Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	2,610,811	C→T	P329P (CCG→CCA)	yfhK ←	predicted sensory kinase in two‑component system

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	2,610,811	0	C	T	100.0%	205.4 / NA	52	P329P (CCG→CCA)	yfhK	predicted sensory kinase in two‑component system
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (23/29);  total (23/29)

TGAGATCGACGTCGGTATGCATCATTTTAGCCCGTGCGGGCAGGCTATGAGCAGAAACCACTGTCTCCACCAGCGGTGCTAATTCAACATTCTCCAGTTCCACCGCACTGTCCGCCTGTTTACGGTTGTAATCAAGCAGTTGT  >  REL606/2610738‑2610880                                                                          |                                                                      tGAGATCGACGTCGGTATGCATCATTTTAGCCCGTGCGGGCAGGCTATGAGCAGAAACCACTGTCTCCACCAGTgg                                                                     >  2:832603/1‑76 (MQ=255)   agaTCGACGTCGGTATGCATCATTTTAGCCCGTGCGGGCAGGCTATGAGCAGAAACCACTGTCTCCACCAgtggtg                                                                   <  2:204912/76‑1 (MQ=255)    gaTCGACGTCGGTATGCATCATTTTAGCCCGTGCGGGCAGGCTATGAGCAGAAACCACTGTCTCCACCAGTGGTGc                                                                  >  2:911733/1‑76 (MQ=255)     aTCGACGTCGGTATGCATCATTTTAGCCCGTGCGGGCAGGCTATGAGCAGAAACCACTGTCTCCACCAGTGGTGCt                                                                 >  2:1643741/1‑76 (MQ=255)     aTCGACGTCGGTACGCATCATTTTAGCCCGTGCGGGCAGGCTATGAGCAGAAACCACTGTCTCCACCAGTGGTGCt                                                                 <  1:803970/76‑1 (MQ=255)       cGACGTCGGTATGCATCATTTTAGCCCGTGCGGGCAGGCTATGAGCAGAAACCACTGTCTCCACCAGTGGTGCTaa                                                               >  2:1578112/1‑76 (MQ=255)           gTCGGTATGCATCATTTTAGCCCGTGCGGGCAGGCTATGAGCAGAAACCACTGTCTCCACCAGTGGTGCTAATTCa                                                           >  1:981846/1‑76 (MQ=255)                   gCATCATTTTAGCCCGTGCGGGCAGGCTATGAGCAGAAACCACTGTCTCCACCAGTGGTGCTAATTCAACATtctc                                                   >  1:1103419/1‑76 (MQ=255)                    catcatTTTAGCCCGTGCGGGCAGGCTATGAGCAGAAACCACTGTCTCCACCAGTGGTGCTAATTCAACATTCTcc                                                  <  2:826596/76‑1 (MQ=255)                          tttAGCCCGTGCGGGCAGGCTATGAGCAGAAACCACTGTCTCCACCAGTGGTGCTAATTCAACATTCTCCAGTTcc                                            <  1:1284874/76‑1 (MQ=255)                          tttAGCCCGTGCGGGCAGGCTATGAGCAGAAACCACTGTCTCCACCAGTGGTGCTAATTCAACATTCTCCAGTTcc                                            >  2:891669/1‑76 (MQ=255)                          tttAGCCCGTGCGGGCAGGCTATGAGCAGAAACCACTGTCTCCACCAGTGGTGCTAATTCAACATTCTCCAGTTcc                                            >  1:817474/1‑76 (MQ=255)                           ttAGCCCGTGCGGGCAGGCTATGAGCAGAAACCACTGTCTCCACCAGTGGTGCTAATTCAACATTCTCCAGTTCCa                                           >  1:1116980/1‑76 (MQ=255)                            tAGCCCGTGCGGGCAGGCTATGAGCAGAAACCACTGTCTCCACCAGTGGTGCTAATTCAACATTCTCCAGTTCCAc                                          >  1:1241379/1‑76 (MQ=255)                                  gTGCGGGCAGGCTATGAGCAGAAACCACTGTCTCCACCAGTGGTGCTAATTCAACATTCTCCAGTTCCACCGCACt                                    >  2:1100175/1‑76 (MQ=255)                                         cAGGCTATGAGCAGAAACCACTGTCTCCACCAGTGGTGCTAATTCAACATTCTCCAGTTCCACCGCACTGTCCGcc                             >  2:996858/1‑76 (MQ=255)                                           ggCTATGAGCAGAAACCACTGTCTCCACCAGTGGTGCTAATTCAACATTCTCCAGTTCCACCGCACTGTCCGCCTg                           <  2:799627/76‑1 (MQ=255)                                           ggCTATGAGCAGAAACCACTGTCTCCACCAGTGGTGCTAATTCAACATTCTCCAGTTCCACCGCACTGTCCGCCTg                           <  2:464507/76‑1 (MQ=255)                                             cTATGAGCAGAAACCACTGTCTCCACCAGTGGTGCTAATTCAACATTCTCCAGTTCCACCGCACTGTCCGCCTGtt                         >  1:974427/1‑76 (MQ=255)                                             cTATGAGCAGAAACCACTGTCTCCACCAGTGGTGCTAATTCAACATTCTCCAGTTCCACCGCACTGTCCGCCTCtt                         >  1:1282062/1‑76 (MQ=255)                                              tATGAGCAGAAACCACTGTCTCCACCAGTGGTGCTAATTCAACATTCTCCAGTTCCACCGCACTGTCCGCCTGttt                        <  2:275369/76‑1 (MQ=255)                                              tATGAGCAGAAACCACTGTCTCCACCAGTGGTGCTAATTCAACATTCTCCAGTTCCACCGCACTGTCCGCCTGttt                        <  2:1580841/76‑1 (MQ=255)                                               aTGAGCAGAAACCACTGTCTCCACCAGTGGTGCTAATTCAACATTCTCCAGTTCCACCGCACTGTCCGCCTGTTTa                       >  1:748176/1‑76 (MQ=255)                                               aTGAGCAGAAACCACTGTCTCCACCAGTGGTGCTAATTCAACATTCTCCAGTTCCACCGCACTGTCCGCCTGTTTa                       >  2:732890/1‑76 (MQ=255)                                                tGAGCAGAAACCACTGTCTCCACCAGTGGTGCTAATTCAACATTCTCCAGTTCCACCGCACTGTCCGCCTGTTTAc                      <  2:571201/76‑1 (MQ=255)                                                 gAGCAGAAACCACTGTCTCCACCAGTGGTGCTAATTCAACATTCTCCAGTTCCACCGCACTGTCCGCCTGTTTACg                     >  1:1273032/1‑76 (MQ=255)                                                 gAGCAGAAACCACTGTCTCCACCAGTGGTGCTAATTCAACATTCTCCAGTTCCACCGCACTGTCCGCCTGTTTACg                     >  1:174117/1‑76 (MQ=255)                                                   gCAGAAACCACTGTCTCCACCAGTGGTGCTAATTCAACATTCTCCAGTTCCACCGCACTGTCCGCCTGTTTACGGt                   <  1:287351/76‑1 (MQ=255)                                                   gCAGAAACCACTGTCTCCACCAGTGGTGCTAATTCAACATTCTCCAGTTCCACCGCACTGTCCGCCTGTTTACGGt                   <  1:974244/76‑1 (MQ=255)                                                   gCAGAAACCACTGTCTCCACCAGTGGTGCTAATTCAACATTCTCCAGTTCCACCGCACTGTCCGCCTGTTTACGGt                   <  1:1596466/76‑1 (MQ=255)                                                   gCAGAAACCACTGTCTCCACCAGTGGTGCTAATTCAACATTCTCCAGTTCCACCGCACTGTCCGCCTGTTTACGGt                   <  1:3419/76‑1 (MQ=255)                                                     aGAAACCACTGTCTCCACCAGTGGTGCTAATTCAACATTCTCCAGTTCCACCGCACTGTCCGCCTGTTTACGGTTg                 <  2:347673/76‑1 (MQ=255)                                                       aaaCCACTGTCTCCACCAGTGGTGCTAATTCAACATTCTCCAGTTCCACCGCACTGTCCGCCTGTTTACGGTTGTa               >  2:1496662/1‑76 (MQ=255)                                                       aaaCCACTGTCTCCACCAGTGGTGCTAATTCAACATTCTCCAGTTCCACCGCACTGTCCGCCTGTTTACGGTTGTa               >  2:891977/1‑76 (MQ=255)                                                        aaCCACTGTCTCCACCAGTGGTGCTAATTCAACATTCTCCAGTTCCACCGCACTGTCCGCCTGTTTACGGTTGTaa              <  1:147240/76‑1 (MQ=255)                                                         aCCACTGTCTCCACCAGTGGTGCTAATTCAACATTCTCCAGTTCCACCGCACTGTCCGCCTGTTTACGGTTGTAAt             <  1:456017/76‑1 (MQ=255)                                                          ccACTGTCTCCACCAGTGGTGCTAATTCAACATTCTCCAGTTCCACCGCACTGTCCGCCTGTTTACGGTTGTAATc            <  2:1404969/76‑1 (MQ=255)                                                          ccACTGTCTCCACCAGTGGTGCTAATTCAACATTCTCCAGTTCCACCGCACTGTCCGCCTGTTTACGGTTGTAATc            >  2:707526/1‑76 (MQ=255)                                                             cTGTCTCCACCAGTGGTGCTAATTCAACATTCTCCAGTTCCACCGCACTGTCCGCCTGTTTACGGTTGTAATCAAg         <  1:895333/76‑1 (MQ=255)                                                             cTGTCTCCACCAGTGGTGCTAATTCAACATTCTCCAGTTCCACCGCACTGTCCGCCTGTTTACGGTTGTAATCAAg         <  1:465632/76‑1 (MQ=255)                                                             cTGTCTCCACCAGTGGTGCTAATTCAACATTCTCCAGTTCCACCGCACTGTCCGCCTGTTTACGGTTGTAATCAAg         <  1:709362/76‑1 (MQ=255)                                                             cTGTCTCCACCAGTGGTGCTAATTCAACATTCTCCAGTTCCACCGCACTGTCCGCCTGTTTACGGTTGTAATCAAg         <  1:137673/76‑1 (MQ=255)                                                             cTGTCTCCACCAGTGGTGCTAATTCAACATTCTCCAGTTCCACCGCACTGTCCGCCTGTTTACGGTTGTAATCAAg         <  1:791308/76‑1 (MQ=255)                                                             cTGTCTCCACCAGTGGTGCTAATTCAACATTCTCCAGTTCCACCGCACTGTCCGCCTGTTTACGGTTGTAATCAAg         <  1:367404/76‑1 (MQ=255)                                                             cTGTCTCCACCAGTGGTGCTAATTCAACATTCTCCAGTTCCACCGCACTGTCCGCCTGTTTACGGTTGTAATCAAg         <  2:499491/76‑1 (MQ=255)                                                             cTGTCTCCACCAGTGGTGCTAATTCAACATTCTCCAGTTCCACCGCACTGTCCGCCTGTTTACGGTTGTAATCAAg         <  1:93289/76‑1 (MQ=255)                                                             cTGTCTCCACCAGTGGTGCTAATTCAACATTCTCCAGTTCCACCGCACTGTCCGCCTGTTTACGGTTGTAATCAAg         <  1:937441/76‑1 (MQ=255)                                                             cTGTCTCCACCAGTGGTGCTAATTCAACATTCTCCAGTTCCACCGCACTGTCCGCCTGTTTACGGTTGTAATCAAg         <  1:321120/76‑1 (MQ=255)                                                             cTGTCTCCACCAGTGGTGCTAATTCAACATTCTCCAGTTCCACCGCACTGTCCGCCTGTTTACGGTTGTAATCAAg         <  1:986879/76‑1 (MQ=255)                                                              tGTCTCCACCAGTGGTGCTAATTCAACATTCTCCAGTTCCACCGCACTGTCCGCCTGTTTACGGTTGTAATCAAGc        <  2:804620/76‑1 (MQ=255)                                                                   ccaccaGTGGTGCTAATTCAACATTCTCCAGTTCCACCGCACTGTCCGCCTGTTTACGGTTGTAATCAAGCAgttg   >  2:333955/1‑76 (MQ=255)                                                                   ccaccaGTGGTGCTAATTCAACATTCTCCAGTTCCACCGCACTGTCCGCCTGTTTACGGTTGTAATCAAGCAgttg   >  2:1529965/1‑76 (MQ=255)                                                                    caccaGTGGTGCTAATTCAACATTCTCCAGTTCCACCGCACTGTCCGCCTGTTTACGGTTGTAATCAAGCAgttgt  <  1:1595386/76‑1 (MQ=255)                                                                          |                                                                      TGAGATCGACGTCGGTATGCATCATTTTAGCCCGTGCGGGCAGGCTATGAGCAGAAACCACTGTCTCCACCAGCGGTGCTAATTCAACATTCTCCAGTTCCACCGCACTGTCCGCCTGTTTACGGTTGTAATCAAGCAGTTGT  >  REL606/2610738‑2610880

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑