Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	1,305,682	(A)6→5	coding (558/1461 nt)	cls ←	cardiolipin synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	1,305,677	0	A	.	100.0%	161.4 / NA	34	coding (563/1461 nt)	cls	cardiolipin synthetase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (17/17);  total (17/17)

TCAGATTGACCTTTAAGGCTTCGACCACTTCAATACCGGCATTACGCATTAGCTCGGGCCACGGGCTGCGGAAAAAAGCCACACTCCCGGCGGAGTCGAGCATCAATCGGCAATGAATGCCGCGTCGCGCAGCGGCCATTAAT  >  REL606/1305606‑1305748                                                                        |                                                                        tCAGATTGACCTTTAAGGCTTCGACCACTTCAATACCGGCATTACGCATTAGCTCGGGCCACGGGCTGCGGaaaaa                                                                     >  2:1304588/1‑76 (MQ=255)     aTTGACCTTTAAGGCTTCGACCACTTCAATACCGGCATTACGCATCAGCTCGGGCCACGGGCGGCGGAAAAAAGcc                                                                 >  2:1436405/1‑76 (MQ=255)       tGACCTTTAAGGCTTCGACCACTTCAATACCGGCATTACGCATTAGCTCGGGCCACGGGCTGCGG‑AAAAAGCcaca                                                              >  2:100501/1‑76 (MQ=255)         gccTTTAAGGCTTCGACCACTTCAATACCGGCATTACGCATTAGCTCGGGCCACGGGCTGCGG‑AAAAAGCCACACt                                                            <  1:1604341/75‑1 (MQ=255)          ccTTTAAGGCTTCGACCACTTCAATACCGGCATTACGCATTAGCTCGGGCCACGGGCTGCGG‑AAAAAGCCACACTc                                                           <  2:795637/76‑1 (MQ=255)              tAAGGCTTCGACCACTTCAATACCGGCATTACGCATTAGCTCGGGCCACGGGCTGCGG‑AAAAAGCCACACTCCcgg                                                       >  1:890920/1‑76 (MQ=255)              tAAGGCTTCGACCACTTCAATACCGGCATTACGCATTAGCTCGGGCCACGGGCTGCGG‑AAAAAGCCACACTCCcgg                                                       <  1:68812/76‑1 (MQ=255)              tAAGGCTTCGACCACTTCAATACCGGCATTACGCATTAGCTCGGGCCACGGGCTGCGG‑AAAAAGCCACACTCCcgg                                                       >  1:752158/1‑76 (MQ=255)                aGGCTTCGACCACTTCAATACCGGCATTACGCATTAGCTCGGGCCACGGGCTGCGG‑AAAAAGCCACACTCCcggcg                                                     >  2:1893004/1‑76 (MQ=255)                 ggCTTCGACCACTTCAATACCGGCATTACGCATTAGCTCGGGCCACGGGCTGCGG‑AAAAAGCCACACTCCcggcgg                                                    <  2:1943982/76‑1 (MQ=255)                 ggCTTCGACCACTTCAATACCGGCATTACGCATTAGCTCGGGCCACGGGCTGCGG‑AAAAAGCCACACTCCcggcgg                                                    >  1:756233/1‑76 (MQ=255)                      cGACCACTTCAATACCGGCATTACGCATTAGCTCGGGCCACGGGCTGCGG‑AAAAAGCCACACTCCCGGCGGAGTCg                                               <  2:1809035/76‑1 (MQ=255)                        aCCACTTCAATACCGGCATTACGCATTAGCTCGGGCCACGGGCTGCGG‑AAAAAGCCACACTCCCGGCGGAGTCGAg                                             <  1:969385/76‑1 (MQ=255)                           aCTTCAATACCGGCATTACGCATTAGCTCGGGCCACGGGCTGCGG‑AAAAAGCCACACTCCCGGCGGAGTCGAGCAt                                          <  1:1692957/76‑1 (MQ=255)                              tCAATACCGGCATTACGCATTAGCTCGGGCCACGGGCTGCGG‑AAAAAGCCACACTCCCGGCGGAGTCGAGCATCaa                                       >  1:1244926/1‑76 (MQ=255)                               cAATACCGGCATTACGCATTAGCTCGGGCCACGGGCTGCGG‑AAAAAGCCACACTCCCGGCGGAGTCGAGCATCAAt                                      >  1:505020/1‑76 (MQ=255)                                  tACCGGCATTACGCATTAGCTCGGGCCACGGGCTGCGG‑AAAAAGCCACACTCCCGGCGGAGTCGAGCATCAATCgg                                   <  1:381370/76‑1 (MQ=255)                                  tACCGGCATTACGCATTAGCTCGGGCCACGGGCTGCGG‑AAAAAGCCACACTCCCGGCGGAGTCGAGCATCAATCgg                                   <  1:1355517/76‑1 (MQ=255)                                  tACCGGCATTACGCATTAGCTCGGGCCACGGGCTGCGG‑AAAAAGCCACACTCCCGGCGGAGTCGAGCATCAATCgg                                   <  1:1321813/76‑1 (MQ=255)                                   aCCGGCATTACGCATTAGCTCGGGCCACGGGCTGCGG‑AAAAAGCCACACTCCCGGCGGAGTCGAGCATCAATCGGc                                  <  1:982734/76‑1 (MQ=255)                                     cGGCATTACGCATTAGCTCGGGCCACGGGCTGCGG‑AAAAAGCCACACTCCCGGCGGAGTCGAGCATCAATCGGCaa                                >  2:513223/1‑76 (MQ=255)                                        cATTACGCATTAGCTCGGGCCACGGGCTGCGG‑AAAAAGCCACACTCCCGGCGGAGTCGAGCATCAATCGGCaatga                             >  2:15285/1‑76 (MQ=255)                                          ttACGCATTAGCTCGGGCCACGGGCTGCGG‑AAAAAGCCACACTCCCGGCGGAGTCGAGCATCAATCGGCaatgaat                           >  2:1380899/1‑76 (MQ=255)                                                   aGCTCGGGCCACGGGCTGCGG‑AAAAAGCCACACTCCCGGCGGAGTCGAGCATCAATCGGCAATGAATGCCGCGTcg                  <  2:1363208/76‑1 (MQ=255)                                                    gCTCGGGCCCCGGGCTGCGG‑AAAAAGCCACACTCCCGGCGGAGTCGAGCATCAATCGGCAATGAATGCCGCGTcgc                 <  2:1021044/76‑1 (MQ=255)                                                     cTCGGGCCACGGGCTGCGG‑AAAAAGCCACACTCCCGGCGGAGTCGAGCATCAATCGGCAATGAATGCCGCGTcgcg                >  1:466732/1‑76 (MQ=255)                                                       cGGGCCCCGGGCTGCCG‑AAAAAGCCACACTCCCGGCGGAGTCGAGCATCAATCGGCAATGAATGCCGCGTCGCGCa              <  2:1977487/76‑1 (MQ=255)                                                       cGGGCCACGGGCTGCGG‑AAAAAGCCACACTCCCGGCGGAGTCGAGCCTCAATCGGCAATGAAAGCCGCGTCGCGCa              >  2:953662/1‑76 (MQ=255)                                                       cGGGCCACGGGCTGCGG‑AAAAAGCCACACTCCCGGCGGAGTCGAGCATCAATCGGCAATGAATGCCGCGTCGCGCa              <  2:666798/76‑1 (MQ=255)                                                       cGGGCCACGGGCTGCGG‑AAAAAGCCACACTCCCGGCGGAGTCGAGCATCAATCGGCAATGAATGCCGCGTCGCGCa              <  2:887444/76‑1 (MQ=255)                                                        gggCCACGGGCTGCGG‑AAAAAGCCACACTCCCGGCGGAGTCGAGCATCAATCGGCAATGAATGCCGCGTCGCGCAg             >  1:1451969/1‑76 (MQ=255)                                                         ggCCACGGGCTGCGG‑AAAAAGCCACACTCCCGGCGGAGTCGAGCATCAATCGGCAATGAATGCCGCGTCGCGCAGc            >  2:1459874/1‑76 (MQ=255)                                                          gCCACGGGCTGCGG‑AAAAAGCCACACTCCCGGCGGAGTCGAGCATCAATCGGCAATGAACACCGCGTCGCGCACCg           >  2:391633/1‑76 (MQ=255)                                                           ccACGGGCTGCGG‑AAAAAGCCACACTCCCGGCGGAGTCGAGCATCAATCGGCAATGAATGCCGCGTCGCGCAGCgg          >  2:1036912/1‑76 (MQ=255)                                                            cACGGGCTGCGG‑AAAAAGCCACACTCCCGGCGGAGTCGAGCATCAATCGGCAATGAATGCCGCGTCGCGCAGCGGc         >  2:1158596/1‑76 (MQ=255)                                                              cGGGCTGCGG‑AAAAAGCCACACTCCCGGCGGAGTCGAGCATCAATCGGCAATGAATGCCGCGTCGCGCAGCGGCCa       <  2:1638275/76‑1 (MQ=255)                                                                   tGCGG‑AAAAAGCCCCACGCCCGGCGGAGTCGAGCATCAATCGGCAATGAATGCCGCGTCGCGCAGCGGCCATTAAt  <  1:875031/76‑1 (MQ=255)                                                                        |                                                                        TCAGATTGACCTTTAAGGCTTCGACCACTTCAATACCGGCATTACGCATTAGCTCGGGCCACGGGCTGCGGAAAAAAGCCACACTCCCGGCGGAGTCGAGCATCAATCGGCAATGAATGCCGCGTCGCGCAGCGGCCATTAAT  >  REL606/1305606‑1305748

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑