| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 1,290,813 | C→T | Q260* (CAG→TAG) | hnr → | response regulator of RpoS |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 1,290,813 | 0 | C | T | 97.4% | 133.7 / ‑3.1 | 38 | Q260* (CAG→TAG) | hnr | response regulator of RpoS |
| Reads supporting (aligned to +/- strand): ref base C (1/0); new base T (19/18); total (20/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.55e-01 | |||||||||||
ACGGTTGCCAGAGTTGGGCGCGTTATTGAAGCAGGTAAACCATTTACTCCGTCAGGCCAATCTGCCGGGGCAGTTTCCGCTATTAGTTGGCTATTATCATCGCGAACTGAAAAATCTCATTCTGGTTTCTGCGGGTCTGAA > REL606/1290743‑1290883 | aCGGTTGCCAGAGTTGGGCGCGTTATTGAAGCAGGTAAACCATTTACTCCGTCAGGCCAATCTGCCGGGGTAGttt < 2:426386/76‑1 (MQ=255) gTTGCCAGAGTTGGGCGCGTTATTGAAGCAGGTAAACCATTTACTCCGTCAGGCCAATCTGCCGGGGTAGTTTCCg > 2:1234565/1‑76 (MQ=255) gTTGCCAGAGTTGGGCGCGTTATTGAAGCAGGTAAACCATTTACTCCGTCAGGCCAATCTGCCGGGGTAGTTTCCg < 2:169988/76‑1 (MQ=255) ttGCCAGAGTTGGGCGCGTTATTGAAGCAGGTAAACCATTTACTCCGTCAGGCCAATCTGCCGGGGTAGTTTCCGc > 1:77002/1‑76 (MQ=255) ttGCCAGAGTTGGGCGCGTTATTGAAGCAGGTAAACCATTTACTCCGTCAGGCCAATCTGCCGGGGTAGTTTCCGc > 1:1458822/1‑76 (MQ=255) cAGAGTTGGGCGCGTTATTGAAGCAGGTAAACCATTTACTCCGTCAGGCCAATCTGCCGGGGTAGTTTCCGCTAtt > 1:15064/1‑76 (MQ=255) cAGAGTTGGGCGCGTTATTGAAGCAGGTAAACCATTTACTCCGTCAGGCCAATCTGCCGGGGTAGTTTCCGCTAtt < 2:1526976/76‑1 (MQ=255) cAGAGTGGGGCGCGTTATTGAAGCAGGTAAACCATTTACTCCGTCAGGCCAATCTGCCGGGGTAGTTTCCGCTAtt < 2:928435/76‑1 (MQ=255) gagTTGGGCGCGTTATTGAAGCAGGTAAACCATTTACTCCGTCAGGCCAATCTGCCGGGGTAGTTTCCGCTATTAg < 1:511761/76‑1 (MQ=255) ttGGGCGCGTTATTGAAGCAGGTAAACCATTTACTCCGTCAGGCCAATCTGCCGGGGCAGTTTCCGCTATTAGTTg > 1:1569741/1‑76 (MQ=255) gggCGCGTTATTGAAGCAGGTAAACCATTTACTCCGTCAGGCCAATCTGCCGGGGTAGTTTCCGCTATTAGTTGGc > 2:1391534/1‑76 (MQ=255) ggCGCGTTATTGAAGCAGGTAAACCATTTACTCCGTCAGGCCAATCTGCCGGGGTAGTTTCCGCTATTAGTTGGca < 1:154399/76‑2 (MQ=255) ggCGCGTTATTGAAGCAGGTAAACCATTTACTCCGTCAGGCCAATCTGCCGGGGTAGTTTCCGCTATTAGTTGGCt < 1:1577675/76‑1 (MQ=255) ggCGCGTTATTGAAGCAGGTAAACCATTTACTCCGTCAGGCCAATCTGCCGGGGTAGTTTCCGCTATTAGTTGGCt < 2:738285/76‑1 (MQ=255) ttATTGAAGCAGGTAAACCATTTACTCCGTCAGGCCAATCTGCCGGGGTAGTTTCCGCTATTAGTTGGCTATTatc > 2:1430406/1‑76 (MQ=255) ttGAAGCAGGTAAACCATTTACTCCGTCAGGCCAATCTGCCGGGGTAGTTTCCGCTATTAGTTGGCTATTatcatc < 2:1592333/76‑1 (MQ=255) tGAAGCAGGTAAACCATTTACTCCGTCAGGCCAATCTGCCGGGGTAGTTTCCGCTATTAGTTGGCTATTATCATcg < 2:1176225/76‑1 (MQ=255) aaGCAGGAAAACCATTTACCCCGTCAGTCAAACCGCCCGGGCTATTTTCCGCTCTTAGTTGGCTATTATCATcgcg > 2:1048998/1‑76 (MQ=255) aaaCCATTTACTCCGTCAGGCCAATCTGCCGGGGTAGTTTCCGCTATTAGTTGGCTATTATCATCGCGAACTGaaa > 1:1218451/1‑76 (MQ=255) aaaCCATTTACTCCGTCAGGCCAATCTGCCGGGGTAGTTTCCGCTATTAGTTGGCTATTATCATCGCGAACTGaaa > 2:838320/1‑76 (MQ=255) aCCATTTACTCCGTCAGGCCAATCTGCCGGGGTAGTTTCCGCTATTAGTTGGCTATTATCATCGCGAACTGaaaaa < 2:1636552/76‑1 (MQ=255) aCCATTTACTCCGTCAGGCCAATCTGCCGGGGTAGTTTCCGCTATTAGTTGGCTATTATCATCGCGAACTGaaaaa < 2:153157/76‑1 (MQ=255) cATTTACTCCGTCAGGCCAATCTGCCGGGGTAGTTTCCGCTATTAGTTGGCTATTATCATCGCGAACTGAAAAAtc > 2:1330932/1‑76 (MQ=255) ttACTCCGTCAGGCCAATCTGCCGGGGTAGTTTCCGCTATTAGTTGGCTATTATCATCGCGAACTGAAAAATCTCa > 1:821679/1‑76 (MQ=255) ttACTCCGTCAGGCCAATCTGCCGGGGTAGTTTCCGCTATTAGTTGGCTATTATCATCGCGAACTGAAAAATCTCa > 1:15228/1‑76 (MQ=255) tACTCCGTCAGGCCAATCTGCCGGGGTAGTTTCCGCTATTAGTTGGCTATTATCATCGCGAACTGAAAAATCTCAt > 1:392221/1‑76 (MQ=255) aCTCCGTCAGGCCAATCTGCCGGGGTAGTTTCCGCTATTAGTTGGCTATTATCATCGCGAACTGAAAAATCTCAtt < 2:497804/76‑1 (MQ=255) tCCGTCAGGCCAATCTGCCGGGGTAGTTTCCGCTATTAGTTGGCTATTATCATCGCGAACTGAAAAATCTCATTCt < 1:1653674/76‑1 (MQ=255) tCCGTCAGGCCAATCTGCCGGGGTAGTTTCCGCTATTAGTTGGCTATTATCATCGCGAACTGAAAAATCTCATTCt > 1:233285/1‑76 (MQ=255) ccGTCAGGCCAATCTGCCGGGGTAGTTTCCGCTATTAGTTGGCTATTATCATCGCGAACTGAAAAATCTCATTCTg > 1:746762/1‑76 (MQ=255) ccGTCAGGCCAATCTGCCGGGGTAGTTTCCGCTATTAGTTGGCTATTATCATCGCGAACTGAAAAATCTCATTCTg > 1:1114995/1‑76 (MQ=255) gTCAGGCCAATCTGCCGGGGTAGTTTCCGCTATTGGTTGGCTATTATCATCGCGAACTGAAAAATCTCATTCTggn < 1:1279494/76‑2 (MQ=255) gTCAGGCCAATCTGCCGGGGTAGTTTCCGCTATTAGTTGGCTATTATCATCGCGAACTGAAAAATCTCATTCTGGt < 2:464006/76‑1 (MQ=255) tCAGGCCAATCTGCCGGGGTAGTTTCCGCTATTAGTTGGCTATTATCATCGCGAACTGAAAAATCTCATTCTGGtt > 1:1248383/1‑76 (MQ=255) ccAATCTGCCGGGGTAGTTTCCGCTATTAGTTGGCTATTATCATCGCGAACTGAAAAATCTCATTCTGGTTTCTGc < 1:448452/76‑1 (MQ=255) cTGCCGGGGTAGTTTCCGCTATTAGTTGGCTATTATCATCGCGAACTGAAAAATCTCATTCTGGTTTCTGCGGGTc > 1:1227136/1‑76 (MQ=255) gCCGGGGTAGTTTCCGCTATTAGTTGGCTATTATCATCGCGAACTGAAAAATCTCATTCTGGTTTCTGCGGGTCTg < 2:1381145/76‑1 (MQ=255) cGGGGTAGTTTCCGCTATTAGTTGGCTATTATCATCGCGAACTGAAAAATCTCATTCTGGTTTCTGCGGGTCTGaa > 1:917272/1‑76 (MQ=255) cGGGGTAGTTTCCGCTATTAGTTGGCTATTATCATCGCGAACTGAAAAATCTCATTCTGGTTTCTGCGGGTCTGaa > 2:440204/1‑76 (MQ=255) | ACGGTTGCCAGAGTTGGGCGCGTTATTGAAGCAGGTAAACCATTTACTCCGTCAGGCCAATCTGCCGGGGCAGTTTCCGCTATTAGTTGGCTATTATCATCGCGAACTGAAAAATCTCATTCTGGTTTCTGCGGGTCTGAA > REL606/1290743‑1290883 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |