Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	3,477,922	A→C	V250G (GTC→GGC)	malQ ←	4‑alpha‑glucanotransferase (amylomaltase)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	3,477,922	0	A	C	100.0%	189.8 / NA	46	V250G (GTC→GGC)	malQ	4‑alpha‑glucanotransferase (amylomaltase)
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (30/16);  total (30/16)

TCACGTTGTGCGAAACCTTTCCACGCCATTCGTAATGCTGTCATTTTTAGGGCGGTAACCGTGGAGTAATCGACCCAGTCGGCATCCCGCGCCTGTTGCAGCGTCTGTTGCGTGGTCGGCAACTGCCACCAGGCCTGAGCCTCTT  >  REL606/3477850‑3477994                                                                         |                                                                         tcaCGTTGTGCGAAACCTTTCCACGCCATTCGTAATGCTGTCATTTTTAGGGCGGTAACCGTGGAGTAATCGcccc                                                                       >  2:1099358/1‑76 (MQ=255)  caCGTTGTGCGAAACCTTTCCACGCCATTCGTAATGCTGTCATTTTTAGGGCGGTAACCGTGGAGTAATCGCCCCa                                                                      >  2:607334/1‑76 (MQ=255)         tgCGAAACCTTTCCACGCCATTCGTAATGCTGTCATTTTTAGGGCGGTAACCGTGGAGTAATCGCCCCAGTCGGCa                                                               >  1:1054252/1‑76 (MQ=255)              aaCCTTTCCACGCCATTCGTAATGCTGTCATTTTTAGGGCGGTAACCGTGGAGTAATCGCCCCAGTCGGCATCCcg                                                          <  2:515070/76‑1 (MQ=255)              aaCCTTTCCACGCCATTCGTAATGCTGTCATTTTTAGGGCGGTAACCGTGGAGTAATCGCCCCAGTCGGCATCCcg                                                          <  1:1463402/76‑1 (MQ=255)                   ttCCACGCCATTCGTAATGCTGTCATTTTTAGGGCGGTAACCGTGGAGTAATCGCCCCAGTCGGCATCCCGCGCCt                                                     <  1:1086713/76‑1 (MQ=255)                   ttCCACGCCATTCGTAATGCTGTCATTTTTAGGGCGGTAACCGTGGAGTAATCGCCCCAGTCGGCATCCCGCGCCt                                                     <  2:863663/76‑1 (MQ=255)                   ttCCACGCCATTCGTAATGCTGTCATTTTTAGGGCGGTAACCGTGGAGTAATCGCCCCAGTCGGCATCCCGCGCCt                                                     >  1:1259243/1‑76 (MQ=255)                   ttCCACGCCATTCGTAATGCTGTCATTTTTAGGGCGGTAACCGTGGAGTAATCGCCCCAGTCGGCATCCCGCGCCt                                                     >  2:791625/1‑76 (MQ=255)                   ttCCACGCCATTCGTAATGCTGTCATTTTTAGGGCGGTAACCGTGGAGTAATCGCCCCAGTCGGCATCCCGCGCCt                                                     <  1:1371337/76‑1 (MQ=255)                   ttCCACCCCATTCGTAATGCTGTCATTTTTAGGGCGGTAACCGTGGAGTAATCGCCCCAGTCGGCATCCCGCGCCt                                                     <  1:375650/76‑1 (MQ=255)                     ccACGCCATTCGTAATGCTGTCATTTTTAGGGCGGTAACCGTGGAGTAATCGCCCCAGTCGGCATCCCGCGCCTGt                                                   >  1:1437332/1‑76 (MQ=255)                      cACGCCATTCGTAATGCTGTCATTTTTAGGGCGGTAACCGTGGAGTAATCGCCCCAGTCGGCATCCCGCGCCTGtt                                                  >  1:658038/1‑76 (MQ=255)                       aCGCCATTCGTAATGCTGTCATTTTTAGGGCGGTAACCGTGGAGTAATCGCCCCAGTCGGCATCCCGCGCCTGTTg                                                 >  1:1482840/1‑76 (MQ=255)                       aCGCCATTCGTAATGCTGTCATTTTTAGGGCGGTAACCGTGGAGTAATCGCCCCAGTCGGCATCCCGCGCCTGTTg                                                 >  1:1413736/1‑76 (MQ=255)                        cGCCATTCGTAATGCTGTCATTTTTAGGGCGGTAACCGTGGAGTAATCGCCCCAGTCGGCATCCCGCGCCTGTTGc                                                >  1:1644717/1‑76 (MQ=255)                        cGCCATTCGTAATGCTGTCATTTTTAGGGCGGTAACCGTGGAGTAATCGCCCCAGTCGGCATCCCGCGCCTGTTGc                                                >  1:1126636/1‑76 (MQ=255)                            aTTCGTAATGCTGTCATTTTTAGGGCGGTAACCGTGGAGTAATCGCCCCAGTCGGCATCCCGCGCCTGTTGCAGCg                                            <  2:779489/76‑1 (MQ=255)                               cGTAATGCTGTCATTTTTAGGGCGGTAACCGTGGAGTAATCGCCCCAGTCGGCATCCCGCGCCTGTTGCAGCGTCt                                         <  2:1201072/76‑1 (MQ=255)                               cGTAATGCTGTCATTTTTAGGGCGCTAACCGTGGAGTAATCGCCCCAGTCGGCATCCCGCGCCTGTTGCAGCGTCt                                         >  2:284076/1‑76 (MQ=255)                                 tAATGCTGTCATTTTTAGGGCGGTAACCGTGGAGTAATCGCCCCAGTCGGCATCCCGCGCCTGTTGCAGCGTCTGt                                       >  2:567100/1‑76 (MQ=255)                                  aaTGCTGTCATTTTTAGGGCGGTAACCGTGGAGTAATCGCCCCAGTCGGCATCCCGCGCCTGTTGCAGCGTCTGtt                                      >  2:1091989/1‑76 (MQ=255)                                  aaTGCTGTCATTTTTAGGGCGGTAACCGTGGAGTAATCGCCCCAGTCGGCATCCCCCGCATGTTGCAGCGTCTGtt                                      >  2:1094418/1‑76 (MQ=255)                                    tGCTGTCATTTTTAGGGCGGTAACCGTGGAGTAATCGCCCCAGTCGGCATCCCGCGCCTGTTGCAGCGTCTGTTGc                                    >  1:1302873/1‑76 (MQ=255)                                      cTGTCATTTTTAGGGCGGTAACCGTGGAGTAATCGCCCCAGTCGGCATCCCGCGCCTGTTGCAGCGTCTGTTGCgc                                  >  1:1295324/1‑75 (MQ=255)                                          cATTTTTAGGGCGGTAACCGTGGAGTAATCGCCCCAGTCGGCATCCCGCGCCTGTTGCAGCGTCTGTTGCGTgggc                              >  1:760649/1‑74 (MQ=255)                                             ttttAGGGCGGTAACCGTGGAGTAATCGCCCCAGTCGGCATCCCGCGCCTGTTGCAGCGTCTGTTGCGTGGTCGGc                           >  1:636528/1‑76 (MQ=255)                                                tAGGGCGGTAACCGTGGAGTAATCGCCCCAGTCGGCATCCCGCGCCTGTTGCAGCGTCTGTTGCGTGGTCGGCAAc                        >  2:917991/1‑76 (MQ=255)                                                tAGGGCGGTAACCGTGGAGTAATCGCCCCAGTCGGCATCCCGCGCCTGTTGCAGCGTCTGTTGCGTGGTCGGCAAc                        <  2:1229987/76‑1 (MQ=255)                                                tAGGGCGGTAACCGTGGAGTAATCGCCCCAGTCGGCATCCCGCGCCTGTTGCAGCGTCTGTTGCGTGGTCGGCAAc                        >  2:125305/1‑76 (MQ=255)                                                tAGGGCGGTAACCGTGGAGTAATCGCCCCAGTCGGCATCCCGCGCCTGTTGCAGCGTCTGTTGCGTGGCCGGCAAc                        >  2:857270/1‑76 (MQ=255)                                                   ggCGGTAACCGTGGAGTAATCGCCCCAGTCGGCATCCCGCGCCTGTTGCAGCGTCTGTTGCGTGGTCGGCAACTGc                     >  1:246555/1‑76 (MQ=255)                                                      ggTAACCGTGGAGTAATCGCCCCAGTCGGCATCCCGCGCCTGTTGCAGCGTCTGTTGCGTGGTCGGCAACTGccac                  >  1:655693/1‑76 (MQ=255)                                                      ggTAACCGTGGAGTAATCGCCCCAGTCGGCATCCCGCGCCTGTTGCAGCGTCTGTTGCGTGGTCGGCAACTGccac                  <  2:746496/76‑1 (MQ=255)                                                         aaCCGTGGAGTAATCGCCCCAGTCGGCATCCCGCGCCTGTTGCAGCGTCTGTTGCGTGGTCGGCAACTGCCACCAg               >  1:1452612/1‑76 (MQ=255)                                                            cGTGGAGTAATCGCCCCAGTCGGCATCCCGCGCCTGTTGCAGCGTCTGTTGCGTGGTCGGCAACTGCCACCAGGcc            >  1:1572280/1‑76 (MQ=255)                                                              tGGCGTAATAGCCCCAGCCGGCATCCCGCGCCTGTTGCAGCGTCTGTTGCGTGGTCGGCAACTGCCACCAGGCCTg          <  2:169417/76‑1 (MQ=255)                                                              tGGAGTAATCGCCCCAGTCGGCATCCCGCGCCTGTTGCAGCGTCTGTTGCGTGGTCGGCAACTGcca                   >  2:836161/1‑67 (MQ=255)                                                              tGGAGTAATCGCCCCAGTCGGCATCCCGCGCCTGTTGCAGCGTCTGTTGCGTGGTCGGCAACTGcca                   <  1:836161/67‑1 (MQ=255)                                                              tGGAGTAATCGCCCCAGTCGGCATCCCGCGCCTGTTGCAGCGTCTGTTGCGTGGTCGGCAACTGCCACCAGGCCTg          <  2:658038/76‑1 (MQ=255)                                                              tGGAGTAATCGCCCCAGTCGGCATCCCGCGCCTGTTGCAGCGTCTGTTGCGTGGTCGGCAACTGCCACCAGGCCTg          >  2:54354/1‑76 (MQ=255)                                                              tGGAGTAATCGCCCCAGTCGGCATCCCGCGCCTGTTGCAGCGTCTGTTGCGTGGTCGGCAACTGCCACCAGGCCTg          <  2:412345/76‑1 (MQ=255)                                                              tGGAGTAATCGCCCCAGTCGGCATCCCGCGCCTGTTGCAGCGTCTGTTGCGTGGTCGGCAACTGCCACCAGGCCTg          >  2:1332948/1‑76 (MQ=255)                                                               ggAGTAATCGCCCCAGTCGGCATCCCGCGCCTGTTGCAGCGTCTGTTGCGTGGTCGGCAACTGCCACCAGGCCTGa         <  2:668072/76‑1 (MQ=255)                                                                gAGTAATCGCCCCAGTCGGCATCCCGCGCCTGTTGCAGCGTCTGTTGCGTGGTCGGCAACTGCCACCAGGCCTGAg        <  1:1162371/76‑1 (MQ=255)                                                                    aaTCGCCCCAGTCGGCATCCCGCGCCTGTTGCAGCGTCTGTTGCGTGGTCGGCAACTGCCACCAGGCCTGAGCctc    >  2:1593091/1‑76 (MQ=255)                                                                    aaTCGCCCCAGTCGGCATCCCGCGCCTGTTGCAGCGTCTGTTGCGTGGTCGGCAACTGCCACCAGGCCTGAGCctc    >  1:453023/1‑76 (MQ=255)                                                                    aaTCGCCCCAGTCGGCATCCCGCCCCTGTTGCAGCGTCTGTTGCGTGGTCGGCAACTGCCACCAGGCCTGAGCctc    <  2:1306963/76‑1 (MQ=255)                                                                      tCGCCCCAGTCGGCATCCCGCGCCTGTTGCAGCGTCTGTTGCGTGGTCGGCAACTGCCACCAGGCCTGAGCCTCtt  <  1:1385609/76‑1 (MQ=255)                                                                         |                                                                         TCACGTTGTGCGAAACCTTTCCACGCCATTCGTAATGCTGTCATTTTTAGGGCGGTAACCGTGGAGTAATCGACCCAGTCGGCATCCCGCGCCTGTTGCAGCGTCTGTTGCGTGGTCGGCAACTGCCACCAGGCCTGAGCCTCTT  >  REL606/3477850‑3477994

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑