| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 1,573,659 | A→C | D39A (GAT→GCT) | yneC → | autoinducer‑2 (AI‑2) modifying protein LsrG |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 1,573,659 | 0 | A | C | 91.7% | 190.3 / 5.0 | 48 | D39A (GAT→GCT) | yneC | autoinducer‑2 (AI‑2) modifying protein LsrG |
| Reads supporting (aligned to +/- strand): ref base A (1/3); new base C (15/29); total (16/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01 | |||||||||||
GTTGACGAGTTTATCGAAGTTTTTCGCCAGAACCACCTGGGCTCTGTACAGGAAGAAGGCAATTTGCGCTTCGATGTCTTACAGGACCCGGAAGTGAATTCGCGCTTTTATATCTACGAAGCCTATAAAGATGAAGACGCAGTGGC > REL606/1573586‑1573731 | gTTGACGAGTTTATCGAAGTTTTTCGCCAGAACCACCTGGGCTCTGTACAGGAAGAAGGCAATTTGCGCTTCGCTg < 2:1050701/76‑1 (MQ=255) tGACGAGTTTATCGAAGTTTTTCGCCAGAACCACCTGGGCTCTGTACAGGAAGAAGGCAATTTGCGCTTCGCTGTc > 1:1093882/1‑76 (MQ=255) tGACGAGTTTATCGAAGTTTTTCGCCAGAACCACCTGGGCTCTGTACAGGAAGAAGGCAATTTGCGCTTCGCTGTc > 1:655095/1‑76 (MQ=255) aCGAGTTTATCGAAGTTTTTCGCCAGAACCACCTGGGCTCTGTACAGGAAGAAGGCAATTTGCGCTTCGCTGTCtt < 1:1279123/76‑1 (MQ=255) aCGAGTTTATCGAAGTTTTTCGCCAGAACCACCTGGGCTCTGTACAGGAAGAAGGCAATTTGCGCTTCGCTGTCtt < 2:352473/76‑1 (MQ=255) cGAGTTTATCGAAGTTTTTCGCCAGAACCACCTGGGCTCTGTACAGGAAGAAGGCAATTTGCGCTTCGCTGTCTTa < 2:1673027/76‑1 (MQ=255) gAGTTTATCGAAGTTTTTCGCCAGAACCACCTGGGCTCTGTACAGGAAGAAGGCAATTTGCGCTTCGCTGTCTTAc > 1:731269/1‑76 (MQ=255) gAGTTTATCGAAGTTTTTCGCCAGAACCACCTGGGCTCTGTACAGGAAGAAGGCAATTTGCGCTTCGATGTCTTAc < 1:739803/76‑1 (MQ=255) tttATCGAAGTTTTTCGCCAGAACCACCTGGGCTCTGTACAGGAAGAAGGCAATTTGCGCTTCGCTGTCTTACAgg < 1:1476895/76‑1 (MQ=255) ttATCGAAGTTTTTCGCCAGAACCACCTGGGCTCTGTACAGGAAGAAGGCAATTTGCGCTTCGCTGTCTTACAGGa > 2:1991797/1‑76 (MQ=255) aTCGAAGTTTTTCGCCAGAACCACCTGGGCTCTGTACAGGAAGAAGGCAATTTGCGCTTCGCTGTCTTACAGGAcc > 1:143451/1‑76 (MQ=255) aTCGAAGTTTTTCGCCAGAACCACCTGGGCTCTGTACAGGAAGAAGGCAATTTGCGCTTCGCTGTCTTACAGGAcc > 1:442573/1‑76 (MQ=255) aTCGAAGTTTTTCGCCAGAACCACCTGGGCTCTGTACAGGAAGAAGGCAATTTGCGCTTCGCTGTCTTACAGGAcc < 2:383038/76‑1 (MQ=255) tCGAAGTTTTTCGCCAGAACCACCTGGGCTCTGTACAGGAAGAAGGCAATTTGCGCTTCGCTGTCTTACAGGAccc > 1:783084/1‑76 (MQ=255) cGAAGTTTTTCGCCAGAACCACCTGGGCTCTGTACAGGAAGAAGGCAATTTGCGCTTCGCTGTCTTACAGGACCCg < 2:1427534/76‑1 (MQ=255) cGAAGTTTTTCGCCAGAACCACCTGGGCTCTGTACAGGAAGAAGGCAATTTGCGCTTCGCTGTCTTACAGGACCCg > 1:835266/1‑76 (MQ=255) aGTTTTTCGCCAGAACCACCTGGGCTCTGTACAGGAAGAAGGCAATTTGCGCTTCGCTGTCTTACAGGACCCGGaa < 2:13639/76‑1 (MQ=255) ttttCGCCAGAACCACCTGGGCTCTGTACAGGAAGAAGGCAATTTGCGCTTCGCTGTCTTACAGGACCCGGAAGTg < 1:1056881/76‑1 (MQ=255) ttttCGCCAGAACCACCTGGGCTCTGTACAGGAAGAAGGCAATTTGCGCTTCGCTGTCTTACAGGACCCGGAAGTg < 1:425650/76‑1 (MQ=255) tCGCCAGAACCACCTGGGCTCTGTACAGGAAGAAGGCAATTTGCGCTTCGCTGTCTTACAGGACCCGGAAGTGAAt > 2:1957490/1‑76 (MQ=255) cGCCAGAACCACCTGGGCTCTGTACAGGAAGAAGGCAATTTGCGCTTCGCTGTCTTACAGGACCCGGAAGTGAAtt > 1:454780/1‑76 (MQ=255) cGCCAGAACCACCTGGGCTCTGTACAGGAAGAAGGCAATTTGCGCTTCGATGTCTCACAGGACCCGGAAGTGAAtt > 2:538712/1‑76 (MQ=255) aGAACCACCTGGGCTCTGTACAGGAAGAAGGCAATTTGCGCTTCGCTGTCTTACAGGACCCGGAAGTGAATTcgcg > 2:22745/1‑76 (MQ=255) accaccTGGGCTCTGTACAGGAAGAAGGCAATTTGCGCTTCGCTGTCTTACAGGACCCGGAAGTGAATTCGCGCtt < 2:1051252/76‑1 (MQ=255) accaccTGGGCTCTGTACAGGAAGAAGGCAATTTGCGCTTCGCTGTCTTACAGGACCCGGAAGTGAATTCGCGCtt < 2:1873515/76‑1 (MQ=255) caccTGGGCTCTGTACAGGAAGAAGGCAATTTGCGCTTCGCTGTCTTACAGGACCCGGAAGTGAATTCGCGCtttt < 2:616870/76‑1 (MQ=255) caccTGGGCTCTGTACAGGAAGAAGGCAATTTGCGCTTCGCTGTCTTACAGGACCCGGAAGTGAATTCGCGCtttt < 2:1269963/76‑1 (MQ=255) caccTGGGCTCTGTACAGGAAGAAGGCAATTTGCGCTTCGCTGTCTTACAGGACCCGGAAGTGAATTCGCGCtttt > 1:58438/1‑76 (MQ=255) ccTGGGCTCTGTACAGGAAGAAGGCAATTTGCGCTTCGCTGTCTTACAGGACCCGGAAGTGAATTCGCGCTTTtat < 1:445246/76‑1 (MQ=255) cTGGGCTCTGTACAGGAAGAAGGCAATTTGCGCTTCGCTGTCTTACAGGACCCGGAAGTGAATTCGCGCTTTtata < 1:331378/76‑1 (MQ=255) gggCTCTGTACAGGAAGAAGGCAATTTGCGCTTCGCTGTCTTACAGGACCCGGAAGTGAATTCGCGCTTTTatata < 2:1560919/76‑2 (MQ=255) gCTCTGTACAGGAAGAAGGCAATTTGCGCTTCGCTGTCTTACAGGACCCGGAAGTGAATTCGCGCTTTTATATCTa < 1:1208710/76‑1 (MQ=255) tctGTACAGGAAGAAGGCAATTTGCGCTTCGCTGTCTTACAGGACCCGGAAGTGAATTCGCGCTTTTATATCTACg < 2:783084/76‑1 (MQ=255) tctGTACAGGAAGAAGGCAATTTGCGCTTCGCTGTCTTACAGGACCCGGAAGTGAATTCGCGCTTTTATATCTACg < 1:620320/76‑1 (MQ=255) tctGTACAGGAAGAAGGCAATTTGCGCTTCGATGTCTTACAGGACCCGGAAGTGAATTCGCGCTTTTATATCTACg < 1:857489/76‑1 (MQ=255) ctGTACAGGAAGAAGGCAATTTGCGCTTCGCTGTCTTACAGGACCCGGAAGTGAATTCGCGCTTTTATATCTACGa < 2:460947/76‑1 (MQ=255) aCAGGAAGAAGGCAATTTGCGCTTCGCTGTCTTACAGGACCCGGAAGTGAATTCGCGCTTTTATATCTACGAAGcc > 1:1577228/1‑76 (MQ=255) cAGGAAGAAGGCAATTTGCGCTTCGCTGTCTTACAGGACCCGGAAGTGAATTCGCGCTTTTATATCTACGAAGCCt < 2:1872622/76‑1 (MQ=255) cAGGAAGAAGGCAATTTGCGCTTCGCTGTCTTACAGGACCCGGAAGTGAATTCGCGCTTTTATATCTACGAAGCCt < 2:1872636/76‑1 (MQ=255) ggAAGAAGGCAATTTGCGCTTCGCTGTCTTACAGGACCCGGAAGTGAATTCGCGCTTTTATATCTACGAAGCCtat < 2:1896205/76‑1 (MQ=255) aagaagGCAATTTGCGCTTCGCTGTCTTACAGGACCCGGAAGTGAATTCGCGCTTTTATATCTACGAAGCCTATaa < 1:1838305/76‑1 (MQ=255) agaagGCAATTTGCGCTTCGATGTCTTACAGGACCCGGAAGTGAATTCGCGCTTTTATATCTACGAAGCCTATaaa < 2:1744578/76‑1 (MQ=255) gCAATTTGCGCTTCGCTGTCTTACAGGACCCGGAAGTGAATTCGCGCTTTTATATCTACGAAGCCTATAAAGATGa < 1:853704/76‑1 (MQ=255) cAATTTGCGCTTCGCTGTCTTACAGGACCCGGAAGTGAATTCGCGCTTTTATATCTACGAAGCCTATAAAGATGaa < 2:139511/76‑1 (MQ=255) tGCGCTTCGCTGTCTTACAGGACCCGGAAGTGAATTCGCGCTTTTATATCTACGAAGCCTATAAAGATGAAGACGc > 1:303618/1‑76 (MQ=255) cgcTTCGATGTCTTACAGGACCCGGAAGTGAATTCGCGCTTTTATATCTACGAAGCCTATAAAGATGAAGACGCAg > 1:1947284/1‑76 (MQ=255) gcTTCGCTGTCTTACAGGACCCGGAAGTGAATTCGCGCTTTTATATCTACGAAGCCTATAAAGATGAAGACGCAGt > 2:1281973/1‑76 (MQ=255) ttCGCTGTCTTACAGGACCCGGAAGTGAATTCGCGCTTTTATATCTACGAAGCCTATAAAGATGAAGACGCAGTgg < 2:1302492/76‑1 (MQ=255) tCGCTGTCTTACAGGACCCGGAAGTGAATTCGCGCTTTTATATCTACGAAGCCTATAAAGATGAAGACGCAGTGGc < 2:1137444/76‑1 (MQ=255) | GTTGACGAGTTTATCGAAGTTTTTCGCCAGAACCACCTGGGCTCTGTACAGGAAGAAGGCAATTTGCGCTTCGATGTCTTACAGGACCCGGAAGTGAATTCGCGCTTTTATATCTACGAAGCCTATAAAGATGAAGACGCAGTGGC > REL606/1573586‑1573731 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |