| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 1,588,754 | A→C | F50L (TTT→TTG) | eamA ← | cysteine and O‑acetyl‑L‑serine efflux system |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 1,588,754 | 0 | A | C | 97.0% | 261.8 / ‑1.9 | 67 | F50L (TTT→TTG) | eamA | cysteine and O‑acetyl‑L‑serine efflux system |
| Reads supporting (aligned to +/- strand): ref base A (1/1); new base C (31/34); total (32/35) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.63e-01 | |||||||||||
AAGCAAACTGCGCAAAACTGATGGTTAATCCATACCCCAGCAGCAAATTCAGTGGTACTTTCGGTCGTGCGACAAAAAAGATAGCCGGAAAAGCGACCAACATAAAGCGCAAACCGGCCAGCATTAGCGGTGGCATGTTATGAAGC > REL606/1588681‑1588826 | aagCAAACTGCGCAAAACTGATGGTTAATCCATACCCCAGCAGCAAATTCAGTGGTACTTTCGGTCGTGCGACcaa > 2:213602/1‑76 (MQ=255) agCAAACTGCGCAAAACTGATGGTTAATCCATACCCCAGCAGCAAATTCAGTGGTACTTTCGGTCGTGCGACcaaa < 1:263070/76‑1 (MQ=255) gCAAACTGCGCAAAACTGATGGTTAATCCATACCCCAGCAGCAAATTCAGTGGTACTTTCGGTCGTGCGACcaaaa < 2:892673/76‑1 (MQ=255) cAAACTGCGCAAAACTGATGGTTAATCCATACCCCAGCAGCAAATTCAGTGGTACTTTCGGTCGTGCGACcaaaaa > 1:472902/1‑76 (MQ=255) aaaCTGCGCAAAACTGATGGTTAATCCATACCCCAGCAGCAAATTCAGTGGTACTTTCGGTCGTGCGACCAAAAAg > 2:170602/1‑76 (MQ=255) aaCTGCGCAAAACTGATGGTTAATCCATACCCCAGCAGCAAATTCAGTGGTACTTTCGGTCGTGCGACCAAAAAGa > 1:359544/1‑76 (MQ=255) aCTGCGCAAAACTGATGGTTAATCCATACCCCGGCAGCAAATTCAGTGGTACTTTCGGTCGTGCGACCAAAAAGAt < 2:239372/76‑1 (MQ=255) aCTGCGCAAAACTGATGGTTAATCCATACCCCAGCAGCAAATTCAGTGGTACTTTCGGTCGTGCGACCAAAAAGAt < 1:1548183/76‑1 (MQ=255) aCTGCGCAAAACTGATGGTTAATCCATACCCCAGCAGCAAATTCAGTGGTACTTTCGGTCGTGCGACCAAAAAGAt < 2:836925/76‑1 (MQ=255) cTGCGCAAAACTGATGGTTAATCCATACCCCAGCAGCAAATTCAGTGGTACTTTCGGTCGTGCGACAAAAAAGATa > 2:20865/1‑76 (MQ=255) tGCGCAAAACTGATGGTTAATCCATACCCCAGCAGCAAATTCAGTGGTACTTTCGGTCGTGCGACCAAAAAGATAg < 2:471717/76‑1 (MQ=255) tGCGCAAAACTGATGGTTAATCCATACCCCAGCAGCAAATTCAGTGGTACTTTCGGTCGTGCGACCAAAAAGATAg < 2:1786090/76‑1 (MQ=255) gcgcAAAACTGATGGTTAATCCATACCCCAGCAGCAAATTCAGTGGTACTTTCGGTCGTGCGACCAAAAAGATAGc < 1:119574/76‑1 (MQ=255) aaaaCTGATGGTTAATCCATACCCCAGCAGCAAATTCAGTGGTACTTTCGGTCGTGCGACCAAAAAGATAGCCGGa > 1:937818/1‑76 (MQ=255) aaaaCTGATGGTTAATCCATACCCCAGCAGCAAATTCAGTGGTACTTTCGGTCGTGCGACCAAAAAGATAGCCGGa > 1:1884089/1‑76 (MQ=255) aaaCTGATGGTTAATCCATACCCCAGCAGCAAATTCAGTGGTACTTTCGGTCGTGCGACCAAAAAGATAGCCGGaa < 2:525639/76‑1 (MQ=255) aaaCTGATGGTTAATCCATACCCCAGCAGCAAATTCAGTGGTACTTTCGGTCGTGCGACCAAAAAGATAGCCGGaa > 2:1803795/1‑76 (MQ=255) aaCTGATGGTTAATCCATACCCCAGCAGCAAATTCAGTGGTACTTTCGGTCGTGCGACCAAAAAGATAGCCGGaaa > 2:1239211/1‑76 (MQ=255) aaCTGATGGTTAATCCATACCCCAGCAGCAAATTCAGTGGTACTTTCGGTCGTGCGACCAAAAAGATAGCCGGaaa < 2:775846/76‑1 (MQ=255) cTGATGGTTAATCCATACCCCAGCAGCAAATTCAGTGGTACTTTCGGTCGTGCGACCAAAAAGATAGCCGGAAAAg > 1:450637/1‑76 (MQ=255) gATGGTTAATCCATACCCCAGCAGCAAATTCAGTGGTACTTTCGGTCGTGCGACCAAAAAGATAGCCGGAAAAGCg > 1:674986/1‑76 (MQ=255) aTGGTTAATCCATACCCCAGCAGCAAATTCAGTGGTACTTTCGGTCGTGCGACCAAAAAGATAGCCGGAAAAGCGa > 2:1338722/1‑76 (MQ=255) tGGTTAATCCATACCCCAGCAGCAAATTCAGTGGTACTTTCGGTCGTGCGACCAAAAAGATAGCCGGAAAAGCGAc < 2:783732/76‑1 (MQ=255) ggTTAATCCATACCCCAGCAGCAAATTCAGTGGTACTTTCGGTCGTGCGACCAAAAAGATAGCCGGAAAAGCGAcc > 2:1043908/1‑76 (MQ=255) ggTTAATCCATACCCCAGCAGCAAATTCAGTGGTACTTTCGGTCGTGCGACCAAAAAGATAGCCGGAAAAGCGAcc > 2:498119/1‑76 (MQ=255) gTTAATCCATACCCCAGCAGCAAATTCAGTGGTACTTTCGGTCGTGCGACCAAAAAGATAGCCGGAAAAGCGACCa < 1:466830/76‑1 (MQ=255) ttAATCCATACCCCAGCAGCAAATTCAGTGGTACTTTCGGTCGTGCGACCAAAAAGATAGCCGGAAAAGCGACCaa < 1:10820/76‑1 (MQ=255) ttAATCCATACCCCAGCAGCAAATTCAGTGGTACTTTCGGTCGTGCGACCAAAAAGATAGCCGGAAAAGCGACCaa < 2:964462/76‑1 (MQ=255) ttAATCCATACCCCAGCAGCAAATTCAGTGGTACTTTCGGTCGTGCGACCAAAAAGATAGCCGGAAAAGCGACCaa > 1:1743617/1‑76 (MQ=255) tAATCCATACCCCAGCAGCAAATTCAGTGGTACTTTCGGTCGTGCGACCAAAAAGATAGCCGGAAAAGCGACCAAc > 1:1806111/1‑76 (MQ=255) tAACCCATACCCCAGCAGCAAATTCAGTGGTACTTTCGGTCGTGCGACCAAAAAGATAGCCGGAAAAGCGACCAAc > 1:1875320/1‑76 (MQ=255) aaTCCATACCCCAGCAGCAAATTCAGTGGTACTTTCGGTCGTGCGACCAAAAAGATAGCCGGAAAAGCGACCAACa < 2:1882983/76‑1 (MQ=255) aTCCATACCCCAGCAGCAAATTCAGTGGTACTTTCGGTCGTGCGACCAAAAAGATAGCCGGAAAAGCGACCAACAt > 1:1575207/1‑76 (MQ=255) aTCCATACCCCAGCAGCAAATTCAGTGGTACTTTCGGTCGTGCGACAAAAAAGATAGCCGGAAAAGCGACCAACAt < 1:1073708/76‑1 (MQ=255) tCCATACCCCAGCAGCAAATTCAGTGGTACTTTCGGTCGTGCGACCAAAAAGATAGCCGGAAAAGCGACCAACATa > 2:822003/1‑76 (MQ=255) ccATACCCCAGCAGCAAATTCAGTGGTACTTTCGGTCGTGCGACCAAAAAGATAGCCGGAAAAGCGACCAACATaa < 2:178883/76‑1 (MQ=255) cATACCCCAGCAGCAAATTCAGTGGTACTTTCGGTCGTGCGACCAAAAAGATAGCCGGAAAAGCGACCAACATaaa > 1:1760572/1‑76 (MQ=255) cATACCCCAGCAGCAAATTCAGTGGTACTTTCGGTCGTGCGACCAAAAAGATAGCCGGAAAAGCGACCAACATaaa > 2:1547888/1‑76 (MQ=255) aTACCCCAGCAGCAAATTCAGTGGTACTTTCGGTCGTGCGACCAAAAAGATAGCCGGAAAAGCGACCAACATAAAg < 2:611709/76‑1 (MQ=255) aCCCCAGCAGCAAATTCAGTGGTACTTTCGGTCGTGCGACCAAAAAGATAGCCGGAAAAGCGACCAACATAAAgcg < 2:1905735/76‑1 (MQ=255) aCCCCAGCAGCAAATTCAGTGGTACTTTCGGTCGTGCGACCAAAAAGATAGCCGGAAAAGCGACCAACATAAAgcg > 1:1917546/1‑76 (MQ=255) cagcagCAAATTCAGTGGTACTTTCGGTCGTGCGACCAAAAAGATAGCCGGAAAAGCGACCAACATAAAGCGCaaa > 1:1468134/1‑76 (MQ=255) agcagcAAATTCAGTGGTACTTTCGGTCGTGCGACCAAAAAGATAGCCGGAAAAGCGACCAACATAAAGCGCAAAc < 2:1292711/76‑1 (MQ=255) agcagcAAATTCAGTGGTACTTTCGGTCGTGCGACCAAAAAGATAGCCGGAAAAGCGACCAACATAAAGCGCAAAc > 2:879087/1‑76 (MQ=255) gcagcaAATTCAGTGGTACTTTCGGTCGTGCGACCAAAAAGATAGCCGGAAAAGCGACCAACATAAAGCGCAAAcc > 2:1819741/1‑76 (MQ=255) cagcaAATTCAGTGGTACTTTCGGTCGTGCGACCAAAAAGATAGCCGGAAAAGCGACCAACATAAAGCGCAAACCg < 2:1397031/76‑1 (MQ=255) gcaAATTCAGTGGTACTTTCGGTCGTGCGACCAAAAAGATAGCCGGAAAAGCGACCAACATAAAGCGCAAACCgac > 2:1581324/1‑74 (MQ=255) caAATTCAGTGGTACTTTCGGTCGTGCGACCAAAAAGATAGCCGGAAAAGCGACCAACATAAAGCGCAAACCGGcc < 1:1041164/76‑1 (MQ=255) caAATTCAGTGGTACTTTCGGTCGTGCGACCAAAAAGATAGCCGGAAAAGCGACCAACATAAAGCGCAAACCGGcc < 1:1343598/76‑1 (MQ=255) caAATTCAGTGGTACTTTCGGTCGTGCGACCAAAAAGATAGCCGGAAAAGCGACCAACATAAAGCGCAAACCGGcc < 1:711718/76‑1 (MQ=255) aaTTCAGTGGTACTTTCGGTCGTGCGACCAAAAAGATAGCCGGAAAAGCGACCAACATAAAGCGCAAACCGGCCAg < 2:411182/76‑1 (MQ=255) aaTTCAGTGGTACTTTCGGTCGTGCGACCAAAAAGATAGCCGGAAAAGCGACCAACATAAAGCGCAAACCGGCCAg > 1:1042641/1‑76 (MQ=255) aaTTCAGTGGTACTTTCGGTCGTGCGACCAAAAAGATAGCCGGAAAAGCGACCAACATAAAGCGCAAACCGGCCAg < 1:1337580/76‑1 (MQ=255) aTTCAGTGGTACTTTCGGTCGTGCGACCAAAAAGATAGCCGGAAAAGCGACCAACATAAAGCGCAAACCGGCCAGc > 1:693426/1‑76 (MQ=255) aGTGGTACTTTCGGTCGTGCGACCAAAAAGATAGCCGGAAAAGCGACCAACATAAAGCGCAAACCGGCCAGCATTa < 2:488638/76‑1 (MQ=255) tGGTACTTTCGGTCGTGCGACCAAAAAGATAGCCGGAAAAGCGACCAACATAAAGCGCAAACCGGCCAGCATTAGc > 1:1291932/1‑76 (MQ=255) tGGTACTTTCGGTCGTGCGACCAAAAAGATAGCCGGAAAAGCGACCAACATAAAGCGCAAACCGGCCAGCATTAGc > 1:1329446/1‑76 (MQ=255) tACTTTCGGTCGTGCGACCAAAAAGATAGCCGGAAAAGCGACCAACATAAAGCGCAAACCGGCCAGCATTAGCggg > 2:386309/1‑75 (MQ=255) tACTTTCGGTCGTGCGACCAAAAAGATAGCCGGAAAAGCGACCAACATAAAGCGCAAACCGGCCAGCATTAGCGGt < 1:622647/76‑1 (MQ=255) ttCGGTCGTGCGACCAAAAAGATAGCCGGAAAAGCGACCAACATAAAGCGCAAACCGGCCAGCATTAGCGGTGGCa > 2:548001/1‑76 (MQ=255) ttCGGTCGTGCGACCAAAAAGATAGCCGGAAAAGCGACCAACATAAAGCGCAAACCGGCCAGCATTAGCGGTGGCa > 2:1942009/1‑76 (MQ=255) ggTCGTGCGACCAAAAAGATAGCCGGAAAAGCGACCAACATAAAGCGCAAACCGGCCAGCATTAGCGGTGGCATGt < 1:950541/76‑1 (MQ=255) tCGTGCGACCAAAAAGATAGCCGGAAAAGCGACCAACATAAAGCGCAAACCGGCCAGCATTAGCGGTGGCATGTTa < 2:1036576/76‑1 (MQ=255) tCGTGCGACCAAAAAGATAGCCGGAAAAGCGACCAACATAAAGCGCAAACCGGCCAGCATTAGCGGTGGCATGTTa < 2:1254282/76‑1 (MQ=255) tCGTGCGACCAAAAAGATAGCCGGAAAAGCGACCAACATAAAGCGCAAACCGGCCAGCATTAGCGGTGGCATGTTa < 1:553643/76‑1 (MQ=255) cGTGCGACCAAAAAGATAGCCGGAAAAGCGACCAACATAAAGCGCAAACCGGCCAGCATTAGCGGTGGCATGTTAt < 2:1016287/76‑1 (MQ=255) gTGCGACCAAAAAGATAGCCGGAAAAGCGACCAACATAAAGCGCAAACCGGCCAGCATTAGCGGTGGCATGTTATg < 2:278937/76‑1 (MQ=255) tGCGACCAAAAAGATAGCCGGAAAAGCGACCAACATAAAGCGCAAACCGGCCAGCATTAGCGGTGGCATGTTATGa > 2:1098788/1‑76 (MQ=255) tGCGACCAAAAAGATAGCCGGAAAAGCGACCAACATAAAGCGCAAACCGGCCAGCATTAGCGGTGGCATGTTATGa < 2:1340147/76‑1 (MQ=255) gCGACCAAAAAGATAGCCGGAAAAGCGACCAACATAAAGCGCAAACCGGCCAGCATTAGCGGTGGCATGTTATGaa < 1:1687428/76‑1 (MQ=255) cGACCAAAAAGATAGCCGGAAAAGCGACCAACATAAAGCGCAAACCGGCCAGCATTAGCGGTGGCATGTTATGAAg < 2:1968392/76‑1 (MQ=255) gACCAAAAAGATAGCCGGAAAAGCGACCAACATAAAGCGCAAACCGGCCAGCATTAGCGGTGGCATGTTATGAAGc < 2:832729/76‑1 (MQ=255) | AAGCAAACTGCGCAAAACTGATGGTTAATCCATACCCCAGCAGCAAATTCAGTGGTACTTTCGGTCGTGCGACAAAAAAGATAGCCGGAAAAGCGACCAACATAAAGCGCAAACCGGCCAGCATTAGCGGTGGCATGTTATGAAGC > REL606/1588681‑1588826 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |