Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	1,182,792	A→G	intergenic (+60/+2)	ycfJ → / ← ycfQ	hypothetical protein/predicted DNA‑binding transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	1,182,792	0	A	G	100.0%	237.7 / NA	58	intergenic (+60/+2)	ycfJ/ycfQ	hypothetical protein/predicted DNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (35/23);  total (35/23)

TAACAAAGTATAACAAGGCTGTACTCTGCAATTTGGCCCCTCATTCGCTCAGGCTGAGGGGCTTTTTTTGCGACTTATTTCACCAGTTCGGGCCATAAACGCAAAGTCGTTCCGGCAATCTGCATTAGTTTTTCCAGCGATG  >  REL606/1182720‑1182861                                                                         |                                                                      tAACAAAGTATGACAAGGCTGTACTCTGCAATTTGGCCCCTCATTCGCTCAGGCTGAGGGGCTTTTTTTGCGGCtt                                                                    <  2:1492800/76‑1 (MQ=255)    cAAAGTATAACAAGGCTGTACTCTGCAATTTGGCCCCTCATTCGCTCAGGCTGAGGGGCTTTTTTTGCGGCTTAtt                                                                 <  2:1180585/76‑1 (MQ=255)    cAAAGTATAACAAGGCTGTACTCTGCAATTTGGCCCCTCATTCGCTCAGGCTGAGGGGCTTTTTTTGCGGCTTAtt                                                                 >  2:161337/1‑76 (MQ=255)     aaaGTATAACAAGGCTGTACTCTGCAATTTGGCCCCTCATTCGCTCAGGCTGAGGGGCTTTTTTTGCGGCTTAttt                                                                <  2:212650/76‑1 (MQ=255)      aaGTATAACAAGGCTGTACTCTGCAATTTGGCCCCTCATTCGCTCAGGCTGAGGGGCTTTTTTTGCGGCTTATTTc                                                               <  2:892414/76‑1 (MQ=255)       aGTATAACAAGGCTGTACTCTGCAATTTGGCCCCTCATTCGCTCAGGCTGAGGGGCTTTTTTTGCGGCTTATTTCa                                                              >  1:253844/1‑76 (MQ=255)         tataACAAGGCTGTACTCTGCAATTTGGCCCCTCATTCGCTCAGGCTGAGGGGCTTTTTTTGCGGCTTATTTCAcc                                                            <  2:1608664/76‑1 (MQ=255)         tataACAAGGCTGTACTCTGCAATTTGGCCCCTCATTCGCTCAGGCTGAGGGGCTTTTTTTGCGGCTTATTTCAcc                                                            >  2:503052/1‑76 (MQ=255)          ataACAAGGCTGTACTCTGCAATTTGGCCCCTCATTCGCTCACGCTGAGGGGCTTTTTTTGCGGCTTATTTCACCa                                                           >  2:143313/1‑76 (MQ=255)           taACAAGGCTGTACTCTGCAATTTGGCCCCTCATTCGCTCAGGCTGAGGGGCTTTTTTTGCGGCTTATTTCACCAg                                                          >  1:1323022/1‑76 (MQ=255)              cAAGGCTGTACTCTGCAATTTGGCCCCTCATTCGCTCAGGCTGAGGGGCTTTTTTTGCGGCTTATTTCACCAGTTc                                                       <  2:665818/76‑1 (MQ=255)               aaGGCTGTACTCTGCAATTTGGCCCCTCCTTCGCTCAGGCTGAGGGGCTTTTTTTGCGGCTTATTTCACCAGTTCg                                                      >  1:206730/1‑76 (MQ=255)               aaGGCTGTACTCTGCAATTTGGCCCCTCATTCGCTCAGGCTGAGGGGCTTTTTTTGCGGCTTATTTCACCAGTTCg                                                      >  1:1642934/1‑76 (MQ=255)               aaGGCTGTACTCTGCAATTTGGCCCCTCATTCGCTCAGGCTGAGGGGCTTTTTTTGCGGCTTATTTCACCAGTTCg                                                      >  1:187641/1‑76 (MQ=255)                  gCTGTACTCTGCAATTTGGCCCCTCATTCGCTCCGGCTGAGGGGCTTTTTTTGCGGCTTATTTCACCAGCTCGGGc                                                   >  2:839496/1‑76 (MQ=255)                   cTGTACTCTGCAATTTGGCCCCTCATTCGCTCAGGCTGAGGGGCTTTTTTTGCGGCTTATTTCACCAGTTCGGGcc                                                  <  1:1546524/76‑1 (MQ=255)                     gTACTCTGCAATTTGGCCCCTCATTCGCTCAGGCTGAGGGGCTTTTTTTGCGGCTTATTTCACCAGTTCGGGCCAt                                                >  2:984429/1‑76 (MQ=255)                      tACTCTGCAATTTGGCCCCTCATTCGCTCAGGCTGAGGGGCTTTTTTTGCGGCTTATTTCACCAGTTCGGGCCATa                                               >  1:1067512/1‑76 (MQ=255)                        ctctGCAATTTGGCCCCTCATTCGCTCAGGCTGAGGGGCTTTTTTTGCGGCTTATTTCACCAGTTCGGGCCATaaa                                             <  1:1476962/76‑1 (MQ=255)                        ctctGCAATTTGGCCCCTCATTCGCTCAGGCTGAGGGGCTTTTTTTGCGGCTTATTTCACCAGTTCGGGCCATaaa                                             >  2:957816/1‑76 (MQ=255)                        ctctGCAATTTGGCCCCTCATTCGCTCAGGCTGAGGGGCTTTTTTTGCGGCTTATTTCACCAGTTCGGGCCATaaa                                             >  2:1083202/1‑76 (MQ=255)                        ctctGCAATTTGGCCCCTCATTCGCTCAGGCTGAGGGGCTTTTTTTGCGGCTTATTTCA‑CAGGTCGGGCCCTAAAc                                            >  2:494383/1‑76 (MQ=255)                        ctctGCAATGTGGCCCCTCATTCGCTCAGGCTGAGGGGCTTTTTTTGCGGCTTATTTCACCAGTTCGGGCCATaaa                                             <  1:43328/76‑1 (MQ=255)                         tctGCAATTTGGCCCCTCATTCGCTCAGGCTGAGGGGCTTTTTTTGCGGCTTATTTCACCAGTTCGGGCCATAAAc                                            <  1:355981/76‑1 (MQ=255)                              aaTTTGGCCCCTCATTCGCTCAGGCTGAGGGTCTTTTTTTGCGGCTTATTTCACCTGTTCGGGCCATAAACGCaaa                                       >  2:726129/1‑76 (MQ=255)                              aaTTTGGCCCCTCATTCGCTCAGGCTGAGGGGCTTTTTTTGCGGCTTATTTCACCAGTTCGGGCCATAAACGCaaa                                       >  2:1269484/1‑76 (MQ=255)                               aTTTGGCCCCTCATTCGCTCAGGCTGAGGGGCTTTTTTTGCGGCTTATTTCACCAGTTCGGGCCATAAACGCAAAg                                      >  1:1041942/1‑76 (MQ=255)                                tttGGCCCCTCATTCGCTCAGGCTGAGGGGCTTTTTTTGCGGCTTATTTCACCAGTTCGGGCCATAAACGCAAAGt                                     >  2:1595949/1‑76 (MQ=255)                                  tGGCCCCTCATTCGCTCAGGCTGAGGGGCTTTTTTTGCGGCTTATTTCACCAGTTCGGGCCATAAACGCAAAGTCg                                   <  1:679987/76‑1 (MQ=255)                                    gCCCCTCATTCGCTCAGGCTGAGGGGCTTTTTTTGCGGCTTATTTCACCAGTTCGGGCCATAAACGCAAAGTCGtt                                 <  1:1001913/76‑1 (MQ=255)                                    gCCCCTCATTCGCTCAGGCTGAGGGGCTTTTTTTGCGGCTTATTTCACCAGTTCGGGCCATAAACGCAAAGTCGtt                                 >  2:781371/1‑76 (MQ=255)                                      cccTCATTCGCTCAGGCTGAGGGGCTTTTTTTGCGGCTTATTTCACCAGTTCGGGCCATAAACGCAAAGTCGTTcc                               >  1:345499/1‑76 (MQ=255)                                      cccTCATTCGCTCAGGCTGAGGGGCTTTTTTTGCGGCTTATTTCACCAGTTCGGGCCATAAACGCAAAGTCGTTcc                               >  2:290722/1‑76 (MQ=255)                                       ccTCATTCGCTCAGGCTGAGGGGCTTTTTTTGCGGCTTATTCCACCAGTTCGGGCCATAAACGCAAAGTCGTTCCg                              <  1:1596208/76‑1 (MQ=255)                                          cATTCGCTCAGGCTGAGGGGCTTTTTTTGCGGCTTATTTCACCAGTTCGGGCCATAAACGCAAAGTCGTTCCGGCa                           >  2:957110/1‑76 (MQ=255)                                          cATTCGCTCAGGCTGAGGGGCTTTTTTTGCGGCTTATTTCACCAGTTCGGGCCATAAACGCAAAGTCGTTCCGGCa                           <  2:344233/76‑1 (MQ=255)                                             tCGCTCAGGCTGAGGGGCTTTTTTTGCGGCTTATTTCACCAGTTCGGGCCATAAACGCAAAGTCGTTCCGGCAATc                        >  1:1408527/1‑76 (MQ=255)                                             tCGCTCAGGCTGAGGGGCTTTTTTTGCGGCTTATTTCACCAGTTCGGGCCATAAACGCAAAGTCGTTCCGGCAATc                        >  1:556407/1‑76 (MQ=255)                                              cGCTCAGGCTGAGGGGCTTTTTTTGCGGCTTATTTCACCAGTTCGGGCCATAAACGCAAAGTCGTTCCGGCAATCt                       >  1:972896/1‑76 (MQ=255)                                               gCTCAGGCTGAGGGGCTTTTTTTGCGGCTTATTTCACCAGTTCGGGCCATAAACGCAAAGTCGTTCCGGCAATCTg                      <  2:803241/76‑1 (MQ=255)                                               gCTCAGGCTGAGGGGCTTTTTTTGCGGCTTATTTCACCAGTTCGGGCCATAAACGCAAAGTCGTTCCGGCAATCTg                      <  2:1262608/76‑1 (MQ=255)                                               gCTCAGGCTGAGGGGCTTTTTTTGCGGCTTATTTCACCAGTTCGGGCCATAAACGCAAAGTCGTTCCGGCAATCTg                      <  2:1142469/76‑1 (MQ=255)                                               gCTCAGGCTGAGGGGCTTTTTTTGCGGCTTATTTCACCAGTTCGGGCCATAAACGCAAAGTCGTTCCGGCAATCTg                      <  2:1253626/76‑1 (MQ=255)                                               gCTCAGGCTGAGGGGCTTTTTTTGCGGCTTATTTCACCAGTTCGGGCCATAAACGCAAAGTCGTTCCGGCAATCTg                      <  2:759172/76‑1 (MQ=255)                                                cTCAGGCTGAGGGGCTTTTTTTGCGGCTTATTTCACCAGTTCGGGCCATAAACGCAAAGTCGTTCCGGCAATCTGc                     >  1:985615/1‑76 (MQ=255)                                                cTCAGGCTGAGGGGCTTTTTTTGCGGCTTATTTCACCAGTTCGGGCCATAAACGCAAAGTCGTTCCGGCAATCTGc                     <  1:388947/76‑1 (MQ=255)                                                  cAGGCTGAGGGGCTTTTTTTGCGGCTTATTTCACCAGTTCGGGCCATAAACGCAAAGTCGTTCCGGCAATCTGCAt                   >  2:664365/1‑76 (MQ=255)                                                   aGGCTGAGGGGCTTTTTTTGCGGCTTATTTCACCAGTTCGGGCCATAAACGCAAAGTCGTTCCGGCAATCTGCAtt                  >  1:129833/1‑76 (MQ=255)                                                    ggCTGAGGGGCTTTTTTTGCGGCTTATTTCACCAGTTCGGGCCATAAACGCAAAGTCGTTCCGGCAATCTGCATTa                 <  2:1343993/76‑1 (MQ=255)                                                        gAGGGGCTTTTTTTGCGGCTTATTTCACCAGTTCGGGCCATAAACGCAAAGTCGTTCCGGCAATCTGCATTAGttt             >  1:959641/1‑76 (MQ=255)                                                        gAGGGGCTTTTTTTGCGGCTTATTTCACCAGTTCGGGCCATAAACGCAAAGTCGTTCCGGCAATCTGCATTAGttt             >  2:67628/1‑76 (MQ=255)                                                        gAGGGGCTTTTTTTGCGGCTTATTTCACCAGTTCGGGCCATAAACGCAAAGTCGTTCCGGCAATCTGCATTAGttt             >  2:628644/1‑76 (MQ=255)                                                        gAGGGGCTTTTTTTGCGGCTTATTTCACCAGTTCGGGCCATAAACGCAAAGTCGTTCCGGCAATCTGCATTAGttt             >  2:626128/1‑76 (MQ=255)                                                        gAGGGGCTTTTTTTGCGGCTTATTTCACCAGTTCGGGCCATAAACGCAAAGTCGTTCCGGCAATCTGCATTAGttt             <  2:459036/76‑1 (MQ=255)                                                         aGGGGCTTTTTTTGCGGCTTATTTCACCAGTTCGGGCCATAAACGCAAAGTCGTTCCGGCAATCTGCATTAGtttt            >  2:959003/1‑76 (MQ=255)                                                             gCTTTTTTTGCGGCTTATTTCACCAGTTCGGGCCATAAACGCAAAGTCGTTCCGGCAATCTGCATTAGTTTTTCCa        <  1:11638/76‑1 (MQ=255)                                                                ttttttGCGGCTTATTTCACCAGTTCGGGCCATAAACGCAAAGTCGTTACGGCAATCTGCATTAGTTTTTCCAGCg     >  1:1495087/1‑76 (MQ=255)                                                                  ttttGCGGCTTATTTCACCAGTTCGGGCCATAAACGCAAAGTCGTTCCGGCAATCTGCATTAGTTTTTCCAGCGAt   >  2:738993/1‑76 (MQ=255)                                                                   tttGCGGCTTATTTCACCAGTTCGGGCCATAAACGCAAAGTCGTTCCGGCAATCTGCATTAGTTTTTCCAGCGATg  >  1:1424959/1‑76 (MQ=255)                                                                   tttGCGGCTTATTTCACCAGTTCGGGCCATAAACGCAAAGTCGTTCCGGCAATCTGCATTAGTTTTTCCAGCGATg  >  1:644764/1‑76 (MQ=255)                                                                   tttGCGGCTTATTTCACCAGTTCGGGCCATAAACGCAAAGTCGTTCCGGCAATCTGCATTAGTTTTTCCAGCGATg  >  2:1373122/1‑76 (MQ=255)                                                                         |                                                                      TAACAAAGTATAACAAGGCTGTACTCTGCAATTTGGCCCCTCATTCGCTCAGGCTGAGGGGCTTTTTTTGCGACTTATTTCACCAGTTCGGGCCATAAACGCAAAGTCGTTCCGGCAATCTGCATTAGTTTTTCCAGCGATG  >  REL606/1182720‑1182861

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑