| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 4,520,058 | T→C | intergenic (‑419/‑1037) | yjhA ← / → fimB | N‑acetylnuraminic acid outer membrane channel protein/recombinase involved in phase variation; regulator for fimA; b4312_1 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 4,520,058 | 0 | T | C | 82.3% | 190.5 / 28.7 | 68 | intergenic (‑419/‑1037) | yjhA/fimB | N‑acetylnuraminic acid outer membrane channel protein/recombinase involved in phase variation; regulator for fimA; b4312_1 |
| Reads supporting (aligned to +/- strand): ref base T (5/7); new base C (28/28); total (33/35) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.53e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01 | |||||||||||
CACCCGATGAGAAAGAACTGAAGTGATGTTTGCCATAGATTGCGAGATGCCCTGTCTCATTGACTATCTTGGTGCACCTTTATACCTGTTATACCAGATCAAAAATCACGCAATCCATACAACAAAACCAGATTTGCAATTCGTGTCA > REL606/4519986‑4520133 | caCCCGATGAGAAAGAACTGAAGTGATGTTTGCCATAGATTGCGAGATACCCTGTCTCATTGACTATCTTGGCGCa > 2:305038/1‑76 (MQ=255)caCCCGATGAGAAAGAACTGAAGTGATGTTTGCCATAGATTGCGAGATACCCTGTCTCATTGACTATCTTGGCGCa > 2:305065/1‑76 (MQ=255) caaTGAGAAAGAACTGAAGTGATGTTTGCCATAGATTGCGAGATGCCCTGTCTCATTTACTATCTTGGCGCACAtt < 2:744395/74‑1 (MQ=255) cGATGAGAAAGAACTGAAGTGATGTTTGCCATAGATTGCGAGATGCCCTGTCTCATTGACTATCTTGGCGCACCtt < 2:744359/76‑1 (MQ=255) gATGAGAAAGAACTGAAGTGATGTTTGCCATAGATTGCGAGATGCCCTGTCTCATTGACTATCTTGGTGCACCttt < 1:312491/76‑1 (MQ=255) gATGAGAAAGAACTGAAGTGATGTTTGCCATAGATTGCGAGATGCCCTGTCTCATTGACTATCTTGGCGCACCttt > 2:165746/1‑76 (MQ=255) tGAGAAAGAACTGAAGTGATGTTTGCCATAGATTGCGAGATGCCCTGTCTCATTGACTATCTTGGCGCACCTTtat < 1:792321/76‑1 (MQ=255) tGAGAAAGAACTGAAGTGATGTTTGCCATAGATTGCGAGATGCCCTGTCTCATTGACTATCTTGGCGCACCTTtat < 1:1492231/76‑1 (MQ=255) tGAGAAAGAACTGAAGTGATGTTTGCCATAGATTGCGAGATGCCCTGTCTCATTGACTATCTTGGCGCACCTTtat < 2:1724055/76‑1 (MQ=255) gagaAAGAACTGAAGTGATGTTTGCCATAGATTGCGAGATGCCCTGTCTCATTGACTATCTTGGCGCACCTTtata > 2:176111/1‑76 (MQ=255) agaaagaaCTGAAGTGATGTTTGCCATAGATTGCGAGATGCCCTGTCTCATTGACTATCTTGGCGCACCTTTATAc > 1:1285716/1‑76 (MQ=255) gaaagaaCTGAAGTGATGTTTGCCATAGATTGCGAGATGCCCTGTCTCATTGACTATCTTGGCGCACCTTTATAcc < 2:1448734/76‑1 (MQ=255) aaagaaCTGAAGTGATGTTTGCCATAGATTGCGAGATGCCCTGTCTCATTGACTATCTTGGCGCACCTTTATACCt < 1:1115889/76‑1 (MQ=255) aaagaaCTGAAGTGATGTTTGCCATAGATTGCGAGATGCCCTGTCTCATTGACTATCTTGGCGCACCTTTATACCt < 2:209578/76‑1 (MQ=255) aagaaCTGAAGTGATGTTTGCCATAGATTGCGAGATGCCCTGTCTCATTGACTATCTTGGCGCACCTTTATACCTg < 2:1611352/76‑1 (MQ=255) agaaCTGAAGTGATGTTTGCCATAGATTGCGAGATGCCCTGTCTCATTGACTATCTTGGTGCACCTTTATACCTGt < 1:161369/76‑1 (MQ=255) cTGAAGTGATGTTTGCCATAGATTGCGAGATGCCCTGTCTCATTGACTATCTTGGCGCACCTTTATACCTGTtata > 1:1747737/1‑76 (MQ=255) gAAGTGATGTTTGCCATAGATTGCGAGATGCCCTGTCTCATTGACTATCTTGGCGCACCTTTATACCTGTTATAcc > 2:41324/1‑76 (MQ=255) gAAGTGATGTTTGCCATAGATTGCGAGATGCCCTGTCTCATTGACTATCTTGGCGCACCTTTATACCTGTTATAcc > 1:293939/1‑76 (MQ=255) gAAGTGATGTTTGCCATAGATTGCGAGATGCCCTGTCTCATTGACTATCTTGGCGCACCTTTATACCTGTTATAcc > 2:1971866/1‑76 (MQ=255) gAAGTGATGTTTGCCATAGATTGCGAGATGCCCTGTCTCATTGACTATCTTGGCGCACCTTTATACCTGTTATAcc > 2:1452870/1‑76 (MQ=255) caaGTGATGTTTGCCATAGATTGCGAGATGCCCTGTCTCATTGACTATCTTGGTGCACCTTTATACCTGTTATccc < 2:326355/75‑1 (MQ=255) aaGTGATGTTTGCCATAGATTGCGAGATGCCCTGTCTCATTGACTATCTTGGCGCACCTTTATACCTGTTATACCa > 2:1675458/1‑76 (MQ=255) gATGTTTGCCATAGATTGCGAGATGCCCTGTCTCATTGACTATCTTGGCGCACCTTTATACCTGTTATACCAGATc < 1:235175/76‑1 (MQ=255) gTTTGCCATAGATTGCGAGATGCCCTGTCTCATTGACTATCTTGGCGCACCTTTATACCTGTTATACCAGATCaaa > 1:1484673/1‑76 (MQ=255) gTTTGCCATAGATTGCGAGATGCACTGTCTCATTGACTATCTTGGTGCACCTTTATACCTGTTATACCAGATCaaa > 2:885227/1‑76 (MQ=255) tttGCCATAGATTGCGAGATGCCCTGTCTCATTGACTATCTTGGCGCACCTTTATACCTGTTATACCAGATCaaaa < 1:939930/76‑1 (MQ=255) tGCCATAGATTGCGAGATGCCCTGTCTCATTGACTATCTTGGTGCACCTTTATACCTGTTATACCAGATCAAAAAt > 2:891800/1‑76 (MQ=255) gCCATAGATTGCGAGATGCCCTGTCTCATTGACTATCTTGGCGCACCTTTATACCTGTTATACCAGATCAAAAATc < 2:697271/76‑1 (MQ=255) cATAGATTGCGAGATGCCCTGTCTCATTGACTATCTTGGCGCACCTTTATACCTGTTATACCAGATCAAAAATCAc < 1:150265/76‑1 (MQ=255) tAGATTGCGAGATGCCCTGTCTCATTGACTATCTTGGCGCACCTTTATACCTGTTATACCAGATCAAAAATCACGc > 2:832183/1‑76 (MQ=255) aGATTGCGAGATGCCCTGTCTCATTGACTATCTTGGCGCACCTTTATACCTGTTATACCAGATCAAAAATCACGCa > 2:837098/1‑76 (MQ=255) gATTGCGAGATGCCCTGTCTCATTGACTATCTTGGCGCACCTTTATACCTGTTATACCAGATCAAAAATCACGCaa > 1:1658597/1‑76 (MQ=255) ttGCGAGATGCCCCGTCTCATTGACTATCTTGGTGCACCTTTATACCTGTTATACCAGATCAAAAATCACGCAATc < 2:1483549/76‑1 (MQ=255) tGCGAGATGCCCTGTCTCATTGACTATCTTGGCGCACCTTTATACCTGTTATACCAGATCAAAAATCACGCAATcc < 2:1190757/76‑1 (MQ=255) tGCGAGATGCCCTGTCTCATTGACTATCTTGGCGCACCTTTATACCTGTTATACCAGATCAAAAATCACGCAATcc > 1:287403/1‑76 (MQ=255) tGCGAGATGCCCTGTCTCATTGACTATCTTGGCGCACCTTTATACCTGTTATACCAGATCAAAAATCACGCAATcc > 2:1054124/1‑76 (MQ=255) cGAGATGCCCTGTCTCATTGACTATCTTGGCGCACCTTTATACCTGTTATACCAGATCAAAAATCACGCAATCCAt < 2:544567/76‑1 (MQ=255) cGAGATGCCCTGTCTCATTGACTATCTTGGCGCACCTTTATACCTGTTATACCAGATCAAAAATCACGCAATCCAt < 1:567479/76‑1 (MQ=255) gaTGCCCTGTCTCATTGACTATCTTGGCGCACCTTTATACCTGTTATACCAGATCAAAAATCACGCAATCCATaca < 1:495357/76‑1 (MQ=255) aTGCCCTGTCTCATTGACTATCTTGGTGCACCTTTATACCTGTTATACCAGATCAAAAATCACGCAATCCATacaa < 1:1821330/76‑1 (MQ=255) aTGCCCTGTCTCATTGACTATCTTGGCGCACCTTTATACCTGTTATACCAGATCAAAAATCACGCAATCCATacaa < 2:806734/76‑1 (MQ=255) tGCCCTGTCTCATTGACTATCTTGGCGCACCTTTATACCTGTTATACCAGATCAAAAATCACGCAATCCATacaac > 1:406434/1‑76 (MQ=255) tGCCCTGTCTCATTGACTATCTTGGCGCACCTTTATACCTGTTATACCAGATCAAAAATCACGCAATCCATacaac > 1:1307148/1‑76 (MQ=255) gCCCTGTCTCATTGACTATCTTGGCGCACCTTTATACCTGTTATACCAGATCAAAAATCACGCAATCCATacaaca < 1:584159/76‑1 (MQ=255) cccTGTCTCATTGACTATCTTGGCGCACCTTTATACCTGTTATACCAGATCAAAAATCACGCAATCCATAcaacaa > 1:643839/1‑76 (MQ=255) tctcATTGACTATCTTGGCGCACCTTTATACCTGTTATACCAGATCAAAAATCACGCAATCCATACAACAAAACCa > 1:320214/1‑76 (MQ=255) ctcATTGACTATCTTGGCGCACCTTTATACCTGTTATACCAGATCAAAAATCACGCAATCTATACAACAAAACCAg > 1:1842328/1‑76 (MQ=255) ctcATTGACTATCTTGGCGCACCTTTATACCTGTTATACCAGATCAAAAATCACGCAATCCATACAACAAAACCAg > 2:882154/1‑76 (MQ=255) ctcATTGACTATCTTGGCGCACCTTTATACCTGTTATACCAGATCAAAAATCACGCAATCCATACAACAAAACCAg > 2:882169/1‑76 (MQ=255) ctcATTGACTATCTTGGCGCACCTTTATACCTGTTATACCAGATCAAAAATCACGCAATCCATACAACAAAACCAg < 1:719428/76‑1 (MQ=255) ctcATTGACTATCTTGGCGCACCTTTATACCTGTTATACCAGATCAAAAATCACGCAATCCATACAACAAAACCAg > 1:894835/1‑76 (MQ=255) tcATTGACTATCTTGGCGCACCTTTATACCTGTTATACCAGATCAAAAATCACGCAATCCATACAACAAAACCAGa < 1:176111/76‑1 (MQ=255) tcATTGACTATCTTGGCGCACCTTTATACCTGTTATACCAGATCAAAAATCACGCAATCCATACAACAAAACCAGa > 2:478213/1‑76 (MQ=255) cATTGACTATCTTGGCGCACCTTTATACCTGTTATACCAGATCAAAAATCACGCAATCCATACAACAAAACCAGAt < 2:1651933/76‑1 (MQ=255) cATTGACTATCTTGGCGCACCTTTATACCTGTTATACCAGATCAAAAATCACGCAATCCATACAACAAAACCAGAt > 1:1795465/1‑76 (MQ=255) aTTGACTATCTTGGTGCACCTTTATACCTGTTATACCAGATCAAAAATCACGCAATCCATACAACAAAACCAGAtt < 2:1106330/76‑1 (MQ=255) aTTGACTATCTTGGTGCACCTTTATACCTGTTATACCAGATCAAAAATCACGCAATCCATACAACAAAACCAGAtt < 2:1106327/76‑1 (MQ=255) ttGACTATCTTGGCGCACCTTTATACCTGTTATTCCAGATCAAAAATCACGCAATCCATACAACAAAACCAGAttt < 2:1923520/76‑1 (MQ=255) tGACTATCTTGGCGCACCTTTATACCTGTTATACCAGATCAAAAATCACGCAATCCATACAACAAAACCAGATTTg < 1:1867506/76‑1 (MQ=255) gACTATCTTGGCGCACCTTTATACCTGTTATACCAGATCAAAAATCACGCAATCCATACAACAAAACCAGATTTGc < 2:656047/76‑1 (MQ=255) aCTATCTTGGCGCACCTTTATACCTGTTATACCAGATCAAAAATCACGCAATCCATACAACAAAACCAGATTTGCa < 1:1932934/76‑1 (MQ=255) cTATCTTGGCGCACCTTTATACCTGTTATACCAGATCAAAAATCACGCAATCCATACAACAAAACCAGATTTGCaa < 1:409667/76‑1 (MQ=255) tATCTTGGCGCACCTTTATACCTGTTATACCAGATCAAAAATCACGCAATCCATACAACAAAACCAGATTTGCAAt < 2:1336353/76‑1 (MQ=255) tCTTGGTGCACCTTTATACCTGTTATACCAGATCAAAAATCACGCAATCCATACAACAAAACCAGATTTGCAATTc < 1:27083/76‑1 (MQ=255) cTTGGTGCACCTTTATACCTGTTATACCAGATCAAAAATCACGCAATCCATACAACAAAACCAGATTTGCAATTCg > 2:1123518/1‑76 (MQ=255) cTTGGCGCACCTTTATACCTGTTATACCAGATCAAAAATCACGCAATCCATACAACAAAACCAGATTTGCAATTCg < 2:128162/76‑1 (MQ=255) tGGTGCACCTTTATACCTGTTATACCAGATCAAAAATCACGCAATCCATACAACAAAACCAGATTTGCAATTCgtg > 2:585664/1‑76 (MQ=255) tGGTGCACCTTTATACCTGTTATACCAGATCAAAAATCACGCAATCCATACAACAAAACCAGATTTGCAATTCgtg > 1:1760033/1‑76 (MQ=255) tGGCGCACCTTTATACCTGTTATACCAGATCAAAAATCACGCAATCCATACAACAAAACCAGATTTGCAATTCgtg > 2:165397/1‑76 (MQ=255) tGCACCTTTATACCTGTTATACCAGATCAAAAATCACGCAATCCATACAACAAAACCAGATTTGCAATTCGTGTca < 2:996979/76‑1 (MQ=255) | CACCCGATGAGAAAGAACTGAAGTGATGTTTGCCATAGATTGCGAGATGCCCTGTCTCATTGACTATCTTGGTGCACCTTTATACCTGTTATACCAGATCAAAAATCACGCAATCCATACAACAAAACCAGATTTGCAATTCGTGTCA > REL606/4519986‑4520133 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |