAGTATCAATATTCATGCAGTATTTATGAAT‑AAAATctaacgttgagcgtaataaaa > 1:643740‑M1/1‑55 (MQ=255) acacacCTCTGGTCATGATAGTATCAATATTCATGCAGTATTTATGAAT‑AAAATctaacgttgagcgtaataaaac > 2:815485‑M1/1‑54 (MQ=255) cacacCTCTGGTCATGATAGTATCAATATTCATGCAGTATTTATGAAT‑AAAATctaacgttgagcgtaataaaacc < 1:118760‑M1/76‑24 (MQ=255) acacCTCTGGTCATGATAGTATCAATATTCATGCAGTATTTATGAAT‑AAAATctaacgttgagcgtaataaaaccc > 1:1907101‑M1/1‑52 (MQ=255) acacCTCTGGTCATGATAGTATCAATATTCATGCAGTATTTATGAAT‑AAAATctaacgttgagcgtaataaaaccc > 1:1067767‑M1/1‑52 (MQ=255) ctctGGTCATGATAGTATCAATATTCATGCAGTATTTATGAAT‑AAAATctaacgttgagcgtaataaaacccacca < 1:1956965‑M1/76‑29 (MQ=255) tctGGTCATGATAGTATCAATATTCATGCAGTATTTATGAAT‑AAAATctaacgttgagcgtaataaaacccaccag < 2:623776‑M1/76‑30 (MQ=255) ctGGTCATGATAGTATCAATATTCATGCAGTATTTATGAAT‑AAAATctaacgttgagcgtaataaaacccaccagc > 1:250065‑M1/1‑46 (MQ=255) tGGTCATGATAGTATCAATATTCATGCAGTATTTATGAAT‑AAAATctaacgttgagcgtaataaaacccaccagcc < 1:1416440‑M1/76‑32 (MQ=255) gTCATGATAGTATCAATATTCATGCAGTATTTATGAAT‑AAAATctaacgttgagcgtaataaaacccaccagccgt > 2:320827‑M1/1‑43 (MQ=255) cATGATAGTATCAATATTCATGCAGTATTTATGAATAAAAATACACTAACGTTGAGCGTAATAAAACCCACCAGcc < 1:1198/76‑1 (MQ=255) cATGATAGTATCAATATTCATGCAGTATTTATGAAT‑AAAATctaacgttgagcgtaataaaacccaccagccgtaa > 1:388369‑M1/1‑41 (MQ=255) cATGATAGTATCAATATTCATGCAGTATTTATGAAT‑AAAATctaacgttgagcgtaataaaacccaccagccgtaa < 2:645072‑M1/76‑36 (MQ=255) tGATAGTATCAATATTCATGCAGTATTTATGAATAAAAATACACTAACGTTGAGCGTAATAAAACCCACCAGCCGt > 1:556200/1‑76 (MQ=255) aTAGTATCAATATTCATGCAGTATTTATGAAT‑AAAATctaacgttgagcgtaataaaacccaccagccgtaaggtg > 1:829617‑M1/1‑37 (MQ=255) tAGTATCAATATTCATGCAGTATTTATGAAT‑AAAATctaacgttgagcgtaataaaacccaccagccgtaaggtga > 1:788739‑M1/1‑36 (MQ=255) tAGTATCAATATTCATGCAGTATTTATGAAT‑AAAATctaacgttgagcgtaataaaacccaccagccgtaaggtga > 1:788743‑M1/1‑36 (MQ=255) aGTATCAATATTCATGCAGTATTTATGAAT‑AAAATctaacgttgagcgtaataaaacccaccagccgtaaggtgaa > 2:1199298‑M1/1‑35 (MQ=255) aTCAATATTCATGCAGTATTTATGAAT‑AAAATctaacgttgagcgtaataaaacccaccagccgtaaggtgaatgt > 2:1099917‑M1/1‑32 (MQ=255) aTCAATATTCATGCAGTATTTATGAAT‑AAAATctaacgttgagcgtaataaaacccaccagccgtaaggtgaatgt < 2:1302450‑M1/76‑45 (MQ=255) aTCAATATTCATGCAGTATTTATGAAT‑AAAATctaacgttgagcgtaataaaacccaccagccgtaaggtgaatgt > 1:1637385‑M1/1‑32 (MQ=255) aaTATTCATGCAGTATTTATGAAT‑AAAATctaacgttgagcgtaataaaccccaccagccgtaaggtgaatgtttt < 2:1035717‑M1/76‑48 (MQ=255) aaTATTCATGCAGTATTTATGAAT‑AAAATctaacgttgagcgtaataaaacccaccagccgtaaggtgaatgtttt < 2:1273686‑M1/76‑48 (MQ=255) aaTATTCATGCAGTATTTATGAAT‑AAAATctaacgttgagcgtaataaaacccaccagccgtaaggtgaatgtttt < 1:824720‑M1/76‑48 (MQ=255) aaTATTCATGCAGTATTTATGAAT‑AAAATctaacgttgagcgtaataaaacccaccagccgtaaggtgaatgtttt > 2:1803159‑M1/1‑29 (MQ=255) atatTCATTCAGTATTTATGAAT‑AAAATctaacgttgagcgtaataaaccccaccagccgtaaggtgaatgtttta < 2:114622‑M1/76‑49 (MQ=255) atatTCATGCAGTATTTATGAAT‑AAAATctaacgttgagcgtaataaaaccccccagccgtaaggtgaatgtttta < 1:1355883‑M1/76‑49 (MQ=255) atatTCATGCAGTATTTATGAAT‑AAAATctaacgttgagcgtaataaaacccaccagccgtaaggtgaatgtttta < 2:1485962‑M1/76‑49 (MQ=255) tatTCATGCAGTATTTATGAAT‑AAAATctaacgttgagcgtaataaaacccaccagccgtaaggtgaatgttttac > 1:940424‑M1/1‑27 (MQ=255) tatTCATGCAGTATTTATGAAT‑AAAATctaacgttgagcgtaataaaacccaccagccgtaaggtgaatgttttac > 2:32124‑M1/1‑27 (MQ=255) tatTCATGCAGTATTTATGAAT‑AAAATctaacgttgagcgtaataaaacccaccagccgtaaggtgaatgttttac > 1:16479‑M1/1‑27 (MQ=255) atTCATGCAGTATTTATGAATAAAAATACACTAACGTTGAGCGTAATAAAACCCACCAGCCGTAAGGTGAATGttt > 1:1418342/1‑76 (MQ=255) tCATGCAGTATTTATGAAT‑AAAATctaacgttgagcgtaataaaccccaccagccgtaaggtgaatgttttacgtt < 1:1497434‑M1/76‑53 (MQ=255) tCATGCAGTATTTATGAAT‑AAAATctaacgttgagcgtaataaaccccaccagccgtaaggtgaatgttttacgtt < 2:434812‑M1/76‑53 (MQ=255) tCATGCAGTATTTATGAAT‑AAAATctaacgttgagcgtaataaaacccaccagccgtaaggtgaatgttttacgtt > 2:1684534‑M1/1‑24 (MQ=255) aTGCAGTATTTATGAAT‑AAAATctaacgttgagcgtaataaaacccaccagccgtaaggtgaatgttttacgttta > 1:1420459‑M1/1‑22 (MQ=255) aTGCAGTATTTATGAAT‑AAAATctaacgttgagcgtaataaaacccaccagccgtaaggtgaatgttttacgttta > 1:1420442‑M1/1‑22 (MQ=255) aTGCAGTATTTATGAAT‑AAAATctaacgttgagcgtaataaaacccaccagccgtaaggtgaatgttttacgctta > 1:1420456‑M1/1‑22 (MQ=255) gCAGTATTTATGAAT‑AAAATctaacgttgagcgtaataaaacccaccagccgtaaggtgaatgttttacgtttaac < 2:173629‑M1/76‑57 (MQ=255) aGTATTTATGAAT‑AAAATctaacgttgagcgtaataaacccccccagccgtaaggtgaatgttttacgtttaacct < 2:491004‑M1/76‑59 (MQ=255) tttATGAAT‑AAAATctaacgttgagcgtaataaaacccaccagccgtaaggtgaatgttttacgtttaacctggca > 1:3642‑M1/1‑14 (MQ=255) ttATGAATAAAAATACACTAACGTTGAGCGTAATAAAACCCACCAGCCGTAAGGTGAATGTTTTACGTTTAACCTg > 1:1748125/1‑76 (MQ=255) ttATGAAT‑AAAATctaacgttgagcgtaataaaccccaccagccgtaaggtgaatgttttacgtttaacctggcaa < 2:618444‑M1/76‑64 (MQ=255) ttATGAAT‑AAAATctaacgttgagcgtaataaaacccaccagccgtaaggtgaatgttttacgtttaacctggcaa > 1:1478063‑M1/1‑13 (MQ=255) tATGAAT‑AAAATctaacgttgagcgtaataaaacccaccagccgtaaggtgaatgttttacgtttaacctggcaac < 2:1818221‑M1/76‑65 (MQ=255) aTGAAT‑AAAATctaacgttgagcgtaaaaaaccccaccagccgtaaggtgaatgttttacgtttaacctggcaacc < 2:424763‑M1/76‑66 (MQ=255) | GGCGGTAATTTGTTTTTCATTGTTGACACACCTCTGGTCATGATAGTATCAATATTCATGCAGTATTTATGAATAAAAATACACTAACGTTGAGCGTAATAAAACCCACCAGCCGTAAGGTGAATGTTTTACGTTTAACCTGGCA > REL606/4134689‑4134833 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |