| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 2,686,827 | C→T | A292V (GCC→GTC) | gabD → | succinate‑semialdehyde dehydrogenase I, NADP‑dependent |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 2,686,827 | 0 | C | T | 96.5% | 100.8 / ‑3.7 | 29 | A292V (GCC→GTC) | gabD | succinate‑semialdehyde dehydrogenase I, NADP‑dependent |
| Reads supporting (aligned to +/- strand): ref base C (1/0); new base T (11/17); total (12/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.14e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.54e-01 | |||||||||||
GCCGACCTCGACAAAGCCGTGGAAGGTGCGCTGTCCTCGAAATTCCGCAACGCCGGGCAAACCTGCGTCTGCGCCAACCGTTTATACGTGCAGGACGGCGTGTATGACCGCTTTGCCGAAAAATTGCAGCAGGCGGTGA > REL606/2686754‑2686892 | gCCGACCTCGACAAAGCCGTGGAAGGTGCGCTGTCCTCGAAATTCCGCAACGCCGGGCAAACCTGCGTCTGCGTCa > 1:491756/1‑76 (MQ=255) ccGACCTCGACAAAGCCGTGGAAGGTGCGCTGTCCTCGAAATTCCGCAACGCCGGGCAAACCTGCGTCTGCGTCaa < 1:698977/76‑1 (MQ=255) gACAAAGCCGTGGAAGGTGCGCTGTCCTCGAAATTCCGCAACGCCGGGCAAACCTGCGTCTGCGTCAACCGTTtat < 2:1233902/76‑1 (MQ=255) aGCCGTGGAAGGTGCGCTGTCCTCGAAATTCCGCAACGCCGGGCAAACCTGCGTCTGCGTCAACCGTTTATACGTg < 2:1862672/76‑1 (MQ=255) aGCCGTGGAAGGTGCGCTGTCCTCGAAATTCCGCAACGCCGGGCAAACCTGCGTCTGCGTCAACCGTTTATACGTg < 2:1064052/76‑1 (MQ=255) aGCCGGGGAAGGTGCGCTGTCCTCGAAATTCCGCAACGCCGGGCAAACCTGCGTCTGCGTCAACCGTTTATACGTg < 2:660785/76‑1 (MQ=255) gTGGAAGGTGCGCTGTCCTCGAAATTCCGCAACGCCGGGCAAACCTGCGTCTGCGCCAACCGTTTATACGTGCAgg > 1:345310/1‑76 (MQ=255) ggAAGGTGCGCTGTCCTCGAAATTCCGCAACGCCGGGCAAACCTGCGTCTGCGTCAACCGTTTATACGTGCAGGAc > 1:303093/1‑76 (MQ=255) gAAGGTGCGCTGTCCTCGAAATTCCGCAACGCCGGGCAAACCTGCGTCTGCGTCAACCGTTTATACGTGCAGGACg < 2:1770585/76‑1 (MQ=255) aGGGGCGCTGTCCTCGAAATTCCGCAACGCCGGGCAAACCTGCGTCTGCGTCAACCGTTTATACGTGCAGGACGGc < 2:998335/76‑1 (MQ=255) tGCGCTGTCCTCGAAATTCCGCAACGCCGGGCAAACCTGCGTCTGCGTCAACCGTTTATACGTGCAGGACGGCgtg < 2:597657/76‑1 (MQ=255) cTGTCCTCGAAATTCCGCAACGCCGGGCAAACCTGCGTCTGCGTCAACCGTTTATACGTGCAGGACGGCGTGTATg > 1:1203943/1‑76 (MQ=255) tGTCCTCGAAATTCCGCAACGCCGGGCAAACCTGCGTCTGCGTCAACCGTTTATACGTGCAGGACGGCGTGTATGa < 1:466550/76‑1 (MQ=255) tGTCCTCGAAATTCCGCAACGCCGGGCAAACCTGCGTCTGCGTCAACCGTTTATACGTGCAGGACGGCGTGTATGa > 2:991569/1‑76 (MQ=255) gTCCTCGAAATTCCGCAACGCCGGGCAAACCTGCGTCTGCGTCAACCGTTTATACGTGCAGGACGGCGTGTATGAc > 1:1266220/1‑76 (MQ=255) tCCTCGAAATTCCGCAACGCCGGGCAAACCTGCGTCTGCGTCAACCGTTTATACGTGCAGGACGGCGTGTATGAcc < 1:928332/76‑1 (MQ=255) ccTCGAAATTCCGCAACGCCGGGCAAACCTGCGTCTGCGTCAACCGTTTATACGTGCAGGACGGCGTGTATGACCg > 1:1848142/1‑76 (MQ=255) cGAAATTCCGCAACGCCGGGCAAACCTGCGTCTGCGTCAACCGTTTATACGTGCAGGACGGCGTGTATGACCGCtt > 2:1279422/1‑76 (MQ=255) cGAAATTCCGCAACGCCGGGCAAACCTGCGTCTGCGTCAACCGTTTATACGTGCAGGACGGCGTGTATGACCGCtt < 2:3009/76‑1 (MQ=255) aaaTTCCGCAACGCCGGGCAAACCTGCGTCTGCGTCAACCGTTTATACGTGCAGGACGGCGTGTATGACCGCTTTg < 1:1395803/76‑1 (MQ=255) ttCCGCAACGCCGGGCAAACCTGCGTCTGCGTCAACCGTTTATACGTGCAGGACGGCGTGTATGACCGCTTTGCCg > 2:1069998/1‑76 (MQ=255) gCCGGGCAAACCTGCGTCTGCGTCAACCGTTTATACGTGCAGGACGGCGTGTATGACCGCTTTGCCGAAAAATTgc < 2:638456/76‑1 (MQ=255) cGGGCAAACCTGCGTCTGCGTCAACCGTTTATACGTGCAGGACGGCGTGTATGACCGCTTTGCCGAAAAATTgcag < 2:1564572/76‑1 (MQ=255) cGGGCAAACCTGCGTCTGCGTCAACCGTTTATACGTGCAGGACGGCGTGTATGACCGCTTTGCCGAAAAATTgcag > 1:1275161/1‑76 (MQ=255) ggCAAACCTGCGTCTGCGTCAACCGTTTATACGTGCAGGACGGCGTGTATGACCGCTTTGCCGAAAAATTgcagca < 1:193036/76‑1 (MQ=255) aaaCCTGCGTCTGCGTCAACCGTTTATACGTGCAGGACGGCGTGTATGACCGCTTTGCCGAAAAATTGCAGCAGGc > 2:903634/1‑76 (MQ=255) aaaCCTGCGTCTGCGTCAACCGTTTATACGTGCAGGACGGCGTGTATGACCGCTTTGCCGAAAAATTGCAGCAGGc < 1:1026435/76‑1 (MQ=255) aaaCCTGCGTCTGCGTCAACCGTTTATACGTACAGGACGGCGTGTATGACCGCTTTGCCGAAAAATTGCAGCAGGc < 2:845293/76‑1 (MQ=255) aaaCCTGAGTCTGCGTCAACCGTTTATACGTGCAGGACGGCGTGTATGACCGCTTTGCCGAAAAATTGCAGCAGGc > 2:861240/1‑76 (MQ=255) ctgcgtctgcgtCAACCGTTTATACGTGCAGGACGGCGTGTATGACCGCTTTGCCGAAAAATTGCAGCAGGCGGTg < 2:521197/76‑1 (MQ=255) tgcgtctgcgtcAACCGTTTATACGTGCAGGACGGCGTGTATGACCGCTTTGCCGAAAAATTGCAGCAGGCGGTGa < 2:352504/76‑1 (MQ=255) | GCCGACCTCGACAAAGCCGTGGAAGGTGCGCTGTCCTCGAAATTCCGCAACGCCGGGCAAACCTGCGTCTGCGCCAACCGTTTATACGTGCAGGACGGCGTGTATGACCGCTTTGCCGAAAAATTGCAGCAGGCGGTGA > REL606/2686754‑2686892 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |