| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 4,201,934 | T→G | Y209S (TAT→TCT) | iclR ← | DNA‑binding transcriptional repressor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 4,201,934 | 0 | T | G | 95.5% | 262.5 / 2.1 | 66 | Y209S (TAT→TCT) | iclR | DNA‑binding transcriptional repressor |
| Reads supporting (aligned to +/- strand): ref base T (1/2); new base G (37/26); total (38/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.70e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.84e-01 | |||||||||||
CACGGTGCTCATCGAAAATACACGCTGCAAGGCAACGTAGCCCCAGCGCATGTTCCTCATCGTCAAATGAATAACCCCGTTTGCGCGTTTGGGCGAGATCTTCTTTTAAATGCACAGGAGACACCAGCGTTGCGTGGGTATAGG > REL606/4201863‑4202006 | cACGGTGCTCATCGAAAATACACGCTGCAAGGCAACGTAGCCCCAGCGCATGTTCCTCATCGTCAAATGAAGAAcc > 2:757536/1‑76 (MQ=255) cGGTGCTCATCGAAAATACACGCTGCAAGGCAACGTAGCCCCAGCGCATGTTCCTCATCGTCAAATGAAGAAcccc < 1:113680/76‑1 (MQ=255) ggTGCTCATCGAAAATACACGCTGCAAGGCAACGTAGCCCCAGCGCATGTTCCTCATCGTCAAATGAAGAACCCCg < 1:1962241/76‑1 (MQ=255) gCTCATCGAAAATACACGCTGCAAGGCAACGTAGCCCCAGCGCATGTTCCTCATCGTCAAATGAAGAACCCCGttt > 1:411504/1‑76 (MQ=255) gCTCATCGAAAATACACGCTGCAAGGCAACGTAGCCCCAGCGCATGTTCCTCATCGTCAAATGAAGAACCCCGttt > 1:1583699/1‑76 (MQ=255) gCTCATCGAAAATACACGCTGCAAGGCAACGTAGCCCCAGCGCATGTTCCTCATCGTCAAATGAAGAACCCCGttt < 2:1964516/76‑1 (MQ=255) cTCATCGAAAATACACGCTGCAAGGCAACGTAGCCCCAGCGCATGTTCCTCATCGTCAAATGAAGAACCCCGTTTg > 2:327796/1‑76 (MQ=255) tCATCGAAAATACACGCTGCAAGGCAACGTAGCCCCAGCGCATGTTCCTCATCGTCAAATGAATAACCCCGTTTgc > 1:1397856/1‑76 (MQ=255) tCATCGAAAATACACGCTGCAAGGCAACGTAGCCCCAGCGCATGTTCCTCATCGTCAAATGAAGAACCCCGTTTgc > 1:978611/1‑76 (MQ=255) cATCGAAAATACACGCTGCAAGGCAACGTAGCCCCAGCGCATGTTCCTCATCGTCAAATGAAGAACCCCGTTTgcg > 2:461282/1‑76 (MQ=255) cATCGAAAATACACGCTGCAAGGCAACGTAGCCCCAGCGCATGTTCCTCATCGTCAAATGAAGAACCCCGTTTgcg > 2:1691960/1‑76 (MQ=255) aTCGAAAATACACGCTGCAAGGCAACGTAGCCCCAGCGCATGTTCCTCATCGTCAAATGAAGAACCCCGTTTgcgc > 2:1693232/1‑76 (MQ=255) tCGAAAATACACGCTGCAAGGCAACGTAGCCCCAGCGCATGTTCCTCATCGTCAAATGAAGAACCCCGTTTGcgcg > 2:1676058/1‑76 (MQ=255) gAAAATACACGCTGCAAGGCAACGTAGCCCCAGCGCATGTTCCTCATCGTCAAATGAAGAACCCCGTTTGCGCGtt > 1:434590/1‑76 (MQ=255) aaaTACACGCTGCAAGGCAACGTAGCCCCAGCGCATGTTCCTCATCGTCAAATGAAGAACCCCGTTTGCGCGTTTg > 1:13158/1‑76 (MQ=255) aaaTACACGCTGCAAGGCAACGTAGCCCCAGCGCATGTTCCTCATCGTCAAATGAAGAACCCCGTTTGCGCGTTTg < 2:389550/76‑1 (MQ=255) aTACACGCTGCAAGGCAACGTAGCCCCAGCGCATGTTCCTCATCGTCAAATGAAGAACCCCGTTTGCGCGTTTggg > 2:434526/1‑76 (MQ=255) tcgcGCTGCAGGGCAGCGTAGCCCCAGCGCATGTTCCTCATCGTCAAATGAAGAACCCCGTTTGCGCGTTTGGGCg < 2:1521128/73‑1 (MQ=255) acacGCTGCAAGGCAACGTAGCCCCAGCGCATGTTCCTCATCGTCAAATGAAGAACCCCGTTTGCGCGTTTGGGCg < 1:231175/76‑1 (MQ=255) gCTGCAAGGCAACGTAGCCCCAGCGCATGTTCCTCATCGTCAAATGAAGAACCCCGTTTGCGCGTTTGGGCGAGAt > 1:620724/1‑76 (MQ=255) cTGCAAGGCAACGTAGCCCCAGCGCATGTTCCTCATCGTCAAATGAAGAACCCCGTTTGCGCGTTTGGGCGAGAtc > 1:718495/1‑76 (MQ=255) tGCAAGGCAACGTAGCCCCAGCGCATGTTCCTCATCGTCAAATGAAGAACCCCGTTTGCGCGTTTGGGCGAGAtct > 1:339016/1‑76 (MQ=255) tGCAAGGCAACGTAGCCCCAGCGCATGTTCCTCATCGTCAAATGAAGAACCCCGTTTGCGCGTTTGGGCGAGAtct > 2:742289/1‑76 (MQ=255) gCAAGGCAACGTAGCCCCAGCGCATGTTCCTCATCGTCAAATGAAGAACCCCGTTTGCGCGTTTGGGCGCGAtctt > 1:532650/1‑76 (MQ=255) gCAAGGCAACGTAGCCCCAGCGCATGTTCCTCATCGTCAAATGAAGAACCCCGTTTGCGCGTTTGGGCGAGAtctt > 2:192696/1‑76 (MQ=255) gCAAGGCAACGTAGCCCCAGCGCATGTTCCTCATCGTCAAATGAAGAACCCCGTTTGCGCGTTTGGGCGAGAtctt > 1:665748/1‑76 (MQ=255) cAAGGCAACGTAGCCCCAGCGCATGTTCCTCATCGTCAAATGAAGAACCCCGTTTGCGCGTTTGGGCGAGAtcttc > 2:1519073/1‑76 (MQ=255) aaGGCAACGTAGCCCCAGCGCATGTTCCTCATCGTCAAATGAAGAACCCCGTTTGCGCGTTTGGGCGAGAtcttct < 2:650442/76‑1 (MQ=255) aGGCAACGTAGCCCCAGCGCATGTTCCTCATCGTCAAATGAAGAACCCCGTTTGCGCGTTTGGGCGAGATcttctt < 2:852487/76‑1 (MQ=255) aGGCAACGTAGCCCCAGCGCATGTTCCTCATCGTCAAATGAAGAACCCCGTTTGCGCGTTTGGGCGAGATcttctt > 2:194708/1‑76 (MQ=255) aGGCAACGTAGCCCCAGCGCATGTTCCTCATCGTCAAATGAAGAACCCCGTTTGCGCGTTTGGGCGAGATcttctt < 2:1834499/76‑1 (MQ=255) cAACGTAGCCCCAGCGCATGTTCCTCATCGTCAAATGAAGAACCCCGTTTGCGCGTTTGGGCGAGATCTTCTTTTa < 2:49825/76‑1 (MQ=255) aaCGTAGCCCCAGCGCATGTTCCTCATCGTCAAATGAATAACCCCGTTTGCGCGTTTGGGCGAGATCTTCTTTTaa < 1:1868445/76‑1 (MQ=255) aaCGTAGCCCCAGCGCATGTTCCTCATCGTCAAATGAAGAACCCCGTTTGCGCGTTTGGGCGAGATCTTCTTTTaa > 2:413530/1‑76 (MQ=255) cGTAGCCCCAGCGCATGTTCCTCATCGTCAAATGAAGAACCCCGTTTGCGCGTTTGGGCGAGATCTTCTTTTAAAt > 1:813960/1‑76 (MQ=255) gTAGCCCCAGCGCATGTTCCTCATCGTCAAATGAAGAACCCCGTTTGCGCGTTTGGGCGAGATCTTCTTTTAAATg > 2:410101/1‑76 (MQ=255) tAGCCCCAGCGCATGTTCCTCATCGTCAAATGAAGAACCCCGTTTGCGCGTTTGGGCGAGATCTTCTTTTAAATGc > 2:895986/1‑76 (MQ=255) aGCCCCAGCGCATGTTCCTCATCGTCAAATGAAGAACCCCGTTTGCGCGTTTGGGCGAGATCTTCTTTTAAATGca > 1:1947902/1‑76 (MQ=255) gCCCCAGCGCATGTTCCTCATCGTCAAATGAAGAACCCCGTTTGCGCGTTTGGGCGAGATCTTCTTTTAAATGcac < 1:270423/76‑1 (MQ=255) ccccAGCGCATGTTCCTCATCGTCAAATGAAGAACCCCGTTTGCGCGTTTGGGCGCGATCTTCTTTTAAATGcaca > 2:1536327/1‑76 (MQ=255) ccAGCGCATGTTCCTCATCGTCAAATGAAGAACCCCGTTTGCGCGTTTGGGCGAGATCTTCTTTTAAATGCACAgg < 2:1814157/76‑1 (MQ=255) ccAGCGCATGTTCCTCATCGTCAAATGAAGAACCCCGTTTGCGCGTTTGGGCGAGATCTTCTTTTAAATGCACAgg < 1:418428/76‑1 (MQ=255) cAGCGCATGTTCCTCATCGTCAAATGAAGAACCCCGTTTGCGCGTTTGGGCGAGATCTTCTTTTAAATGCACAGga > 1:75940/1‑76 (MQ=255) aGCGCATGTTCCTCATCGTCAAATGAAGAACCCCGTTTGCGCGTTTGGGCGAGATCTTCTTTTAAATGCACAGgag < 2:1505642/76‑1 (MQ=255) aGCGCATGTTCCTCATCGTCAAATGAAGAACCCCGTTTGCGCGTTTGGGCGAGATCTTCTTTTAAATGCACAGgag > 2:858706/1‑76 (MQ=255) aGCGCATGTTCCTCATCGTCAAATGAAGAACCCCGTTTGCGCGTTTGGGCGAGATCTTCTTTTAAATGCACAGgag < 1:1412143/76‑1 (MQ=255) gcgcATGTTCCTCATCGTCAAATGAAGAACCCCGTTTGCGCGTTTGGGCGAGATCTTCTTTTAAATGCACAGgaga < 2:560641/76‑1 (MQ=255) gcATGTTCCTCATCGTCAAATGAAGAACCCCGTTTGCGCGTTTGGGCGAGATCTTCTTTTAAATGCACAGGAGaca < 1:1829807/76‑1 (MQ=255) aTGTTCCTCATCGTCAAATGAAGAACCCCGTTTGCGCGTTTGGGCGAGATCTTCTTTTAAATGCACAGGAGACAcc < 2:270171/76‑1 (MQ=255) aTGTTCCTCATCGTCAAATGAAGAACCCCGTTTGCGCGTTTGGGCGAGATCTTCTTTTAAATGCACAGGAGACAcc > 1:1108081/1‑76 (MQ=255) aTGTTCCTCATCGTCAAATGAAGAACCCCGTTTGCGCGTTTGGGCGAGATCTTCTTTTAAATGCACAGGAGACAcc > 1:1838459/1‑76 (MQ=255) tGTTCCTCATCGTCAAATGAAGAACCCCGTTTGCGCGTTTGGGCGAGATCTTCTTTTAAATGCACAGGAGACACCa > 2:1342420/1‑76 (MQ=255) gTTCCTCATCGTCAAATGAAGAACCCCGTTTGCGCGTTTGGGCGAGATCTTCTTTTAAATGCACAGGAGACACCAg > 1:506740/1‑76 (MQ=255) gTTCCTCATCGTCAAATGAAGAACCCCGTTTGCGCGTTTGGGCGAGATCTTCTTTTAAATGCACAGGAGACACCAg > 2:472849/1‑76 (MQ=255) tCCTCATCGTCAAATGAAGAACCCCGTTTGCGCGTTTGGGCGAGATCTTCTTTTAAATGCACAGGAGACACCAGCg < 1:1587153/76‑1 (MQ=255) tCATCGTCAAATGAAGAACCCCGTTTGCGCGTTTGGGCGAGATCTTCTTTTAAATGCACAGGAGACACCAGCGTTg < 2:1024511/76‑1 (MQ=255) cATCGTCAAATGAAGAACCCCGTTTGCGCGTTTGGGCGAGATCTTCTTTTAAATGCACAGGAGACACCAGCGTTGc > 1:100571/1‑76 (MQ=255) aTCGTCAAATGAAGAACCCCGTTTGCGCGTTTGGGCGAGATCTTCTTTTAAATGCACAGGAGACACCAGCGTTGCg > 1:443806/1‑76 (MQ=255) tCGTCAAATGAAGAACCCCGTTTGCGCGTTTGGGCGAGATCTTCTTTTAAATGCACAGGAGACACCAGCGTTGCGt < 1:677390/76‑1 (MQ=255) cGTCAAATGAAGAACCCCGTTTGCGCGTTTGGGCGAGATCTTCTTTTAAATGCACAGGAGACACCAGCGTTGCGTg > 2:1403463/1‑76 (MQ=255) cGTCAAATGAAGAACCCCGTTTGCGCGTTTGGGCGAGATCTTCTTTTAAATGCACAGGAGACACCAGCGTTGCGTg < 1:1068831/76‑1 (MQ=255) gTCAAATGAATAACCCCGTTTGCGCGTTTGGGCGAGATCTTCTTTTAAATGCACAGGAGACACCAGCGTTGCGTgg < 1:355813/76‑1 (MQ=255) gTCAAATGAAGAACCCCGTTTGCGCGTTTGGGCGAGATCTTCTTTTAAATGCACAGGAGACACCAGCGTTGCGTgg < 2:459903/76‑1 (MQ=255) gTCAAATGAAGAACCCCGTTTGCGCGTTTGGGCGAGATCTTCTTTTAAATGCACAGGAGACACCAGCGTTGCGTgg < 1:1295501/76‑1 (MQ=255) cAAATGAAGAACCCCGTTTGCGCGTTTGGGCGAGATCTTCTTTTAAATGCACAGGAGACACCAGCGTTGCGTGGGt < 2:339016/76‑1 (MQ=255) aaaTGAAGAACCCCGTTTGCGGGTTTGGGCGAGATCTTCTTTTAAATGCACAGGAGCCACCAGCGTTGCGGGGGtt > 2:497288/1‑75 (MQ=255) aaTGAAGAACCCCGTTTGCGCGTTTGGGCGAGATCTTCTTTTAAATGCACAGGAGACACCAGCGTTGCGTGGGtat < 1:854074/76‑1 (MQ=255) gaagaaCCCCGTTTGCGCGTTTGGGCGAGATCTTCTTTTAAATGCACAGGAGACACCAGCGTTGCGTGGGTATAgg < 1:973255/76‑1 (MQ=255) | CACGGTGCTCATCGAAAATACACGCTGCAAGGCAACGTAGCCCCAGCGCATGTTCCTCATCGTCAAATGAATAACCCCGTTTGCGCGTTTGGGCGAGATCTTCTTTTAAATGCACAGGAGACACCAGCGTTGCGTGGGTATAGG > REL606/4201863‑4202006 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |