Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC	REL606	474,296	Δ11 bp	coding (1000‑1010/1677 nt)	ybaL ←	predicted transporter with NAD(P)‑binding Rossmann‑fold domain

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	REL606	= 474295	1 (0.020)	46 (0.840)	41/150	0.3	97.9%	coding (1011/1677 nt)	ybaL	predicted transporter with NAD(P)‑binding Rossmann‑fold domain
	?	REL606	474307 =	1 (0.020)					coding (999/1677 nt)	ybaL	predicted transporter with NAD(P)‑binding Rossmann‑fold domain

GCAGTAAATTCAATGCCATTCCCAGTCCCGCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  REL606/474222‑474295 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑CGTACGTTGGGAGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAG  >  REL606/474307‑474381                                                                                                                                                       GCAGTAAATTCAATGCCATTCCCAGTCCCGCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGCG                                                                           <  2:880889/76‑1  CAGTAAATTCAATGCCATTCCCAGTCCCGCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGCGT                                                                          >  1:18390/1‑76   AGTAAATTCAATGCCATTCCCAGTCCCGCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGCGTA                                                                         <  2:645291/76‑1    GTAAATTCAATGCCATTCCCAGTCCCGCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGCGTAC                                                                        <  1:1441814/76‑1    GTAAATTCAATGCCATTCCCAGTCCCGCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGCGTAC                                                                        <  2:1684040/76‑1      AAATTCAATGCCATTCCCAGTCCCGCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGCGTACGT                                                                      >  2:97605/1‑76      AAATTCAATGCCATTCCCAGTCCCGCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGCGTACGT                                                                      <  2:680392/76‑1       AATTCAATGCCATCCCCAGTCCCGCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGCGTACGTT                                                                     <  2:280351/76‑1        ATTCAATGCCATTCCCAGTCCCGCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGCGTACGTTG                                                                    <  2:1578540/76‑1         TTCAATGCCATTCCCAGTCCCGCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGCGTACGTTGG                                                                   <  1:417504/76‑1           CAATGCCATTCCCAGTCCCGCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGCGTACGTTGGGA                                                                 <  2:939970/76‑1               GCCATTCCCAGTCCCGCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGCGTACGTTGGGAGTGA                                                             <  1:340004/76‑1                  ATTCCCAGTCCCGCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGCGTACGTTGGGAGTGACCA                                                          >  2:494445/1‑76                   TTCCCAGTCCCGCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGCGTACGTTGGGAGTGACCAA                                                         >  1:1112097/1‑76                    TCCCAGTCCCGCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGCGTACGTTGGGAGTGACCAAA                                                        >  1:64401/1‑76                     CCCAGTCCCGCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGCGTACGTTGGGAGTGACCAAAC                                                       <  2:869300/76‑1                     CCCAGTCCCGCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGCGTACGTTGGGAGTGACCAAAC                                                       >  1:1252728/1‑76                       CAGTCCCGCCAGGATAAACGCGAACTCACCAATCTTCGCCAGTCTGGCGGCGCGTACGTTGAGAGTGACCAAACAG                                                     >  2:979314/1‑76                       CAGTCCCGCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGCGTACGTTGGGAGTGACCAAACAG                                                     <  1:200169/76‑1                         GTCCCGCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGCGTACGTTGGGAGTGACCAAACAGTC                                                   <  1:1369286/76‑1                             CGCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGCGTACGTTGGGAGTGACCAAACAGTCGCAC                                               <  2:537034/76‑1                                CAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGCGTACGTTGGGAGTGACCAAACAGTCGCACCAG                                            >  2:397766/1‑76                                 AGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGCGTACGTTGGGAGTGACCAAACAGTCGCACCAGG                                           >  1:1666378/1‑76                                    ATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGCGTACGTTGGGAGTGACCAAACAGTCGCACCAGGAAA                                        >  1:1735207/1‑76                                    ATAAACGCGAACTCACCAATCTGCGCCAGGCTCGCGGCGCGTACGTTGGGAGTGACCAAACAGTCGCACCAGGAAA                                        >  2:824748/1‑76                                      AAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGCGTACGTTGGGAGTGACCAAACAGTCGCACCAGGAAAAA                                      >  1:1670792/1‑76                                      AAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGCGTACGTTGGGAGTGACCAAACAGTCGCACCAGGAAAAA                                      >  2:313061/1‑76                                       AACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCGCGTACGTTGGGAGTGACCAAACAGTCGCACCAGGAAAAAT                                     >  1:810413/1‑76                                               CTCACCAATCTGCGCCAGGCTGGCGGCGCGTACGTTGGGAGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAA                             >  2:1501794/1‑76                                               CTCACCAATCTGCGCCAGGCTGGCGGCGCGGACGTTGGGAGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAA                             >  1:1525091/1‑76                                               CTCACCAATCTGCGCCAGGCGGGCGGCGCGTACGTTGGGAGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAA                             <  2:961171/76‑1                                                TCACCAATCTGCGCCAGGCTGGCGGCGCGTACGTGGGGAGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAAC                            >  2:1140222/1‑76                                                 CACCAATCTGCGCCAGGCTGGCGGCGCGTACGTTGGGAGTGACCAAACAGTCGCACCAGGGAAAATGCGGCTAACG                           >  1:1537630/1‑76                                                 CACCAATCTGCGCCAGGCTGGCGGCGCGTACGTTGGGAGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACG                           <  1:938730/76‑1                                                 CACCAATCTGCGCCAGGCTGGCGGCGCGTACGTTGGGAGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACG                           <  2:619632/76‑1                                                   CCAATCTGCGCCAGGCTGGCGGCGCGTACGTTGGGAGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGAC                         <  1:1540100/76‑1                                                    CAATCTGCGCCAGGCTGGCGGCGCGTACGTTGGGAGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGACT                        >  1:143888/1‑76                                                      ATCTGCGCCAGGCTGGCGGCGCGTACGTTGGGAGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGACTTA                      <  2:305816/76‑1                                                        CTGCGCCAGGCTGGCGGCGCGTACGTTGGGAGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGACTTACC                    >  1:637750/1‑76                                                         TGCGCCAGGCTGGCGGCGCGTACGTTGGGAGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGACTTACCA                   >  2:562864/1‑76                                                              CAGGCTGGCGGCGCGTACGTTGGGAGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAAAGCCTTACCAAAGAG              >  2:1460518/1‑76                                                                  CTGGCGGCGCGTACGTTGGGAGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATA          <  2:1505066/76‑1                                                                    GGCGGCGCGTACGTTGGGAGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAAT        >  2:847910/1‑76                                                                    GGCGGCGCGTACGTTGGGAGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAAT        <  2:1340697/76‑1                                                                      CGGCGCGTACGTTGGGAGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCG      <  1:642188/76‑1                                                                          GCGTACGTTGGGAGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAG  >  1:1314541/1‑76                                                                                                                                                       GCAGTAAATTCAATGCCATTCCCAGTCCCGCCAGGATAAACGCGAACTCACCAATCTGCGCCAGGCTGGCGGCG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  REL606/474222‑474295 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑CGTACGTTGGGAGTGACCAAACAGTCGCACCAGGAAAAATGCGGCTAACGACTTACCAAACAGAATAATCGCCAG  >  REL606/474307‑474381

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.