Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	REL606	4,524,522	IS186 (+) +6 bp	coding (494‑499/549 nt)	fimA →	major type 1 subunit fimbrin (pilin)

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	REL606	2772854 =	NA (NA)	74 (1.240)	49/144	0.1	100%	noncoding (1/1343 nt)	IS186	repeat region
	?	REL606	= 4524527	0 (0.000)					coding (499/549 nt)	fimA	major type 1 subunit fimbrin (pilin)
*	?	REL606	= 2774196	NA (NA)	61 (1.020)	46/144	0.2	100%	noncoding (1343/1343 nt)	IS186	repeat region
	?	REL606	4524522 =	0 (0.000)					coding (494/549 nt)	fimA	major type 1 subunit fimbrin (pilin)

CGCGAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  REL606/2774120‑2774196 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑gggCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAGGTTCAGGGAC  >  REL606/4524522‑4524596                                                                                                                                                          CGCGAGGCATCGTCTTAACGAGGCACCGAGGGGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCGTATGG                                                                              >  1:789291/1‑76 CGCGAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGG                                                                              >  1:789304/1‑76  GCGAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGG                                                                             <  1:901434/76‑1  GCGAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGG                                                                             <  2:807859/76‑1  GCGAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGG                                                                             <  2:1311163/76‑1  GCGAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGG                                                                             >  1:592139/1‑76  GCGAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGG                                                                             >  1:429587/1‑76  GCGAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGG                                                                             <  1:357565/76‑1  GCGAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGG                                                                             <  1:357551/76‑1  GCGAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGG                                                                             <  2:1832806/76‑1     AGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGCCG                                                                          >  1:788447/1‑76     AGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGCCG                                                                          >  1:1776526/1‑76       GCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGCCGCA                                                                        <  1:1883393/76‑1           ctTCTTAACGAGGCACCGACGCGTCGCATTCATCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGCCGCAACCC                                                                    <  2:1325984/74‑1            GTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGCCGCAACCCC                                                                   <  2:877977/76‑1            GTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGCCGCAACCCC                                                                   <  1:1857463/76‑1              CTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGCCGCAACCCCGG                                                                 <  1:472135/76‑1                TAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGCCGCAACCCCGGGT                                                               <  1:514568/76‑1                TAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGCCGCAACCCCGGGT                                                               <  1:330252/76‑1                  ACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGCCGCAACCCCGGGTGC                                                             <  1:375771/76‑1                    GAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGCCGCAACCCCGGGTGCTG                                                           <  2:1441775/76‑1                      GGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGCCGCAACCCCGGGTGCTGCT                                                         >  1:511600/1‑76                       GCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGCCGCAACCCCGGGTGCTGCTA                                                        <  2:275994/76‑1                       GCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGCCGCAACCCCGGGTGCTGCTA                                                        <  2:1588859/76‑1                         ACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGCCGCAACCCCGGGTGCTGCTAAT                                                      <  1:387025/76‑1                          CCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGCCGCAACCCCGGGTGCTGCTAATG                                                     <  1:1556784/76‑1                           CGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGCCGCAACCCCGGGTGCTGCTAATGC                                                    >  2:1528054/1‑76                           CGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGCCGCAACCCCGGGTGCTGCTAATGC                                                    <  2:435894/76‑1                            GAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGCCGCAACCCCGGGTGCTGCTAATGCG                                                   >  2:243234/1‑76                             AGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGCCGCAACCCCGGGTGCTGCTAATGCGG                                                  <  1:199165/76‑1                             AGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGCCGCAACCCCGGGTGCTGCTAATGCGG                                                  >  2:1670126/1‑76                              GGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGCCGCAACCCCGGGTGCTGCTAATGCGGA                                                 >  2:437854/1‑76                               GCGTCGCATTCTGCAGATGGTTCAACCCTTAAGTGAGCGCTTATGGGCCGCAACCCCGGGTGCTGCTAATGCGGAT                                                <  2:257767/76‑1                                CGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGCCGCAACCCCGGGTGCTGCTAATGCGGATG                                               <  2:783258/76‑1                                   CGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGA                                            >  1:589080/1‑76                                   CGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGA                                            >  2:1304285/1‑76                                    GCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGAC                                           >  1:254829/1‑76                                    GCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGAC                                           <  2:793748/76‑1                                     CATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACC                                          >  2:1995341/1‑76                                       TTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTT                                        <  1:1242664/76‑1                                       TTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTT                                        <  2:1706220/76‑1                                       TTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTT                                        <  2:1826355/76‑1                                          TTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAA                                     >  2:1253182/1‑76                                           TCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAG                                    >  1:1060884/1‑76                                           TCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAG                                    <  1:1692196/76‑1                                           TCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAG                                    <  1:1796674/76‑1                                            CAGATGGTTCAACCCTTAAGTTAGCGCTTATGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGG                                   >  2:1234210/1‑76                                              GATGGTTCAACCCTTAAGTTAGCGCTTATGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTT                                 <  2:24269/76‑1                                               ATGGTTCAACCCTTAAGTTAGCGCTTATGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTC                                >  1:889006/1‑76                                               ATGGTTCAACCCTTAAGTTAGCGCTTATGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTC                                <  1:183775/76‑1                                                TGGTTCAACCCTTAAGTTAGCGCTTATGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCA                               <  2:474805/76‑1                                                  GTTCAACCCTTAAGTTAGCGCTTATGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGT                             >  2:793184/1‑76                                                      AACCCTTAAGTTAGCGCTTATGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCA                         <  1:1973228/76‑1                                                         CCTTAAGTTAGCGCTTATGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGGTCAGTATCAATA                      >  1:773952/1‑76                                                          CTTAAGTTAGCGCTTATGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAA                     <  2:901048/76‑1                                                          CTTAAGTTAGCGCTTATGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAA                     <  1:786510/76‑1                                                           TTAAGTTAGCGCTTATGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAAC                    >  1:1944756/1‑76                                                           TTAAGTTAGCGCTTATGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAAC                    >  1:366265/1‑76                                                           TTAAGTTAGCGCTTATGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAAC                    >  1:1218627/1‑76                                                             AAGTTAGCGCTTATGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCT                  <  1:1275234/76‑1                                                               GTTAGCGCTTATGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTAC                >  2:863317/1‑76                                                                 TAGCGCTTATGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCC              <  1:793615/76‑1                                                                 TAGCGCTTATGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCC              <  1:529730/76‑1                                                                  AGCGCTTATGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCA             >  1:1984109/1‑76                                                                  AGCGCTTATGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCA             >  1:1413616/1‑76                                                                     GCTTATGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAGGT          >  2:1805587/1‑76                                                                      CTTATGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAGGTT         >  1:1827299/1‑76                                                                      CTTATGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAGGTT         >  2:477594/1‑76                                                                       TTATGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAGGTTC        <  2:1603868/76‑1                                                                       TTATGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAGGTTC        <  1:733892/76‑1                                                                        TATGGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAGGTTCA       <  2:1560088/76‑1                                                                           GGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAGGTTCAGGG    <  1:1497493/76‑1                                                                           GGGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAGGTTCAGGG    <  1:1168934/76‑1                                                                            GGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAGGTTCAGGGA   <  1:1765921/76‑1                                                                            GGCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAGGTTCAGGGA   <  1:1027722/76‑1                                                                             GCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAGGTTCAGGGAC  <  2:1322965/76‑1                                                                                                                                                          CGCGAGGCATCGTCTTAACGAGGCACCGAGGCGTCGCATTCTTCAGATGGTTCAACCCTTAAGTTAGCGCTTATGGG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  REL606/2774120‑2774196 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑gggCCGCAACCCCGGGTGCTGCTAATGCGGATGCGACCTTCAAGGTTCAGTATCAATAACCTACCCAGGTTCAGGGAC  >  REL606/4524522‑4524596

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.