Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	1,329,919	C→T	A167V (GCG→GTG)	topA →	DNA topoisomerase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	1,329,919	0	C	T	100.0%	132.6 / NA	35	A167V (GCG→GTG)	topA	DNA topoisomerase I
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (18/17);  total (18/17)

ACTAAAAACGCGATCCGCCAGGCATTTAACAAACCGGGTGAGCTGAATATTGATCGTGTTAATGCCCAGCAGGCGCGTCGCTTTATGGACCGCGTGGTGGGGTATATGGTTTCGCCGCTGCTATGGAAAAAGATCGCTCGTGGTCT  >  REL606/1329846‑1329991                                                                          |                                                                         aCTAAAAACGCGATCCGCCAGGCATTTAACAAACCGGGTGAGCTGAATATTGATCGTGTTAATGCCCAGCAGgtgc                                                                        >  1:1216022/1‑76 (MQ=255)     aaaaCGCGATCCGCCAGGCATTTAACAAACCGGGTGAGCTGAATATTGATCGTGTTAATGCCCAGCAGGTGCGTCg                                                                    <  2:673415/76‑1 (MQ=255)      aaaCGCGATCCGCCAGGCATTTAACAAACCGGGTGAGCTGAATATTGATCGTGTTAATGCCCAGCAGGTGCGTCGc                                                                   >  1:1876610/1‑76 (MQ=255)       aaCGCGATCCGCCAGGCATTTAACAAACCGGGTGAGCTGAATATTGATCGTGTTAATGCCCAGCAGGTGCGTCGCt                                                                  >  2:1186437/1‑76 (MQ=255)        aCGCGATCCGCCAGGCATTTAACAAACCGGGTGAGCTGAATATTGATCGTGTTAATGCCCAGCAGGTGCGTCGCtt                                                                 <  2:147531/76‑1 (MQ=255)         cgcgATCCGCCAGGCGTTTAACAAACCGGGTGAGCTGAATATTGATCGTGTTAATGCCCAGCAGGTGCGTCGCttt                                                                <  2:393956/76‑1 (MQ=255)         cgcgATCCGCCAGGCATTTAACAAACCGGGTGAGCTGAATATTGATCGTGTTAATGCCCAGCAGGTGCGTCGCttt                                                                <  2:1868118/76‑1 (MQ=255)             aTCCGCCAGGCATTTAACAAACCGGGTGAGCTGAATATTGATCGTGTTAATGCCCAGCAGGTGCGTCGCTTTATgg                                                            >  2:403947/1‑76 (MQ=255)                   cAGGCATTTAACAAACCGGGTGAGCTGAATATTGATCGTGTTAATGCCCAGCAGGTGCGTCGCTTTATGGACcgcg                                                      <  1:1041404/76‑1 (MQ=255)                     ggCATTTAACAAACCGGGTGAGCTGAATATTGATCGTGTTAATGCCCAGCAGGTGCGTCGCTTTATGGACCGCgtg                                                    <  2:1569343/76‑1 (MQ=255)                     ggCATTTAACAAACCGGGTGAGCTGAATATTGATCGTGTTAATGCCCAGCAGGTGCGTCGCTTTATGGACCGCgtg                                                    >  1:1260799/1‑76 (MQ=255)                         tttAACAAACCGGGTGAGCTGAATATTGATCGTGTTAATGCCCAGCAGGTGCGTCGCTTTATGGACCGCGtggtgg                                                <  1:1218332/76‑1 (MQ=255)                          tcaaCAAACCGCGTGAGCTGAATATTGATCGTGTTAATTCCCAGCAGGTGCGTCGCTTTATGGACCGCGTGGTggg                                               <  2:1967893/74‑1 (MQ=255)                            aaCAAACCGGGTGAGCTGAATATTGATCGTGTTAATGCCCAGCAGGTGCGTCGCTTTATGGACCGCGTGGTGGGGt                                             <  2:1926985/76‑1 (MQ=255)                             aCAAACCGGGTGAGCTGAATATTGATCGTGTTAATGCCCAGCAGGTGCGTCGCTTTATGGACCGCGTGGCGGGGta                                            >  2:196128/1‑76 (MQ=255)                               aaaCCGGGTGAGCTGAATATTGATCGTGTTAATGCCCAGCAGGTGCGTCGCTTTATGGACCGCGTGGTGGGGtata                                          >  2:1482610/1‑76 (MQ=255)                                   cGGGTGAGCTGAATATTGATCGTGTTAATGCCCAGCAGGTGCGTCGCTTTATGGACCGCGTGGTGGGGTATATGGt                                      <  1:1764439/76‑1 (MQ=255)                                       tGAGCTGAATATTGATCGTGTTAATGCCCAGCAGGTGCGTCGCTTTATGGACCGCGTGGTGGGGTATATGGTTTcg                                  <  2:1246002/76‑1 (MQ=255)                                        gAGCTGAATATTGATCGTGTTAATGCCCAGCAGGTGCGTCGCTTTATGGACCGCGTGGTGGGGTATATGGTTTcgc                                 >  2:125902/1‑76 (MQ=255)                                           cTGAATATTGATCGTGTTAATGCCCAGCAGGTGCGTCGCTTTATGGACCGCGTGGTGGGGTATATGGTTTcgccgc                              >  1:1334467/1‑76 (MQ=255)                                            tGAATATTGATCGTGTTAATGCCCAGCAGGTGCGTCGCTTTATGGACCGCGTGGTGGGGTATATGGTTTCGCCgct                             <  1:492986/76‑1 (MQ=255)                                              aaTATTGATCGTGTTAATGCCCAGCAGGTGCGTCGCTTTATGGACCGCGTGGTGGGGTATATGGTTTCGCCgctgc                           >  2:1332953/1‑76 (MQ=255)                                               atatTGATCGTGTTAATGCCCAGCAGGTGCGTCGCTTTATGGACCGCGTGGTGGGGTATATGGTTTCGCCgctgct                          <  2:1171466/76‑1 (MQ=255)                                                   tGATCGTGTTAATGCCCAGCAGGTGCGTCGCTTTATGGACCGCGTGGTGGGGTATATGGTTTCGCCGCTGCTATgg                      >  2:666787/1‑76 (MQ=255)                                                   tGATCGTGTTAATGCCCAGCAGGTGCGTCGCTTTATGGACCGCGTGGTGGGGTATATGGTTTCGCCGCTGCTATgg                      >  2:781420/1‑76 (MQ=255)                                                     aTCGTGTTAATGCCCAGCAGGTGCGTCGCTTTATGGACCGCGTGGTGGGGTATATGGTTTCGCCGCTGCTATGGaa                    >  1:1633090/1‑76 (MQ=255)                                                      tCGTGTTAATGCCCAGCAGGTGCGTCGCTTTATGGACCGCGTGGTGGGGTATATGGTTTCGCCGCTGCTATGGaaa                   <  2:606965/76‑1 (MQ=255)                                                       cGTGTTAATGCCCAGCAGGTGCGTCGCTTTATGGACCGCGTGGTGGGGTATATGGTTTCGCCGCTGCTATGGaaaa                  <  1:38819/76‑1 (MQ=255)                                                           ttAATGCCCAGCAGGTGCGTCGCTTTATGGACCGCGTGGTGGGGTATATGGTTTCGCCGCTGCTATGGAAAAAGAt              >  1:544320/1‑76 (MQ=255)                                                           ttAATGCCCAGCAGGTGCGTCGCTTTATGGACCGCGTGGTGGGGTATATGGTTTCGCCGCTGCTATGGAAAAAGAt              <  1:108833/76‑1 (MQ=255)                                                               tGCCCAGCAGGTGCGTCGCTTTATGGACCGCGTGGTGGGGTATATGGTTTCGCCGCTGCTATGGAAAAAGATCGCt          <  2:1264429/76‑1 (MQ=255)                                                                gCCCAGCAGGTGCGTCGCTTTATGGACCGCGTGGTGGGGTATATGGTTTCGCCGCTGCTATGGAAAAAGATCGCTc         >  1:175059/1‑76 (MQ=255)                                                                    agcagGTGCGTCGCTTTATGGACCGCGTGGTGGGGTATATGGTTTCGCCGCTGCTATGGAAAAAGATCGCTCGTgg     >  1:1264983/1‑76 (MQ=255)                                                                     gcagGTGCGTCGCTTTATGGACCGCGTGGTGGGGTATATGGTTTCGCCGCTGCTATGGAAAAAGATCGCTCGTGgt    >  1:729410/1‑76 (MQ=255)                                                                      cagGTGCGTCGCTTTATGGACCGCGTGGTGGGGTATATGGTTTCGCCGCTGTTATGGAAAAAGATCGCTCGTGgtc   >  1:889539/1‑76 (MQ=255)                                                                       agGTGCGTCGCTTTATGGACCGCGTGGTGCGGTATATGGTTTCGCCGCTGCTATGGAAAAAGATCGCTCGTGgtct  >  2:1643575/1‑76 (MQ=255)                                                                          |                                                                         ACTAAAAACGCGATCCGCCAGGCATTTAACAAACCGGGTGAGCTGAATATTGATCGTGTTAATGCCCAGCAGGCGCGTCGCTTTATGGACCGCGTGGTGGGGTATATGGTTTCGCCGCTGCTATGGAAAAAGATCGCTCGTGGTCT  >  REL606/1329846‑1329991

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑