| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | REL606 | 3,028,245 | Δ1 bp | coding (217/777 nt) | ECB_02828 ← | Polysialic acid transport protein kpsM |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | REL606 | 3,028,243 | 0 | G | . | 93.1% | 232.8 / 18.0 | 61 | coding (219/777 nt) | ECB_02828 | Polysialic acid transport protein kpsM |
| Reads supporting (aligned to +/- strand): ref base G (1/2); major base . (25/29); minor base A (4/0); total (30/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.12e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.99e-01 | |||||||||||
CGATAATTAAACAACCCCTGATTCGCTTCAATAGCACCTACAGAACGATTGCTGATACTGCTAAAGATAAAAAAGGGAATCAGGCCATTAAGTAAAAACACCGGGAATGAGATGTCTGGCATCGTGCGGTGCATAATGTAACCAAA > REL606/3028169‑3028314 | cGATAATTAAACAACCCCTGATTCGCTTCAATAGCACCTACAGAACGATTGCTGATACTGCTAAAGATAAAAAAgg > 1:1120431/1‑76 (MQ=255)cGATAATTAAACAACCCCTGATTCGCTTCAATAGCACCTACAGAACGATTGCTGATACTGCTAAAGATAAAAAAgg > 1:112766/1‑76 (MQ=255) aTAATTAAACAACCCCTGATTCGCTTCAATAGCACCTACAGAACGATTGCTGATACTGCTAAAGATAAAAAAggaa > 1:1315365/1‑74 (MQ=255) aTTAAACAACCCCTGATTCGCTTCAATAGCACCTACAGAACGATTGCTGATACTGCTAAAGATAAAAAAGGGAATc < 1:199607/76‑1 (MQ=255) aTTAAACAACCCCTGATTCGCTTCAATAGCACCTACAGAACGATTGCTGATACTGCTAAAGATAAAAAA‑GGAATCa < 1:1196315/76‑1 (MQ=255) ttAAACAACCCCTGATTCGCTTCAATAGCACCTACAGAACGATTGCTGATACTGCTAAAGATAAAAAA‑GGAATCAg > 1:237962/1‑76 (MQ=255) ttAAACAACCCCTGATTCGCTTCAATAGCACCTACAGAACGATTGCTGATACTGCTAAAGATAAAAAA‑GGAATCAg > 1:268822/1‑76 (MQ=255) ttAAACAACCCCTGATTCGCTTCAATAGCACCTACAGAACGATTGCTGATACTGCTAAAGATAAAAAA‑GGAATCAg > 1:945075/1‑76 (MQ=255) aaaCAACCCCTGATTCGCTTCAATAGCACCTACAGAACGATTGCTGATACTGCTAAAGATAAAAAAGGGAATCAgg > 2:260425/1‑76 (MQ=255) acaacCCCTGATTCGCTTCAATAGCACCTACAGAACGATTGCTGATACTGCTAAAGATAAAAAA‑GGAATCAGGCCa < 2:1057293/76‑1 (MQ=255) caacCCCTGATTCGCTTCAATAGCACCTACAGAACGATTGCTGATACTGCTAAAGATAAAAAA‑GGAATCAGGCCAt > 2:1244119/1‑76 (MQ=255) aacCCCTGATTCGCTTCAATAGCACCTACAGAACGATTGCTGATACTGCTAAAGATAAAAAA‑GGAATCAGGCCAtt > 2:1409684/1‑76 (MQ=255) acCCCTGATTCGCTTCAATAGCACCTACAGAACGATTGCTGATACTGCTAAAGATAAAAAA‑GGAATCAGGCCATTa < 1:580464/76‑1 (MQ=255) ccccTGATTCGCTTCAATAGCACCTACAGAACGATTGCTGATACTGCTAAAGATAAAAAA‑GGAATCAGGCCATTaa < 2:1184870/76‑1 (MQ=255) ccccTGATTCGCTTCAATAGCACCTACAGAACGATTGCTGATACTGCTAAAGATAAAAAA‑GGAATCAGGCCATTaa > 2:127217/1‑76 (MQ=255) ccccTGATTCGCTTCAATAGCACCTACAGAACGATTGCTGATACTGCTAAAGATAAAAAA‑GGAATCAGGCCATTaa > 2:1406557/1‑76 (MQ=255) ccccTGATTCGCTTCAATAGCACCTACAGAACGATTGCTGATACTGCTAAAGATAAAAAA‑GGAATCAGGCCATTaa > 2:1443498/1‑76 (MQ=255) ccccTGATTCGCTTCAATAGCACCTACAGAACGATTGCTGATACTGCTAAAGATAAAAAA‑GGAATCAGGCCATTaa > 2:996889/1‑76 (MQ=255) ccccTGATTCGCTTCAATAGCACCTACAGAACGATTGCTGATACTGCTAAAGATAAAAAA‑GGAATCAGGCCATTaa < 1:1296459/76‑1 (MQ=255) ccTGATTCGCTTCAATAGCACCTACAGAACGATTGCTGATACTGCTAAAGATAAAAAA‑GGAATCAGGCCATTAAGt < 2:497249/76‑1 (MQ=255) cTGATTCGCTTCAATAGCACCTACAGAACGATTGCTGATACTGCTAAAGATAAAAAA‑GGAATCAGGCCATTAAGTa < 2:704044/76‑1 (MQ=255) gATTCGCTTCAATAGCACCTATAGAACGATTGCTGATACTGCTAAAGATAAAAAA‑GGAATCAGGCCATTAAGTaaa > 1:811991/1‑76 (MQ=255) ttCGCTTCAATAGCACCTACAGAACGATTGCTGATACTGCTAAAGATAAAAAA‑GGAATCAGGCCATTAAGTaaaaa < 1:51943/76‑1 (MQ=255) gCTTCAATAGCACCTACAGAACGATTGCTGATACTGCTAAAGATAAAAAA‑GGAATCAGGCCATTAAGTAAAAacac < 1:1086857/76‑1 (MQ=255) ttCAATAGCACCTACAGAACGATTGCTGATACTGCTAAAGATAAAAAA‑GGAATCAGGCCATTAAGTAAAAACACCg > 1:1573921/1‑76 (MQ=255) cAATAGCACCTACAGAACGATTGCTGATACTGCTAAAGATAAAAAAGGGAATCAGGCCATTAAGTAAAAACACCgg < 1:137524/76‑1 (MQ=255) cAATAGCACCTACAGAACGATTGCTGATACTGCTAAAGATAAAAAA‑GGAATCAGGCCATTAAGTAAAAACACCggg > 1:352426/1‑76 (MQ=255) aTAGCACCTACAGAACGATTGCTGATACTGCTAAAGATAAAAAA‑GGAATCAGGCCATTAAGTAAAAACACCGGGaa < 2:1250476/76‑1 (MQ=255) tAGCACCTACAGAACGATTGCTGATACTGCTAAAGATAAAAAA‑GGAATCAGGCCATTAAGTAAAAACACCGGGAAt < 2:333109/76‑1 (MQ=255) aGCACCTACAGAACGATTGCTGATACTGCTAAAGATAAAAAA‑GGAATCAGGCCATTAAGTAAAAACACCGGGAATg < 2:743149/76‑1 (MQ=255) aGCACCTACAGAACGATTGCTGATACTGCTAAAGATAAAAAA‑GGAATCAGGCCATTAAGTAAAAACACCGGGAATg < 1:553512/76‑1 (MQ=255) gCACCTACAGAACGATTGCTGATACTGCTAAAGATAAAAAA‑GGAATCAGGCCATTAAGTAAAAACACCGGGaa > 1:681911/1‑73 (MQ=255) gCACCTACAGAACGATTGCTGATACTGCTAAAGATAAAAAA‑GGAATCAGGCCATTAAGTAAAAACACCGGGaa < 2:681911/73‑1 (MQ=255) aCCTACAGAACGATTGCTGATACTGCTAAAGATAAAAAA‑GGAATCAGGCCATTAAGTAAAAACACCGGGAATgaga > 1:1512532/1‑76 (MQ=255) cTACAGAACGATTGCTGATACTGCTAAAGATAAAAAA‑GGAATCAGGCCATTAAGTAAAAACACCGGGAATGAGATg < 2:1606243/76‑1 (MQ=255) cTACAGAACGATTGCTGATACTGCTAAAGATAAAAAA‑GGAATCAGGCCATTAAGTAAAAACACCGGGAATGAGATg < 2:959383/76‑1 (MQ=255) aCAGAACGATTGCTGATACTGCTAAAGATAAAAAA‑GGAATCAGGCCATTAAGTAAAAACACCGGGAATGAGATGTc > 2:1483515/1‑76 (MQ=255) cAGAACGATTGCTGATACTGCTAAAGATAAAAAA‑GGAATCAGGCCATTAAGTAAAAACACCGGGAATGAGATGTCt > 2:1148798/1‑76 (MQ=255) aGAACGATTGCTGATACTGCTAAAGATAAAAAA‑GGAATCAGGCCATTAAGTAAAAACACCGGGAATGAGATGTCTg < 2:785099/76‑1 (MQ=255) aaCGATTGCTGATACTGCTAAAGATAAAAAA‑GGAATCAGGCCATTAAGTAAAAACACCGGGAATGAGATGTCTGGc < 1:1267563/76‑1 (MQ=255) aaCGATTGCTGATACTGCTAAAGATAAAAAA‑GGAATCAGGCCATTAAGTAAAAACACCGGGAATGAGATGTCTGGc < 1:19557/76‑1 (MQ=255) aaCGATTGCTGATACTGCTAAAGATAAAAAA‑GGAATCAGGCCATTAAGTAAAAACACCGGGAATGAGATGTCTGGc < 1:224411/76‑1 (MQ=255) cGATTGCTGATACTGCTAAAGATAAAAAA‑GGAATCAGGCCATTAAGTAAAAACACCGGGAATGAGATGTCTGGCAt > 2:1237195/1‑76 (MQ=255) tGCTGATACTGCTAAAGATAAAAAA‑GGAATCAGGCCATTAAGTAAAAACACCGGGAATGAGATGTCTGGCATCGTg < 2:789332/76‑1 (MQ=255) tGCTGATACTGCTAAAGATAAAAAA‑GGAATCAGGCCATTAAGTAAAAACACCGGGAATGAGATGTCTGGCATCGTg < 2:246124/76‑1 (MQ=255) tGCTGATACTGCTAAAGATAAAAAA‑GGAATCAGGCCATTAAGTAAAAACACCGGGAATGAGATGTCTGGCATCGTg > 1:1117560/1‑76 (MQ=255) tGATACTGCTAAAGATAAAAAA‑GGAATCAGGCCATTAAGTAAAAACACCGGGAATGAGATGTCTGGCATCGTGCgg > 1:1095376/1‑76 (MQ=255) tACTGCTAAAGATAAAAAA‑GGAATCAGGCCATTAAGTAAAAACACCGGGAATGAGATGTCTGGCATCGTGCGGTGc < 1:455642/76‑1 (MQ=255) tGCTAAAGATAAAAAA‑GGAATCAGGCCATTAAGTATAAACACCGGGAATGAGATGTCTGGCATCGTGCGGTGCATa < 2:1421117/76‑1 (MQ=255) tGCTAAAGATAAAAAA‑GGAATCAGGCCATTAAGTAAAAACACCGGGAATGAGATGTCTGGCATCGTGCGGTGCATa < 1:1072184/76‑1 (MQ=255) cTAAAGATAAAAAA‑GGAATCAGGCCATTAAGTAAAAACACCGGGAATGAGATGTCTGGCATCGTGCGGTGcatcat > 1:272899/1‑76 (MQ=255) cTAAAGATAAAAAA‑GGAATCAGGCCATTAAGTAAAAACACCGGGAATGAGATGTCTGGCATCGTGCGGTGCATAAt > 1:272914/1‑76 (MQ=255) cTAAAGATAAAAAA‑GGAATCAGGCCATTAAGTAAAAACACCGGGAATGAGATGTCTGGCATCGTGCGGTGCATAAt > 1:1198994/1‑76 (MQ=255) cTAAAGATAAAAAA‑GGAATCAGGCCATTAAGTAAAAACACCGGGAATGAGATGTCTGGCATCGTGCGGTGCATAAt < 2:613476/76‑1 (MQ=255) tAAAGATAAAAAA‑GGAATCAGGCCATTAAGTAAAAACACCGGGAATGAGATGTCTGGCATCGTGCGGTGCATAATg < 2:55636/76‑1 (MQ=255) aaaGATAAAAAA‑GGAATCAGGCCATTAAGTAAAAACACCGGGAATGAGATGTCTGGCATCGTGCGGTGCATAATGt > 2:537796/1‑76 (MQ=255) aaaGATAAAAAA‑GGAATCAGGCCATTAAGTAAAAACACCGGGAATGAGATGTCTGGCATCGTGCGGTGCATAATGt < 2:268822/76‑1 (MQ=255) aaaGATAAAAAA‑GGAATCAGGCCATTAAGTAAAAACACCGGGAATGAGATGTCTGGCATCGTGCGGTGCATAATGt > 2:1534424/1‑76 (MQ=255) aaaGATAAAAAA‑GGAATCAGGCCATTAAGTAAAAACACCGGGAATGAGATGTCTGGCATCGTGCGGTGCATAATGt > 2:1115741/1‑76 (MQ=255) aGATAAAAAA‑GGAATCAGGCCATTAAGTAAAAACACCGGGAATGAGATGTCTGGCATCGTGCGGTGCATAATGTaa < 2:127363/76‑1 (MQ=255) ataaaaaaGGAATCAGGCCATTAAGTAAAAACACCGGGAATGAGATGTCTGGCATCGTGCGGTGCATAATGTAAcc < 2:462068/74‑1 (MQ=255) ataaaaaaGGAATCAGGCCATTAAGTAAAAACACCGGGAATGAGATGTCTGGCATCGTGCGGTGCATAATGTAAcc < 1:1054805/74‑1 (MQ=255) ataaaaaaGGAATCAGGCCATTAAGTAAAAACACCGGGAATGAGATGTCTGGCATCGTGCGGTGCATAATGTAAcc < 1:1647008/74‑1 (MQ=255) ataaaaaaGGAATCAGGCCATTAAGTAAAAACACCGGGAATGAGATGTCTGGCATCGTGCGGTGCATAATGTAAcc < 1:1054814/74‑1 (MQ=255) ataaaaaaGGAATCAGGCCATTAAGTAAAAACACCGGGAATGAGATGTCTGGCATCGTGCGGTGCATAATGTAAcc < 1:524131/74‑1 (MQ=255) ataaaaaaGGAATCAGGCCATTAAGTAAAAACACCGGGAATGAGATGTCTGGCATCGTGCGGTGCATAATGTAAcc > 1:825842/3‑76 (MQ=255) taaaaaaGGAATCAGGCCATTAAGTAAAAACACCGGGAATGAGATGTCTGGCATCGTGCGGTGCATAATGTAACCa < 1:433079/75‑1 (MQ=255) taaaaaaGGAATCAGGCCATTAAGTAAAAACACCGGGAATGAGATGTCTGGCATCGTGCGGTGCATAATGTAACCa > 2:779789/2‑76 (MQ=255) aaaaaaGGAATCAGGCCATTAAGTAAAAACACCGGGAATGAGATGTCTGGCATCGTGCGGTGCATAATGTAACCaa > 1:524043/1‑76 (MQ=255) aaaaAGGAATCAGGCCATTAAGTAAAAACACCGGGAATGAGATGTCTGGCATCGTGCGGTGCATAATGTAACCaaa > 2:689339/1‑76 (MQ=255) aaaaAGGAATCAGGCCATTAAGTAAAAACACCGGGAATGAGATGTCTGGCATCGTGCGGTGCATAATGTAACCaaa > 2:640516/1‑76 (MQ=255) aaaaAGGAATCAGGCCATTAAGTAAAAACACCGGGAATGAGATGTCTGGCATCGTGCGGTGCATAATGTAACCaaa > 2:760760/1‑76 (MQ=255) aaaaAGGAATCAGGCCATTAAGTAAAAACACCGGGAATGAGATGTCTGGCATCGTGCGGTGCATAATGTAACCaaa > 2:552595/1‑76 (MQ=255) | CGATAATTAAACAACCCCTGATTCGCTTCAATAGCACCTACAGAACGATTGCTGATACTGCTAAAGATAAAAAAGGGAATCAGGCCATTAAGTAAAAACACCGGGAATGAGATGTCTGGCATCGTGCGGTGCATAATGTAACCAAA > REL606/3028169‑3028314 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |