Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*REL6064,000,2950TG40.4% 63.5 / 19.6 48G533G (GGT→GGGubiBputative ubiquinone biosynthesis protein UbiB
Reads supporting (aligned to +/- strand):  ref base T (9/19);  new base G (19/0);  total (29/19)
Fisher's exact test for biased strand distribution p-value = 1.46e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

TTAGTATTAAGTGGCACATTCTTGTTGGTCAGCCGACCTGAATGGGGGCTGATGCCCGGCTGGTTAATGGCAGGTGGTCTGATCGCCTGGTTTGTCGGTTGGCGCAAAACACGCTGATTTTTTCATCGCTCAAGGCGGGCCGTGTAAC  >  REL606/4000221‑4000368
                                                                          |                                                                         
ttAGTATTAAGTGGCACATTCTGGTTGGTCAGCCGACCTGAATGGGGGCTGATGCCCGGCTGGTTAATGGCAggtg                                                                          <  2:318021/76‑1 (MQ=255)
 tAGTATTAAGTGGCACATTCTTGTTGGTCAGCCGACCTGAATGGGGGCTGATGCCCGGCTGGTTAATGGCAggggg                                                                         >  2:56304/1‑76 (MQ=255)
  aGTATTAAGTGGCACATTCTTGTTGGTCAGCCGACCTGAATGGGGGCTGATGCCCGGCTGGTTAATGGCAggtggt                                                                        <  1:1496394/76‑1 (MQ=255)
  aGTATTAAGTGGCACATTCTTGTTGGTCAGCCGACCTGAATGGGGGCTGATGCCCGGCTGGTTAATGGCAggtggt                                                                        <  1:62437/76‑1 (MQ=255)
  aGTATTAAGTGGCACATTCTTGTTGGTCAGCCGACCTGAATGGGGGCTGATGCCCGGCTGGTTAATGGCAgggggt                                                                        >  1:1677465/1‑76 (MQ=255)
  aGTATTAAGTGGCACATTCTTGTTGGTCAGCCGACCTGAATGGGGGCTGATGCCCGGCTGGTTAATGGCAgggggg                                                                        >  1:377888/1‑75 (MQ=255)
  aGTATTAAGTGGCACATTCTTGTTGGTCAGCCGACCTGAATGGGGGCTGATGCCCGGCTGGTTAATGGCAgggggg                                                                        >  1:1436455/1‑75 (MQ=255)
  aGTATTAAGTGGCACATTCTTGTTGGTCAGCCGACCTGAATGGGGGCTGATGCCCGGCTGGTTAATGGCAgggggg                                                                        >  1:1337477/1‑75 (MQ=255)
    tATTAAGTGGCACATTCTTGTTGGTCAGCCGACCTGAATGGGGGCTGATGCCCGGCTGGTTAATGGCAGGTGGTCt                                                                      <  1:699560/76‑1 (MQ=255)
       tAAGTGGCACATTCTTGTTGGTCAGCCGACCTGAATGGGGGCTGATGCCCGGCTGGTTAATGGCAGGTGGTCTGag                                                                   >  1:633479/1‑75 (MQ=255)
       tAAGTGGCACATTCTTGTTGGTCAGCCGACCTGAATGGGGGCTGATGCCCGGCTGGTTAATGGCAGGGGGTCTGAt                                                                   >  1:1424745/1‑76 (MQ=255)
       tAAGTGGCACATTCTTGTTGGTCAGCCGACCTGAATGGGGGCTGATGCCCGGCTGGTTAATGGCAGGGGGTCTGAt                                                                   >  2:783831/1‑76 (MQ=255)
       tAAGTGGCACATTCTTGTTGGTCAGCCGACCTGAATGGGGGCTGATGCCCGGCTGGTTAATGGCAGGGGGGCTGAt                                                                   >  2:783846/1‑76 (MQ=255)
        aaGTGGCACATTCTTGTTGGTCAGCCGACCTGAATGGGGGCTGATGCCCGGCTGGTTAATGGCAGGGGGGCTGGTc                                                                  >  2:1348415/1‑76 (MQ=255)
            ggCACATTCTTGTTGGTCAGCCGACCTGAATGGGGGCTGATGCCCGGCTGGTTAATGGCAGGTGGTCTGATCGCCt                                                              <  2:1203975/76‑1 (MQ=255)
             gCACATTCTTGTTGGTCAGCCGACCTGAATGGGGGCTGATGCCCGGCTGGTTAATGGCAGGGGGTCTGATCGCCTg                                                             >  1:1286575/1‑76 (MQ=255)
              cacaTTCTTGTTGGTCAGCCGACCTGAATGGGGGCTGATGCCCGGCTGGTTAATGGCAGGGGGGCTGATCGCCTgg                                                            >  2:1640115/1‑76 (MQ=255)
              cacaTTCTTGTTGGTCAGCCGACCTGAATGGGGGCTGATGCCCGGCTGGTTAATGGCAGGCGGGCTGATTCCCTgg                                                            >  1:443869/1‑76 (MQ=255)
                caTTCTTGTTGGTCAGCCGACCTGAATGGGGGCTGATGCCCGGCTGGTTAATGGCAGGTGGTCTGATCGCCTGGtt                                                          <  2:1377983/76‑1 (MQ=255)
                caTTCTTGTTGGTCAGCCGACCTGAATGGGGGCTGATGCCCGGCTGGTTAATGGCAGGGGGTCTGATCGCCTGGtt                                                          >  1:1647795/1‑76 (MQ=255)
                 aTTCTTGTTGGTCAGCCGACCTGAATGGGGGCTGATGCCCGGCTGGTTAATGGCAGGTGGGCTGATCGCCTGGttt                                                         >  2:970185/1‑76 (MQ=255)
                 aTTCTTGTTGGTCAGCCGACCTGAATGGGGGCTGATGCCCGGCTGGTTAATGGCAGGGGGGCTGATCGCCTGGttt                                                         >  2:647708/1‑76 (MQ=255)
                  ttCTTGTTGGTCAGCCGACCTGAATGGGGGCTGATGCCCGGCTGGTTAATGGCAGGTGGTCTGATCGCCTGGTTTg                                                        <  2:1187390/76‑1 (MQ=255)
                  ttCTTGTTGGTCAGCCGACCTGAATGGGGGCTGATGCCCGGCTGGTTAATGGCAGGGGGTCTGATCGCCTGGTTTg                                                        >  1:17904/1‑76 (MQ=255)
                     ttgttgGTCAGCCGACCTGAATGGGGGCTGATGCCCGGCTGGTTAATGGCAGGTGGTCTGATCGCCTGGTTTGTCg                                                     <  1:1174729/76‑1 (MQ=255)
                     ttgttgGTCAGCCGACCTGAATGGGGGCTGATGCCCGGCTGGTTAATGGCAGGGGGGCTGATCGCCTGGTTTGTCg                                                     >  2:922226/1‑76 (MQ=255)
                      tgttgGTCAGCCGACCTGAATGGGGGCTGATGGCCGGCTGGTTAATGGCAGGTGGTCTGATCGCCTGGTTTGTCgg                                                    <  2:872251/76‑1 (MQ=255)
                      tgttgGTCAGCCGACCTGAATGGGGGCTGATGCCCGGCTGGTTAATGGCAGGTGGTCTGATCGCCTGGTTTGTCgg                                                    <  1:135141/76‑1 (MQ=255)
                      tgttgGTCAGCCGACCTGAATGGGGGCTGATGCCCGGCTGGTTAATGGCAGGGGGTCTGATCGCCTGGGTTGTCgg                                                    >  2:1740586/1‑76 (MQ=255)
                       gttgGTCAGCCGACCTGAATGGGGGCTGATGCCCGGCTGGTTAATGGCAGGGGGGCTGATCGCCTGGTTTGTCGGt                                                   >  1:1663636/1‑76 (MQ=255)
                        ttgGTCAGCCGACCTGAATGGGGGCTGATGCCCGGCTGGTTAATGGCAGGTGGTCTGATCGCCTGGTTTGTCGGtt                                                  <  2:954597/76‑1 (MQ=255)
                        ttgGTCAGCCGACCTGAATGGGGGCTGATGCCCGGCTGGTTAATGGCAGGTGGTCTGATCGCCTGGTTTGTCGGtt                                                  <  1:314243/76‑1 (MQ=255)
                         tgGTCAGCCGACCTGAATGGGGGCTGATGCCCGGCTGGTTAATGGCAGGTGGTCTGATCGCCTGGTTTGTCGGTTg                                                 <  1:1043477/76‑1 (MQ=255)
                         tgGTCAGCCGACCTGAATGGGGGCTGATGCCCGGCTGGTTAATGGCAGGTGGTCTGATCGCCTGGTTTGTCGGTTg                                                 <  2:1843528/76‑1 (MQ=255)
                          ggTCAGCCGACCTGAATGGGGGCTGATGCCCGGCTGGTTAATGGCAGGGGGTCTGATCGCCTGGTTTGTCGGTTgg                                                >  1:1469053/1‑76 (MQ=255)
                          ggTCAGCCGACCTGAATGGGGGCTGATGCCCGGCTGGTTAATGGCAGGAGGGCTGAGCGCCTTGTTTGTCGGTTgg                                                >  1:1586419/1‑76 (MQ=255)
                           gTCAGCCGACCTGAATGGGGGCTGATGCCCGGCTGGTTAATGGCAGGTGGTCTGATCGCCTGGTTTGTCGGTTGgc                                               >  2:1062568/1‑76 (MQ=255)
                                       gAATGGGGGCTGATGCCCGGCTGGTTAATGGCAGGTGGTCTGATCGCCTGGGTTGTCGGTTGGCGCAAAACAAGCt                                   >  1:933025/1‑76 (MQ=255)
                                       gAATGGGGGCTGATGCCCGGCTGGTTAATGGCAGGGGGTCTGATCGCCTGGTTTGTCGGTTGGCGCAAAACACGCt                                   >  1:1698584/1‑76 (MQ=255)
                                        aaTGGGGGCTGATGCCCGGCTGGTTAATGGCAGGGGGTCTGATCGCCTGGTTTGTCGGTTGGCGGAAAACACGCTg                                  >  2:1223161/1‑76 (MQ=255)
                                          tGGGGGCTGATGCCCGGCTGGTTAATGGCAGGGGGTCTGATCGCCTGGTTTGTCGGTTGGCGCAAAACACGCTGAt                                >  2:1134509/1‑76 (MQ=255)
                                           gggggCTGATGCCCGGCTGGTTAATGGCAGGTGGTCTGATCGCCTGGTTTGTCGGTTGGCGCAAAACACGCTGAtt                               <  2:1721099/76‑1 (MQ=255)
                                           gggggCTGATGCCCGGCTGGTTAATGGCAGGGGGGCTGATCGCCCGGGTTGTGGGGTGGGGCAAAAAACGCGGAtt                               >  1:658816/1‑76 (MQ=255)
                                                 tGATGCCCGGCTGGTTAATGGCAGGTGGTCTGATCGCCTGGTTTGTCGGTTGGCGCAAAACACGCTGATTTTTTCa                         >  1:301937/1‑76 (MQ=255)
                                                 tGATGCCCGGCTGGTTAATGGCAGGTGGTCTGATCGCCTGGTTTGTCGGTTGGCGCAAAACACGCTGATTTTTTCa                         >  1:1437391/1‑76 (MQ=255)
                                                        cGGCTGGTTAATGGCAGGGGGGCTGATCGCCTGGTTTGTCGGTTGGCGCAAAAAACGCTGATTTTTTTATCTCTCa                  >  2:1434457/1‑76 (MQ=255)
                                                          gCTGGTTAATGGCAGGGGGTCTGATCGCCTGGTTTGTCGGTTGGCGCAAAACACGCTGATTTTTTCATCGCTCCAg                >  1:1442158/1‑76 (MQ=255)
                                                           cTGGTTAATGGCAGGTGGTCTGATCGCCTGGTTTGTCGGTTGGGGCAAAAAACGCTGATTTTTTCATCGCTCCAgg               >  2:372043/1‑76 (MQ=255)
                                                           cTGGTTAATGGCAGGGGGTCTGATCGCCTGGTTTGTCGGTTGGCGCAAAACACGGTGGTTTTTTTATCGCTCAAgg               >  2:238366/1‑76 (MQ=255)
                                                            tGGTTAATGGCAGGTGGTCTGATCGCCTGGTTTGTCGGTTGGCGCAAAACACGCTGATTTTTTCATCGCTCAAGGc              >  2:185441/1‑76 (MQ=255)
                                                             ggTTAATGGCAGGTGGTCTGATCGCCTGGTTTGTCGGTTGGCGCAAAACACGCTGATTTTTTCATCGCTCAAGGCg             >  2:574446/1‑76 (MQ=255)
                                                             ggTTAATGGCAGGTGGTCTGATCGCCTGGTTTGTCGGTTGGCGCAAAACACGCTGATTTTTTCATCGCTCAAGGCg             <  2:387877/76‑1 (MQ=255)
                                                              gTTAATGGCAGGTGGTCTGATCGCCTGGTTTGTCGGTTGGCGCAAAACACGCTGATTTTTTCATCGCTCAAGGCgg            <  2:436456/76‑1 (MQ=255)
                                                                  aTGGCAGGTGGTCTGATCGCCTGGTTTGTCGGTTGGCGCAAAACACGCTGATTTTTTCATCGCTCAAGGCGGGCCg        <  2:664042/76‑1 (MQ=255)
                                                                    ggCAGGTGGTCTGATCGCCTGGTTTGTCGGTTGGCGCAAAACACGCTGATTTTTTCATCGCTCAAGGCGGGCCgtg      <  2:803837/76‑1 (MQ=255)
                                                                     gCAGGTGGTCTGATCGCCTGGTTTGTCGGTTGGCGCAAAACACGCTGATTTTTTCATCGCTCAAGGCGGGCCgtgt     <  1:1373351/76‑1 (MQ=255)
                                                                      cAGGTGGTCTGATCGCCTGGTTTGTCGGTTGGCGCAAAACACGCTGATTTTTTCATCGCTCAAGGCGGGCCGTGTa    >  2:739343/1‑76 (MQ=255)
                                                                      cAGGTGGTCTGATCGCCTGGTTTGTCGGTTGGCGCAAAACACGCTGATTTTTTCATCGCTCAAGGCGGGCCGTGTa    <  1:764413/76‑1 (MQ=255)
                                                                       cggtggtCTGAGCGCCTGGTTTGTCGGTTGGCGCAAAACACGCTGATTTTTTCATCGCTCAAGGCGGGCCGTGTaa   <  2:861160/75‑1 (MQ=255)
                                                                       aGGTGGTCTGGTCGCCTGGTTTGTCGGTTGGCGCAAAACACGCTGATTTTTTCATCGCTCAAGGCGGGCCGTGTaa   <  2:861148/76‑1 (MQ=255)
                                                                       aGGTGGTCTGATCGCCTGGTTTGTCGGTTGGCGCAAAACACGCTGATTTTTTCATCGCTCAAGGCGGGCCGTGTaa   >  2:1659445/1‑76 (MQ=255)
                                                                       aGGTGGTCTGATCGCCTGGTTTGTCGGTTGGCGCAAAACACGCTGATTTTTTCATCGCTCAAGGCGGGCCGTGTaa   <  2:1618686/76‑1 (MQ=255)
                                                                        ggtggtCTGATCGCCTGGTTTGTCGGTTGGCGCAAAACACGCTGATTTTTTCATCGCTCAAGGCGGGCCGTGTAAc  <  1:1114527/76‑1 (MQ=255)
                                                                          |                                                                         
TTAGTATTAAGTGGCACATTCTTGTTGGTCAGCCGACCTGAATGGGGGCTGATGCCCGGCTGGTTAATGGCAGGTGGTCTGATCGCCTGGTTTGTCGGTTGGCGCAAAACACGCTGATTTTTTCATCGCTCAAGGCGGGCCGTGTAAC  >  REL606/4000221‑4000368

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: